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P26805

- GAG_MLVFP

UniProt

P26805 - GAG_MLVFP

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Protein
Gag polyprotein
Gene
gag
Organism
Friend murine leukemia virus (isolate PVC-211) (FrMLV)
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Gag polyprotein plays a role in budding and is processed by the viral protease during virion maturation outside the cell. During budding, it recruits, in a PPXY-dependent or independent manner, Nedd4-like ubiquitin ligases that conjugate ubiquitin molecules to Gag, or to Gag binding host factors. Interaction with HECT ubiquitin ligases probably link the viral protein to the host ESCRT pathway and facilitate release By similarity.
Matrix protein p15 targets Gag and gag-pol polyproteins to the plasma membrane via a multipartite membrane binding signal, that includes its myristoylated N-terminus. Also mediates nuclear localization of the preintegration complex By similarity.
Capsid protein p30 forms the spherical core of the virion that encapsulates the genomic RNA-nucleocapsid complex By similarity.
Nucleocapsid protein p10 is involved in the packaging and encapsidation of two copies of the genome. Binds with high affinity to conserved UCUG elements within the packaging signal, located near the 5'-end of the genome. This binding is dependent on genome dimerization By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei131 – 1322Cleavage; by viral protease p14 By similarity
Sitei215 – 2162Cleavage; by viral protease p14 By similarity
Sitei478 – 4792Cleavage; by viral protease p14 By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri502 – 51918CCHC-type
Add
BLAST

GO - Molecular functioni

  1. RNA binding Source: UniProtKB-KW
  2. structural constituent of virion Source: UniProtKB-KW
  3. zinc ion binding Source: InterPro

GO - Biological processi

  1. viral budding via host ESCRT complex Source: UniProtKB-KW
  2. viral release from host cell Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Host-virus interaction, Viral budding, Viral budding via the host ESCRT complexes, Virus exit from host cell

Keywords - Ligandi

Metal-binding, RNA-binding, Viral nucleoprotein, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Gag polyprotein
Alternative name(s):
Core polyprotein
Cleaved into the following 4 chains:
Gene namesi
Name:gag
OrganismiFriend murine leukemia virus (isolate PVC-211) (FrMLV)
Taxonomic identifieri11798 [NCBI]
Taxonomic lineageiVirusesRetro-transcribing virusesRetroviridaeOrthoretrovirinaeGammaretrovirusMurine leukemia virus
Virus hostiMus musculus (Mouse) [TaxID: 10090]
ProteomesiUP000007777: Genome

Subcellular locationi

Chain Gag polyprotein : Virion By similarity. Host cell membrane; Lipid-anchor Reviewed prediction. Host late endosome membrane; Lipid-anchor Reviewed prediction. Host endosomehost multivesicular body By similarity
Note: These locations are probably linked to virus assembly sites By similarity.
Chain Matrix protein p15 : Virion Reviewed prediction
Chain Capsid protein p30 : Virion Reviewed prediction

GO - Cellular componenti

  1. host cell late endosome membrane Source: UniProtKB-SubCell
  2. host cell plasma membrane Source: UniProtKB-SubCell
  3. host multivesicular body Source: UniProtKB-SubCell
  4. membrane Source: UniProtKB-KW
  5. viral nucleocapsid Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Capsid protein, Host cell membrane, Host endosome, Host membrane, Membrane, Viral matrix protein, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed; by host By similarity
Chaini2 – 538537Gag polyprotein By similarity
PRO_0000390813Add
BLAST
Chaini2 – 131130Matrix protein p15 Reviewed prediction
PRO_0000040904Add
BLAST
Chaini132 – 21584RNA-binding phosphoprotein p12 Reviewed prediction
PRO_0000040905Add
BLAST
Chaini216 – 478263Capsid protein p30 Reviewed prediction
PRO_0000040906Add
BLAST
Chaini479 – 53860Nucleocapsid protein p10 Reviewed prediction
PRO_0000040907Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi2 – 21N-myristoyl glycine; by host By similarity
Modified residuei192 – 1921Phosphoserine; by host By similarity

Post-translational modificationi

Specific enzymatic cleavages by the viral protease yield mature proteins. The protease is released by autocatalytic cleavage. The polyprotein is cleaved during and after budding, this process is termed maturation By similarity.
RNA-binding phosphoprotein p12 is phosphorylated on serine residues By similarity.

Keywords - PTMi

Lipoprotein, Myristate, Phosphoprotein

Interactioni

Subunit structurei

Capsid protein p30 is a homohexamer, that further associates as homomultimer. The virus core is composed of a lattice formed from hexagonal rings, each containing six capsid monomers By similarity.

Structurei

3D structure databases

ProteinModelPortaliP26805.
SMRiP26805. Positions 2-98, 216-346, 352-382, 479-534.

Family & Domainsi

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili438 – 47841 Reviewed prediction
Add
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi111 – 1144PTAP/PSAP motif
Motifi130 – 1345LYPX(n)L motif
Motifi162 – 1654PPXY motif

Domaini

Late-budding domains (L domains) are short sequence motifs essential for viral particle budding. They recruit proteins of the host ESCRT machinery (Endosomal Sorting Complex Required for Transport) or ESCRT-associated proteins. RNA-binding phosphoprotein p12 contains one L domain: a PPXY motif which potentially interacts with the WW domain 3 of NEDD4 E3 ubiquitin ligase. Matrix protein p15 contains one L domain: a PTAP/PSAP motif, which potentially interacts with the UEV domain of TSG101. The junction between the matrix protein p15 and RNA-binding phosphoprotein p12 also contains one L domain: a LYPX(n)L motif which potentially interacts with PDCD6IP By similarity.

Sequence similaritiesi

Keywords - Domaini

Coiled coil, Zinc-finger

Family and domain databases

Gene3Di1.10.150.180. 1 hit.
1.10.375.10. 1 hit.
4.10.60.10. 1 hit.
InterProiIPR000840. G_retro_matrix_N.
IPR002079. Gag_p12.
IPR003036. Gag_P30.
IPR008919. Retrov_capsid_N.
IPR010999. Retrovr_matrix_N.
IPR001878. Znf_CCHC.
[Graphical view]
PfamiPF01140. Gag_MA. 1 hit.
PF01141. Gag_p12. 1 hit.
PF02093. Gag_p30. 1 hit.
PF00098. zf-CCHC. 1 hit.
[Graphical view]
SMARTiSM00343. ZnF_C2HC. 1 hit.
[Graphical view]
SUPFAMiSSF47836. SSF47836. 1 hit.
SSF47943. SSF47943. 1 hit.
SSF57756. SSF57756. 1 hit.
PROSITEiPS50158. ZF_CCHC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P26805-1 [UniParc]FASTAAdd to Basket

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MGQTATTPLS LTLDHWKDVE RTAHNQSVEV RKRRWVTFCS AEWPTFNVGW    50
PRDGTFNPDI ITQVKIKVFS PGPHGHPDQV PYIVTWEALA VDPPPWVKPF 100
VHPKPPLLLP PSAPSLPPEP PLSTPPQSSL YPALTSPLNT KPRPQVLPDS 150
GGPLIDLLTE DPPPYRDPGP PSPDGKGDSG EVAPTEGAPD SSPMVSRLRG 200
RREPPVADST TSQAFPLRLG GNGQFQYWPF SSSDLYNWKN NNPSFSEDPG 250
KLTALIESVL LTHQPTWDDC QQLLGTLLTG EEKQRVLLEA RKAVRGEDGR 300
PTQLPNDIND AFPLERPDWD YNTQRGRNHL VHYRQLLLAG LQNAGRSPTN 350
LAKVKGITQG PNESPSAFLE RLKEAYRRYT PYDPEDPGQE TNVSMSFIWQ 400
SAPDIGRKLE RLEDLKNKTL GDLVREAEKI FNKRETPEER EERVRRETEE 450
KEERRRAEDE RREKERDRRR HREMSKLLAT VVSGQRQDRQ GGERRRPQLD 500
HDQCAYCKEK GHWARDCPKK PRGPRGPRPQ ASLLTLDD 538
Length:538
Mass (Da):61,033
Last modified:January 23, 2007 - v3
Checksum:i2FF9F97D2C79DEBE
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M93134 Genomic RNA. Translation: AAA46476.1.
PIRiS35474.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M93134 Genomic RNA. Translation: AAA46476.1 .
PIRi S35474.

3D structure databases

ProteinModelPortali P26805.
SMRi P26805. Positions 2-98, 216-346, 352-382, 479-534.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 1.10.150.180. 1 hit.
1.10.375.10. 1 hit.
4.10.60.10. 1 hit.
InterProi IPR000840. G_retro_matrix_N.
IPR002079. Gag_p12.
IPR003036. Gag_P30.
IPR008919. Retrov_capsid_N.
IPR010999. Retrovr_matrix_N.
IPR001878. Znf_CCHC.
[Graphical view ]
Pfami PF01140. Gag_MA. 1 hit.
PF01141. Gag_p12. 1 hit.
PF02093. Gag_p30. 1 hit.
PF00098. zf-CCHC. 1 hit.
[Graphical view ]
SMARTi SM00343. ZnF_C2HC. 1 hit.
[Graphical view ]
SUPFAMi SSF47836. SSF47836. 1 hit.
SSF47943. SSF47943. 1 hit.
SSF57756. SSF57756. 1 hit.
PROSITEi PS50158. ZF_CCHC. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Complete nucleotide sequence of a neuropathogenic variant of Friend murine leukemia virus PVC-211."
    Remington M.P., Hoffman P.M., Ruscetti S.K., Masuda M.
    Nucleic Acids Res. 20:3249-3249(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Entry informationi

Entry nameiGAG_MLVFP
AccessioniPrimary (citable) accession number: P26805
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: January 23, 2007
Last modified: February 19, 2014
This is version 99 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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