P26697 (GSTA3_CHICK) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Glutathione S-transferase 3 EC=2.5.1.18 Alternative name(s): GST class-alpha GST-CL3
Organism	Gallus gallus (Chicken) [Reference proteome]
Taxonomic identifier	9031 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Sauropsida › Sauria › Archosauria › Dinosauria › Saurischia › Theropoda › Coelurosauria › Aves › Neognathae › Galliformes › Phasianidae › Phasianinae › Gallus

Protein attributes

Sequence length	229 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the conjugation of GSH to a wide variety of electrophilic alkylating agents. Also involved in the metabolism of lipid hydroperoxides, prostaglandins and leukotriene A4 and in binding of non-substrate hydrophobic ligands such as bile acids, a number of drugs and thyroid hormones. This GST does not exhibit peroxidase activity.
Catalytic activity	RX + glutathione = HX + R-S-glutathione.
Subunit structure	Homodimer or heterodimer (with a subunit from group CL-4).
Subcellular location	Cytoplasm.
Miscellaneous	The variations were found from AA sequencing and imply there are multiple forms of CL-3.
Sequence similarities	Belongs to the GST superfamily. Alpha family. Contains 1 GST C-terminal domain. Contains 1 GST N-terminal domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Coding sequence diversity	Polymorphism
Molecular function	Transferase
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	glutathione metabolic process Inferred from sequence or structural similarity. Source: UniProtKB
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	glutathione transferase activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

1

1

Removed

Chain

2 – 229

228

Glutathione S-transferase 3

PRO_0000185800

Regions

Domain

3 – 83

81

GST N-terminal

Domain

85 – 207

123

GST C-terminal

Region

54 – 55

2

Glutathione binding

Region

67 – 68

2

Glutathione binding

Sites

Binding site

9

1

Glutathione

Amino acid modifications

Modified residue

2

1

Blocked amino end (Ala)

Natural variations

Natural variant

45

1

L → V.

Natural variant

47

1

S → A.

Natural variant

49

1

I → F.

Natural variant

49

1

I → V.

Natural variant

52

1

F → R.

Natural variant

129 – 130

2

TS → AN.

Natural variant

135 – 136

2

AY → VF.

Natural variant

155

1

W → R.

Natural variant

158 – 159

2

IH → VV.

Natural variant

163

1

A → T.

Natural variant

166

1

M → A.

Natural variant

168

1

E → V.

Secondary structure

1

.

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229

Helix Strand Turn

Details...

Beta strand

6 – 9

4

Turn

14 – 16

3

Helix

17 – 25

9

Beta strand

31 – 34

4

Helix

38 – 47

10

Beta strand

57 – 60

4

Beta strand

63 – 67

5

Helix

68 – 78

11

Helix

86 – 104

19

Turn

105 – 107

3

Helix

109 – 111

3

Helix

114 – 130

17

Helix

132 – 143

12

Beta strand

146 – 149

4

Helix

155 – 170

16

Turn

172 – 177

6

Helix

179 – 190

12

Helix

192 – 198

7

Helix

210 – 220

11

Sequences

	Sequence		Length	Mass (Da)
	P26697 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: EA30D949034BD8DB	FASTA	229	26,326
	10 20 30 40 50 60 MAAKPVLYYF NGRGKMESIR WLLAAAGVEF EEVFLETREQ YEKLLQSGIL MFQQVPMVEI 70 80 90 100 110 120 DGMKLVQTRA ILNYIAGKYN LYGKDLKERA LIDMYVGGTD DLMGFLLSFP FLSAEDKVKQ 130 140 150 160 170 180 CAFVVEKATS RYFPAYEKVL KDHGQDFLVG NRLSWADIHL LEAILMVEEK KSDALSGFPL 190 200 210 220 LQAFKKRISS IPTIKKFLAP GSKRKPISDD KYVETVRRVL RMYYDVKPH

References

[1]

"Cloning and expression of a chick liver glutathione S-transferase CL 3 subunit with the use of a baculovirus expression system." Chang L.-H., Fan J.-Y., Liu L.-F., Tsai S.-P., Tam M.F. Biochem. J. 281:545-551(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE. Strain: White leghorn. Tissue: Liver.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M38219 mRNA. Translation: AAA62731.1.

IPI

IPI00588923.

PIR

S19734.

RefSeq

NP_990743.1. NM_205412.1.

UniGene

Gga.788.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1VF1	X-ray	1.77	A	1-229	[»]
1VF2	X-ray	2.15	A/B	1-229	[»]
1VF3	X-ray	2.15	A/B	1-229	[»]
1VF4	X-ray	2.45	A	1-229	[»]

ProteinModelPortal

P26697.

SMR

P26697. Positions 2-228.

ModBase

Search...

Protein-protein interaction databases

STRING

9031.ENSGALP00000036177.

Proteomic databases

PaxDb

P26697.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

GeneID

396380.

KEGG

gga:396380.

Phylogenomic databases

eggNOG

NOG266414.

HOGENOM

HOG000115734.

HOVERGEN

HBG053749.

InParanoid

P26697.

KO

K00799.

OrthoDB

EOG4C2HBH.

Enzyme and pathway databases

SABIO-RK

P26697.

Family and domain databases

Gene3D

1.20.1050.10. 1 hit.
3.40.30.10. 1 hit.

InterPro

IPR010987. Glutathione-S-Trfase_C-like.
IPR004045. Glutathione_S-Trfase_N.
IPR017933. Glutathione_S_Trfase/Cl_chnl_C.
IPR003080. GST_alpha.
IPR004046. GST_C.
IPR012336. Thioredoxin-like_fold.
[Graphical view]

PANTHER

PTHR11571:SF4. PTHR11571:SF4. 1 hit.

Pfam

PF00043. GST_C. 1 hit.
PF02798. GST_N. 1 hit.
[Graphical view]

PRINTS

PR01266. GSTRNSFRASEA.

SUPFAM

SSF47616. GST_C_like. 1 hit.
SSF52833. Thiordxn-like_fd. 1 hit.

PROSITE

PS50405. GST_CTER. 1 hit.
PS50404. GST_NTER. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P26697.

NextBio

20816422.

Entry information

Entry name

GSTA3_CHICK

Accession

Primary (citable) accession number: P26697

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1992
Last sequence update:	January 23, 2007
Last modified:	April 3, 2013
This is version 92 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program