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P26785

- RL16B_YEAST

UniProt

P26785 - RL16B_YEAST

Protein

60S ribosomal protein L16-B

Gene

RPL16B

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 128 (01 Oct 2014)
      Sequence version 3 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    GO - Molecular functioni

    1. RNA binding Source: SGD
    2. structural constituent of ribosome Source: SGD

    GO - Biological processi

    1. cytoplasmic translation Source: SGD

    Keywords - Molecular functioni

    Ribonucleoprotein, Ribosomal protein

    Enzyme and pathway databases

    BioCyciYEAST:G3O-33099-MONOMER.
    ReactomeiREACT_188965. SRP-dependent cotranslational protein targeting to membrane.
    REACT_189050. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).
    REACT_189183. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
    REACT_217188. Formation of a pool of free 40S subunits.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    60S ribosomal protein L16-B
    Alternative name(s):
    L21
    RP23
    YL15
    Gene namesi
    Name:RPL16B
    Synonyms:RP23, RPL21B
    Ordered Locus Names:YNL069C
    ORF Names:N2377
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XIV

    Organism-specific databases

    SGDiS000005013. RPL16B.

    Subcellular locationi

    Cytoplasm By similarity

    GO - Cellular componenti

    1. cytosolic large ribosomal subunit Source: SGD

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed2 Publications
    Chaini2 – 19819760S ribosomal protein L16-BPRO_0000133792Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylserine; partial2 Publications
    Modified residuei43 – 431Phosphoserine1 Publication
    Modified residuei181 – 1811Phosphoserine1 Publication
    Modified residuei185 – 1851Phosphoserine2 Publications
    Modified residuei187 – 1871Phosphoserine3 Publications

    Post-translational modificationi

    N-terminally acetylated by acetyltransferase NatA.2 Publications

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    MaxQBiP26785.
    PaxDbiP26785.
    PeptideAtlasiP26785.

    Expressioni

    Gene expression databases

    GenevestigatoriP26785.

    Interactioni

    Subunit structurei

    Component of the large ribosomal subunit. Mature ribosomes consist of a small (40S) and a large (60S) subunit. The 40S subunit contains 32 different proteins (encoded by 56 genes) and 1 molecule of RNA (18S). The 60S subunit contains 46 different proteins (encoded by 81 genes) and 3 molecules of RNA (25S, 5.8S and 5S).1 Publication

    Protein-protein interaction databases

    BioGridi35754. 135 interactions.
    IntActiP26785. 25 interactions.
    MINTiMINT-8285422.
    STRINGi4932.YNL069C.

    Structurei

    3D structure databases

    ProteinModelPortaliP26785.
    SMRiP26785. Positions 3-198.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the ribosomal protein L13P family.Curated

    Phylogenomic databases

    eggNOGiCOG0102.
    GeneTreeiENSGT00390000010799.
    HOGENOMiHOG000225289.
    KOiK02872.
    OMAiFEGIPAP.
    OrthoDBiEOG70W3RX.

    Family and domain databases

    Gene3Di3.90.1180.10. 1 hit.
    HAMAPiMF_01366. Ribosomal_L13.
    InterProiIPR005822. Ribosomal_L13.
    IPR023563. Ribosomal_L13_CS.
    IPR023564. Ribosomal_L13_dom.
    IPR005755. Ribosomal_L13_euk/arc.
    [Graphical view]
    PANTHERiPTHR11545. PTHR11545. 1 hit.
    PTHR11545:SF3. PTHR11545:SF3. 1 hit.
    PfamiPF00572. Ribosomal_L13. 1 hit.
    [Graphical view]
    PIRSFiPIRSF002181. Ribosomal_L13. 1 hit.
    SUPFAMiSSF52161. SSF52161. 1 hit.
    TIGRFAMsiTIGR01077. L13_A_E. 1 hit.
    PROSITEiPS00783. RIBOSOMAL_L13. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P26785-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSQPVVVIDA KDHLLGRLAS TIAKQVLNGQ KIVVVRAEAL NISGEFFRNK    50
    LKYHDFLRKA TAFNKTRGPF HFRAPSRILY KAIRGMVSHK TARGKAALER 100
    LKIFEGIPPP YDKKKRVVVP QALRVLRLKP GRKYTTLGKL STSVGWKYED 150
    VVAKLEDKRK VRSAEYYAKK RAFTKKVSSA SAAASESDVA KQLASFGY 198
    Length:198
    Mass (Da):22,249
    Last modified:January 23, 2007 - v3
    Checksum:i959CFEC64DAF2F83
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti14 – 141L → H AA sequence (PubMed:1544921)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X86470 Genomic DNA. Translation: CAA60191.1.
    X86470 Genomic DNA. Translation: CAA60192.1.
    Z71345 Genomic DNA. Translation: CAA95943.1.
    BK006947 Genomic DNA. Translation: DAA10476.1.
    PIRiS53911.
    RefSeqiNP_014330.1. NM_001182907.1.

