P26725 (YUR1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable mannosyltransferase YUR1
EC=2.4.1.-

Gene names

Name:	YUR1
Ordered Locus Names:	YJL139C
ORF Names:	J0657

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	428 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Possible glycosyltransferase involved in N-linked glycosylation.
Catalytic activity	Transfers an alpha-D-mannosyl residue from GDP-mannose into lipid-linked oligosaccharide, forming an alpha-(1->2)-D-mannosyl-D-mannose linkage.
Pathway	Protein modification; protein glycosylation.
Subcellular location	Golgi apparatus membrane; Single-pass type II membrane protein.
Miscellaneous	Present with 6400 molecules/cell in log phase SD medium. Ref.5
Sequence similarities	Belongs to the glycosyltransferase 15 family.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Domain	Signal-anchor Transmembrane Transmembrane helix
Molecular function	Glycosyltransferase Transferase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cell wall mannoprotein biosynthetic process Inferred from mutant phenotype. Source: SGD protein N-linked glycosylation Inferred from mutant phenotype. Source: SGD
Cellular component	Golgi membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	mannosyltransferase activity Inferred from direct assay. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 428	428	Probable mannosyltransferase YUR1		PRO_0000208250
Regions
	Topological domain	1 – 3	3	Cytoplasmic Potential
	Transmembrane	4 – 24	21	Helical; Signal-anchor for type II membrane protein
	Topological domain	25 – 428	404	Lumenal Potential
	Region	25 – 88	64	Stem region By similarity
	Region	89 – 428	340	Catalytic By similarity
Sites
	Active site	313	1	Nucleophile Potential
Amino acid modifications
	Glycosylation	77	1	N-linked (GlcNAc...) Potential
	Glycosylation	82	1	N-linked (GlcNAc...) Potential
	Glycosylation	92	1	N-linked (GlcNAc...) Potential
	Glycosylation	167	1	N-linked (GlcNAc...) Potential
	Glycosylation	414	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	P26725 [UniParc]. Last modified August 1, 1992. Version 1. Checksum: E4F1CF97480DB3CA	FASTA	428	50,836
	10 20 30 40 50 60 MAKGGSLYIV GIFLPIWTFM IYIFGKELFL IRKYQKIDST YTALSQRVKE QYDTSRRRNY 70 80 90 100 110 120 FPKVKLSRNS YDDYTLNYTR QNDSDSFHLR ENATILMLVR NSELEGALDS MRSLEDRFNN 130 140 150 160 170 180 KYHYDWTFLN DVPFDQDFIE ATTSMASGKT QYALIPPEDW NRPQWINDTL FEERLRVMED 190 200 210 220 230 240 EGVLYGGSKS YRNMCRFNSG FFFRQSILDN YDYYFRVEPN VKYYCDFPYD PFRVMRLKGK 250 260 270 280 290 300 KYGFVISLYE YEETIPTLWD AVEEYLVASE ETILRKEDSA YAFLTDSGLV GKHYPVVEAN 310 320 330 340 350 360 SDYNLCHFWS NFEIGDLNFF RSDEYKHFFE TLDAKGGFYY ERWGDAPVHS IGVSLLLRPD 370 380 390 400 410 420 EIIHFDELGY FHSPFGTCPA SYAVRLDQRC RCKSDDESVI DITPHSCLMR WWKNGSGKYF LKEEQDEI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The Saccharomyces cerevisiae RPB4 gene is tightly linked to the TIF2 gene." Foreman P.K., Davis R.W., Sachs A.B. Nucleic Acids Res. 19:2781-2781(1991) [PubMed: 2041753] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Sequence analysis of a 40.7 kb segment from the left arm of yeast chromosome X reveals 14 known genes and 13 new open reading frames including homologues of genes clustered on the right arm of chromosome XI." Katsoulou C., Tzermia M., Tavernarakis N., Alexandraki D. Yeast 12:787-797(1996) [PubMed: 8813765] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X." Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K. , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L. EMBO J. 15:2031-2049(1996) [PubMed: 8641269] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X58099 Genomic DNA. Translation: CAA41111.1. X87371 Genomic DNA. Translation: CAA60816.1. Z49414 Genomic DNA. Translation: CAA89434.1. BK006943 Genomic DNA. Translation: DAA08661.1.
PIR	S36856.
RefSeq	NP_012396.1. NM_001181572.1.
3D structure databases
ProteinModelPortal	P26725.
SMR	P26725. Positions 83-418.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-2605N.
IntAct	P26725. 1 interaction.
MINT	MINT-422838.
STRING	P26725.
Protein family/group databases
CAZy	GT15. Glycosyltransferase Family 15.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YJL139C; YJL139C; YJL139C.
GeneID	853302.
KEGG	sce:YJL139C.
NMPDR	fig|4932.3.peg.3361.
Organism-specific databases
CYGD	YJL139c.
SGD	S000003675. YUR1.
Phylogenomic databases
eggNOG	fuNOG05651.
GeneTree	EFGT00050000003570.
HOGENOM	HBG396033.
OMA	VINNEIN.
OrthoDB	EOG4W11NQ.
Gene expression databases
ArrayExpress	P26725.
Genevestigator	P26725.
GermOnline	YJL139C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR002685. Glyco_trans_15. [Graphical view]
KO	K03854.
Pfam	PF01793. Glyco_transf_15. 1 hit. [Graphical view]
PIRSF	PIRSF018153. Glyco_trans_15. 1 hit.
ProtoNet	Search...
Other
NextBio	973624.

Entry information

Entry name

YUR1_YEAST

Accession

Primary (citable) accession number: P26725
Secondary accession number(s): D6VW45

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1992
Last sequence update:	August 1, 1992
Last modified:	December 14, 2011
This is version 107 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program