P26808 (POL_MLVFP) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Pol polyprotein

Cleaved into the following 3 chains:

1. Protease
   EC=3.4.23.-
2. Reverse transcriptase/ribonuclease H
   Short name=RT
   EC=2.7.7.49
   EC=3.1.26.4
3. Integrase
   Short name=IN

Gene names

Name:

pol

Organism

Friend murine leukemia virus (isolate PVC-211) (FrMLV) [Complete proteome]

Taxonomic identifier

11798 [NCBI]

Taxonomic lineage

Viruses › Retro-transcribing viruses › Retroviridae › Orthoretrovirinae › Gammaretrovirus › Murine leukemia virus

Virus host

Mus musculus (Mouse) [TaxID: 10090]

Protein attributes

Sequence length	1204 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	During replicative cycle of retroviruses, the reverse-transcribed viral DNA is integrated into the host chromosome by the viral integrase enzyme. RNase H activity is associated with the reverse transcriptase.
Catalytic activity	Endonucleolytic cleavage to 5'-phosphomonoester. Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).
Post-translational modification	Specific enzymatic cleavages in vivo yield mature proteins.
Miscellaneous	This protein is synthesized as a Gag-Pol polyprotein.
Sequence similarities	Belongs to the retroviral Pol polyprotein family. Contains 1 integrase catalytic domain. Contains 1 peptidase A2 domain. Contains 1 reverse transcriptase domain. Contains 1 RNase H domain.

Ontologies

Keywords
Biological process	DNA integration DNA recombination Initiation of viral infection Viral genome integration
Coding sequence diversity	RNA suppression of termination
Molecular function	Aspartyl protease Endonuclease Hydrolase Nuclease Nucleotidyltransferase Protease RNA-directed DNA polymerase Transferase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	DNA integration Inferred from electronic annotation. Source: UniProtKB-KW DNA recombination Inferred from electronic annotation. Source: UniProtKB-KW RNA-dependent DNA replication Inferred from electronic annotation. Source: InterPro proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	RNA binding Inferred from electronic annotation. Source: InterPro RNA-directed DNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW aspartic-type endopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW ribonuclease H activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1204	1204	Pol polyprotein		PRO_0000259731
	Chain	1 – 125	125	Protease		PRO_0000026132
	Chain	126 – ?		Reverse transcriptase/ribonuclease H		PRO_0000259732
	Chain	? – 1204		Integrase		PRO_0000259733
Regions
	Domain	27 – 97	71	Peptidase A2
	Domain	207 – 398	192	Reverse transcriptase
	Domain	640 – 786	147	RNase H
	Domain	910 – 1068	159	Integrase catalytic
Sites
	Active site	32	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	P26808 [UniParc]. Last modified August 1, 1992. Version 1. Checksum: 8091F5C314D9798D	FASTA	1,204	134,694
	10 20 30 40 50 60 TLDDQGGQGQ EPPPEPRITL KVGGQPVTFL VDTGAQHSVL TQNPGPLSDK SAWVQGATGG 70 80 90 100 110 120 KRYRWTTDRR VHLATGKVTH SFLHVPDCPY PLLGRDLLTK LKAQIHFEGS GAQVVGPMGQ 130 140 150 160 170 180 PLQVLTLNIE DEYRLHETSK GPDVPLGSTW LSDFPQAWAE TGGMGLAVRQ APLIIPLRAA 190 200 210 220 230 240 STPVSIKQYP MSREARLGIK PHIQRLLDQG ILVPCQSPWN TPLLPVKKPG TNDYRPVQDL 250 260 270 280 290 300 REVNKRVEDI HPTVPNPYNL LSGLPPSHQW YTVLDLKDAF FCLRLHPTSQ SLFAFEWRDP 310 320 330 340 350 360 EMGISGQLTW TRLPQGFKNS PTLFDEALHR DLADFRIQHP DLILLQYVDD LLLAATSELD 370 380 390 400 410 420 CQQGTRALLQ TLGDLGYRAS AKKAQICQKQ VKYLGYLLKE GQRWLTEARK ETVMGQPTPK 430 440 450 460 470 480 TPRQLREFLG TAGFCRLWIP GFAEMAAPLY PLTKTGTLFK WGPDQQKAYQ EIKQALLTAP 490 500 510 520 530 540 ALGLPDLTKP FELFVDEKQG YAKGVLTQKL GPWRRPVAYL SKKLDPVAAG WPPCLRMVAA 550 560 570 580 590 600 IAVLTKDAGK LTMGQPLVIL APHAVEALVK QPPDRWLSNA RMTHYQALLL DTDRVQFGPI 610 620 630 640 650 660 VTLNPATLLP LPEEGLQHDC LDILAEAHGT RPDLTDQPLP DADHTWYTDG SSFLQEGQRK 670 680 690 700 710 720 AGAAVTTETE VIWAKALPAG TSAQRAELIA LTQALKMAEG KKLNVYTDSR YAFATAHIHG 730 740 750 760 770 780 EIYRRRGLLT SEGKEIKNKE EILALLKALF LPKRLSIIHC PGHQKGNRAE ARGNRMADQA 790 800 810 820 830 840 AREVATRETP ETSTLLIENS APYTREHFHY TVTDIKDLTK LGATYDNAQK CWVYQGKPVM 850 860 870 880 890 900 PDQFTFELLD FLHQLTHLSF SKTKALLERS YSPYYMLNRD RTLKDITETC KACAQVNASK 910 920 930 940 950 960 SAVKQGTRVR GHRPGTHWEI DFTEVKPGLY GYKYLLVFVD TFSGWVEAFP TKKETAKVVT 970 980 990 1000 1010 1020 KKLLEEIFPR FGMPQVLGTD NGPAFVSKVS QTVADLLGVD WKLHCAYRPQ SSGQVERMNR 1030 1040 1050 1060 1070 1080 TIKETLTKLT LATGSRDWVL LLPLALYRAR NTPGPHGLTP YEILYGAPPP LVNFPDPDMA 1090 1100 1110 1120 1130 1140 KVTHNPSLQA HLQALYLVQH EVWRPLAAAY QEQLDRPVVP HPFRVGDTVW VRRHQTKNLE 1150 1160 1170 1180 1190 1200 PRWKGPYTVL LTTPTALKVD GIAAWIHAAH VKAADTKIEP PSESTWRVQR SQNPLKIRLT RGTS

