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Protein

Adenylate cyclase type 4

Gene

Adcy4

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

This is a membrane-bound, calmodulin-insensitive adenylyl cyclase.

Catalytic activityi

ATP = 3',5'-cyclic AMP + diphosphate.

Cofactori

Mg2+By similarityNote: Binds 2 magnesium ions per subunit.By similarity

Enzyme regulationi

Insensitive to calcium/calmodulin. Stimulated by the G protein beta and gamma subunit complex.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi278 – 2781Magnesium 1PROSITE-ProRule annotation
Metal bindingi278 – 2781Magnesium 2PROSITE-ProRule annotation
Metal bindingi279 – 2791Magnesium 2; via carbonyl oxygenPROSITE-ProRule annotation
Metal bindingi322 – 3221Magnesium 1PROSITE-ProRule annotation
Metal bindingi322 – 3221Magnesium 2PROSITE-ProRule annotation

GO - Molecular functioni

  • adenylate cyclase activity Source: BHF-UCL
  • ATP binding Source: UniProtKB-KW
  • G-protein beta/gamma-subunit complex binding Source: RGD
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • cAMP biosynthetic process Source: BHF-UCL
  • intracellular signal transduction Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

cAMP biosynthesis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Adenylate cyclase type 4 (EC:4.6.1.1)
Alternative name(s):
ATP pyrophosphate-lyase 4
Adenylate cyclase type IV
Adenylyl cyclase 4
Gene namesi
Name:Adcy4
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494 Componenti: Unplaced

Organism-specific databases

RGDi2034. Adcy4.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 2828CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei29 – 5022HelicalSequence AnalysisAdd
BLAST
Transmembranei61 – 8020HelicalSequence AnalysisAdd
BLAST
Transmembranei94 – 11724HelicalSequence AnalysisAdd
BLAST
Transmembranei120 – 13819HelicalSequence AnalysisAdd
BLAST
Transmembranei141 – 16222HelicalSequence AnalysisAdd
BLAST
Transmembranei170 – 19021HelicalSequence AnalysisAdd
BLAST
Topological domaini191 – 582392CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei583 – 60422HelicalSequence AnalysisAdd
BLAST
Transmembranei608 – 63023HelicalSequence AnalysisAdd
BLAST
Transmembranei661 – 68424HelicalSequence AnalysisAdd
BLAST
Topological domaini685 – 70723ExtracellularSequence AnalysisAdd
BLAST
Transmembranei708 – 73326HelicalSequence AnalysisAdd
BLAST
Transmembranei741 – 76121HelicalSequence AnalysisAdd
BLAST
Transmembranei788 – 80417HelicalSequence AnalysisAdd
BLAST
Topological domaini805 – 1064260CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • intracellular Source: GOC
  • membrane Source: BHF-UCL
  • plasma membrane Source: InterPro
  • protein complex Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10641064Adenylate cyclase type 4PRO_0000195692Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi694 – 6941N-linked (GlcNAc...)Sequence Analysis
Glycosylationi701 – 7011N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiP26770.
PRIDEiP26770.

PTM databases

PhosphoSiteiP26770.

Expressioni

Tissue specificityi

Widely distributed.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000027719.

Structurei

3D structure databases

ProteinModelPortaliP26770.
SMRiP26770. Positions 261-447, 854-1053.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the adenylyl cyclase class-4/guanylyl cyclase family.PROSITE-ProRule annotation
Contains 2 guanylate cyclase domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG2114.
HOGENOMiHOG000006941.
HOVERGENiHBG050458.
InParanoidiP26770.

