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P26696

- MK01_XENLA

UniProt

P26696 - MK01_XENLA

Protein

Mitogen-activated protein kinase 1

Gene

mapk1

Organism
Xenopus laevis (African clawed frog)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 106 (01 Oct 2014)
      Sequence version 3 (14 Nov 2006)
      Previous versions | rss
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    Functioni

    Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. Plays an important role in the MAPK/ERK cascade. Depending on the cellular context, this cascade mediates diverse biological functions such as cell growth, adhesion, survival and differentiation through the regulation of transcription, translation, cytoskeletal rearrangements. The MAPK/ERK cascade plays also a role in initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors. Many of the substrates are localized in the nucleus, and seem to participate in the regulation of transcription upon stimulation. However, other substrates are found in the cytosol as well as in other cellular organelles, and those are responsible for processes such as translation, mitosis and apoptosis. Moreover, the MAPK/ERK cascade is also involved in the regulation of the endosomal dynamics, including lysosome processing and endosome cycling through the perinuclear recycling compartment (PNRC); as well as in the fragmentation of the Golgi apparatus during mitosis. Phosphorylates microtubule-associated protein 2 (MAP2), myelin basic protein (MBP) and Elk-1. Phosphorylates dual specificity protein phosphatase 1 (DUSP1) during meiosis, increasing its stability. Activated by M phase promoting factor (MPF). Plays a role in the spindle assembly checkpoint.3 Publications

    Catalytic activityi

    ATP + a protein = ADP + a phosphoprotein.

    Cofactori

    Magnesium.By similarity

    Enzyme regulationi

    Activated by tyrosine phosphorylation during the M phase of the meiotic cell cycle. Dephosphorylated and inactivated by DUSP1.4 Publications

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei57 – 571ATPCurated
    Active sitei152 – 1521Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi34 – 429ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. MAP kinase activity Source: UniProtKB-EC
    3. protein serine/threonine kinase activity Source: UniProtKB

    GO - Biological processi

    1. apoptotic process Source: UniProtKB-KW
    2. mitotic spindle assembly checkpoint Source: UniProtKB
    3. peptidyl-serine phosphorylation Source: UniProtKB
    4. peptidyl-threonine phosphorylation Source: UniProtKB
    5. regulation of protein stability Source: UniProtKB
    6. response to epidermal growth factor Source: UniProtKB

    Keywords - Molecular functioni

    Kinase, Serine/threonine-protein kinase, Transferase

    Keywords - Biological processi

    Apoptosis, Cell cycle

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BRENDAi2.7.11.24. 6726.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Mitogen-activated protein kinase 1 (EC:2.7.11.24)
    Short name:
    MAP kinase 1
    Short name:
    MAPK 1
    Alternative name(s):
    M phase MAP kinase
    Myelin basic protein kinase
    Short name:
    MBP kinase
    Myelin xP42 protein kinase
    Gene namesi
    Name:mapk1
    Synonyms:mpk1
    OrganismiXenopus laevis (African clawed frog)
    Taxonomic identifieri8355 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

    Organism-specific databases

    XenbaseiXB-GENE-865273. mapk1.

    Subcellular locationi

    Cytoplasmcytoskeletonmicrotubule organizing centercentrosome By similarity. Cytoplasm 1 Publication. Cytoplasmcytoskeletonspindle 1 Publication
    Note: Associated with the spindle during prometaphase and metaphase in cultured XTC cells.

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB
    2. microtubule organizing center Source: UniProtKB-SubCell
    3. mitotic spindle Source: UniProtKB
    4. nucleus Source: UniProtKB

    Keywords - Cellular componenti

    Cytoplasm, Cytoskeleton

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi57 – 571K → R: Inactivation. 1 Publication
    Mutagenesisi86 – 861I → Y: Inactivation. 1 Publication
    Mutagenesisi188 – 1881T → V or D: No effect on Tyr phosphorylation. 1 Publication
    Mutagenesisi190 – 1901Y → F: Affects Thr phosphorylation. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 361361Mitogen-activated protein kinase 1PRO_0000186250Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei188 – 1881Phosphothreonine1 Publication
    Modified residuei190 – 1901Phosphotyrosine1 Publication

    Post-translational modificationi

    Dually phosphorylated on Thr-188 and Tyr-190, which activates the enzyme.1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PRIDEiP26696.

    Expressioni

    Tissue specificityi

    Expressed in the central nervous system, kidney, liver, intestine and the hematopoietic system. Also found in heart, muscle, pancreas and lung.1 Publication

    Developmental stagei

    Is expressed in the early oocyte and is maintained at a constant level during embryogenesis. Its level declines at the mid-blastula transition.1 Publication

    Interactioni

    Protein-protein interaction databases

    BioGridi100311. 3 interactions.
    IntActiP26696. 1 interaction.
    MINTiMINT-86973.

    Structurei

    3D structure databases

    ProteinModelPortaliP26696.
    SMRiP26696. Positions 18-361.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini28 – 316289Protein kinasePROSITE-ProRule annotationAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi188 – 1903TXY

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi2 – 65Poly-Ala

    Domaini

    The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

    Sequence similaritiesi

    Contains 1 protein kinase domain.PROSITE-ProRule annotation

    Phylogenomic databases

    HOVERGENiHBG014652.
    KOiK04371.

