P26755 (RFA3_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Replication factor A protein 3
Short name=RF-A

Alternative name(s):
Replication protein A 13 kDa subunit

Gene names

Name:	RFA3
Ordered Locus Names:	YJL173C
ORF Names:	J0506

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	122 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Binds single-stranded DNA. Stimulates the activity of a cognate strand exchange protein (SEP1). It cooperates with T-AG and DNA topoisomerase I to unwind template DNA containing the simian virus 40 origin of DNA replication.
Subunit structure	Heterotrimer of 69, 36, and 13 kDa chains. The DNA-binding activity may reside exclusively on the 69 kDa subunit.
Subcellular location	Nucleus.
Post-translational modification	The N-terminus is blocked.
Miscellaneous	Present with 4280 molecules/cell in log phase SD medium. Ref.6

Ontologies

Keywords
Biological process	DNA replication
Cellular component	Nucleus
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	DNA unwinding involved in replication Inferred from direct assay. Source: SGD double-strand break repair via homologous recombination Inferred from genetic interaction. Source: SGD establishment of protein localization Inferred from physical interaction. Source: SGD heteroduplex formation Inferred from direct assay. Source: SGD nucleotide-excision repair Inferred from direct assay. Source: SGD protein ubiquitination Inferred from physical interaction. Source: SGD reciprocal meiotic recombination Inferred from physical interaction. Source: SGD telomere maintenance via recombination Inferred from physical interaction. Source: SGD telomere maintenance via telomerase Inferred from physical interaction. Source: SGD
Cellular component	DNA replication factor A complex Inferred from direct assay. Source: SGD chromosome, telomeric region Inferred from mutant phenotype. Source: SGD condensed nuclear chromosome Inferred from direct assay. Source: SGD
Molecular function	double-stranded DNA binding Inferred from direct assay. Source: SGD sequence-specific DNA binding Inferred from direct assay. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 122	122	Replication factor A protein 3		PRO_0000097279

Sequences

	Sequence		Length	Mass (Da)
	P26755 [UniParc]. Last modified August 1, 1992. Version 1. Checksum: 5EC5D6FB56BAC008	FASTA	122	13,816
	10 20 30 40 50 60 MASETPRVDP TEISNVNAPV FRIIAQIKSQ PTESQLILQS PTISSKNGSE VEMITLNNIR 70 80 90 100 110 120 VSMNKTFEID SWYEFVCRNN DDGELGFLIL DAVLCKFKEN EDLSLNGVVA LQRLCKKYPE IY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Replication factor-A from Saccharomyces cerevisiae is encoded by three essential genes coordinately expressed at S phase." Brill S.J., Stillman B. Genes Dev. 5:1589-1600(1991) [PubMed: 1885001] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 8-22; 29-46 AND 119-122. Strain: ATCC 208353 / W303-1A.
[2]	"Isolation of the TYE2 gene reveals its identity to SWI3 encoding a general transcription factor in Saccharomyces cerevisiae." Loehning C., Rosenbaum C., Ciriacy M. Curr. Genet. 24:193-199(1993) [PubMed: 8221926] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X." Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K. , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L. EMBO J. 15:2031-2049(1996) [PubMed: 8641269] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X59750 Genomic DNA. Translation: CAA42422.1. X56792 Genomic DNA. Translation: CAA40110.1. Z49448 Genomic DNA. Translation: CAA89468.1. AY558541 Genomic DNA. Translation: AAS56867.1. BK006943 Genomic DNA. Translation: DAA08631.1.
PIR	C37281.
RefSeq	NP_012362.1. NM_001181606.1.
3D structure databases
ProteinModelPortal	P26755.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-2109N.
IntAct	P26755. 20 interactions.
MINT	MINT-476189.
STRING	P26755.
Proteomic databases
PeptideAtlas	P26755.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YJL173C; YJL173C; YJL173C.
GeneID	853266.
KEGG	sce:YJL173C.
NMPDR	fig|4932.3.peg.3326.
Organism-specific databases
CYGD	YJL173c.
SGD	S000003709. RFA3.
Phylogenomic databases
eggNOG	fuNOG11746.
HOGENOM	HBG512047.
OMA	WYEFVCR.
OrthoDB	EOG4K0TZ3.
Gene expression databases
ArrayExpress	P26755.
Genevestigator	P26755.
GermOnline	YJL173C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR013970. Rep_factor-A_3. IPR016588. Rep_factor-A_su3. [Graphical view]
Pfam	PF08661. Rep_fac-A_3. 1 hit. [Graphical view]
PIRSF	PIRSF011773. Rep_factor-A_4. 1 hit.
ProtoNet	Search...
Other
NextBio	973532.

Entry information

Entry name

RFA3_YEAST

Accession

Primary (citable) accession number: P26755
Secondary accession number(s): D6VW15

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1992
Last sequence update:	August 1, 1992
Last modified:	December 14, 2011
This is version 94 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program