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P26809

- POL_MLVFF

UniProt

P26809 - POL_MLVFF

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Protein

Pol polyprotein

Gene

pol

Organism
Friend murine leukemia virus (isolate FB29) (FrMLV)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi

Functioni

During replicative cycle of retroviruses, the reverse-transcribed viral DNA is integrated into the host chromosome by the viral integrase enzyme. RNase H activity is associated with the reverse transcriptase.

Catalytic activityi

Endonucleolytic cleavage to 5'-phosphomonoester.PROSITE-ProRule annotation
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei32 – 321PROSITE-ProRule annotation

GO - Molecular functioni

  1. aspartic-type endopeptidase activity Source: UniProtKB-KW
  2. nucleic acid binding Source: InterPro
  3. RNA-directed DNA polymerase activity Source: UniProtKB-KW
  4. RNA-DNA hybrid ribonuclease activity Source: UniProtKB-EC

GO - Biological processi

  1. DNA integration Source: UniProtKB-KW
  2. DNA recombination Source: UniProtKB-KW
  3. establishment of integrated proviral latency Source: UniProtKB-KW
  4. viral entry into host cell Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Aspartyl protease, Endonuclease, Hydrolase, Nuclease, Nucleotidyltransferase, Protease, RNA-directed DNA polymerase, Transferase

Keywords - Biological processi

DNA integration, DNA recombination, Viral genome integration, Virus entry into host cell

Names & Taxonomyi

Protein namesi
Recommended name:
Pol polyprotein
Cleaved into the following 3 chains:
Gene namesi
Name:pol
OrganismiFriend murine leukemia virus (isolate FB29) (FrMLV)
Taxonomic identifieri11797 [NCBI]
Taxonomic lineageiVirusesRetro-transcribing virusesRetroviridaeOrthoretrovirinaeGammaretrovirusMurine leukemia virus
Virus hostiMus musculus (Mouse) [TaxID: 10090]
ProteomesiUP000008877: Genome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini? – 1204IntegrasePRO_0000259730
Chaini1 – 12041204Pol polyproteinPRO_0000259728Add
BLAST
Chaini1 – 125125ProteasePRO_0000026131Add
BLAST
Chaini126 – ?Reverse transcriptase/ribonuclease HPRO_0000259729

Post-translational modificationi

Specific enzymatic cleavages in vivo yield mature proteins.

Structurei

3D structure databases

ProteinModelPortaliP26809.
SMRiP26809. Positions 149-599, 628-793.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini27 – 9771Peptidase A2PROSITE-ProRule annotationAdd
BLAST
Domaini207 – 398192Reverse transcriptasePROSITE-ProRule annotationAdd
BLAST
Domaini640 – 786147RNase HPROSITE-ProRule annotationAdd
BLAST
Domaini910 – 1068159Integrase catalyticPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the retroviral Pol polyprotein family.Curated
Contains 1 integrase catalytic domain.PROSITE-ProRule annotation
Contains 1 peptidase A2 domain.PROSITE-ProRule annotation
Contains 1 reverse transcriptase domain.PROSITE-ProRule annotation
Contains 1 RNase H domain.PROSITE-ProRule annotation