References

[1]	"Complete nucleotide sequence of a neuropathogenic variant of Friend murine leukemia virus PVC-211." Remington M.P., Hoffman P.M., Ruscetti S.K., Masuda M. Nucleic Acids Res. 20:3249-3249(1992) [PubMed: 1620621] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M93134 Genomic RNA. Translation: AAA46477.1.
PIR	S35475.
3D structure databases
ProteinModelPortal	P26808.
SMR	P26808. Positions 149-599, 628-793.
ModBase	Search...
Protein family/group databases
MEROPS	A02.008.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR001584. Integrase_cat-core. IPR018061. Pept_A2A_retrovirus_sg. IPR001995. Peptidase_A2_cat. IPR021109. Peptidase_aspartic. IPR001969. Peptidase_aspartic_AS. IPR009007. Peptidase_aspartic_catalytic. IPR012337. RNaseH-like_dom. IPR002156. RNaseH_domain. IPR000477. RVT. [Graphical view]
Gene3D	G3DSA:2.40.70.10. Pept_Aspartc_cat. 1 hit.
Pfam	PF00075. RNase_H. 1 hit. PF00665. rve. 1 hit. PF00077. RVP. 1 hit. PF00078. RVT_1. 1 hit. [Graphical view]
SUPFAM	SSF50630. Pept_Aspartic. 1 hit. SSF53098. RNaseH_fold. 2 hits.
PROSITE	PS50175. ASP_PROT_RETROV. 1 hit. PS00141. ASP_PROTEASE. 1 hit. PS50994. INTEGRASE. 1 hit. PS50879. RNASE_H. 1 hit. PS50878. RT_POL. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

POL_MLVFP

Accession

Primary (citable) accession number: P26808

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1992
Last sequence update:	August 1, 1992
Last modified:	December 14, 2011
This is version 85 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program