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Protein

Glutathione S-transferase 3

Gene
N/A
Organism
Gallus gallus (Chicken)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the conjugation of GSH to a wide variety of electrophilic alkylating agents. Also involved in the metabolism of lipid hydroperoxides, prostaglandins and leukotriene A4 and in binding of non-substrate hydrophobic ligands such as bile acids, a number of drugs and thyroid hormones. This GST does not exhibit peroxidase activity.

Catalytic activityi

RX + glutathione = HX + R-S-glutathione.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei9GlutathioneCombined sources1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Enzyme and pathway databases

SABIO-RKP26697.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutathione S-transferase 3 (EC:2.5.1.18)
Alternative name(s):
GST class-alpha
GST-CL3
OrganismiGallus gallus (Chicken)
Taxonomic identifieri9031 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiArchelosauriaArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalloanseraeGalliformesPhasianidaePhasianinaeGallus
Proteomesi
  • UP000000539 Componenti: Unplaced

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved
ChainiPRO_00001858002 – 229Glutathione S-transferase 3Add BLAST228

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2Blocked amino end (Ala)1

Proteomic databases

PaxDbiP26697.

Interactioni

Subunit structurei

Homodimer or heterodimer (with a subunit from group CL-4).

Protein-protein interaction databases

STRINGi9031.ENSGALP00000036177.

Structurei

Secondary structure

1229
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi6 – 9Combined sources4
Turni14 – 16Combined sources3
Helixi17 – 25Combined sources9
Beta strandi31 – 34Combined sources4
Helixi38 – 47Combined sources10
Beta strandi57 – 60Combined sources4
Beta strandi63 – 67Combined sources5
Helixi68 – 78Combined sources11
Helixi86 – 104Combined sources19
Turni105 – 107Combined sources3
Helixi109 – 111Combined sources3
Helixi114 – 130Combined sources17
Helixi132 – 143Combined sources12
Beta strandi146 – 149Combined sources4
Helixi155 – 170Combined sources16
Turni172 – 177Combined sources6
Helixi179 – 190Combined sources12
Helixi192 – 198Combined sources7
Helixi210 – 220Combined sources11

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1VF1X-ray1.77A1-229[»]
1VF2X-ray2.15A/B1-229[»]
1VF3X-ray2.15A/B1-229[»]
1VF4X-ray2.45A1-229[»]
ProteinModelPortaliP26697.
SMRiP26697.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP26697.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini3 – 83GST N-terminalAdd BLAST81
Domaini85 – 207GST C-terminalAdd BLAST123

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni54 – 55Glutathione bindingCombined sources2
Regioni67 – 68Glutathione bindingCombined sources2

Sequence similaritiesi

Belongs to the GST superfamily. Alpha family.Curated
Contains 1 GST C-terminal domain.Curated
Contains 1 GST N-terminal domain.Curated

Phylogenomic databases

eggNOGiKOG1695. Eukaryota.
ENOG4111VAU. LUCA.
HOGENOMiHOG000115734.
HOVERGENiHBG053749.
KOiK00799.
PhylomeDBiP26697.

Family and domain databases

Gene3Di1.20.1050.10. 1 hit.
3.40.30.10. 1 hit.
InterProiIPR010987. Glutathione-S-Trfase_C-like.
IPR004045. Glutathione_S-Trfase_N.
IPR003080. GST_alpha.
IPR004046. GST_C.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PfamiPF14497. GST_C_3. 1 hit.
PF02798. GST_N. 1 hit.
[Graphical view]
PRINTSiPR01266. GSTRNSFRASEA.
SUPFAMiSSF47616. SSF47616. 1 hit.
SSF52833. SSF52833. 1 hit.
PROSITEiPS50405. GST_CTER. 1 hit.
PS50404. GST_NTER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P26697-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAKPVLYYF NGRGKMESIR WLLAAAGVEF EEVFLETREQ YEKLLQSGIL
60 70 80 90 100
MFQQVPMVEI DGMKLVQTRA ILNYIAGKYN LYGKDLKERA LIDMYVGGTD
110 120 130 140 150
DLMGFLLSFP FLSAEDKVKQ CAFVVEKATS RYFPAYEKVL KDHGQDFLVG
160 170 180 190 200
NRLSWADIHL LEAILMVEEK KSDALSGFPL LQAFKKRISS IPTIKKFLAP
210 220
GSKRKPISDD KYVETVRRVL RMYYDVKPH
Length:229
Mass (Da):26,326
Last modified:January 23, 2007 - v2
Checksum:iEA30D949034BD8DB
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti45L → V.1
Natural varianti47S → A.1
Natural varianti49I → F.1
Natural varianti49I → V.1
Natural varianti52F → R.1
Natural varianti129 – 130TS → AN.2
Natural varianti135 – 136AY → VF.2
Natural varianti155W → R.1
Natural varianti158 – 159IH → VV.2
Natural varianti163A → T.1
Natural varianti166M → A.1
Natural varianti168E → V.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M38219 mRNA. Translation: AAA62731.1.
PIRiS19734.
RefSeqiNP_990743.1. NM_205412.1.
UniGeneiGga.788.

Genome annotation databases

GeneIDi396380.
KEGGigga:396380.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M38219 mRNA. Translation: AAA62731.1.
PIRiS19734.
RefSeqiNP_990743.1. NM_205412.1.
UniGeneiGga.788.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1VF1X-ray1.77A1-229[»]
1VF2X-ray2.15A/B1-229[»]
1VF3X-ray2.15A/B1-229[»]
1VF4X-ray2.45A1-229[»]
ProteinModelPortaliP26697.
SMRiP26697.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9031.ENSGALP00000036177.

Proteomic databases

PaxDbiP26697.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi396380.
KEGGigga:396380.

Phylogenomic databases

eggNOGiKOG1695. Eukaryota.
ENOG4111VAU. LUCA.
HOGENOMiHOG000115734.
HOVERGENiHBG053749.
KOiK00799.
PhylomeDBiP26697.

Enzyme and pathway databases

SABIO-RKP26697.

Miscellaneous databases

EvolutionaryTraceiP26697.
PROiP26697.

Family and domain databases

Gene3Di1.20.1050.10. 1 hit.
3.40.30.10. 1 hit.
InterProiIPR010987. Glutathione-S-Trfase_C-like.
IPR004045. Glutathione_S-Trfase_N.
IPR003080. GST_alpha.
IPR004046. GST_C.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PfamiPF14497. GST_C_3. 1 hit.
PF02798. GST_N. 1 hit.
[Graphical view]
PRINTSiPR01266. GSTRNSFRASEA.
SUPFAMiSSF47616. SSF47616. 1 hit.
SSF52833. SSF52833. 1 hit.
PROSITEiPS50405. GST_CTER. 1 hit.
PS50404. GST_NTER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGSTA3_CHICK
AccessioniPrimary (citable) accession number: P26697
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: January 23, 2007
Last modified: November 2, 2016
This is version 113 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

The variations were found from AA sequencing and imply there are multiple forms of CL-3.

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.