Family and domain databases

Gene3Di2.40.70.10. 1 hit.
3.30.420.10. 2 hits.
InterProiIPR001969. Aspartic_peptidase_AS.
IPR001584. Integrase_cat-core.
IPR018061. Pept_A2A_retrovirus_sg.
IPR001995. Peptidase_A2_cat.
IPR021109. Peptidase_aspartic_dom.
IPR012337. RNaseH-like_dom.
IPR002156. RNaseH_domain.
IPR000477. RT_dom.
[Graphical view]
PfamiPF00075. RNase_H. 1 hit.
PF00665. rve. 1 hit.
PF00077. RVP. 1 hit.
PF00078. RVT_1. 1 hit.
[Graphical view]
SUPFAMiSSF50630. SSF50630. 1 hit.
SSF53098. SSF53098. 2 hits.
PROSITEiPS50175. ASP_PROT_RETROV. 1 hit.
PS00141. ASP_PROTEASE. 1 hit.
PS50994. INTEGRASE. 1 hit.
PS50879. RNASE_H. 1 hit.
PS50878. RT_POL. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P26809-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
TLDDQGGQGQ EPPPEPRITL RVGGQPVTFL VDTGAQHSVL TQNPGPLSDK
60 70 80 90 100
SAWVQGATGG KRYRWTTDRR VHLATGKVTH SFLHVPDCPY PLLGRDLLTK
110 120 130 140 150
LKAQIHFEGS GAQVVGPMGQ PLQVLTLNIE DEYRLHETSK GPDVPLGSTW
160 170 180 190 200
LSDFPQAWAE TGGMGLAVRQ APLIIPLKAT STPVSIKQYP MSQEARLGIK
210 220 230 240 250
PHIQRLLDQG ILVPCQSPWN TPLLPVKKPG TNDYRPVQDL REVNKRVEDI
260 270 280 290 300
HPTVPNPYNL LSGLPPSHQW YTVLDLKDAF FCLRLHPTSQ SLFAFEWRDP
310 320 330 340 350
EMGISGQLTW TRLPQGFKNS PTLFDEALHR DLADFRIQHP DLILLQYVDD
360 370 380 390 400
LLLAATSELD CQQGTRALLQ TLGDLGYRAS AKKAQICQKQ VKYLGYLLKE
410 420 430 440 450
GQRWLTEARK ETVMGQPTPK TPRQLREFLG TAGFCRLWIP GFAEMAAPLY
460 470 480 490 500
PLTKTGTLFE WGPDQQKAYQ EIKQALLTAP ALGLPDLTKP FELFVDEKQG
510 520 530 540 550
YAKGVLTQKL GPWRRPVAYL SKKLDPVAAG WPPCLRMVAA IAVLTKDAGK
560 570 580 590 600
LTMGQPLVIL APHAVEALVK QPPDRWLSNA RMTHYQALLL DTDRVQFGPI
610 620 630 640 650
VALNPATLLP LPEEGLQHDC LDILAEAHGT RPDLTDQPLP DADHTWYTDG
660 670 680 690 700
SSFLQEGQRK AGAAVTTETE VVWAKALPAG TSAQRAELIA LTQALKMAEG
710 720 730 740 750
KKLNVYTDSR YAFATAHIHG EIYRRRGLLT SEGKEIKNKD EILALLKALF
760 770 780 790 800
LPKRLSIIHC PGHQKGNRAE ARGNRMADQA AREVATRETP ETSTLLIENS
810 820 830 840 850
APYTHEHFHY TVTDIKDLTK LGATYDDAKK CWVYQGKPVM PDQFTFELLD
860 870 880 890 900
FLHQLTHLSF SKTKALLERN YCPYYMLNRD RTLKDITETC QACAQVNASK
910 920 930 940 950
SAVKQGTRVR GHRPGTHWEI DFTEVKPGLY GYKYLLVFID TFSGWVEAFP
960 970 980 990 1000
TKKETAKVVT KKLLEEIFPR FGMPQVLGTD NGPAFVSKVS QTVADLLGVD
1010 1020 1030 1040 1050
WKLHCAYRPQ SSGQVERMNR TIKETLTKLT LATGSRDWVL LLPLALYRAR
1060 1070 1080 1090 1100
NTPGPHGLTP YEILYGAPPP LVNFPDPDMA KVTHNPSLQA HLQALYLVQH
1110 1120 1130 1140 1150
EVWRPLAAAY QEQLDRPVVP HPFRVGDTVW VRRHQTKNLE PRWKGPYTVL
1160 1170 1180 1190 1200
LTTPTALKVD GIAAWIHAAH VKAADTRIEP PSESTWRVQR SQNPLKIRLT

RGTS
Length:1,204
Mass (Da):134,706
Last modified:August 1, 1992 - v1
Checksum:iC046D8501CA7F64A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z11128 Genomic DNA. Translation: CAA77477.1.
PIRiS70393.
RefSeqiNP_040333.1. NC_001362.1.

Genome annotation databases

GeneIDi1491877.

Keywords - Coding sequence diversityi

RNA suppression of termination

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z11128 Genomic DNA. Translation: CAA77477.1 .
PIRi S70393.
RefSeqi NP_040333.1. NC_001362.1.

3D structure databases

ProteinModelPortali P26809.
SMRi P26809. Positions 149-599, 628-793.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 1491877.

Family and domain databases

Gene3Di 2.40.70.10. 1 hit.
3.30.420.10. 2 hits.
InterProi IPR001969. Aspartic_peptidase_AS.
IPR001584. Integrase_cat-core.
IPR018061. Pept_A2A_retrovirus_sg.
IPR001995. Peptidase_A2_cat.
IPR021109. Peptidase_aspartic_dom.
IPR012337. RNaseH-like_dom.
IPR002156. RNaseH_domain.
IPR000477. RT_dom.
[Graphical view ]
Pfami PF00075. RNase_H. 1 hit.
PF00665. rve. 1 hit.
PF00077. RVP. 1 hit.
PF00078. RVT_1. 1 hit.
[Graphical view ]
SUPFAMi SSF50630. SSF50630. 1 hit.
SSF53098. SSF53098. 2 hits.
PROSITEi PS50175. ASP_PROT_RETROV. 1 hit.
PS00141. ASP_PROTEASE. 1 hit.
PS50994. INTEGRASE. 1 hit.
PS50879. RNASE_H. 1 hit.
PS50878. RT_POL. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Complete nucleotide sequence of Friend murine leukemia virus, strain FB29."
    Perryman S., Nishio J., Chesebro B.
    Nucleic Acids Res. 19:6950-6950(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Entry informationi

Entry nameiPOL_MLVFF
AccessioniPrimary (citable) accession number: P26809
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: August 1, 1992
Last modified: October 29, 2014
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Miscellaneous

This protein is synthesized as a Gag-Pol polyprotein.

Keywords - Technical termi

Complete proteome, Multifunctional enzyme

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3