40S ribosomal protein S5 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Contribute

Send feedback

Read comments (?) or add your own

P26783 (RS5_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 29, 2013. Version 121. History...

Clusters with 100%, 90%, 50% identity |

Documents (5) |

Third-party data

text xml rdf/xml gff fasta

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
40S ribosomal protein S5

Alternative name(s):
RP14
S2
YS8

Gene names

Name:	RPS5
Synonyms:	RPS2
Ordered Locus Names:	YJR123W
ORF Names:	J2045

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	225 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Subunit structure	Component of the small ribosomal subunit. Mature ribosomes consist of a small (40S) and a large (60S) subunit. The 40S subunit contains 32 different proteins (encoded by 56 genes) and 1 molecule of RNA (18S). The 60S subunit contains 46 different proteins (encoded by 81 genes) and 3 molecules of RNA (25S, 5.8S and 5S). Ref.6
Subcellular location	Cytoplasm By similarity.
Post-translational modification	N-terminally acetylated by acetyltransferase NatA.
Sequence similarities	Belongs to the ribosomal protein S7P family.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Ribonucleoprotein Ribosomal protein
PTM	Acetylation Phosphoprotein
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	cytoplasmic translation Inferred by curator PubMed 385049 PubMed 6814480. Source: SGD rRNA export from nucleus Inferred from mutant phenotype PubMed 16246728. Source: SGD regulation of translational fidelity Inferred from sequence alignment PubMed 17901157. Source: SGD
Cellular_component	90S preribosome Inferred from direct assay PubMed 12150911. Source: SGD cytosolic small ribosomal subunit Inferred from direct assay PubMed 385049 PubMed 6814480. Source: SGD
Molecular_function	RNA binding Inferred from electronic annotation. Source: InterPro structural constituent of ribosome Inferred from direct assay PubMed 6814480. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
BUL1	P48524	3	EBI-16150,EBI-3881

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

Removed Ref.5 Ref.7

Chain

2 – 225

224

40S ribosomal protein S5

PRO_0000124540

Amino acid modifications

Modified residue

N-acetylserine Ref.5 Ref.7

Modified residue

Phosphothreonine Ref.8

Experimental info

Sequence conflict

T → E AA sequence Ref.5

Secondary structure

225

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

P26783 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: CD3F743B56CB1127
Show »

FASTA

225

25,039

        10         20         30         40         50         60 
MSDTEAPVEV QEDFEVVEEF TPVVLATPIP EEVQQAQTEI KLFNKWSFEE VEVKDASLVD 

        70         80         90        100        110        120 
YVQVRQPIFV AHTAGRYANK RFRKAQCPII ERLTNSLMMN GRNNGKKLKA VRIIKHTLDI 

       130        140        150        160        170        180 
INVLTDQNPI QVVVDAITNT GPREDTTRVG GGGAARRQAV DVSPLRRVNQ AIALLTIGAR 

