P26792 (INV1_DAUCA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Beta-fructofuranosidase, insoluble isoenzyme 1
EC=3.2.1.26

Alternative name(s):
Cell wall beta-fructosidase 1
Invertase 1
Sucrose hydrolase 1

Gene names

Name:

INV1

Organism

Daucus carota (Carrot)

Taxonomic identifier

4039 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › campanulids › Apiales › Apiineae › Apiaceae › Apioideae › Scandiceae › Daucinae › Daucus

Protein attributes

Sequence length	592 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May play an important role in phloem unloading and in stress response.
Catalytic activity	Hydrolysis of terminal non-reducing beta-D-fructofuranoside residues in beta-D-fructofuranosides.
Subcellular location	Secreted › cell wall. Note: Ionically bound to the cell wall.
Tissue specificity	In leaves and roots of young plants.
Induction	By wounding and bacterial infection.
Sequence similarities	Belongs to the glycosyl hydrolase 32 family.

Ontologies

Keywords
Cellular component	Cell wall Secreted
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Glycoprotein Zymogen
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological_process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular_component	cell wall Inferred from electronic annotation. Source: UniProtKB-SubCell extracellular region Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	sucrose alpha-glucosidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 39	39	Potential
	Propeptide	40 – 48	9	Potential		PRO_0000033366
	Chain	49 – 592	544	Beta-fructofuranosidase, insoluble isoenzyme 1		PRO_0000033367
Sites
	Active site	74	1	By similarity
Amino acid modifications
	Glycosylation	170	1	N-linked (GlcNAc...) (complex)		CAR_000145
	Glycosylation	195	1	N-linked (GlcNAc...) Potential
	Glycosylation	311	1	N-linked (GlcNAc...) (complex)		CAR_000146
	Glycosylation	348	1	N-linked (GlcNAc...) (high mannose)		CAR_000147
	Glycosylation	570	1	N-linked (GlcNAc...) Potential
Experimental info
	Sequence conflict	133	1	R → W in CAA49162. Ref.2
	Sequence conflict	487	1	A → V in CAA49162. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P26792 [UniParc]. Last modified August 1, 1992. Version 1. Checksum: E3DF85355D277D0C	FASTA	592	66,813
	10 20 30 40 50 60 MGVTIRNRNY DHGSLPFLQS LLAILLVTTT TLHINGVEAF HEIHYNLQSV GAENVKQVHR 70 80 90 100 110 120 TGYHFQPKQN WINDPNGPMY YKGVYHLFYQ YNPKGAVWGN IVWAHSVSTD LINWTPLEPA 130 140 150 160 170 180 IFPSKPFDKY GCRSGSATIL PGNKPVILYT GIVEGPPKNV QVQNYAIPAN LSDPYLRKWI 190 200 210 220 230 240 KPDNNPLVVA NNGENATAFR DPTTAWLDKS GHWKMLVGSK RNRRGIAYLY RSKDFIKWTK 250 260 270 280 290 300 AKHPIHSQAN TGMWECPDFF PVSLKGLNGL DTSVTGESVK HVLKVSLDLT RYEYYTVGTY 310 320 330 340 350 360 LTDKDRYIPD NTSVDGWAGL RYDYGNFYAS KTFFDPSKNR RILWGWANES DSTAHDVAKG 370 380 390 400 410 420 WAGIQLIPRT LWLDPSGKQL MQWPIEELET LRGSKVKFSR KQDLSKGILV EVKGITAAQA 430 440 450 460 470 480 DVEVTFSFKS LAKREPFDPK WLEYDAEKIC SLKGSTVQGG VGPFGLLTLA SEKLEEYTPV 490 500 510 520 530 540 FFRVFKAQNT HKVLMCSDAT RSSLKEGLYR PSFAGFVDVD LATDKKISLR SLIDNSVVES 550 560 570 580 590 FGAKGKTCIS SRVYPTLAVY ENAHLYVFNN GSETITVENL DAWSMKKPLR MN

References

[1]	"cDNA cloning of carrot extracellular beta-fructosidase and its expression in response to wounding and bacterial infection." Sturm A., Chrispeels M.J. Plant Cell 2:1107-1119(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE. Strain: cv. Queen Anne's Lace.
[2]	"Molecular characterization of the gene for carrot cell wall beta-fructosidase." Ramloch-Lorenz K., Knudsen S., Sturm A. Plant J. 4:545-554(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	M58362 mRNA. Translation: AAA03516.1. X69321 Genomic DNA. Translation: CAA49162.1.
PIR	JQ0991. S61503.
3D structure databases
ProteinModelPortal	P26792.
ModBase	Search...
Protein family/group databases
CAZy	GH32. Glycoside Hydrolase Family 32.
PTM databases
GlycoSuiteDB	P26792.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
Gene3D	2.115.10.20. 1 hit.
InterPro	IPR008985. ConA-like_lec_gl_sf. IPR001362. Glyco_hydro_32. IPR018053. Glyco_hydro_32_AS. IPR013189. Glyco_hydro_32_C. IPR013148. Glyco_hydro_32_N. IPR023296. Glyco_hydro_beta-prop. [Graphical view]
Pfam	PF08244. Glyco_hydro_32C. 1 hit. PF00251. Glyco_hydro_32N. 1 hit. [Graphical view]
SMART	SM00640. Glyco_32. 1 hit. [Graphical view]
SUPFAM	SSF49899. ConA_like_lec_gl. 1 hit. SSF75005. Glyco_hydro_43_beta-prop. 1 hit.
PROSITE	PS00609. GLYCOSYL_HYDROL_F32. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

INV1_DAUCA

Accession

Primary (citable) accession number: P26792

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1992
Last sequence update:	August 1, 1992
Last modified:	April 3, 2013
This is version 78 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program