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Protein

Mitogen-activated protein kinase 1

Gene

mapk1

Organism
Xenopus laevis (African clawed frog)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. Plays an important role in the MAPK/ERK cascade. Depending on the cellular context, this cascade mediates diverse biological functions such as cell growth, adhesion, survival and differentiation through the regulation of transcription, translation, cytoskeletal rearrangements. The MAPK/ERK cascade plays also a role in initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors. Many of the substrates are localized in the nucleus, and seem to participate in the regulation of transcription upon stimulation. However, other substrates are found in the cytosol as well as in other cellular organelles, and those are responsible for processes such as translation, mitosis and apoptosis. Moreover, the MAPK/ERK cascade is also involved in the regulation of the endosomal dynamics, including lysosome processing and endosome cycling through the perinuclear recycling compartment (PNRC); as well as in the fragmentation of the Golgi apparatus during mitosis. Phosphorylates microtubule-associated protein 2 (MAP2), myelin basic protein (MBP) and Elk-1. Phosphorylates dual specificity protein phosphatase 1 (DUSP1) during meiosis, increasing its stability. Activated by M phase promoting factor (MPF). Plays a role in the spindle assembly checkpoint.3 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Cofactori

Mg2+By similarity

Enzyme regulationi

Activated by tyrosine phosphorylation during the M phase of the meiotic cell cycle. Dephosphorylated and inactivated by DUSP1.4 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei57ATPCurated1
Active sitei152Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi34 – 42ATPPROSITE-ProRule annotation9

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase
Biological processApoptosis, Cell cycle
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.11.24. 6725.

Names & Taxonomyi

Protein namesi
Recommended name:
Mitogen-activated protein kinase 1 (EC:2.7.11.24)
Short name:
MAP kinase 1
Short name:
MAPK 1
Alternative name(s):
M phase MAP kinase
Myelin basic protein kinase
Short name:
MBP kinase
Myelin xP42 protein kinase
Gene namesi
Name:mapk1
Synonyms:mpk1
OrganismiXenopus laevis (African clawed frog)
Taxonomic identifieri8355 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

Organism-specific databases

XenbaseiXB-GENE-865273. mapk1.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi57K → R: Inactivation. 1 Publication1
Mutagenesisi86I → Y: Inactivation. 1 Publication1
Mutagenesisi188T → V or D: No effect on Tyr phosphorylation. 1 Publication1
Mutagenesisi190Y → F: Affects Thr phosphorylation. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001862501 – 361Mitogen-activated protein kinase 1Add BLAST361

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei188Phosphothreonine1 Publication1
Modified residuei190Phosphotyrosine1 Publication1

Post-translational modificationi

Dually phosphorylated on Thr-188 and Tyr-190, which activates the enzyme.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP26696.
PRIDEiP26696.

PTM databases

iPTMnetiP26696.

Expressioni

Tissue specificityi

Expressed in the central nervous system, kidney, liver, intestine and the hematopoietic system. Also found in heart, muscle, pancreas and lung.1 Publication

Developmental stagei

Is expressed in the early oocyte and is maintained at a constant level during embryogenesis. Its level declines at the mid-blastula transition.1 Publication

Interactioni

Subunit structurei

Interacts with CDK2AP2 (PubMed:12944431).1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
rps6kaP106652EBI-6957733,EBI-6957709

Protein-protein interaction databases

BioGridi100311. 3 interactors.
IntActiP26696. 2 interactors.
MINTiMINT-86973.

Structurei

3D structure databases

ProteinModelPortaliP26696.
SMRiP26696.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini28 – 316Protein kinasePROSITE-ProRule annotationAdd BLAST289

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi188 – 190TXY3

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi2 – 6Poly-Ala5

Domaini

The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

Sequence similaritiesi

Phylogenomic databases

HOVERGENiHBG014652.
KOiK04371.

Family and domain databases

InterProiView protein in InterPro
IPR011009. Kinase-like_dom.
IPR003527. MAP_kinase_CS.
IPR008349. MAPK_ERK1/2.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
PfamiView protein in Pfam
PF00069. Pkinase. 1 hit.
PRINTSiPR01770. ERK1ERK2MAPK.
SMARTiView protein in SMART
SM00220. S_TKc. 1 hit.
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiView protein in PROSITE
PS01351. MAPK. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.

Sequencei

Sequence statusi: Complete.

P26696-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAAGAASNP GGGPEMVRGQ AFDVGPRYIN LAYIGEGAYG MVCSAHDNVN
60 70 80 90 100
KVRVAIKKIS PFEHQTYCQR TLREIKILLR FKHENIIGIN DIIRAPTIEQ
110 120 130 140 150
MKDVYIVQDL METDLYKLLK TQHLSNDHIC YFLYQILRGL KYIHSANVLH
160 170 180 190 200
RDLKPSNLLL NTTCDLKICD FGLARVADPD HDHTGFLTEY VATRWYRAPE
210 220 230 240 250
IMLNSKGYTK SIDIWSVGCI LAEMLSNRPI FPGKHYLDQL NHILGILGSP
260 270 280 290 300
SQEDLNCIIN LKARNYLLSL PHKNKVPWNR LFPNADPKAL DLLDKMLTFN
310 320 330 340 350
PHKRIEVEAA LAHPYLEQYY DPSDEPVAEA PFKFEMELDD LPKETLKELI
360
FEETARFQPG Y
Length:361
Mass (Da):41,257
Last modified:November 14, 2006 - v3
Checksum:iD14EFF145A183EC6
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti5 – 7GAA → AAS in AAA50002 (PubMed:1708093).Curated3
Sequence conflicti29I → T in AAA50002 (PubMed:1708093).Curated1
Sequence conflicti32A → S in AAA50002 (PubMed:1708093).Curated1
Sequence conflicti47 – 49DNV → CNI in AAA50002 (PubMed:1708093).Curated3

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M60977 mRNA. Translation: AAA50002.1.
X59813 mRNA. Translation: CAA42482.1.
BC060748 mRNA. Translation: AAH60748.1.
PIRiA39754.
RefSeqiNP_001083548.1. NM_001090079.1.
UniGeneiXl.1680.
Xl.874.

Genome annotation databases

GeneIDi398985.
KEGGixla:398985.

Similar proteinsi

Entry informationi

Entry nameiMK01_XENLA
AccessioniPrimary (citable) accession number: P26696
Secondary accession number(s): Q5D061
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: November 14, 2006
Last modified: September 27, 2017
This is version 123 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. SIMILARITY comments
    Index of protein domains and families