Replication factor A protein 3 - Saccharomyces cerevisiae (Baker's yeast)

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Reviewed, UniProtKB/Swiss-Prot P26755 (RFA3_YEAST)

Last modified November 24, 2009. Version 78. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
    Replication factor A protein 3
      Short name=RF-A
Alternative name(s):
    Replication protein A 13 kDa subunit

Gene names

Name:	RFA3
Ordered Locus Names:	YJL173C
ORF Names:	J0506

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

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Protein attributes

Sequence length	122 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Function	Binds single-stranded DNA. Stimulates the activity of a cognate strand exchange protein (SEP1). It cooperates with T-AG and DNA topoisomerase I to unwind template DNA containing the simian virus 40 origin of DNA replication.
Subunit structure	Heterotrimer of 69, 36, and 13 kDa chains. The DNA-binding activity may reside exclusively on the 69 kDa subunit.
Subcellular location	Nucleus.
Post-translational modification	The N-terminus is blocked.
Miscellaneous	Present with 4280 molecules/cell in log phase SD medium. Ref.5

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Ontologies

Keywords
Biological process	DNA replication
Cellular component	Nucleus
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	DNA unwinding during replication Inferred from direct assay. Source: SGD double-strand break repair via homologous recombination Inferred from genetic interaction. Source: SGD establishment of protein localization Inferred from physical interaction. Source: SGD heteroduplex formation Inferred from direct assay. Source: SGD nucleotide-excision repair Inferred from direct assay. Source: SGD protein ubiquitination Inferred from physical interaction. Source: SGD reciprocal meiotic recombination Inferred from physical interaction. Source: SGD telomere maintenance via recombination Inferred from physical interaction. Source: SGD telomere maintenance via telomerase Inferred from physical interaction. Source: SGD
Cellular component	DNA replication factor A complex Inferred from direct assay. Source: SGD chromosome, telomeric region Inferred from mutant phenotype. Source: SGD condensed nuclear chromosome Inferred from direct assay. Source: SGD
Molecular function	double-stranded DNA binding Inferred from direct assay. Source: SGD sequence-specific DNA binding Inferred from direct assay. Source: SGD
Complete GO annotation...

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 122

122

Replication factor A protein 3

PRO_0000097279

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Sequences

Sequence

Length

Mass (Da)

Tools

P26755-1 [UniParc].

Last modified August 1, 1992. Version 1.
Checksum: 5EC5D6FB56BAC008
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FASTA

122

13,816

        10         20         30         40         50         60 
MASETPRVDP TEISNVNAPV FRIIAQIKSQ PTESQLILQS PTISSKNGSE VEMITLNNIR 

        70         80         90        100        110        120 
VSMNKTFEID SWYEFVCRNN DDGELGFLIL DAVLCKFKEN EDLSLNGVVA LQRLCKKYPE 


IY

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Replication factor-A from Saccharomyces cerevisiae is encoded by three essential genes coordinately expressed at S phase." Brill S.J., Stillman B. Genes Dev. 5:1589-1600(1991) [PubMed: 1885001] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 8-22; 29-46 AND 119-122. Strain: ATCC 208353 / W303-1A.
[2]	"Isolation of the TYE2 gene reveals its identity to SWI3 encoding a general transcription factor in Saccharomyces cerevisiae." Loehning C., Rosenbaum C., Ciriacy M. Curr. Genet. 24:193-199(1993) [PubMed: 8221926] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X." Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K. Karpfinger-Hartl L. EMBO J. 15:2031-2049(1996) [PubMed: 8641269] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[4]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

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Cross-references

Sequence databases
	X59750 Genomic DNA. Translation: CAA42422.1. X56792 Genomic DNA. Translation: CAA40110.1. Z49448 Genomic DNA. Translation: CAA89468.1. AY558541 Genomic DNA. Translation: AAS56867.1.
PIR	C37281.
RefSeq	NP_012362.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:2109N.
IntAct	P26755. 25 interactions.
STRING	P26755.
Proteomic databases
PeptideAtlas	P26755.
PRIDE	P26755.
Genome annotation databases
Ensembl	YJL173C; YJL173C; YJL173C; Saccharomyces cerevisiae. [Genome view]
GeneID	853266.
KEGG	sce:YJL173C.
NMPDR	fig\|4932.3.peg.3326.
Organism-specific databases
CYGD	YJL173c.
SGD	S000003709. RFA3.
Phylogenomic databases
HOGENOM	P26755.
OMA	WYEFVCR
OrthoDB	EOG9M937S
Gene expression databases
ArrayExpress	P26755.
Genevestigator	P26755.
GermOnline	YJL173C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR016588. Rep_fac-A_su-3. IPR013970. Rep_factor-A_3. [Graphical view]
Pfam	PF08661. Rep_fac-A_3. 1 hit. [Graphical view]
PIRSF	PIRSF011773. Rep_factor-A_4. 1 hit.
ProtoNet	Search...
Other Resources
NextBio	973532.

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Entry information

Entry name

RFA3_YEAST

Accession

Primary (citable) accession number: P26755

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1992
Last sequence update:	August 1, 1992
Last modified:	November 24, 2009
This is version 78 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)

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Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome X

Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other Resources

Entry information

Relevant documents