Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

DNA polymerase 1

Gene

dpo1

Organism
Sulfolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

This polymerase possesses two enzymatic activities: DNA synthesis (polymerase) and an exonucleolytic activity that degrades single-stranded DNA in the 3'- to 5'-direction. DNA polymerase I, DNA ligase and the flap endonuclease may be constitutively associated with the PCNA heterotrimer forming a scanning complex able to couple DNA synthesis and Okazaki fragment maturation.1 Publication

Catalytic activityi

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).

Enzyme regulationi

DNA synthesis is stimulated by PCNA heterotrimers.1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

DNA-directed DNA polymerase, Exonuclease, Hydrolase, Nuclease, Nucleotidyltransferase, Transferase

Keywords - Biological processi

DNA replication

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BRENDAi2.7.7.7. 6163.
3.1.11.1. 6163.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA polymerase 1 (EC:2.7.7.7)
Alternative name(s):
DNA polymerase I
Pol B1
Gene namesi
Name:dpo1
Synonyms:polS
Ordered Locus Names:SSO0552
OrganismiSulfolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)
Taxonomic identifieri273057 [NCBI]
Taxonomic lineageiArchaeaCrenarchaeotaThermoproteiSulfolobalesSulfolobaceaeSulfolobus
Proteomesi
  • UP000001974 Componenti: Chromosome

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi1 – 9Missing : No interaction with PCNA subunit PCNA2, DNA polymerase no longer stimulated by PCNA heterotrimers. 1 Publication9

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000464831 – 882DNA polymerase 1Add BLAST882

Interactioni

Subunit structurei

Interacts with PCNA subunit PCNA2 and weakly with PCNA3.1 Publication

Protein-protein interaction databases

DIPiDIP-48850N.
STRINGi273057.SSO0552.

Structurei

Secondary structure

1882
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi50 – 59Combined sources10
Turni60 – 63Combined sources4
Beta strandi64 – 71Combined sources8
Turni72 – 75Combined sources4
Beta strandi76 – 81Combined sources6
Beta strandi89 – 94Combined sources6
Helixi96 – 99Combined sources4
Helixi103 – 106Combined sources4
Beta strandi111 – 120Combined sources10
Turni122 – 124Combined sources3
Beta strandi127 – 137Combined sources11
Helixi138 – 144Combined sources7
Beta strandi147 – 149Combined sources3
Beta strandi151 – 153Combined sources3
Helixi158 – 166Combined sources9
Beta strandi172 – 177Combined sources6
Beta strandi180 – 183Combined sources4
Helixi190 – 199Combined sources10
Turni200 – 202Combined sources3
Helixi205 – 214Combined sources10
Helixi216 – 219Combined sources4
Beta strandi227 – 234Combined sources8
Beta strandi237 – 240Combined sources4
Turni245 – 247Combined sources3
Beta strandi252 – 259Combined sources8
Beta strandi264 – 269Combined sources6
Beta strandi279 – 281Combined sources3
Beta strandi284 – 291Combined sources8
Helixi292 – 303Combined sources12
Beta strandi307 – 313Combined sources7
Turni314 – 317Combined sources4
Helixi318 – 327Combined sources10
Turni328 – 330Combined sources3
Helixi333 – 335Combined sources3
Beta strandi337 – 339Combined sources3
Beta strandi351 – 355Combined sources5
Helixi356 – 360Combined sources5
Helixi363 – 368Combined sources6
Helixi379 – 387Combined sources9
Turni398 – 400Combined sources3
Helixi403 – 419Combined sources17
Turni420 – 422Combined sources3
Helixi423 – 439Combined sources17
Helixi443 – 446Combined sources4
Helixi451 – 466Combined sources16
Helixi473 – 478Combined sources6
Beta strandi504 – 515Combined sources12
Helixi518 – 525Combined sources8
Turni530 – 532Combined sources3
Beta strandi547 – 549Combined sources3
Beta strandi553 – 555Combined sources3
Helixi562 – 576Combined sources15
Helixi578 – 582Combined sources5
Helixi589 – 614Combined sources26
Helixi623 – 645Combined sources23
Turni646 – 648Combined sources3
Beta strandi651 – 655Combined sources5
Beta strandi658 – 663Combined sources6
Helixi666 – 679Combined sources14
Beta strandi684 – 695Combined sources12
Beta strandi702 – 705Combined sources4
Beta strandi707 – 709Combined sources3
Beta strandi713 – 715Combined sources3
Helixi730 – 739Combined sources10
Helixi749 – 762Combined sources14
Helixi843 – 847Combined sources5
Helixi853 – 857Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1S5JX-ray2.40A36-882[»]
ProteinModelPortaliP26811.
SMRiP26811.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP26811.

Family & Domainsi

Sequence similaritiesi

Belongs to the DNA polymerase type-B family.Curated

Phylogenomic databases

eggNOGiarCOG00328. Archaea.
COG0417. LUCA.
HOGENOMiHOG000108124.
InParanoidiP26811.
KOiK02319.
OMAiKGKKYAG.

