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P26809

- POL_MLVFF

UniProt

P26809 - POL_MLVFF

Protein

Pol polyprotein

Gene

pol

Organism
Friend murine leukemia virus (isolate FB29) (FrMLV)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 101 (01 Oct 2014)
      Sequence version 1 (01 Aug 1992)
      Previous versions | rss
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    Functioni

    During replicative cycle of retroviruses, the reverse-transcribed viral DNA is integrated into the host chromosome by the viral integrase enzyme. RNase H activity is associated with the reverse transcriptase.

    Catalytic activityi

    Endonucleolytic cleavage to 5'-phosphomonoester.PROSITE-ProRule annotation
    Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).PROSITE-ProRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei32 – 321PROSITE-ProRule annotation

    GO - Molecular functioni

    1. aspartic-type endopeptidase activity Source: UniProtKB-KW
    2. RNA binding Source: InterPro
    3. RNA-directed DNA polymerase activity Source: UniProtKB-KW
    4. RNA-DNA hybrid ribonuclease activity Source: UniProtKB-EC

    GO - Biological processi

    1. DNA integration Source: UniProtKB-KW
    2. DNA recombination Source: UniProtKB-KW
    3. establishment of integrated proviral latency Source: UniProtKB-KW
    4. viral entry into host cell Source: UniProtKB-KW

    Keywords - Molecular functioni

    Aspartyl protease, Endonuclease, Hydrolase, Nuclease, Nucleotidyltransferase, Protease, RNA-directed DNA polymerase, Transferase

    Keywords - Biological processi

    DNA integration, DNA recombination, Viral genome integration, Virus entry into host cell

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Pol polyprotein
    Cleaved into the following 3 chains:
    Gene namesi
    Name:pol
    OrganismiFriend murine leukemia virus (isolate FB29) (FrMLV)
    Taxonomic identifieri11797 [NCBI]
    Taxonomic lineageiVirusesRetro-transcribing virusesRetroviridaeOrthoretrovirinaeGammaretrovirusMurine leukemia virus
    Virus hostiMus musculus (Mouse) [TaxID: 10090]
    ProteomesiUP000008877: Genome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini? – 1204IntegrasePRO_0000259730
    Chaini1 – 12041204Pol polyproteinPRO_0000259728Add
    BLAST
    Chaini1 – 125125ProteasePRO_0000026131Add
    BLAST
    Chaini126 – ?Reverse transcriptase/ribonuclease HPRO_0000259729

    Post-translational modificationi

    Specific enzymatic cleavages in vivo yield mature proteins.

    Structurei

    3D structure databases

    ProteinModelPortaliP26809.
    SMRiP26809. Positions 149-599, 628-793.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini27 – 9771Peptidase A2PROSITE-ProRule annotationAdd
    BLAST
    Domaini207 – 398192Reverse transcriptasePROSITE-ProRule annotationAdd
    BLAST
    Domaini640 – 786147RNase HPROSITE-ProRule annotationAdd
    BLAST
    Domaini910 – 1068159Integrase catalyticPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the retroviral Pol polyprotein family.Curated
    Contains 1 integrase catalytic domain.PROSITE-ProRule annotation
    Contains 1 peptidase A2 domain.PROSITE-ProRule annotation
    Contains 1 reverse transcriptase domain.PROSITE-ProRule annotation
    Contains 1 RNase H domain.PROSITE-ProRule annotation

    Family and domain databases

    Gene3Di2.40.70.10. 1 hit.
    3.30.420.10. 2 hits.
    InterProiIPR001969. Aspartic_peptidase_AS.
    IPR001584. Integrase_cat-core.
    IPR018061. Pept_A2A_retrovirus_sg.
    IPR001995. Peptidase_A2_cat.
    IPR021109. Peptidase_aspartic_dom.
    IPR012337. RNaseH-like_dom.
    IPR002156. RNaseH_domain.
    IPR000477. RT_dom.
    [Graphical view]
    PfamiPF00075. RNase_H. 1 hit.
    PF00665. rve. 1 hit.
    PF00077. RVP. 1 hit.
    PF00078. RVT_1. 1 hit.
    [Graphical view]
    SUPFAMiSSF50630. SSF50630. 1 hit.
    SSF53098. SSF53098. 2 hits.
    PROSITEiPS50175. ASP_PROT_RETROV. 1 hit.
    PS00141. ASP_PROTEASE. 1 hit.
    PS50994. INTEGRASE. 1 hit.
    PS50879. RNASE_H. 1 hit.
    PS50878. RT_POL. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P26809-1 [UniParc]FASTAAdd to Basket

