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Protein

DNA polymerase 1

Gene

dpo1

Organism
Sulfolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

This polymerase possesses two enzymatic activities: DNA synthesis (polymerase) and an exonucleolytic activity that degrades single-stranded DNA in the 3'- to 5'-direction. DNA polymerase I, DNA ligase and the flap endonuclease may be constitutively associated with the PCNA heterotrimer forming a scanning complex able to couple DNA synthesis and Okazaki fragment maturation.1 Publication

Catalytic activityi

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).

Enzyme regulationi

DNA synthesis is stimulated by PCNA heterotrimers.1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

DNA-directed DNA polymerase, Exonuclease, Hydrolase, Nuclease, Nucleotidyltransferase, Transferase

Keywords - Biological processi

DNA replication

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciSSOL273057:GCH2-531-MONOMER.
BRENDAi2.7.7.7. 6163.
3.1.11.1. 6163.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA polymerase 1 (EC:2.7.7.7)
Alternative name(s):
DNA polymerase I
Pol B1
Gene namesi
Name:dpo1
Synonyms:polS
Ordered Locus Names:SSO0552
OrganismiSulfolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)
Taxonomic identifieri273057 [NCBI]
Taxonomic lineageiArchaeaCrenarchaeotaThermoproteiSulfolobalesSulfolobaceaeSulfolobus
Proteomesi
  • UP000001974 Componenti: Chromosome

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi1 – 99Missing : No interaction with PCNA subunit PCNA2, DNA polymerase no longer stimulated by PCNA heterotrimers. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 882882DNA polymerase 1PRO_0000046483Add
BLAST

Interactioni

Subunit structurei

Interacts with PCNA subunit PCNA2 and weakly with PCNA3.1 Publication

Protein-protein interaction databases

DIPiDIP-48850N.
STRINGi273057.SSO0552.

Structurei

Secondary structure

1
882
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi50 – 5910Combined sources
Turni60 – 634Combined sources
Beta strandi64 – 718Combined sources
Turni72 – 754Combined sources
Beta strandi76 – 816Combined sources
Beta strandi89 – 946Combined sources
Helixi96 – 994Combined sources
Helixi103 – 1064Combined sources
Beta strandi111 – 12010Combined sources
Turni122 – 1243Combined sources
Beta strandi127 – 13711Combined sources
Helixi138 – 1447Combined sources
Beta strandi147 – 1493Combined sources
Beta strandi151 – 1533Combined sources
Helixi158 – 1669Combined sources
Beta strandi172 – 1776Combined sources
Beta strandi180 – 1834Combined sources
Helixi190 – 19910Combined sources
Turni200 – 2023Combined sources
Helixi205 – 21410Combined sources
Helixi216 – 2194Combined sources
Beta strandi227 – 2348Combined sources
Beta strandi237 – 2404Combined sources
Turni245 – 2473Combined sources
Beta strandi252 – 2598Combined sources
Beta strandi264 – 2696Combined sources
Beta strandi279 – 2813Combined sources
Beta strandi284 – 2918Combined sources
Helixi292 – 30312Combined sources
Beta strandi307 – 3137Combined sources
Turni314 – 3174Combined sources
Helixi318 – 32710Combined sources
Turni328 – 3303Combined sources
Helixi333 – 3353Combined sources
Beta strandi337 – 3393Combined sources
Beta strandi351 – 3555Combined sources
Helixi356 – 3605Combined sources
Helixi363 – 3686Combined sources
Helixi379 – 3879Combined sources
Turni398 – 4003Combined sources
Helixi403 – 41917Combined sources
Turni420 – 4223Combined sources
Helixi423 – 43917Combined sources
Helixi443 – 4464Combined sources
Helixi451 – 46616Combined sources
Helixi473 – 4786Combined sources
Beta strandi504 – 51512Combined sources
Helixi518 – 5258Combined sources
Turni530 – 5323Combined sources
Beta strandi547 – 5493Combined sources
Beta strandi553 – 5553Combined sources
Helixi562 – 57615Combined sources
Helixi578 – 5825Combined sources
Helixi589 – 61426Combined sources
Helixi623 – 64523Combined sources
Turni646 – 6483Combined sources
Beta strandi651 – 6555Combined sources
Beta strandi658 – 6636Combined sources
Helixi666 – 67914Combined sources
Beta strandi684 – 69512Combined sources
Beta strandi702 – 7054Combined sources
Beta strandi707 – 7093Combined sources
Beta strandi713 – 7153Combined sources
Helixi730 – 73910Combined sources
Helixi749 – 76214Combined sources
Helixi843 – 8475Combined sources
Helixi853 – 8575Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1S5JX-ray2.40A36-882[»]
ProteinModelPortaliP26811.
SMRiP26811. Positions 40-766.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP26811.

