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P26784

- RL16A_YEAST

UniProt

P26784 - RL16A_YEAST

Protein

60S ribosomal protein L16-A

Gene

RPL16A

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 125 (01 Oct 2014)
      Sequence version 3 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    GO - Molecular functioni

    1. RNA binding Source: SGD
    2. structural constituent of ribosome Source: SGD

    GO - Biological processi

    1. cytoplasmic translation Source: SGD

    Keywords - Molecular functioni

    Ribonucleoprotein, Ribosomal protein

    Enzyme and pathway databases

    BioCyciYEAST:G3O-31384-MONOMER.
    ReactomeiREACT_188965. SRP-dependent cotranslational protein targeting to membrane.
    REACT_189050. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).
    REACT_189183. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
    REACT_217188. Formation of a pool of free 40S subunits.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    60S ribosomal protein L16-A
    Alternative name(s):
    L13a
    L21
    RP22
    YL15
    Gene namesi
    Name:RPL16A
    Synonyms:RPL13, RPL21A
    Ordered Locus Names:YIL133C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome IX

    Organism-specific databases

    SGDiS000001395. RPL16A.

    Subcellular locationi

    Cytoplasm 1 Publication

    GO - Cellular componenti

    1. cytosolic large ribosomal subunit Source: SGD

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 19919860S ribosomal protein L16-APRO_0000133791Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylserine1 Publication

    Post-translational modificationi

    N-terminally acetylated by acetyltransferase NatA.1 Publication

    Keywords - PTMi

    Acetylation

    Proteomic databases

    MaxQBiP26784.
    PeptideAtlasiP26784.

    Expressioni

    Gene expression databases

    GenevestigatoriP26784.

    Interactioni

    Subunit structurei

    Component of the large ribosomal subunit. Mature ribosomes consist of a small (40S) and a large (60S) subunit. The 40S subunit contains 32 different proteins (encoded by 56 genes) and 1 molecule of RNA (18S). The 60S subunit contains 46 different proteins (encoded by 81 genes) and 3 molecules of RNA (25S, 5.8S and 5S).1 Publication

    Protein-protein interaction databases

    BioGridi34858. 159 interactions.
    IntActiP26784. 36 interactions.
    MINTiMINT-2981627.
    STRINGi4932.YIL133C.

    Structurei

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1K5Ymodel-M2-147[»]
    1S1Ielectron microscopy11.70M2-147[»]
    1VW8electron microscopy6.10P1-199[»]
    1VWUelectron microscopy6.10P1-199[»]
    3IZSelectron microscopy-K1-199[»]
    3J65electron microscopy8.70J1-199[»]
    3O58X-ray4.00P1-199[»]
    3O5HX-ray4.00P1-199[»]
    3U5EX-ray3.00O1-199[»]
    3U5IX-ray3.00O1-199[»]
    4B6Aelectron microscopy8.10O1-199[»]
    4BYNelectron microscopy4.30O2-199[»]
    4BYUelectron microscopy6.60O2-199[»]
    4CUWelectron microscopy3.70O1-199[»]
    ProteinModelPortaliP26784.
    SMRiP26784. Positions 4-199.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP26784.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the ribosomal protein L13P family.Curated

    Phylogenomic databases

    GeneTreeiENSGT00390000010799.
    HOGENOMiHOG000225289.
    KOiK02872.
    OMAiKTHYNPE.
    OrthoDBiEOG70W3RX.

    Family and domain databases

    Gene3Di3.90.1180.10. 1 hit.
    HAMAPiMF_01366. Ribosomal_L13.
    InterProiIPR005822. Ribosomal_L13.
    IPR023563. Ribosomal_L13_CS.
    IPR023564. Ribosomal_L13_dom.
    IPR005755. Ribosomal_L13_euk/arc.
    [Graphical view]
    PANTHERiPTHR11545. PTHR11545. 1 hit.
    PTHR11545:SF3. PTHR11545:SF3. 1 hit.
    PfamiPF00572. Ribosomal_L13. 1 hit.
    [Graphical view]
    PIRSFiPIRSF002181. Ribosomal_L13. 1 hit.
    SUPFAMiSSF52161. SSF52161. 1 hit.
    TIGRFAMsiTIGR01077. L13_A_E. 1 hit.
    PROSITEiPS00783. RIBOSOMAL_L13. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P26784-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSVEPVVVID GKGHLVGRLA SVVAKQLLNG QKIVVVRAEE LNISGEFFRN    50
    KLKYHDFLRK ATAFNKTRGP FHFRAPSRIF YKALRGMVSH KTARGKAALE 100
    RLKVFEGIPP PYDKKKRVVV PQALRVLRLK PGRKYTTLGK LSTSVGWKYE 150
    DVVAKLEAKR KVSSAEYYAK KRAFTKKVAS ANATAAESDV AKQLAALGY 199
    Length:199
    Mass (Da):22,201
    Last modified:January 23, 2007 - v3
    Checksum:iDAB11B0D4A91EF0B
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti14 – 141H → L AA sequence (PubMed:1544921)Curated
    Sequence conflicti21 – 211S → E AA sequence (PubMed:1544921)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z38059 Genomic DNA. Translation: CAA86145.1.
    BK006942 Genomic DNA. Translation: DAA08420.1.
    PIRiS48401.
    RefSeqiNP_012133.1. NM_001179481.1.