Family and domain databases

Gene3Di3.30.70.1230. 2 hits.
InterProiIPR001054. A/G_cyclase.
IPR018297. A/G_cyclase_CS.
IPR030672. Adcy.
IPR009398. Adcy_conserved_dom.
IPR029787. Nucleotide_cyclase.
[Graphical view]
PfamiPF06327. DUF1053. 1 hit.
PF00211. Guanylate_cyc. 2 hits.
[Graphical view]
PIRSFiPIRSF039050. Ade_cyc. 1 hit.
SMARTiSM00044. CYCc. 2 hits.
[Graphical view]
SUPFAMiSSF55073. SSF55073. 2 hits.
PROSITEiPS00452. GUANYLATE_CYCLASE_1. 2 hits.
PS50125. GUANYLATE_CYCLASE_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P26770-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MARLFSPRPP PSEDLFYETY YSLSQQYPLL ILLLVIVLCA IVALPAVAWA
60 70 80 90 100
SGRELTSDPS FLTTVLCALG GFSLLLGLAS REQQLQRWTR PLSGLIWAAL
110 120 130 140 150
LALGYGFLFT GGVVSAWDQV SFFLFIIFTV YAMLPLGMRD AAAAGVISSL
160 170 180 190 200
SHLLVLGLYL GWRPESQRDL LPQLAANAVL FLCGNVVGAY HKALMERALR
210 220 230 240 250
ATFREALSSL HSRRRLDTEK KHQEHLLLSI LPAYLAREMK AEIMARLQAG
260 270 280 290 300
QSSRPENTNN FHSLYVKRHQ GVSVLYADIV GFTRLASECS PKELVLMLNE
310 320 330 340 350
LFGKFDQIAK EHECMRIKIL GDCYYCVSGL PLSLPDHAIN CVRMGLDMCR
360 370 380 390 400
AIRKLRVATG VDINMRVGVH SGSVLCGVIG LQKWQYDVWS HDVTLANHME
410 420 430 440 450
AGGVPGRVHI TGATLALLAG AYAVERADME HRDPYLRELG EPTYLVIDPW
460 470 480 490 500
AEEEDEKGTE RGLLSSLEGH TMRPSLLMTR YLESWGAAKP FAHLSHVDSP
510 520 530 540 550
ASTSTPLPEK AFSPQWSLDR SRTPRGLHDE LDTGDAKFFQ VIEQLNSQKQ
560 570 580 590 600
WKQSKDFNLL TLYFREKEME KQYRLSALPA FKYYAACTFL VFLSNFTIQM
610 620 630 640 650
LVTTRPPALA TTYSITFLLF LLLLFVCFSE HLTKCVQKGP KMLHWLPALS
660 670 680 690 700
VLVATRPGLR VALGTATILL VFTMAVVSLL FLPVSSDCPF LAPNVSSVAF
710 720 730 740 750
NTSWELPASL PLISIPYSMH CCVLGFLSCS LFLHMSFELK LLLLLLWLVA
760 770 780 790 800
SCSLFLHSHA WLSDCLIARL YQGSLGSRPG VLKEPKLMGA IYFFIFFFTL
810 820 830 840 850
LVLARQNEYY CRLDFLWKKK LRQEREETET MENVLPAHVA PQLIGQNRRN
860 870 880 890 900
EDLYHQSYEC VCVLFASIPD FKEFYSESNI NHEGLECLRL LNEIIADFDE
910 920 930 940 950
LLSKPKFSGV EKIKTIGSTY MAATGLNATP GQDTQQDAER SCSHLGTMVE
960 970 980 990 1000
FAVALGSKLG VINKHSFNNF RLRVGLNHGP VVAGVIGAQK PQYDIWGNTV
1010 1020 1030 1040 1050
NVASRMESTG VLGKIQVTEE TARALQSLGY TCYSRGVIKV KGKGQLCTYF
1060
LNTDLTRTGS PSAS
Length:1,064
Mass (Da):118,799
Last modified:August 1, 1992 - v1
Checksum:i5A2A0B895B5A0DA8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M80633 mRNA. Translation: AAA40665.1.
PIRiA41542.
UniGeneiRn.1904.

Genome annotation databases

UCSCiRGD:2034. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M80633 mRNA. Translation: AAA40665.1.
PIRiA41542.
UniGeneiRn.1904.

3D structure databases

ProteinModelPortaliP26770.
SMRiP26770. Positions 261-447, 854-1053.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000027719.

Chemistry

ChEMBLiCHEMBL2095179.

PTM databases

PhosphoSiteiP26770.

Proteomic databases

PaxDbiP26770.
PRIDEiP26770.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

UCSCiRGD:2034. rat.

Organism-specific databases

RGDi2034. Adcy4.

Phylogenomic databases

eggNOGiCOG2114.
HOGENOMiHOG000006941.
HOVERGENiHBG050458.
InParanoidiP26770.

Miscellaneous databases

PROiP26770.

Family and domain databases

Gene3Di3.30.70.1230. 2 hits.
InterProiIPR001054. A/G_cyclase.
IPR018297. A/G_cyclase_CS.
IPR030672. Adcy.
IPR009398. Adcy_conserved_dom.
IPR029787. Nucleotide_cyclase.
[Graphical view]
PfamiPF06327. DUF1053. 1 hit.
PF00211. Guanylate_cyc. 2 hits.
[Graphical view]
PIRSFiPIRSF039050. Ade_cyc. 1 hit.
SMARTiSM00044. CYCc. 2 hits.
[Graphical view]
SUPFAMiSSF55073. SSF55073. 2 hits.
PROSITEiPS00452. GUANYLATE_CYCLASE_1. 2 hits.
PS50125. GUANYLATE_CYCLASE_2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Cloning and expression of a widely distributed (type IV) adenylyl cyclase."
    Gao B., Gilman A.G.
    Proc. Natl. Acad. Sci. U.S.A. 88:10178-10182(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Testis.

Entry informationi

Entry nameiADCY4_RAT
AccessioniPrimary (citable) accession number: P26770
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: August 1, 1992
Last modified: June 24, 2015
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.