P26785 (RL16B_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
60S ribosomal protein L16-B

Alternative name(s):
L21
RP23
YL15

Gene names

Name:	RPL16B
Synonyms:	RP23, RPL21B
Ordered Locus Names:	YNL069C
ORF Names:	N2377

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	198 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Subunit structure	Component of the large ribosomal subunit. Mature ribosomes consist of a small (40S) and a large (60S) subunit. The 40S subunit contains 32 different proteins (encoded by 56 genes) and 1 molecule of RNA (18S). The 60S subunit contains 46 different proteins (encoded by 81 genes) and 3 molecules of RNA (25S, 5.8S and 5S). Ref.5
Subcellular location	Cytoplasm By similarity.
Post-translational modification	N-terminally acetylated by acetyltransferase NatA.
Miscellaneous	There are 2 genes for L16 in yeast.
Sequence similarities	Belongs to the ribosomal protein L13P family.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Ribonucleoprotein Ribosomal protein
PTM	Acetylation Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	cytoplasmic translation Traceable author statement Ref.5. Source: SGD
Cellular component	cytosolic large ribosomal subunit Traceable author statement Ref.5. Source: SGD
Molecular function	RNA binding Inferred from direct assay. Source: SGD structural constituent of ribosome Traceable author statement Ref.5. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.4 Ref.6
	Chain	2 – 198	197	60S ribosomal protein L16-B		PRO_0000133792
Amino acid modifications
	Modified residue	2	1	N-acetylserine; partial Ref.4 Ref.6
	Modified residue	43	1	Phosphoserine Ref.9
	Modified residue	179	1	Phosphoserine Ref.9
	Modified residue	181	1	Phosphoserine Ref.9 Ref.11
	Modified residue	185	1	Phosphoserine Ref.8 Ref.11
	Modified residue	187	1	Phosphoserine Ref.8 Ref.10 Ref.11
	Modified residue	195	1	Phosphoserine Ref.7
Experimental info
	Sequence conflict	14	1	L → H AA sequence Ref.4

Sequences

	Sequence		Length	Mass (Da)
	P26785 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 959CFEC64DAF2F83	FASTA	198	22,249
	10 20 30 40 50 60 MSQPVVVIDA KDHLLGRLAS TIAKQVLNGQ KIVVVRAEAL NISGEFFRNK LKYHDFLRKA 70 80 90 100 110 120 TAFNKTRGPF HFRAPSRILY KAIRGMVSHK TARGKAALER LKIFEGIPPP YDKKKRVVVP 130 140 150 160 170 180 QALRVLRLKP GRKYTTLGKL STSVGWKYED VVAKLEDKRK VRSAEYYAKK RAFTKKVSSA 190 SAAASESDVA KQLASFGY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequencing a cosmid clone of Saccharomyces cerevisiae chromosome XIV reveals 12 new open reading frames (ORFs) and an ancient duplication of six ORFs." Poehlmann R., Philippsen P. Yeast 12:391-402(1996) [PubMed: 8701611] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications." Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J. , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J. Nature 387:93-98(1997) [PubMed: 9169873] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"NH2-terminal acetylation of ribosomal proteins of Saccharomyces cerevisiae." Takakura H., Tsunasawa S., Miyagi M., Warner J.R. J. Biol. Chem. 267:5442-5445(1992) [PubMed: 1544921] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-26, ACETYLATION AT SER-2 BY NATA.
[5]	"The list of cytoplasmic ribosomal proteins of Saccharomyces cerevisiae." Planta R.J., Mager W.H. Yeast 14:471-477(1998) [PubMed: 9559554] [Abstract] Cited for: NOMENCLATURE, SUBUNIT.
[6]	"The action of N-terminal acetyltransferases on yeast ribosomal proteins." Arnold R.J., Polevoda B., Reilly J.P., Sherman F. J. Biol. Chem. 274:37035-37040(1999) [PubMed: 10601260] [Abstract] Cited for: CLEAVAGE OF INITIATOR METHIONINE, PARTIAL ACETYLATION AT SER-2 BY NATA.
[7]	"Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway." Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N. Mol. Cell. Proteomics 4:310-327(2005) [PubMed: 15665377] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-195, MASS SPECTROMETRY. Strain: YAL6B.
[8]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed: 17330950] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-185 AND SER-187, MASS SPECTROMETRY. Strain: ADR376.
[9]	"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry." Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F. Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed: 17287358] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-43; SER-179 AND SER-181, MASS SPECTROMETRY.
[10]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-187, MASS SPECTROMETRY.
[11]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-181; SER-185 AND SER-187, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X86470 Genomic DNA. Translation: CAA60191.1. X86470 Genomic DNA. Translation: CAA60192.1. Z71345 Genomic DNA. Translation: CAA95943.1. BK006947 Genomic DNA. Translation: DAA10476.1.
PIR	S53911.
RefSeq	NP_014330.1. NM_001182907.1.
3D structure databases
ProteinModelPortal	P26785.
SMR	P26785. Positions 3-198.
ModBase	Search...
Protein-protein interaction databases
IntAct	P26785. 21 interactions.
STRING	P26785.
Proteomic databases
PeptideAtlas	P26785.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YNL069C; YNL069C; YNL069C.
GeneID	855655.
KEGG	sce:YNL069C.
NMPDR	fig|4932.3.peg.5403.
Organism-specific databases
SGD	S000005013. RPL16B.
Phylogenomic databases
eggNOG	fuNOG04907.
GeneTree	EFGT00050000002652.
HOGENOM	HBG745735.
OMA	EFGPYFP.
OrthoDB	EOG4C2MKV.
Gene expression databases
ArrayExpress	P26785.
Genevestigator	P26785.
GermOnline	YNL069C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR005822. Ribosomal_L13. IPR023563. Ribosomal_L13_CS. IPR023564. Ribosomal_L13_dom. IPR005755. Ribosomal_L13_euk/arc. [Graphical view]
Gene3D	G3DSA:3.90.1180.10. Ribosomal_L13. 1 hit.
KO	K02872.
PANTHER	PTHR11545. Ribosomal_L13. 1 hit.
Pfam	PF00572. Ribosomal_L13. 1 hit. [Graphical view]
PIRSF	PIRSF002181. Ribosomal_L13. 1 hit.
SUPFAM	SSF52161. Ribosomal_L13. 1 hit.
TIGRFAMs	TIGR01077. L13_A_E. 1 hit.
PROSITE	PS00783. RIBOSOMAL_L13. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	979911.

Entry information

Entry name

RL16B_YEAST

Accession

Primary (citable) accession number: P26785
Secondary accession number(s): D6W1B0

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1992
Last sequence update:	January 23, 2007
Last modified:	December 14, 2011
This is version 108 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program