    Genome annotation databases

    EnsemblFungiiYNL069C; YNL069C; YNL069C.
    GeneIDi855655.
    KEGGisce:YNL069C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X86470 Genomic DNA. Translation: CAA60191.1 .
    X86470 Genomic DNA. Translation: CAA60192.1 .
    Z71345 Genomic DNA. Translation: CAA95943.1 .
    BK006947 Genomic DNA. Translation: DAA10476.1 .
    PIRi S53911.
    RefSeqi NP_014330.1. NM_001182907.1.

    3D structure databases

    ProteinModelPortali P26785.
    SMRi P26785. Positions 3-198.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 35754. 135 interactions.
    IntActi P26785. 25 interactions.
    MINTi MINT-8285422.
    STRINGi 4932.YNL069C.

    Proteomic databases

    MaxQBi P26785.
    PaxDbi P26785.
    PeptideAtlasi P26785.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YNL069C ; YNL069C ; YNL069C .
    GeneIDi 855655.
    KEGGi sce:YNL069C.

    Organism-specific databases

    SGDi S000005013. RPL16B.

    Phylogenomic databases

    eggNOGi COG0102.
    GeneTreei ENSGT00390000010799.
    HOGENOMi HOG000225289.
    KOi K02872.
    OMAi FEGIPAP.
    OrthoDBi EOG70W3RX.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-33099-MONOMER.
    Reactomei REACT_188965. SRP-dependent cotranslational protein targeting to membrane.
    REACT_189050. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).
    REACT_189183. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
    REACT_217188. Formation of a pool of free 40S subunits.

    Miscellaneous databases

    NextBioi 979911.

    Gene expression databases

    Genevestigatori P26785.

    Family and domain databases

    Gene3Di 3.90.1180.10. 1 hit.
    HAMAPi MF_01366. Ribosomal_L13.
    InterProi IPR005822. Ribosomal_L13.
    IPR023563. Ribosomal_L13_CS.
    IPR023564. Ribosomal_L13_dom.
    IPR005755. Ribosomal_L13_euk/arc.
    [Graphical view ]
    PANTHERi PTHR11545. PTHR11545. 1 hit.
    PTHR11545:SF3. PTHR11545:SF3. 1 hit.
    Pfami PF00572. Ribosomal_L13. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF002181. Ribosomal_L13. 1 hit.
    SUPFAMi SSF52161. SSF52161. 1 hit.
    TIGRFAMsi TIGR01077. L13_A_E. 1 hit.
    PROSITEi PS00783. RIBOSOMAL_L13. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequencing a cosmid clone of Saccharomyces cerevisiae chromosome XIV reveals 12 new open reading frames (ORFs) and an ancient duplication of six ORFs."
      Poehlmann R., Philippsen P.
      Yeast 12:391-402(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 96604 / S288c / FY1679.
    2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
      Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J.
      , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
      Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. "NH2-terminal acetylation of ribosomal proteins of Saccharomyces cerevisiae."
      Takakura H., Tsunasawa S., Miyagi M., Warner J.R.
      J. Biol. Chem. 267:5442-5445(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 2-26, ACETYLATION AT SER-2 BY NATA.
    5. "The list of cytoplasmic ribosomal proteins of Saccharomyces cerevisiae."
      Planta R.J., Mager W.H.
      Yeast 14:471-477(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NOMENCLATURE, SUBUNIT.
    6. "The action of N-terminal acetyltransferases on yeast ribosomal proteins."
      Arnold R.J., Polevoda B., Reilly J.P., Sherman F.
      J. Biol. Chem. 274:37035-37040(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: CLEAVAGE OF INITIATOR METHIONINE, ACETYLATION AT SER-2 BY NATA.
    7. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
      Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
      J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-185 AND SER-187, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: ADR376.
    8. "Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
      Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
      Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-43, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    9. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-181 AND SER-187, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    10. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-185 AND SER-187, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    11. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    12. "Sites of ubiquitin attachment in Saccharomyces cerevisiae."
      Starita L.M., Lo R.S., Eng J.K., von Haller P.D., Fields S.
      Proteomics 12:236-240(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    13. "Crystal structure of the eukaryotic ribosome."
      Ben-Shem A., Jenner L., Yusupova G., Yusupov M.
      Science 330:1203-1209(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (4.0 ANGSTROMS) OF 80S RIBOSOME.
    14. Cited for: X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS) OF 80S RIBOSOME.

    Entry informationi

    Entry nameiRL16B_YEAST
    AccessioniPrimary (citable) accession number: P26785
    Secondary accession number(s): D6W1B0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1992
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 128 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    There are 2 genes for L16 in yeast.

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Ribosomal proteins
      Ribosomal proteins families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome XIV
      Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

    External Data

    Dasty 3