60S ribosomal protein L16-A - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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P26784 (RL16A_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 29, 2013. Version 113. History...

Clusters with 100%, 90%, 50% identity |

Documents (5) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
60S ribosomal protein L16-A

Alternative name(s):
L13a
L21
RP22
YL15

Gene names

Name:	RPL16A
Synonyms:	RPL13, RPL21A
Ordered Locus Names:	YIL133C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	199 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Subunit structure	Component of the large ribosomal subunit. Mature ribosomes consist of a small (40S) and a large (60S) subunit. The 40S subunit contains 32 different proteins (encoded by 56 genes) and 1 molecule of RNA (18S). The 60S subunit contains 46 different proteins (encoded by 81 genes) and 3 molecules of RNA (25S, 5.8S and 5S). Ref.4
Subcellular location	Cytoplasm Ref.5.
Post-translational modification	N-terminally acetylated by acetyltransferase NatA.
Miscellaneous	Present with 43300 molecules/cell in log phase SD medium. There are 2 genes for L16 in yeast.
Sequence similarities	Belongs to the ribosomal protein L13P family.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Ribonucleoprotein Ribosomal protein
PTM	Acetylation
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	cytoplasmic translation Traceable author statement Ref.4. Source: SGD
Cellular_component	cytosolic large ribosomal subunit Traceable author statement Ref.4. Source: SGD
Molecular_function	RNA binding Inferred from direct assay PubMed 6337137. Source: SGD structural constituent of ribosome Traceable author statement Ref.4. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

Removed Ref.3

Chain

2 – 199

198

60S ribosomal protein L16-A

PRO_0000133791

Amino acid modifications

Modified residue

N-acetylserine Ref.3

Experimental info

Sequence conflict

H → L AA sequence Ref.3

Sequence conflict

S → E AA sequence Ref.3

Sequences

Sequence

Length

Mass (Da)

Tools

P26784 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: DAB11B0D4A91EF0B
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FASTA

199

22,201

        10         20         30         40         50         60 
MSVEPVVVID GKGHLVGRLA SVVAKQLLNG QKIVVVRAEE LNISGEFFRN KLKYHDFLRK 

        70         80         90        100        110        120 
ATAFNKTRGP FHFRAPSRIF YKALRGMVSH KTARGKAALE RLKVFEGIPP PYDKKKRVVV 

       130        140        150        160        170        180 
PQALRVLRLK PGRKYTTLGK LSTSVGWKYE DVVAKLEAKR KVSSAEYYAK KRAFTKKVAS 

       190 
ANATAAESDV AKQLAALGY

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome IX." Churcher C.M., Bowman S., Badcock K., Bankier A.T., Brown D., Chillingworth T., Connor R., Devlin K., Gentles S., Hamlin N., Harris D.E., Horsnell T., Hunt S., Jagels K., Jones M., Lye G., Moule S., Odell C. Barrell B.G. Nature 387:84-87(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"NH2-terminal acetylation of ribosomal proteins of Saccharomyces cerevisiae." Takakura H., Tsunasawa S., Miyagi M., Warner J.R. J. Biol. Chem. 267:5442-5445(1992) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-26, ACETYLATION AT SER-2 BY NATA.
[4]	"The list of cytoplasmic ribosomal proteins of Saccharomyces cerevisiae." Planta R.J., Mager W.H. Yeast 14:471-477(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NOMENCLATURE, SUBUNIT.
[5]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]	"Structure of the 80S ribosome from Saccharomyces cerevisiae -- tRNA-ribosome and subunit-subunit interactions." Spahn C.M.T., Beckmann R., Eswar N., Penczek P.A., Sali A., Blobel G., Frank J. Cell 107:373-386(2001) [PubMed] [Europe PMC] [Abstract] Cited for: 3D-STRUCTURE MODELING OF 2-147, ELECTRON MICROSCOPY.
[8]	"Domain movements of elongation factor eEF2 and the eukaryotic 80S ribosome facilitate tRNA translocation." Spahn C.M.T., Gomez-Lorenzo M.G., Grassucci R.A., Joergensen R., Andersen G.R., Beckmann R., Penczek P.A., Ballesta J.P.G., Frank J. EMBO J. 23:1008-1019(2004) [PubMed] [Europe PMC] [Abstract] Cited for: 3D-STRUCTURE MODELING OF 2-147, ELECTRON MICROSCOPY.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

Z38059 Genomic DNA. Translation: CAA86145.1.
BK006942 Genomic DNA. Translation: DAA08420.1.

PIR

S48401.

RefSeq

NP_012133.1. NM_001179481.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1K5Y	model	-	M	2-147	[»]
1S1I	electron microscopy	11.70	M	2-147	[»]
3IZS	electron microscopy	-	K	1-199	[»]
3O58	X-ray	4.00	P	1-199	[»]
3O5H	X-ray	4.00	P	1-199	[»]
3U5E	X-ray	3.00	O	1-199	[»]
3U5I	X-ray	3.00	O	1-199	[»]
4B6A	electron microscopy	8.10	O	5-199	[»]

ProteinModelPortal

P26784.

SMR

P26784. Positions 4-199.

ModBase

Search...

Protein-protein interaction databases

IntAct

P26784. 30 interactions.

MINT

MINT-2981627.

STRING

4932.YIL133C.

Proteomic databases

PeptideAtlas

P26784.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblFungi

YIL133C; YIL133C; YIL133C.

GeneID

854673.

KEGG

sce:YIL133C.

Organism-specific databases

SGD

S000001395. RPL16A.

Phylogenomic databases

GeneTree

ENSGT00390000010799.

HOGENOM

HOG000225289.

K02872.

OMA

EFGPYFP.

OrthoDB

EOG4C2MKV.

Enzyme and pathway databases

BioCyc

YEAST:G3O-31384-MONOMER.

Gene expression databases

ArrayExpress

P26784.

Genevestigator

P26784.

GermOnline

YIL133C. Saccharomyces cerevisiae.

Family and domain databases

Gene3D

3.90.1180.10. 1 hit.

InterPro

IPR005822. Ribosomal_L13.
IPR023563. Ribosomal_L13_CS.
IPR023564. Ribosomal_L13_dom.
IPR005755. Ribosomal_L13_euk/arc.
[Graphical view]

PANTHER

PTHR11545. PTHR11545. 1 hit.
PTHR11545:SF3. PTHR11545:SF3. 1 hit.

Pfam

PF00572. Ribosomal_L13. 1 hit.
[Graphical view]

PIRSF

PIRSF002181. Ribosomal_L13. 1 hit.

SUPFAM

SSF52161. Ribosomal_L13. 1 hit.

TIGRFAMs

TIGR01077. L13_A_E. 1 hit.

PROSITE

PS00783. RIBOSOMAL_L13. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P26784.

NextBio

977265.

Entry information

Entry name

RL16A_YEAST

Accession

Primary (citable) accession number: P26784
Secondary accession number(s): D6VVF4

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1992
Last sequence update:	January 23, 2007
Last modified:	May 29, 2013
This is version 113 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome IX

Yeast (Saccharomyces cerevisiae) chromosome IX: entries and gene names

Ribosomal proteins

Ribosomal proteins families and list of entries

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents