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P26696

- MK01_XENLA

UniProt

P26696 - MK01_XENLA

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Protein

Mitogen-activated protein kinase 1

Gene
mapk1, mpk1
Organism
Xenopus laevis (African clawed frog)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. Plays an important role in the MAPK/ERK cascade. Depending on the cellular context, this cascade mediates diverse biological functions such as cell growth, adhesion, survival and differentiation through the regulation of transcription, translation, cytoskeletal rearrangements. The MAPK/ERK cascade plays also a role in initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors. Many of the substrates are localized in the nucleus, and seem to participate in the regulation of transcription upon stimulation. However, other substrates are found in the cytosol as well as in other cellular organelles, and those are responsible for processes such as translation, mitosis and apoptosis. Moreover, the MAPK/ERK cascade is also involved in the regulation of the endosomal dynamics, including lysosome processing and endosome cycling through the perinuclear recycling compartment (PNRC); as well as in the fragmentation of the Golgi apparatus during mitosis. Phosphorylates microtubule-associated protein 2 (MAP2), myelin basic protein (MBP) and Elk-1. Phosphorylates dual specificity protein phosphatase 1 (DUSP1) during meiosis, increasing its stability. Activated by M phase promoting factor (MPF). Plays a role in the spindle assembly checkpoint.3 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Cofactori

Magnesium By similarity.

Enzyme regulationi

Activated by tyrosine phosphorylation during the M phase of the meiotic cell cycle. Dephosphorylated and inactivated by DUSP1.4 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei57 – 571ATP Inferred
Active sitei152 – 1521Proton acceptor By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi34 – 429ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. MAP kinase activity Source: UniProtKB-EC
  3. protein serine/threonine kinase activity Source: UniProtKB

GO - Biological processi

  1. apoptotic process Source: UniProtKB-KW
  2. mitotic spindle assembly checkpoint Source: UniProtKB
  3. peptidyl-serine phosphorylation Source: UniProtKB
  4. peptidyl-threonine phosphorylation Source: UniProtKB
  5. regulation of protein stability Source: UniProtKB
  6. response to epidermal growth factor Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Apoptosis, Cell cycle

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.11.24. 6726.

Names & Taxonomyi

Protein namesi
Recommended name:
Mitogen-activated protein kinase 1 (EC:2.7.11.24)
Short name:
MAP kinase 1
Short name:
MAPK 1
Alternative name(s):
M phase MAP kinase
Myelin basic protein kinase
Short name:
MBP kinase
Myelin xP42 protein kinase
Gene namesi
Name:mapk1
Synonyms:mpk1
OrganismiXenopus laevis (African clawed frog)
Taxonomic identifieri8355 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

Organism-specific databases

XenbaseiXB-GENE-865273. mapk1.

Subcellular locationi

Cytoplasmcytoskeletonmicrotubule organizing centercentrosome By similarity. Cytoplasm. Cytoplasmcytoskeletonspindle
Note: Associated with the spindle during prometaphase and metaphase in cultured XTC cells.1 Publication

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB
  2. microtubule organizing center Source: UniProtKB-SubCell
  3. mitotic spindle Source: UniProtKB
  4. nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi57 – 571K → R: Inactivation. 1 Publication
Mutagenesisi86 – 861I → Y: Inactivation. 1 Publication
Mutagenesisi188 – 1881T → V or D: No effect on Tyr phosphorylation. 1 Publication
Mutagenesisi190 – 1901Y → F: Affects Thr phosphorylation. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 361361Mitogen-activated protein kinase 1PRO_0000186250Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei188 – 1881Phosphothreonine1 Publication
Modified residuei190 – 1901Phosphotyrosine1 Publication

Post-translational modificationi

Dually phosphorylated on Thr-188 and Tyr-190, which activates the enzyme.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiP26696.

Expressioni

Tissue specificityi

Expressed in the central nervous system, kidney, liver, intestine and the hematopoietic system. Also found in heart, muscle, pancreas and lung.1 Publication

Developmental stagei

Is expressed in the early oocyte and is maintained at a constant level during embryogenesis. Its level declines at the mid-blastula transition.1 Publication

Interactioni

Protein-protein interaction databases

BioGridi100311. 3 interactions.
IntActiP26696. 1 interaction.
MINTiMINT-86973.

Structurei

3D structure databases

ProteinModelPortaliP26696.
SMRiP26696. Positions 18-361.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini28 – 316289Protein kinaseAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi188 – 1903TXY

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi2 – 65Poly-Ala

Domaini

The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

Sequence similaritiesi

Phylogenomic databases

HOVERGENiHBG014652.
KOiK04371.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR003527. MAP_kinase_CS.
IPR008349. MAPK_ERK1/2.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
PRINTSiPR01770. ERK1ERK2MAPK.
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS01351. MAPK. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P26696-1 [UniParc]FASTAAdd to Basket

« Hide

MAAAGAASNP GGGPEMVRGQ AFDVGPRYIN LAYIGEGAYG MVCSAHDNVN    50
KVRVAIKKIS PFEHQTYCQR TLREIKILLR FKHENIIGIN DIIRAPTIEQ 100
MKDVYIVQDL METDLYKLLK TQHLSNDHIC YFLYQILRGL KYIHSANVLH 150
RDLKPSNLLL NTTCDLKICD FGLARVADPD HDHTGFLTEY VATRWYRAPE 200
IMLNSKGYTK SIDIWSVGCI LAEMLSNRPI FPGKHYLDQL NHILGILGSP 250
SQEDLNCIIN LKARNYLLSL PHKNKVPWNR LFPNADPKAL DLLDKMLTFN 300
PHKRIEVEAA LAHPYLEQYY DPSDEPVAEA PFKFEMELDD LPKETLKELI 350
FEETARFQPG Y 361
Length:361
Mass (Da):41,257
Last modified:November 14, 2006 - v3
Checksum:iD14EFF145A183EC6
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti5 – 73GAA → AAS in AAA50002. 1 Publication
Sequence conflicti29 – 291I → T in AAA50002. 1 Publication
Sequence conflicti32 – 321A → S in AAA50002. 1 Publication
Sequence conflicti47 – 493DNV → CNI in AAA50002. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M60977 mRNA. Translation: AAA50002.1.
X59813 mRNA. Translation: CAA42482.1.
BC060748 mRNA. Translation: AAH60748.1.
PIRiA39754.
RefSeqiNP_001083548.1. NM_001090079.1.
UniGeneiXl.1680.
Xl.874.

Genome annotation databases

GeneIDi398985.
KEGGixla:398985.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M60977 mRNA. Translation: AAA50002.1 .
X59813 mRNA. Translation: CAA42482.1 .
BC060748 mRNA. Translation: AAH60748.1 .
PIRi A39754.
RefSeqi NP_001083548.1. NM_001090079.1.
UniGenei Xl.1680.
Xl.874.

3D structure databases

ProteinModelPortali P26696.
SMRi P26696. Positions 18-361.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 100311. 3 interactions.
IntActi P26696. 1 interaction.
MINTi MINT-86973.

Proteomic databases

PRIDEi P26696.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 398985.
KEGGi xla:398985.

Organism-specific databases

CTDi 398985.
Xenbasei XB-GENE-865273. mapk1.

Phylogenomic databases

HOVERGENi HBG014652.
KOi K04371.

Enzyme and pathway databases

BRENDAi 2.7.11.24. 6726.

Family and domain databases

InterProi IPR011009. Kinase-like_dom.
IPR003527. MAP_kinase_CS.
IPR008349. MAPK_ERK1/2.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view ]
Pfami PF00069. Pkinase. 1 hit.
[Graphical view ]
PRINTSi PR01770. ERK1ERK2MAPK.
SMARTi SM00220. S_TKc. 1 hit.
[Graphical view ]
SUPFAMi SSF56112. SSF56112. 1 hit.
PROSITEi PS01351. MAPK. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Tyrosine phosphorylation and activation of homologous protein kinases during oocyte maturation and mitogenic activation of fibroblasts."
    Posada J., Sanghera J., Pelech S., Aebersold R., Cooper J.A.
    Mol. Cell. Biol. 11:2517-2528(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Ovary.
  2. "Xenopus M phase MAP kinase: isolation of its cDNA and activation by MPF."
    Gotoh Y., Moriyama K., Matsuda S., Okumura E., Kishimoto T., Kawasaki H., Suzuki K., Yahara I., Sakai H., Nishida E.
    EMBO J. 10:2661-2668(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE, FUNCTION, ENZYME REGULATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
    Tissue: Oocyte.
  3. NIH - Xenopus Gene Collection (XGC) project
    Submitted (NOV-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Tail bud.
  4. "Requirements for phosphorylation of MAP kinase during meiosis in Xenopus oocytes."
    Posada J., Cooper J.A.
    Science 255:212-215(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION AT THR-188 AND TYR-190, MUTAGENESIS OF LYS-57; ILE-86; THR-188 AND TYR-190, ENZYME REGULATION.
  5. "A role for mitogen-activated protein kinase in the spindle assembly checkpoint in XTC cells."
    Wang X.M., Zhai Y., Ferrell J.E. Jr.
    J. Cell Biol. 137:433-443(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, ENZYME REGULATION.
  6. "Activation of p42 mitogen-activated protein kinase (MAPK), but not c-Jun NH(2)-terminal kinase, induces phosphorylation and stabilization of MAPK phosphatase XCL100 in Xenopus oocytes."
    Sohaskey M.L., Ferrell J.E. Jr.
    Mol. Biol. Cell 13:454-468(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, ENZYME REGULATION.

Entry informationi

Entry nameiMK01_XENLA
AccessioniPrimary (citable) accession number: P26696
Secondary accession number(s): Q5D061
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: November 14, 2006
Last modified: June 11, 2014
This is version 105 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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