    Genome annotation databases

    EnsemblFungiiYIL133C; YIL133C; YIL133C.
    GeneIDi854673.
    KEGGisce:YIL133C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z38059 Genomic DNA. Translation: CAA86145.1 .
    BK006942 Genomic DNA. Translation: DAA08420.1 .
    PIRi S48401.
    RefSeqi NP_012133.1. NM_001179481.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1K5Y model - M 2-147 [» ]
    1S1I electron microscopy 11.70 M 2-147 [» ]
    1VW8 electron microscopy 6.10 P 1-199 [» ]
    1VWU electron microscopy 6.10 P 1-199 [» ]
    3IZS electron microscopy - K 1-199 [» ]
    3J65 electron microscopy 8.70 J 1-199 [» ]
    3O58 X-ray 4.00 P 1-199 [» ]
    3O5H X-ray 4.00 P 1-199 [» ]
    3U5E X-ray 3.00 O 1-199 [» ]
    3U5I X-ray 3.00 O 1-199 [» ]
    4B6A electron microscopy 8.10 O 1-199 [» ]
    4BYN electron microscopy 4.30 O 2-199 [» ]
    4BYU electron microscopy 6.60 O 2-199 [» ]
    4CUW electron microscopy 3.70 O 1-199 [» ]
    ProteinModelPortali P26784.
    SMRi P26784. Positions 4-199.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 34858. 159 interactions.
    IntActi P26784. 36 interactions.
    MINTi MINT-2981627.
    STRINGi 4932.YIL133C.

    Proteomic databases

    MaxQBi P26784.
    PeptideAtlasi P26784.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YIL133C ; YIL133C ; YIL133C .
    GeneIDi 854673.
    KEGGi sce:YIL133C.

    Organism-specific databases

    SGDi S000001395. RPL16A.

    Phylogenomic databases

    GeneTreei ENSGT00390000010799.
    HOGENOMi HOG000225289.
    KOi K02872.
    OMAi KTHYNPE.
    OrthoDBi EOG70W3RX.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-31384-MONOMER.
    Reactomei REACT_188965. SRP-dependent cotranslational protein targeting to membrane.
    REACT_189050. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).
    REACT_189183. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
    REACT_217188. Formation of a pool of free 40S subunits.

    Miscellaneous databases

    EvolutionaryTracei P26784.
    NextBioi 977265.

    Gene expression databases

    Genevestigatori P26784.

    Family and domain databases

    Gene3Di 3.90.1180.10. 1 hit.
    HAMAPi MF_01366. Ribosomal_L13.
    InterProi IPR005822. Ribosomal_L13.
    IPR023563. Ribosomal_L13_CS.
    IPR023564. Ribosomal_L13_dom.
    IPR005755. Ribosomal_L13_euk/arc.
    [Graphical view ]
    PANTHERi PTHR11545. PTHR11545. 1 hit.
    PTHR11545:SF3. PTHR11545:SF3. 1 hit.
    Pfami PF00572. Ribosomal_L13. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF002181. Ribosomal_L13. 1 hit.
    SUPFAMi SSF52161. SSF52161. 1 hit.
    TIGRFAMsi TIGR01077. L13_A_E. 1 hit.
    PROSITEi PS00783. RIBOSOMAL_L13. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    3. "NH2-terminal acetylation of ribosomal proteins of Saccharomyces cerevisiae."
      Takakura H., Tsunasawa S., Miyagi M., Warner J.R.
      J. Biol. Chem. 267:5442-5445(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 2-26, ACETYLATION AT SER-2 BY NATA.
    4. "The list of cytoplasmic ribosomal proteins of Saccharomyces cerevisiae."
      Planta R.J., Mager W.H.
      Yeast 14:471-477(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NOMENCLATURE, SUBUNIT.
    5. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    7. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    8. "Sites of ubiquitin attachment in Saccharomyces cerevisiae."
      Starita L.M., Lo R.S., Eng J.K., von Haller P.D., Fields S.
      Proteomics 12:236-240(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    9. "Structure of the 80S ribosome from Saccharomyces cerevisiae -- tRNA-ribosome and subunit-subunit interactions."
      Spahn C.M.T., Beckmann R., Eswar N., Penczek P.A., Sali A., Blobel G., Frank J.
      Cell 107:373-386(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: 3D-STRUCTURE MODELING OF 2-147, ELECTRON MICROSCOPY.
    10. "Domain movements of elongation factor eEF2 and the eukaryotic 80S ribosome facilitate tRNA translocation."
      Spahn C.M.T., Gomez-Lorenzo M.G., Grassucci R.A., Joergensen R., Andersen G.R., Beckmann R., Penczek P.A., Ballesta J.P.G., Frank J.
      EMBO J. 23:1008-1019(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: 3D-STRUCTURE MODELING OF 2-147, ELECTRON MICROSCOPY.
    11. "Crystal structure of the eukaryotic ribosome."
      Ben-Shem A., Jenner L., Yusupova G., Yusupov M.
      Science 330:1203-1209(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (4.0 ANGSTROMS) OF 80S RIBOSOME.
    12. Cited for: X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS) OF 80S RIBOSOME.

    Entry informationi

    Entry nameiRL16A_YEAST
    AccessioniPrimary (citable) accession number: P26784
    Secondary accession number(s): D6VVF4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1992
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 125 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 43300 molecules/cell in log phase SD medium.1 Publication
    There are 2 genes for L16 in yeast.

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. Ribosomal proteins
      Ribosomal proteins families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families
    4. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    5. Yeast chromosome IX
      Yeast (Saccharomyces cerevisiae) chromosome IX: entries and gene names

    External Data

    Dasty 3