       190        200        210        220 
EAAFRNIKTI AETLAEELIN AAKGSSTSYA IKKKDELERV AKSNR

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and characterisation of the gene encoding the ribosomal protein S5 (also known as rp14, S2, YS8) of Saccharomyces cerevisiae." Ignatovich O., Cooper M., Kulesza H.M., Beggs J.D. Nucleic Acids Res. 23:4616-4619(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X." Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K. Karpfinger-Hartl L. EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. LaBaer J. Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[5]	"NH2-terminal acetylation of ribosomal proteins of Saccharomyces cerevisiae." Takakura H., Tsunasawa S., Miyagi M., Warner J.R. J. Biol. Chem. 267:5442-5445(1992) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-21, ACETYLATION AT SER-2 BY NATA.
[6]	"The list of cytoplasmic ribosomal proteins of Saccharomyces cerevisiae." Planta R.J., Mager W.H. Yeast 14:471-477(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NOMENCLATURE, SUBUNIT.
[7]	"The action of N-terminal acetyltransferases on yeast ribosomal proteins." Arnold R.J., Polevoda B., Reilly J.P., Sherman F. J. Biol. Chem. 274:37035-37040(1999) [PubMed] [Europe PMC] [Abstract] Cited for: CLEAVAGE OF INITIATOR METHIONINE, ACETYLATION AT SER-2 BY NATA.
[8]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-27, MASS SPECTROMETRY.
[9]	"Structure of the 80S ribosome from Saccharomyces cerevisiae -- tRNA-ribosome and subunit-subunit interactions." Spahn C.M.T., Beckmann R., Eswar N., Penczek P.A., Sali A., Blobel G., Frank J. Cell 107:373-386(2001) [PubMed] [Europe PMC] [Abstract] Cited for: 3D-STRUCTURE MODELING OF 76-225, ELECTRON MICROSCOPY.
[10]	"Domain movements of elongation factor eEF2 and the eukaryotic 80S ribosome facilitate tRNA translocation." Spahn C.M.T., Gomez-Lorenzo M.G., Grassucci R.A., Joergensen R., Andersen G.R., Beckmann R., Penczek P.A., Ballesta J.P.G., Frank J. EMBO J. 23:1008-1019(2004) [PubMed] [Europe PMC] [Abstract] Cited for: 3D-STRUCTURE MODELING OF 76-225, ELECTRON MICROSCOPY.
[11]	"Structure of the ribosome-bound cricket paralysis virus IRES RNA." Schueler M., Connell S.R., Lescoute A., Giesebrecht J., Dabrowski M., Schroeer B., Mielke T., Penczek P.A., Westhof E., Spahn C.M.T. Nat. Struct. Mol. Biol. 13:1092-1096(2006) [PubMed] [Europe PMC] [Abstract] Cited for: STRUCTURE BY ELECTRON MICROSCOPY (7.3 ANGSTROMS) OF 76-225.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X89368 Genomic DNA. Translation: CAA61550.1.
Z49623 Genomic DNA. Translation: CAA89654.1.
AY692868 Genomic DNA. Translation: AAT92887.1.
BK006943 Genomic DNA. Translation: DAA08908.1.

PIR

S55720.

RefSeq

NP_012657.1. NM_001181781.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1K5X	model	-	G	76-225	[»]
1S1H	electron microscopy	11.70	G	76-225	[»]
2NOQ	electron microscopy	7.30	F	76-225	[»]
3IZB	electron microscopy	-	F	1-225	[»]
3O2Z	X-ray	4.00	D	1-225	[»]
3O30	X-ray	4.00	D	1-225	[»]
3U5C	X-ray	3.00	F	1-225	[»]
3U5G	X-ray	3.00	F	1-225	[»]

ProteinModelPortal

P26783.

SMR

P26783. Positions 20-225.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-5092N.

IntAct

P26783. 61 interactions.

MINT

MINT-480795.

STRING

4932.YJR123W.

2D gel databases

UCD-2DPAGE

P26783.

Proteomic databases

PaxDb

P26783.

PeptideAtlas

P26783.

PRIDE

P26783.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblFungi

YJR123W; YJR123W; YJR123W.

GeneID

853587.

KEGG

sce:YJR123W.

Organism-specific databases

SGD

S000003884. RPS5.

Phylogenomic databases

eggNOG

COG0049.

GeneTree

ENSGT00390000010806.

HOGENOM

HOG000039066.

K02989.

OMA

PREETTR.

OrthoDB

EOG4TF3VJ.

Enzyme and pathway databases

BioCyc

YEAST:G3O-31744-MONOMER.

Gene expression databases

Genevestigator

P26783.

GermOnline

YJR123W. Saccharomyces cerevisiae.

Family and domain databases

Gene3D

1.10.455.10. 1 hit.

InterPro

IPR000235. Ribosomal_S5/S7.
IPR005716. Ribosomal_S5/S7_euk/arc.
IPR020606. Ribosomal_S7_CS.
IPR023798. Ribosomal_S7_dom.
[Graphical view]

PANTHER

PTHR11205. PTHR11205. 1 hit.
PTHR11205:SF1. PTHR11205:SF1. 1 hit.

Pfam

PF00177. Ribosomal_S7. 1 hit.
[Graphical view]

SUPFAM

SSF47973. Ribosomal_S7. 1 hit.

TIGRFAMs

TIGR01028. S7_S5_E_A. 1 hit.

PROSITE

PS00052. RIBOSOMAL_S7. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P26783.

NextBio

974388.

Entry information

Entry name

RS5_YEAST

Accession

Primary (citable) accession number: P26783
Secondary accession number(s): D6VWU2

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1992
Last sequence update:	January 23, 2007
Last modified:	May 29, 2013
This is version 121 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome X

Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

Ribosomal proteins

Ribosomal proteins families and list of entries

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Amino acid modifications

Experimental info

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

2D gel databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents