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P26798

- INO2_YEAST

UniProt

P26798 - INO2_YEAST

Protein

Protein INO2

Gene

INO2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 124 (01 Oct 2014)
      Sequence version 1 (01 Aug 1992)
      Previous versions | rss
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    Functioni

    Positive regulatory factor required for depression of the coregulated phospholipid biosynthetic enzymes. Also involved in the expression of ITR1.

    GO - Molecular functioni

    1. DNA binding Source: UniProtKB-KW
    2. protein binding Source: IntAct

    GO - Biological processi

    1. phospholipid biosynthetic process Source: SGD
    2. positive regulation of transcription from RNA polymerase II promoter Source: SGD
    3. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Activator

    Keywords - Biological processi

    Lipid biosynthesis, Lipid metabolism, Phospholipid biosynthesis, Phospholipid metabolism, Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding

    Enzyme and pathway databases

    BioCyciYEAST:G3O-29723-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protein INO2
    Gene namesi
    Name:INO2
    Synonyms:DIE1, SCS1
    Ordered Locus Names:YDR123C
    ORF Names:YD9727.18C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome IV

    Organism-specific databases

    CYGDiYDR123c.
    SGDiS000002530. INO2.

    Subcellular locationi

    Nucleus Curated

    GO - Cellular componenti

    1. nucleus Source: SGD

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 304304Protein INO2PRO_0000127253Add
    BLAST

    Proteomic databases

    PaxDbiP26798.

    Expressioni

    Gene expression databases

    GenevestigatoriP26798.

    Interactioni

    Subunit structurei

    Efficient DNA binding requires dimerization with another bHLH protein.By similarity

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    INO4P139022EBI-9262,EBI-9270

    Protein-protein interaction databases

    BioGridi32179. 162 interactions.
    DIPiDIP-680N.
    IntActiP26798. 3 interactions.
    MINTiMINT-410234.
    STRINGi4932.YDR123C.

    Structurei

    3D structure databases

    ProteinModelPortaliP26798.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini236 – 29055bHLHPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 bHLH (basic helix-loop-helix) domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG42426.
    KOiK09102.
    OrthoDBiEOG7HXD47.

    Family and domain databases

    InterProiIPR011598. bHLH_dom.
    [Graphical view]
    SMARTiSM00353. HLH. 1 hit.
    [Graphical view]
    SUPFAMiSSF47459. SSF47459. 1 hit.
    PROSITEiPS50888. BHLH. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P26798-1 [UniParc]FASTAAdd to Basket

    « Hide

    MQQATGNELL GILDLDNDID FETAYQMLSS NFDDQMSAHI HENTFSATSP    50
    PLLTHELGII PNVATVQPSH VETIPADNQT HHAPLHTHAH YLNHNPHQPS 100
    MGFDQALGLK LSPSSSGLLS TNESNAIEQF LDNLISQDMM SSNASMNSES 150
    HLHIRSPKKQ HRYTELNQRY PETHPHSNTG ELPTNTADVP TEFTTREGPH 200
    QPIGNDHYNP PPFSVPEIRI PDSDIPANIE DDPVKVRKWK HVQMEKIRRI 250
    NTKEAFERLI KSVRTPPKEN GKRIPKHILL TCVMNDIKSI RSANEALQHI 300
    LDDS 304
    Length:304
    Mass (Da):34,234
    Last modified:August 1, 1992 - v1
    Checksum:iADF0F0D745A12CE8
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X66066 Genomic DNA. Translation: CAA46866.1.
    D90460 Genomic DNA. Translation: BAA14424.1.
    Z48758 Genomic DNA. Translation: CAA88676.1.
    AY557685 Genomic DNA. Translation: AAS56011.1.
    BK006938 Genomic DNA. Translation: DAA11969.1.
    PIRiS21570.
    RefSeqiNP_010408.1. NM_001180431.1.

    Genome annotation databases

    EnsemblFungiiYDR123C; YDR123C; YDR123C.
    GeneIDi851701.
    KEGGisce:YDR123C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X66066 Genomic DNA. Translation: CAA46866.1 .
    D90460 Genomic DNA. Translation: BAA14424.1 .
    Z48758 Genomic DNA. Translation: CAA88676.1 .
    AY557685 Genomic DNA. Translation: AAS56011.1 .
    BK006938 Genomic DNA. Translation: DAA11969.1 .
    PIRi S21570.
    RefSeqi NP_010408.1. NM_001180431.1.

    3D structure databases

    ProteinModelPortali P26798.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 32179. 162 interactions.
    DIPi DIP-680N.
    IntActi P26798. 3 interactions.
    MINTi MINT-410234.
    STRINGi 4932.YDR123C.

    Proteomic databases

    PaxDbi P26798.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YDR123C ; YDR123C ; YDR123C .
    GeneIDi 851701.
    KEGGi sce:YDR123C.

    Organism-specific databases

    CYGDi YDR123c.
    SGDi S000002530. INO2.

    Phylogenomic databases

    eggNOGi NOG42426.
    KOi K09102.
    OrthoDBi EOG7HXD47.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-29723-MONOMER.

    Miscellaneous databases

    NextBioi 969374.

    Gene expression databases

    Genevestigatori P26798.

    Family and domain databases

    InterProi IPR011598. bHLH_dom.
    [Graphical view ]
    SMARTi SM00353. HLH. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47459. SSF47459. 1 hit.
    PROSITEi PS50888. BHLH. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The INO2 gene of Saccharomyces cerevisiae encodes a helix-loop-helix protein that is required for activation of phospholipid synthesis."
      Nikoloff D.M., McGraw P., Henry S.A.
      Nucleic Acids Res. 20:3253-3253(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Cloning and characterization of the SCS1 gene required for the expression of genes in yeast phospholipid synthesis."
      Hosaka K., Nikawa J., Kodaki T., Yamashita S.
      J. Biochem. 115:131-136(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
      Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T.
      , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
      Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    5. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    6. "Functional characterization of the INO2 gene of Saccharomyces cerevisiae. A positive regulator of phospholipid biosynthesis."
      Nikoloff D.M., Henry S.A.
      J. Biol. Chem. 269:7402-7411(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION.
    7. "Isolation and characterization of genes that promote the expression of inositol transporter gene ITR1 in Saccharomyces cerevisiae."
      Nikawa J., Hosaka K.
      Mol. Microbiol. 16:301-308(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION.
    8. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiINO2_YEAST
    AccessioniPrimary (citable) accession number: P26798
    Secondary accession number(s): D6VSA9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1992
    Last sequence update: August 1, 1992
    Last modified: October 1, 2014
    This is version 124 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 784 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome IV
      Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

    External Data

    Dasty 3