P26696 (MK01_XENLA) Reviewed, UniProtKB/Swiss-Prot
Last modified June 11, 2014. Version 105. History...
Names and origin
|Protein names||Recommended name:|
Mitogen-activated protein kinase 1
Short name=MAP kinase 1
Short name=MAPK 1
M phase MAP kinase
Myelin basic protein kinase
Short name=MBP kinase
Myelin xP42 protein kinase
|Organism||Xenopus laevis (African clawed frog)|
|Taxonomic identifier||8355 [NCBI]|
|Taxonomic lineage||Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Amphibia › Batrachia › Anura › Pipoidea › Pipidae › Xenopodinae › Xenopus › Xenopus|
|Sequence length||361 AA.|
|Protein existence||Evidence at protein level|
General annotation (Comments)
Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. Plays an important role in the MAPK/ERK cascade. Depending on the cellular context, this cascade mediates diverse biological functions such as cell growth, adhesion, survival and differentiation through the regulation of transcription, translation, cytoskeletal rearrangements. The MAPK/ERK cascade plays also a role in initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors. Many of the substrates are localized in the nucleus, and seem to participate in the regulation of transcription upon stimulation. However, other substrates are found in the cytosol as well as in other cellular organelles, and those are responsible for processes such as translation, mitosis and apoptosis. Moreover, the MAPK/ERK cascade is also involved in the regulation of the endosomal dynamics, including lysosome processing and endosome cycling through the perinuclear recycling compartment (PNRC); as well as in the fragmentation of the Golgi apparatus during mitosis. Phosphorylates microtubule-associated protein 2 (MAP2), myelin basic protein (MBP) and Elk-1. Phosphorylates dual specificity protein phosphatase 1 (DUSP1) during meiosis, increasing its stability. Activated by M phase promoting factor (MPF). Plays a role in the spindle assembly checkpoint. Ref.2 Ref.5 Ref.6
ATP + a protein = ADP + a phosphoprotein.
Magnesium By similarity.
Cytoplasm › cytoskeleton › microtubule organizing center › centrosome By similarity. Cytoplasm. Cytoplasm › cytoskeleton › spindle. Note: Associated with the spindle during prometaphase and metaphase in cultured XTC cells. Ref.5
Expressed in the central nervous system, kidney, liver, intestine and the hematopoietic system. Also found in heart, muscle, pancreas and lung. Ref.2
Is expressed in the early oocyte and is maintained at a constant level during embryogenesis. Its level declines at the mid-blastula transition. Ref.2
The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
Dually phosphorylated on Thr-188 and Tyr-190, which activates the enzyme. Ref.4
Contains 1 protein kinase domain.
Sequence annotation (Features)
|Feature key||Position(s)||Length||Description||Graphical view||Feature identifier|
|Chain||1 – 361||361||Mitogen-activated protein kinase 1||PRO_0000186250|
|Domain||28 – 316||289||Protein kinase|
|Nucleotide binding||34 – 42||9||ATP By similarity|
|Motif||188 – 190||3||TXY|
|Compositional bias||2 – 6||5||Poly-Ala|
|Active site||152||1||Proton acceptor By similarity|
|Binding site||57||1||ATP Probable|
Amino acid modifications
|Modified residue||188||1||Phosphothreonine Ref.4|
|Modified residue||190||1||Phosphotyrosine Ref.4|
|Mutagenesis||57||1||K → R: Inactivation. Ref.4|
|Mutagenesis||86||1||I → Y: Inactivation. Ref.4|
|Mutagenesis||188||1||T → V or D: No effect on Tyr phosphorylation. Ref.4|
|Mutagenesis||190||1||Y → F: Affects Thr phosphorylation. Ref.4|
|Sequence conflict||5 – 7||3||GAA → AAS in AAA50002. Ref.1|
|Sequence conflict||29||1||I → T in AAA50002. Ref.1|
|Sequence conflict||32||1||A → S in AAA50002. Ref.1|
|Sequence conflict||47 – 49||3||DNV → CNI in AAA50002. Ref.1|
|||"Tyrosine phosphorylation and activation of homologous protein kinases during oocyte maturation and mitogenic activation of fibroblasts."|
Posada J., Sanghera J., Pelech S., Aebersold R., Cooper J.A.
Mol. Cell. Biol. 11:2517-2528(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
|||"Xenopus M phase MAP kinase: isolation of its cDNA and activation by MPF."|
Gotoh Y., Moriyama K., Matsuda S., Okumura E., Kishimoto T., Kawasaki H., Suzuki K., Yahara I., Sakai H., Nishida E.
EMBO J. 10:2661-2668(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE, FUNCTION, ENZYME REGULATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
|||NIH - Xenopus Gene Collection (XGC) project|
Submitted (NOV-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Tail bud.
|||"Requirements for phosphorylation of MAP kinase during meiosis in Xenopus oocytes."|
Posada J., Cooper J.A.
Science 255:212-215(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION AT THR-188 AND TYR-190, MUTAGENESIS OF LYS-57; ILE-86; THR-188 AND TYR-190, ENZYME REGULATION.
|||"A role for mitogen-activated protein kinase in the spindle assembly checkpoint in XTC cells."|
Wang X.M., Zhai Y., Ferrell J.E. Jr.
J. Cell Biol. 137:433-443(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, ENZYME REGULATION.
|||"Activation of p42 mitogen-activated protein kinase (MAPK), but not c-Jun NH(2)-terminal kinase, induces phosphorylation and stabilization of MAPK phosphatase XCL100 in Xenopus oocytes."|
Sohaskey M.L., Ferrell J.E. Jr.
Mol. Biol. Cell 13:454-468(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, ENZYME REGULATION.
|+||Additional computationally mapped references.|
|M60977 mRNA. Translation: AAA50002.1.|
X59813 mRNA. Translation: CAA42482.1.
BC060748 mRNA. Translation: AAH60748.1.
|RefSeq||NP_001083548.1. NM_001090079.1. |
3D structure databases
|SMR||P26696. Positions 18-361. |
Protein-protein interaction databases
|BioGrid||100311. 3 interactions.|
|IntAct||P26696. 1 interaction.|
Protocols and materials databases
Genome annotation databases
|Xenbase||XB-GENE-865273. mapk1. |
Enzyme and pathway databases
|BRENDA||18.104.22.168. 6726. |
Family and domain databases
|InterPro||IPR011009. Kinase-like_dom. |
|Pfam||PF00069. Pkinase. 1 hit. |
|PRINTS||PR01770. ERK1ERK2MAPK. |
|SMART||SM00220. S_TKc. 1 hit. |
|SUPFAM||SSF56112. SSF56112. 1 hit. |
|PROSITE||PS01351. MAPK. 1 hit. |
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
|Accession||Primary (citable) accession number: P26696|
Secondary accession number(s): Q5D061
|Entry status||Reviewed (UniProtKB/Swiss-Prot)|
|Annotation program||Chordata Protein Annotation Program|
Index of protein domains and families