Reviewed, UniProtKB/Swiss-Prot P26813 (DNLI_ASFM2)

Names and origin

Protein names

Recommended name:
    DNA ligase
    EC=6.5.1.1
Alternative name(s):
    Polydeoxyribonucleotide synthase [ATP]

Gene names

Name:	LIG
Ordered Locus Names:	Mal-108
ORF Names:	g3L

Organism

African swine fever virus (isolate Tick/Malawi/Lil 20-1/1983) (ASFV)

Taxonomic identifier

10500 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Asfarviridae › Asfivirus

Virus host

Ornithodoros (relapsing fever ticks) [TaxID: 6937]
Sus scrofa (Pig) [TaxID: 9823]
Phacochoerus africanus (Warthog) [TaxID: 41426]
Phacochoerus aethiopicus (Warthog) [TaxID: 85517]
Potamochoerus larvatus (Bushpig) [TaxID: 273792]

Protein attributes

Sequence length	419 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Very low-fidelity DNA ligase that seals, during DNA repair, nicks in double-stranded DNA. Together with the viral repair DNA polymerase X, fills the single nucleotide gaps generated by the AP endonuclease. It is not essential for viral replication and recombination By similarity.
Catalytic activity	ATP + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + diphosphate + (deoxyribonucleotide)(n+m).
Cofactor	Divalent metal cations By similarity.
Miscellaneous	Consistent with its intracellular location, ASFV encodes its own replicative DNA polymerase and three base excision repair enzymes: a class II AP endonuclease, the repair polymerase Pol X, and an ATP-dependent DNA ligase.
Sequence similarities	Belongs to the ATP-dependent DNA ligase family.

Ontologies

Keywords
Biological process	Cell cycle Cell division DNA damage DNA recombination DNA repair DNA replication
Ligand	ATP-binding Metal-binding Nucleotide-binding
Molecular function	Ligase
Gene Ontology (GO)
Biological process	DNA recombination Inferred from electronic annotation. Source: UniProtKB-KW DNA repair Inferred from electronic annotation. Source: UniProtKB-KW DNA replication Inferred from electronic annotation. Source: UniProtKB-KW cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA ligase (ATP) activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 419	419	DNA ligase		PRO_0000059594
Sites
	Active site	151	1	N6-AMP-lysine intermediate By similarity
	Metal binding	203	1	Divalent metal cation 1 By similarity
	Metal binding	291	1	Divalent metal cation 2 By similarity
	Binding site	156	1	ATP By similarity
	Binding site	172	1	ATP By similarity
	Binding site	203	1	ATP By similarity
	Binding site	232	1	ATP By similarity
	Binding site	316	1	ATP By similarity
Experimental info
	Sequence conflict	149 – 150	2	HG → QR Ref.3

Sequences

	Sequence		Length	Mass (Da)
	P26813-1 [UniParc]. Last modified August 1, 1992. Version 1. Checksum: DA781C64CA1B10F0	FASTA	419	48,041
	10 20 30 40 50 60 MLSQFPGQCS NNVFCFPPIE SETKNGKKAS WIICVQVMQH NTILPITDEM FSTDVKDAVA 70 80 90 100 110 120 EIFTKFFVEE GAVRISKTTR VTEGKNLGKK NATTVVHQAF KDALSKYNRH ARQKRGAHTN 130 140 150 160 170 180 RGMIPPMLVK YFNIIPKTFF EEETDPIVHG KRNGVRAVAC QQGDGSILLY SRTEKEFLGL 190 200 210 220 230 240 DNIKKELKQL YLFIDVRVYL DGELYLHRKP LQWIAGQANA KADSSELHFY VFDCFWSDQL 250 260 270 280 290 300 QMPSNKRQQL LTNIFKQKED LTFIHQVENF SVKNEDEALR LKTQFIKEGY EGAIVRNANG 310 320 330 340 350 360 PYEPGYNNYH SPHLAKLKPL LDAEFILVDY TQGKKGKDLG AILWVCELPN KKRFVVTPKH 370 380 390 400 410 LTYADRYALF QKLTPALFKK HLYGKELTVE YAELSPKTGI PLQARAVGFR EPINVLEII

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"An African swine fever virus gene with homology to DNA ligases." Hammond J.M., Kerr S.M., Smith G.L., Dixon L.K. Nucleic Acids Res. 20:2667-2671(1992) [PubMed: 1614852] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Nucleotide sequence of a 55 kbp region from the right end of the genome of a pathogenic African swine fever virus isolate (Malawi LIL20/1)." Dixon L.K., Twigg S.R.F., Baylis S.A., Vydelingum S., Bristow C., Hammond J.M., Smith G.L. J. Gen. Virol. 75:1655-1684(1994) [PubMed: 8021596] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"African swine fever virus genomes." Kutish G.F., Rock D.L. Submitted (MAR-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	X65192 Genomic DNA. Translation: CAA46310.1. X71982 Genomic DNA. Translation: CAA50805.1. AY261361 Genomic DNA. No translation available.
PIR	S23018.
3D structure databases
SMR	P26813. Positions 122-410.
ModBase	Search...
Family and domain databases
InterPro	IPR012310. DNA_ligase_A_M. IPR016059. DNA_ligase_CS. IPR016027. NA-bd_OB-fold-like. [Graphical view]
Pfam	PF01068. DNA_ligase_A_M. 1 hit. [Graphical view]
PROSITE	PS00697. DNA_LIGASE_A1. 1 hit. PS00333. DNA_LIGASE_A2. 1 hit. PS50160. DNA_LIGASE_A3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DNLI_ASFM2

Accession

Primary (citable) accession number: P26813

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1992
Last sequence update:	August 1, 1992
Last modified:	January 19, 2010
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Virus (Virus annotation project)