Family & Domainsi

Sequence similaritiesi

Belongs to the DNA polymerase type-B family.Curated

Phylogenomic databases

eggNOGiarCOG00328. Archaea.
COG0417. LUCA.
HOGENOMiHOG000108124.
InParanoidiP26811.
KOiK02319.
OMAiKGKKYAG.

Family and domain databases

Gene3Di3.30.420.10. 1 hit.
3.90.1600.10. 2 hits.
InterProiIPR006172. DNA-dir_DNA_pol_B.
IPR017964. DNA-dir_DNA_pol_B_CS.
IPR006133. DNA-dir_DNA_pol_B_exonuc.
IPR006134. DNA-dir_DNA_pol_B_multi_dom.
IPR023211. DNA_pol_palm_dom.
IPR012337. RNaseH-like_dom.
[Graphical view]
PfamiPF00136. DNA_pol_B. 1 hit.
PF03104. DNA_pol_B_exo1. 1 hit.
[Graphical view]
PRINTSiPR00106. DNAPOLB.
SMARTiSM00486. POLBc. 1 hit.
[Graphical view]
SUPFAMiSSF53098. SSF53098. 1 hit.
PROSITEiPS00116. DNA_POLYMERASE_B. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P26811-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTKQLTLFDI PSSKPAKSEQ NTQQSQQSAP VEEKKVVRRE WLEEAQENKI
60 70 80 90 100
YFLLQVDYDG KKGKAVCKLF DKETQKIYAL YDNTGHKPYF LVDLEPDKVG
110 120 130 140 150
KIPKIVRDPS FDHIETVSKI DPYTWNKFKL TKIVVRDPLA VRRLRNDVPK
160 170 180 190 200
AYEAHIKYFN NYMYDIGLIP GMPYVVKNGK LESVYLSLDE KDVEEIKKAF
210 220 230 240 250
ADSDEMTRQM AVDWLPIFET EIPKIKRVAI DIEVYTPVKG RIPDSQKAEF
260 270 280 290 300
PIISIALAGS DGLKKVLVLN RNDVNEGSVK LDGISVERFN TEYELLGRFF
310 320 330 340 350
DILLEYPIVL TFNGDDFDLP YIYFRALKLG YFPEEIPIDV AGKDEAKYLA
360 370 380 390 400
GLHIDLYKFF FNKAVRNYAF EGKYNEYNLD AVAKALLGTS KVKVDTLISF
410 420 430 440 450
LDVEKLIEYN FRDAEITLQL TTFNNDLTMK LIVLFSRISR LGIEELTRTE
460 470 480 490 500
ISTWVKNLYY WEHRKRNWLI PLKEEILAKS SNIRTSALIK GKGYKGAVVI
510 520 530 540 550
DPPAGIFFNI TVLDFASLYP SIIRTWNLSY ETVDIQQCKK PYEVKDETGE
560 570 580 590 600
VLHIVCMDRP GITAVITGLL RDFRVKIYKK KAKNPNNSEE QKLLYDVVQR
610 620 630 640 650
AMKVFINATY GVFGAETFPL YAPAVAESVT ALGRYVITST VKKAREEGLT
660 670 680 690 700
VLYGDTDSLF LLNPPKNSLE NIIKWVKTTF NLDLEVDKTY KFVAFSGLKK
710 720 730 740 750
NYFGVYQDGK VDIKGMLVKK RNTPEFVKKV FNEVKELMIS INSPNDVKEI
760 770 780 790 800
KRKIVDVVKG SYEKLKNKGY NLDELAFKVM LSKPLDAYKK NTPQHVKAAL
810 820 830 840 850
QLRPFGVNVL PRDIIYYVKV RSKDGVKPVQ LAKVTEIDAE KYLEALRSTF
860 870 880
EQILRAFGVS WDEIAATMSI DSFFSYPSKG NS
Length:882
Mass (Da):101,224
Last modified:December 1, 2000 - v2
Checksum:i77E3ED975AA1C81A
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti139 – 1391L → H in CAA45795 (PubMed:1614858).Curated
Sequence conflicti624 – 6241A → R in CAA45795 (PubMed:1614858).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X64466 Genomic DNA. Translation: CAA45795.1.
U92875 Genomic DNA. Translation: AAB53090.1.
Y18930 Genomic DNA. Translation: CAB57747.1.
AE006641 Genomic DNA. Translation: AAK40869.1.
PIRiF90201.
S23019.
RefSeqiWP_009991059.1. NC_002754.1.

