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P26808 (POL_MLVFP) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 96. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Pol polyprotein

Cleaved into the following 3 chains:

  1. Protease
    EC=3.4.23.-
  2. Reverse transcriptase/ribonuclease H
    Short name=RT
    EC=2.7.7.49
    EC=3.1.26.4
  3. Integrase
    Short name=IN
Gene names
Name:pol
OrganismFriend murine leukemia virus (isolate PVC-211) (FrMLV) [Complete proteome]
Taxonomic identifier11798 [NCBI]
Taxonomic lineageVirusesRetro-transcribing virusesRetroviridaeOrthoretrovirinaeGammaretrovirusMurine leukemia virus
Virus hostMus musculus (Mouse) [TaxID: 10090]

Protein attributes

Sequence length1204 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

During replicative cycle of retroviruses, the reverse-transcribed viral DNA is integrated into the host chromosome by the viral integrase enzyme. RNase H activity is associated with the reverse transcriptase.

Catalytic activity

Endonucleolytic cleavage to 5'-phosphomonoester.

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).

Post-translational modification

Specific enzymatic cleavages in vivo yield mature proteins.

Miscellaneous

This protein is synthesized as a Gag-Pol polyprotein.

Sequence similarities

Belongs to the retroviral Pol polyprotein family.

Contains 1 integrase catalytic domain.

Contains 1 peptidase A2 domain.

Contains 1 reverse transcriptase domain.

Contains 1 RNase H domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 12041204Pol polyprotein
PRO_0000259731
Chain1 – 125125Protease
PRO_0000026132
Chain126 – ?Reverse transcriptase/ribonuclease HPRO_0000259732
Chain? – 1204IntegrasePRO_0000259733

Regions

Domain27 – 9771Peptidase A2
Domain207 – 398192Reverse transcriptase
Domain640 – 786147RNase H
Domain910 – 1068159Integrase catalytic

Sites

Active site321 By similarity

Sequences

Sequence LengthMass (Da)Tools
P26808 [UniParc].

Last modified August 1, 1992. Version 1.
Checksum: 8091F5C314D9798D

FASTA1,204134,694
        10         20         30         40         50         60 
TLDDQGGQGQ EPPPEPRITL KVGGQPVTFL VDTGAQHSVL TQNPGPLSDK SAWVQGATGG 

        70         80         90        100        110        120 
KRYRWTTDRR VHLATGKVTH SFLHVPDCPY PLLGRDLLTK LKAQIHFEGS GAQVVGPMGQ 

       130        140        150        160        170        180 
PLQVLTLNIE DEYRLHETSK GPDVPLGSTW LSDFPQAWAE TGGMGLAVRQ APLIIPLRAA 

       190        200        210        220        230        240 
STPVSIKQYP MSREARLGIK PHIQRLLDQG ILVPCQSPWN TPLLPVKKPG TNDYRPVQDL 

       250        260        270        280        290        300 
REVNKRVEDI HPTVPNPYNL LSGLPPSHQW YTVLDLKDAF FCLRLHPTSQ SLFAFEWRDP 

       310        320        330        340        350        360 
EMGISGQLTW TRLPQGFKNS PTLFDEALHR DLADFRIQHP DLILLQYVDD LLLAATSELD 

       370        380        390        400        410        420 
CQQGTRALLQ TLGDLGYRAS AKKAQICQKQ VKYLGYLLKE GQRWLTEARK ETVMGQPTPK 

       430        440        450        460        470        480 
TPRQLREFLG TAGFCRLWIP GFAEMAAPLY PLTKTGTLFK WGPDQQKAYQ EIKQALLTAP 

       490        500        510        520        530        540 
ALGLPDLTKP FELFVDEKQG YAKGVLTQKL GPWRRPVAYL SKKLDPVAAG WPPCLRMVAA 

       550        560        570        580        590        600 
IAVLTKDAGK LTMGQPLVIL APHAVEALVK QPPDRWLSNA RMTHYQALLL DTDRVQFGPI 

       610        620        630        640        650        660 
VTLNPATLLP LPEEGLQHDC LDILAEAHGT RPDLTDQPLP DADHTWYTDG SSFLQEGQRK 

       670        680        690        700        710        720 
AGAAVTTETE VIWAKALPAG TSAQRAELIA LTQALKMAEG KKLNVYTDSR YAFATAHIHG 

       730        740        750        760        770        780 
EIYRRRGLLT SEGKEIKNKE EILALLKALF LPKRLSIIHC PGHQKGNRAE ARGNRMADQA 

       790        800        810        820        830        840 
AREVATRETP ETSTLLIENS APYTREHFHY TVTDIKDLTK LGATYDNAQK CWVYQGKPVM 

       850        860        870        880        890        900 
PDQFTFELLD FLHQLTHLSF SKTKALLERS YSPYYMLNRD RTLKDITETC KACAQVNASK 

       910        920        930        940        950        960 
SAVKQGTRVR GHRPGTHWEI DFTEVKPGLY GYKYLLVFVD TFSGWVEAFP TKKETAKVVT 

       970        980        990       1000       1010       1020 
KKLLEEIFPR FGMPQVLGTD NGPAFVSKVS QTVADLLGVD WKLHCAYRPQ SSGQVERMNR 

      1030       1040       1050       1060       1070       1080 
TIKETLTKLT LATGSRDWVL LLPLALYRAR NTPGPHGLTP YEILYGAPPP LVNFPDPDMA 

      1090       1100       1110       1120       1130       1140 
KVTHNPSLQA HLQALYLVQH EVWRPLAAAY QEQLDRPVVP HPFRVGDTVW VRRHQTKNLE 

      1150       1160       1170       1180       1190       1200 
PRWKGPYTVL LTTPTALKVD GIAAWIHAAH VKAADTKIEP PSESTWRVQR SQNPLKIRLT 


RGTS 

« Hide

References

[1]"Complete nucleotide sequence of a neuropathogenic variant of Friend murine leukemia virus PVC-211."
Remington M.P., Hoffman P.M., Ruscetti S.K., Masuda M.
Nucleic Acids Res. 20:3249-3249(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M93134 Genomic RNA. Translation: AAA46477.1.
PIRS35475.

3D structure databases

ProteinModelPortalP26808.
SMRP26808. Positions 149-599, 628-793.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D2.40.70.10. 1 hit.
3.30.420.10. 2 hits.
InterProIPR001969. Aspartic_peptidase_AS.
IPR001584. Integrase_cat-core.
IPR018061. Pept_A2A_retrovirus_sg.
IPR001995. Peptidase_A2_cat.
IPR021109. Peptidase_aspartic_dom.
IPR012337. RNaseH-like_dom.
IPR002156. RNaseH_domain.
IPR000477. RT_dom.
[Graphical view]
PfamPF00075. RNase_H. 1 hit.
PF00665. rve. 1 hit.
PF00077. RVP. 1 hit.
PF00078. RVT_1. 1 hit.
[Graphical view]
SUPFAMSSF50630. SSF50630. 1 hit.
SSF53098. SSF53098. 2 hits.
PROSITEPS50175. ASP_PROT_RETROV. 1 hit.
PS00141. ASP_PROTEASE. 1 hit.
PS50994. INTEGRASE. 1 hit.
PS50879. RNASE_H. 1 hit.
PS50878. RT_POL. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePOL_MLVFP
AccessionPrimary (citable) accession number: P26808
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: August 1, 1992
Last modified: April 16, 2014
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries