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Protein

Pol polyprotein

Gene

pol

Organism
Friend murine leukemia virus (isolate PVC-211) (FrMLV)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

During replicative cycle of retroviruses, the reverse-transcribed viral DNA is integrated into the host chromosome by the viral integrase enzyme. RNase H activity is associated with the reverse transcriptase.

Catalytic activityi

Endonucleolytic cleavage to 5'-phosphomonoester.PROSITE-ProRule annotation
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei32 – 321PROSITE-ProRule annotation

GO - Molecular functioni

  1. aspartic-type endopeptidase activity Source: UniProtKB-KW
  2. nucleic acid binding Source: InterPro
  3. RNA-directed DNA polymerase activity Source: UniProtKB-KW
  4. RNA-DNA hybrid ribonuclease activity Source: UniProtKB-EC

GO - Biological processi

  1. DNA integration Source: UniProtKB-KW
  2. DNA recombination Source: UniProtKB-KW
  3. establishment of integrated proviral latency Source: UniProtKB-KW
  4. viral entry into host cell Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Aspartyl protease, Endonuclease, Hydrolase, Nuclease, Nucleotidyltransferase, Protease, RNA-directed DNA polymerase, Transferase

Keywords - Biological processi

DNA integration, DNA recombination, Viral genome integration, Virus entry into host cell

Names & Taxonomyi

Protein namesi
Recommended name:
Pol polyprotein
Cleaved into the following 3 chains:
Gene namesi
Name:pol
OrganismiFriend murine leukemia virus (isolate PVC-211) (FrMLV)
Taxonomic identifieri11798 [NCBI]
Taxonomic lineageiVirusesRetro-transcribing virusesRetroviridaeOrthoretrovirinaeGammaretrovirusMurine leukemia virus
Virus hostiMus musculus (Mouse) [TaxID: 10090]
ProteomesiUP000007777 Componenti: Genome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini? – 1204IntegrasePRO_0000259733
Chaini1 – 12041204Pol polyproteinPRO_0000259731Add
BLAST
Chaini1 – 125125ProteasePRO_0000026132Add
BLAST
Chaini126 – ?Reverse transcriptase/ribonuclease HPRO_0000259732

Post-translational modificationi

Specific enzymatic cleavages in vivo yield mature proteins.

Structurei

3D structure databases

ProteinModelPortaliP26808.
SMRiP26808. Positions 149-599, 628-793.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini27 – 9771Peptidase A2PROSITE-ProRule annotationAdd
BLAST
Domaini207 – 398192Reverse transcriptasePROSITE-ProRule annotationAdd
BLAST
Domaini640 – 786147RNase HPROSITE-ProRule annotationAdd
BLAST
Domaini910 – 1068159Integrase catalyticPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the retroviral Pol polyprotein family.Curated
Contains 1 integrase catalytic domain.PROSITE-ProRule annotation
Contains 1 peptidase A2 domain.PROSITE-ProRule annotation
Contains 1 reverse transcriptase domain.PROSITE-ProRule annotation
Contains 1 RNase H domain.PROSITE-ProRule annotation

Family and domain databases

Gene3Di2.40.70.10. 1 hit.
3.30.420.10. 2 hits.
InterProiIPR001969. Aspartic_peptidase_AS.
IPR001584. Integrase_cat-core.
IPR018061. Pept_A2A_retrovirus_sg.
IPR001995. Peptidase_A2_cat.
IPR021109. Peptidase_aspartic_dom.
IPR012337. RNaseH-like_dom.
IPR002156. RNaseH_domain.
IPR000477. RT_dom.
[Graphical view]
PfamiPF00075. RNase_H. 1 hit.
PF00665. rve. 1 hit.
PF00077. RVP. 1 hit.
PF00078. RVT_1. 1 hit.
[Graphical view]
SUPFAMiSSF50630. SSF50630. 1 hit.
SSF53098. SSF53098. 2 hits.
PROSITEiPS50175. ASP_PROT_RETROV. 1 hit.
PS00141. ASP_PROTEASE. 1 hit.
PS50994. INTEGRASE. 1 hit.
PS50879. RNASE_H. 1 hit.
PS50878. RT_POL. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P26808-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
TLDDQGGQGQ EPPPEPRITL KVGGQPVTFL VDTGAQHSVL TQNPGPLSDK
60 70 80 90 100
SAWVQGATGG KRYRWTTDRR VHLATGKVTH SFLHVPDCPY PLLGRDLLTK
110 120 130 140 150
LKAQIHFEGS GAQVVGPMGQ PLQVLTLNIE DEYRLHETSK GPDVPLGSTW
160 170 180 190 200
LSDFPQAWAE TGGMGLAVRQ APLIIPLRAA STPVSIKQYP MSREARLGIK
210 220 230 240 250
PHIQRLLDQG ILVPCQSPWN TPLLPVKKPG TNDYRPVQDL REVNKRVEDI
260 270 280 290 300
HPTVPNPYNL LSGLPPSHQW YTVLDLKDAF FCLRLHPTSQ SLFAFEWRDP
310 320 330 340 350
EMGISGQLTW TRLPQGFKNS PTLFDEALHR DLADFRIQHP DLILLQYVDD
360 370 380 390 400
LLLAATSELD CQQGTRALLQ TLGDLGYRAS AKKAQICQKQ VKYLGYLLKE
410 420 430 440 450
GQRWLTEARK ETVMGQPTPK TPRQLREFLG TAGFCRLWIP GFAEMAAPLY
460 470 480 490 500
PLTKTGTLFK WGPDQQKAYQ EIKQALLTAP ALGLPDLTKP FELFVDEKQG
510 520 530 540 550
YAKGVLTQKL GPWRRPVAYL SKKLDPVAAG WPPCLRMVAA IAVLTKDAGK
560 570 580 590 600
LTMGQPLVIL APHAVEALVK QPPDRWLSNA RMTHYQALLL DTDRVQFGPI
610 620 630 640 650
VTLNPATLLP LPEEGLQHDC LDILAEAHGT RPDLTDQPLP DADHTWYTDG
660 670 680 690 700
SSFLQEGQRK AGAAVTTETE VIWAKALPAG TSAQRAELIA LTQALKMAEG
710 720 730 740 750
KKLNVYTDSR YAFATAHIHG EIYRRRGLLT SEGKEIKNKE EILALLKALF
760 770 780 790 800
LPKRLSIIHC PGHQKGNRAE ARGNRMADQA AREVATRETP ETSTLLIENS
810 820 830 840 850
APYTREHFHY TVTDIKDLTK LGATYDNAQK CWVYQGKPVM PDQFTFELLD
860 870 880 890 900
FLHQLTHLSF SKTKALLERS YSPYYMLNRD RTLKDITETC KACAQVNASK
910 920 930 940 950
SAVKQGTRVR GHRPGTHWEI DFTEVKPGLY GYKYLLVFVD TFSGWVEAFP
960 970 980 990 1000
TKKETAKVVT KKLLEEIFPR FGMPQVLGTD NGPAFVSKVS QTVADLLGVD
1010 1020 1030 1040 1050
WKLHCAYRPQ SSGQVERMNR TIKETLTKLT LATGSRDWVL LLPLALYRAR
1060 1070 1080 1090 1100
NTPGPHGLTP YEILYGAPPP LVNFPDPDMA KVTHNPSLQA HLQALYLVQH
1110 1120 1130 1140 1150
EVWRPLAAAY QEQLDRPVVP HPFRVGDTVW VRRHQTKNLE PRWKGPYTVL
1160 1170 1180 1190 1200
LTTPTALKVD GIAAWIHAAH VKAADTKIEP PSESTWRVQR SQNPLKIRLT

RGTS
Length:1,204
Mass (Da):134,694
Last modified:July 31, 1992 - v1
Checksum:i8091F5C314D9798D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M93134 Genomic RNA. Translation: AAA46477.1.
PIRiS35475.

Keywords - Coding sequence diversityi

RNA suppression of termination

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M93134 Genomic RNA. Translation: AAA46477.1.
PIRiS35475.

3D structure databases

ProteinModelPortaliP26808.
SMRiP26808. Positions 149-599, 628-793.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di2.40.70.10. 1 hit.
3.30.420.10. 2 hits.
InterProiIPR001969. Aspartic_peptidase_AS.
IPR001584. Integrase_cat-core.
IPR018061. Pept_A2A_retrovirus_sg.
IPR001995. Peptidase_A2_cat.
IPR021109. Peptidase_aspartic_dom.
IPR012337. RNaseH-like_dom.
IPR002156. RNaseH_domain.
IPR000477. RT_dom.
[Graphical view]
PfamiPF00075. RNase_H. 1 hit.
PF00665. rve. 1 hit.
PF00077. RVP. 1 hit.
PF00078. RVT_1. 1 hit.
[Graphical view]
SUPFAMiSSF50630. SSF50630. 1 hit.
SSF53098. SSF53098. 2 hits.
PROSITEiPS50175. ASP_PROT_RETROV. 1 hit.
PS00141. ASP_PROTEASE. 1 hit.
PS50994. INTEGRASE. 1 hit.
PS50879. RNASE_H. 1 hit.
PS50878. RT_POL. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Complete nucleotide sequence of a neuropathogenic variant of Friend murine leukemia virus PVC-211."
    Remington M.P., Hoffman P.M., Ruscetti S.K., Masuda M.
    Nucleic Acids Res. 20:3249-3249(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Entry informationi

Entry nameiPOL_MLVFP
AccessioniPrimary (citable) accession number: P26808
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 31, 1992
Last sequence update: July 31, 1992
Last modified: January 6, 2015
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Miscellaneous

This protein is synthesized as a Gag-Pol polyprotein.

Keywords - Technical termi

Complete proteome, Multifunctional enzyme

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.