Genome annotation databases

EnsemblBacteriaiAAK40869; AAK40869; SSO0552.
GeneIDi27426860.
KEGGisso:SSO0552.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X64466 Genomic DNA. Translation: CAA45795.1.
U92875 Genomic DNA. Translation: AAB53090.1.
Y18930 Genomic DNA. Translation: CAB57747.1.
AE006641 Genomic DNA. Translation: AAK40869.1.
PIRiF90201.
S23019.
RefSeqiWP_009991059.1. NC_002754.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1S5JX-ray2.40A36-882[»]
ProteinModelPortaliP26811.
SMRiP26811. Positions 40-766.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-48850N.
STRINGi273057.SSO0552.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAK40869; AAK40869; SSO0552.
GeneIDi27426860.
KEGGisso:SSO0552.

Phylogenomic databases

eggNOGiarCOG00328. Archaea.
COG0417. LUCA.
HOGENOMiHOG000108124.
InParanoidiP26811.
KOiK02319.
OMAiKGKKYAG.

Enzyme and pathway databases

BioCyciSSOL273057:GCH2-531-MONOMER.
BRENDAi2.7.7.7. 6163.
3.1.11.1. 6163.

Miscellaneous databases

EvolutionaryTraceiP26811.

Family and domain databases

Gene3Di3.30.420.10. 1 hit.
3.90.1600.10. 2 hits.
InterProiIPR006172. DNA-dir_DNA_pol_B.
IPR017964. DNA-dir_DNA_pol_B_CS.
IPR006133. DNA-dir_DNA_pol_B_exonuc.
IPR006134. DNA-dir_DNA_pol_B_multi_dom.
IPR023211. DNA_pol_palm_dom.
IPR012337. RNaseH-like_dom.
[Graphical view]
PfamiPF00136. DNA_pol_B. 1 hit.
PF03104. DNA_pol_B_exo1. 1 hit.
[Graphical view]
PRINTSiPR00106. DNAPOLB.
SMARTiSM00486. POLBc. 1 hit.
[Graphical view]
SUPFAMiSSF53098. SSF53098. 1 hit.
PROSITEiPS00116. DNA_POLYMERASE_B. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "A DNA polymerase from the archaeon Sulfolobus solfataricus shows sequence similarity to family B DNA polymerases."
    Pisani F.M., Martino C., Rossi M.
    Nucleic Acids Res. 20:2711-2716(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: DSM 5833 / MT-4.
  2. "Organizational characteristics and information content of an archaeal genome: 156 kb of sequence from Sulfolobus solfataricus P2."
    Sensen C.W., Klenk H.-P., Singh R.K., Allard G., Chan C.C.-Y., Liu Q.Y., Penny S.L., Young F., Schenk M.E., Gaasterland T., Doolittle W.F., Ragan M.A., Charlebois R.L.
    Mol. Microbiol. 22:175-191(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 35092 / DSM 1617 / JCM 11322 / P2.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 35092 / DSM 1617 / JCM 11322 / P2.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 35092 / DSM 1617 / JCM 11322 / P2.
  5. "A heterotrimeric PCNA in the hyperthermophilic archaeon Sulfolobus solfataricus."
    Dionne I., Nookala R.K., Jackson S.P., Doherty A.J., Bell S.D.
    Mol. Cell 11:275-282(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, ENZYME REGULATION, INTERACTION WITH PCNA3 AND PCNA2, SUBUNIT, MUTAGENESIS OF 1-MET--ASP-9.

Entry informationi

Entry nameiDPOL1_SULSO
AccessioniPrimary (citable) accession number: P26811
Secondary accession number(s): O05707
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: December 1, 2000
Last modified: July 6, 2016
This is version 129 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.