    Family and domain databases

    InterProiIPR011009. Kinase-like_dom.
    IPR003527. MAP_kinase_CS.
    IPR008349. MAPK_ERK1/2.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view]
    PfamiPF00069. Pkinase. 1 hit.
    [Graphical view]
    PRINTSiPR01770. ERK1ERK2MAPK.
    SMARTiSM00220. S_TKc. 1 hit.
    [Graphical view]
    SUPFAMiSSF56112. SSF56112. 1 hit.
    PROSITEiPS01351. MAPK. 1 hit.
    PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P26696-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAAAGAASNP GGGPEMVRGQ AFDVGPRYIN LAYIGEGAYG MVCSAHDNVN    50
    KVRVAIKKIS PFEHQTYCQR TLREIKILLR FKHENIIGIN DIIRAPTIEQ 100
    MKDVYIVQDL METDLYKLLK TQHLSNDHIC YFLYQILRGL KYIHSANVLH 150
    RDLKPSNLLL NTTCDLKICD FGLARVADPD HDHTGFLTEY VATRWYRAPE 200
    IMLNSKGYTK SIDIWSVGCI LAEMLSNRPI FPGKHYLDQL NHILGILGSP 250
    SQEDLNCIIN LKARNYLLSL PHKNKVPWNR LFPNADPKAL DLLDKMLTFN 300
    PHKRIEVEAA LAHPYLEQYY DPSDEPVAEA PFKFEMELDD LPKETLKELI 350
    FEETARFQPG Y 361
    Length:361
    Mass (Da):41,257
    Last modified:November 14, 2006 - v3
    Checksum:iD14EFF145A183EC6
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti5 – 73GAA → AAS in AAA50002. (PubMed:1708093)Curated
    Sequence conflicti29 – 291I → T in AAA50002. (PubMed:1708093)Curated
    Sequence conflicti32 – 321A → S in AAA50002. (PubMed:1708093)Curated
    Sequence conflicti47 – 493DNV → CNI in AAA50002. (PubMed:1708093)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M60977 mRNA. Translation: AAA50002.1.
    X59813 mRNA. Translation: CAA42482.1.
    BC060748 mRNA. Translation: AAH60748.1.
    PIRiA39754.
    RefSeqiNP_001083548.1. NM_001090079.1.
    UniGeneiXl.1680.
    Xl.874.

    Genome annotation databases

    GeneIDi398985.
    KEGGixla:398985.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M60977 mRNA. Translation: AAA50002.1 .
    X59813 mRNA. Translation: CAA42482.1 .
    BC060748 mRNA. Translation: AAH60748.1 .
    PIRi A39754.
    RefSeqi NP_001083548.1. NM_001090079.1.
    UniGenei Xl.1680.
    Xl.874.

    3D structure databases

    ProteinModelPortali P26696.
    SMRi P26696. Positions 18-361.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 100311. 3 interactions.
    IntActi P26696. 1 interaction.
    MINTi MINT-86973.

    Proteomic databases

    PRIDEi P26696.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 398985.
    KEGGi xla:398985.

    Organism-specific databases

    CTDi 398985.
    Xenbasei XB-GENE-865273. mapk1.

    Phylogenomic databases

    HOVERGENi HBG014652.
    KOi K04371.

    Enzyme and pathway databases

    BRENDAi 2.7.11.24. 6726.

    Family and domain databases

    InterProi IPR011009. Kinase-like_dom.
    IPR003527. MAP_kinase_CS.
    IPR008349. MAPK_ERK1/2.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view ]
    Pfami PF00069. Pkinase. 1 hit.
    [Graphical view ]
    PRINTSi PR01770. ERK1ERK2MAPK.
    SMARTi SM00220. S_TKc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56112. SSF56112. 1 hit.
    PROSITEi PS01351. MAPK. 1 hit.
    PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Tyrosine phosphorylation and activation of homologous protein kinases during oocyte maturation and mitogenic activation of fibroblasts."
      Posada J., Sanghera J., Pelech S., Aebersold R., Cooper J.A.
      Mol. Cell. Biol. 11:2517-2528(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Ovary.
    2. "Xenopus M phase MAP kinase: isolation of its cDNA and activation by MPF."
      Gotoh Y., Moriyama K., Matsuda S., Okumura E., Kishimoto T., Kawasaki H., Suzuki K., Yahara I., Sakai H., Nishida E.
      EMBO J. 10:2661-2668(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE, FUNCTION, ENZYME REGULATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
      Tissue: Oocyte.
    3. NIH - Xenopus Gene Collection (XGC) project
      Submitted (NOV-2003) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Tail bud.
    4. "Requirements for phosphorylation of MAP kinase during meiosis in Xenopus oocytes."
      Posada J., Cooper J.A.
      Science 255:212-215(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION AT THR-188 AND TYR-190, MUTAGENESIS OF LYS-57; ILE-86; THR-188 AND TYR-190, ENZYME REGULATION.
    5. "A role for mitogen-activated protein kinase in the spindle assembly checkpoint in XTC cells."
      Wang X.M., Zhai Y., Ferrell J.E. Jr.
      J. Cell Biol. 137:433-443(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION, ENZYME REGULATION.
    6. "Activation of p42 mitogen-activated protein kinase (MAPK), but not c-Jun NH(2)-terminal kinase, induces phosphorylation and stabilization of MAPK phosphatase XCL100 in Xenopus oocytes."
      Sohaskey M.L., Ferrell J.E. Jr.
      Mol. Biol. Cell 13:454-468(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, ENZYME REGULATION.

    Entry informationi

    Entry nameiMK01_XENLA
    AccessioniPrimary (citable) accession number: P26696
    Secondary accession number(s): Q5D061
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1992
    Last sequence update: November 14, 2006
    Last modified: October 1, 2014
    This is version 106 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Direct protein sequencing

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3