Family and domain databases

Gene3Di3.30.420.10. 1 hit.
3.90.1600.10. 2 hits.
InterProiIPR006172. DNA-dir_DNA_pol_B.
IPR017964. DNA-dir_DNA_pol_B_CS.
IPR006133. DNA-dir_DNA_pol_B_exonuc.
IPR006134. DNA-dir_DNA_pol_B_multi_dom.
IPR023211. DNA_pol_palm_dom.
IPR012337. RNaseH-like_dom.
[Graphical view]
PfamiPF00136. DNA_pol_B. 1 hit.
PF03104. DNA_pol_B_exo1. 1 hit.
[Graphical view]
PRINTSiPR00106. DNAPOLB.
SMARTiSM00486. POLBc. 1 hit.
[Graphical view]
SUPFAMiSSF53098. SSF53098. 1 hit.
PROSITEiPS00116. DNA_POLYMERASE_B. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P26811-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTKQLTLFDI PSSKPAKSEQ NTQQSQQSAP VEEKKVVRRE WLEEAQENKI
60 70 80 90 100
YFLLQVDYDG KKGKAVCKLF DKETQKIYAL YDNTGHKPYF LVDLEPDKVG
110 120 130 140 150
KIPKIVRDPS FDHIETVSKI DPYTWNKFKL TKIVVRDPLA VRRLRNDVPK
160 170 180 190 200
AYEAHIKYFN NYMYDIGLIP GMPYVVKNGK LESVYLSLDE KDVEEIKKAF
210 220 230 240 250
ADSDEMTRQM AVDWLPIFET EIPKIKRVAI DIEVYTPVKG RIPDSQKAEF
260 270 280 290 300
PIISIALAGS DGLKKVLVLN RNDVNEGSVK LDGISVERFN TEYELLGRFF
310 320 330 340 350
DILLEYPIVL TFNGDDFDLP YIYFRALKLG YFPEEIPIDV AGKDEAKYLA
360 370 380 390 400
GLHIDLYKFF FNKAVRNYAF EGKYNEYNLD AVAKALLGTS KVKVDTLISF
410 420 430 440 450
LDVEKLIEYN FRDAEITLQL TTFNNDLTMK LIVLFSRISR LGIEELTRTE
460 470 480 490 500
ISTWVKNLYY WEHRKRNWLI PLKEEILAKS SNIRTSALIK GKGYKGAVVI
510 520 530 540 550
DPPAGIFFNI TVLDFASLYP SIIRTWNLSY ETVDIQQCKK PYEVKDETGE
560 570 580 590 600
VLHIVCMDRP GITAVITGLL RDFRVKIYKK KAKNPNNSEE QKLLYDVVQR
610 620 630 640 650
AMKVFINATY GVFGAETFPL YAPAVAESVT ALGRYVITST VKKAREEGLT
660 670 680 690 700
VLYGDTDSLF LLNPPKNSLE NIIKWVKTTF NLDLEVDKTY KFVAFSGLKK
710 720 730 740 750
NYFGVYQDGK VDIKGMLVKK RNTPEFVKKV FNEVKELMIS INSPNDVKEI
760 770 780 790 800
KRKIVDVVKG SYEKLKNKGY NLDELAFKVM LSKPLDAYKK NTPQHVKAAL
810 820 830 840 850
QLRPFGVNVL PRDIIYYVKV RSKDGVKPVQ LAKVTEIDAE KYLEALRSTF
860 870 880
EQILRAFGVS WDEIAATMSI DSFFSYPSKG NS
Length:882
Mass (Da):101,224
Last modified:December 1, 2000 - v2
Checksum:i77E3ED975AA1C81A
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti139L → H in CAA45795 (PubMed:1614858).Curated1
Sequence conflicti624A → R in CAA45795 (PubMed:1614858).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X64466 Genomic DNA. Translation: CAA45795.1.
U92875 Genomic DNA. Translation: AAB53090.1.
Y18930 Genomic DNA. Translation: CAB57747.1.
AE006641 Genomic DNA. Translation: AAK40869.1.
PIRiF90201.
S23019.
RefSeqiWP_009991059.1. NC_002754.1.

Genome annotation databases

EnsemblBacteriaiAAK40869; AAK40869; SSO0552.
GeneIDi27426860.
KEGGisso:SSO0552.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X64466 Genomic DNA. Translation: CAA45795.1.
U92875 Genomic DNA. Translation: AAB53090.1.
Y18930 Genomic DNA. Translation: CAB57747.1.
AE006641 Genomic DNA. Translation: AAK40869.1.
PIRiF90201.
S23019.
RefSeqiWP_009991059.1. NC_002754.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1S5JX-ray2.40A36-882[»]
ProteinModelPortaliP26811.
SMRiP26811.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-48850N.
STRINGi273057.SSO0552.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAK40869; AAK40869; SSO0552.
GeneIDi27426860.
KEGGisso:SSO0552.

Phylogenomic databases

eggNOGiarCOG00328. Archaea.
COG0417. LUCA.
HOGENOMiHOG000108124.
InParanoidiP26811.
KOiK02319.
OMAiKGKKYAG.

Enzyme and pathway databases

BRENDAi2.7.7.7. 6163.
3.1.11.1. 6163.

Miscellaneous databases

EvolutionaryTraceiP26811.

Family and domain databases

Gene3Di3.30.420.10. 1 hit.
3.90.1600.10. 2 hits.
InterProiIPR006172. DNA-dir_DNA_pol_B.
IPR017964. DNA-dir_DNA_pol_B_CS.
IPR006133. DNA-dir_DNA_pol_B_exonuc.
IPR006134. DNA-dir_DNA_pol_B_multi_dom.
IPR023211. DNA_pol_palm_dom.
IPR012337. RNaseH-like_dom.
[Graphical view]
PfamiPF00136. DNA_pol_B. 1 hit.
PF03104. DNA_pol_B_exo1. 1 hit.
[Graphical view]
PRINTSiPR00106. DNAPOLB.
SMARTiSM00486. POLBc. 1 hit.
[Graphical view]
SUPFAMiSSF53098. SSF53098. 1 hit.
PROSITEiPS00116. DNA_POLYMERASE_B. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDPOL1_SULSO
AccessioniPrimary (citable) accession number: P26811
Secondary accession number(s): O05707
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: December 1, 2000
Last modified: November 2, 2016
This is version 131 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.