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    TLDDQGGQGQ EPPPEPRITL RVGGQPVTFL VDTGAQHSVL TQNPGPLSDK     50
    SAWVQGATGG KRYRWTTDRR VHLATGKVTH SFLHVPDCPY PLLGRDLLTK 100
    LKAQIHFEGS GAQVVGPMGQ PLQVLTLNIE DEYRLHETSK GPDVPLGSTW 150
    LSDFPQAWAE TGGMGLAVRQ APLIIPLKAT STPVSIKQYP MSQEARLGIK 200
    PHIQRLLDQG ILVPCQSPWN TPLLPVKKPG TNDYRPVQDL REVNKRVEDI 250
    HPTVPNPYNL LSGLPPSHQW YTVLDLKDAF FCLRLHPTSQ SLFAFEWRDP 300
    EMGISGQLTW TRLPQGFKNS PTLFDEALHR DLADFRIQHP DLILLQYVDD 350
    LLLAATSELD CQQGTRALLQ TLGDLGYRAS AKKAQICQKQ VKYLGYLLKE 400
    GQRWLTEARK ETVMGQPTPK TPRQLREFLG TAGFCRLWIP GFAEMAAPLY 450
    PLTKTGTLFE WGPDQQKAYQ EIKQALLTAP ALGLPDLTKP FELFVDEKQG 500
    YAKGVLTQKL GPWRRPVAYL SKKLDPVAAG WPPCLRMVAA IAVLTKDAGK 550
    LTMGQPLVIL APHAVEALVK QPPDRWLSNA RMTHYQALLL DTDRVQFGPI 600
    VALNPATLLP LPEEGLQHDC LDILAEAHGT RPDLTDQPLP DADHTWYTDG 650
    SSFLQEGQRK AGAAVTTETE VVWAKALPAG TSAQRAELIA LTQALKMAEG 700
    KKLNVYTDSR YAFATAHIHG EIYRRRGLLT SEGKEIKNKD EILALLKALF 750
    LPKRLSIIHC PGHQKGNRAE ARGNRMADQA AREVATRETP ETSTLLIENS 800
    APYTHEHFHY TVTDIKDLTK LGATYDDAKK CWVYQGKPVM PDQFTFELLD 850
    FLHQLTHLSF SKTKALLERN YCPYYMLNRD RTLKDITETC QACAQVNASK 900
    SAVKQGTRVR GHRPGTHWEI DFTEVKPGLY GYKYLLVFID TFSGWVEAFP 950
    TKKETAKVVT KKLLEEIFPR FGMPQVLGTD NGPAFVSKVS QTVADLLGVD 1000
    WKLHCAYRPQ SSGQVERMNR TIKETLTKLT LATGSRDWVL LLPLALYRAR 1050
    NTPGPHGLTP YEILYGAPPP LVNFPDPDMA KVTHNPSLQA HLQALYLVQH 1100
    EVWRPLAAAY QEQLDRPVVP HPFRVGDTVW VRRHQTKNLE PRWKGPYTVL 1150
    LTTPTALKVD GIAAWIHAAH VKAADTRIEP PSESTWRVQR SQNPLKIRLT 1200
    RGTS 1204
    Length:1,204
    Mass (Da):134,706
    Last modified:August 1, 1992 - v1
    Checksum:iC046D8501CA7F64A
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z11128 Genomic DNA. Translation: CAA77477.1.
    PIRiS70393.
    RefSeqiNP_040333.1. NC_001362.1.

    Genome annotation databases

    GeneIDi1491877.

    Keywords - Coding sequence diversityi

    RNA suppression of termination

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z11128 Genomic DNA. Translation: CAA77477.1 .
    PIRi S70393.
    RefSeqi NP_040333.1. NC_001362.1.

    3D structure databases

    ProteinModelPortali P26809.
    SMRi P26809. Positions 149-599, 628-793.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 1491877.

    Family and domain databases

    Gene3Di 2.40.70.10. 1 hit.
    3.30.420.10. 2 hits.
    InterProi IPR001969. Aspartic_peptidase_AS.
    IPR001584. Integrase_cat-core.
    IPR018061. Pept_A2A_retrovirus_sg.
    IPR001995. Peptidase_A2_cat.
    IPR021109. Peptidase_aspartic_dom.
    IPR012337. RNaseH-like_dom.
    IPR002156. RNaseH_domain.
    IPR000477. RT_dom.
    [Graphical view ]
    Pfami PF00075. RNase_H. 1 hit.
    PF00665. rve. 1 hit.
    PF00077. RVP. 1 hit.
    PF00078. RVT_1. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50630. SSF50630. 1 hit.
    SSF53098. SSF53098. 2 hits.
    PROSITEi PS50175. ASP_PROT_RETROV. 1 hit.
    PS00141. ASP_PROTEASE. 1 hit.
    PS50994. INTEGRASE. 1 hit.
    PS50879. RNASE_H. 1 hit.
    PS50878. RT_POL. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Complete nucleotide sequence of Friend murine leukemia virus, strain FB29."
      Perryman S., Nishio J., Chesebro B.
      Nucleic Acids Res. 19:6950-6950(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

    Entry informationi

    Entry nameiPOL_MLVFF
    AccessioniPrimary (citable) accession number: P26809
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1992
    Last sequence update: August 1, 1992
    Last modified: October 1, 2014
    This is version 101 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    This protein is synthesized as a Gag-Pol polyprotein.

    Keywords - Technical termi

    Complete proteome, Multifunctional enzyme

    Documents

    1. Peptidase families
      Classification of peptidase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3