Reviewed,
UniProtKB/Swiss-Prot P26692 (ACDA_METSO)
Last modified
June 16, 2009.
Version 61.
History...
Clusters with 100%,
90%,
50% identity |
 Documents (1) |
 Third-party data |
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Names and origin
| Protein names | Recommended name: Acetyl-CoA decarbonylase/synthase complex subunit alpha Short name=ACDS complex subunit alpha EC=1.2.99.2 Alternative name(s): ACDS complex carbon monoxide dehydrogenase Short name=ACDS CODH | ||
| Gene names |
| ||
| Organism | Methanothrix soehngenii | ||
| Taxonomic identifier | 2223 [NCBI] | ||
| Taxonomic lineage | Archaea › Euryarchaeota › Methanomicrobia › Methanosarcinales › Methanosaetaceae › Methanosaeta |
Protein attributes
| Sequence length | 793 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Part of a complex that catalyzes the reversible cleavage of acetyl-CoA, allowing autotrophic growth from CO2 By similarity. |
| Catalytic activity | CO + H2O + A = CO2 + AH2. HAMAP MF_01137 |
| Cofactor | Binds 7 4Fe-4S clusters per heterotetramer Potential. Binds 2 nickel-iron-sulfur clusters per heterotetramer Potential. |
| Subunit structure | Heterotetramer of two alpha and two epsilon chains. The ACDS complex is made up of alpha, epsilon, beta, gamma and delta chains with a probable stoichiometry of (alpha2epsilon2)(4)-beta(8)-(gamma1delta1)8 Potential. |
| Domain | Cluster B is an all-cysteinyl-liganded 4Fe4S cluster; cluster C is a mixed Ni-Fe-S cluster which appears to be the active site of CO oxidation. Cluster D is also an all-cysteinyl-liganded 4Fe4S cluster that bridges the two subunits of the CODH dimer. May contain two additional 4Fe-4S clusters, dubbed E and F, that might reroute electron transfer along different paths. HAMAP MF_01137 |
| Sequence similarities | Belongs to the Ni-containing carbon monoxide dehydrogenase family. Contains 2 4Fe-4S ferredoxin-type domains. |
| Caution | This protein lacks the conserved Cys in positions 52 and 56; they are replaced by an Asp and a Thr, respectively. It is therefore possible that the C- and D-clusters are either altered or missing in this protein, which may not form heterotetramers. |
Ontologies
| Keywords | |
|---|---|
| Domain | Repeat |
| Ligand | 4Fe-4S Iron Iron-sulfur Metal-binding Nickel |
| Molecular function | Oxidoreductase |
| Technical term | Direct protein sequencing |
| Gene Ontology (GO) | |
| Biological process | acetyl-CoA metabolic process Inferred from electronic annotation. Source: InterPro generation of precursor metabolites and energyInferred from electronic annotation. Source: InterPro oxidation reductionInferred from electronic annotation. Source: UniProtKB-KW |
| Cellular component | cytoplasm Inferred from electronic annotation. Source: InterPro |
| Molecular function | 4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW carbon-monoxide dehydrogenase (acceptor) activityInferred from electronic annotation. Source: HAMAP electron carrier activityInferred from electronic annotation. Source: InterPro iron ion bindingInferred from electronic annotation. Source: UniProtKB-KW nickel ion bindingInferred from electronic annotation. Source: UniProtKB-KW |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 793 | 793 | Acetyl-CoA decarbonylase/synthase complex subunit alpha HAMAP MF_01137 | PRO_0000155082 | |||||
Regions | |||||||||
| Domain | 393 – 422 | 30 | 4Fe-4S ferredoxin-type 1 | ||||||
| Domain | 432 – 461 | 30 | 4Fe-4S ferredoxin-type 2 | ||||||
Sites | |||||||||
| Metal binding | 55 | 1 | Iron-sulfur 1 (4Fe-4S); shared with dimeric partner By similarity | ||||||
| Metal binding | 58 | 1 | Iron-sulfur 2 (4Fe-4S) By similarity | ||||||
| Metal binding | 63 | 1 | Iron-sulfur 2 (4Fe-4S) By similarity | ||||||
| Metal binding | 73 | 1 | Iron-sulfur 2 (4Fe-4S) By similarity | ||||||
| Metal binding | 229 | 1 | Nickel-iron-sulfur By similarity | ||||||
| Metal binding | 257 | 1 | Nickel-iron-sulfur By similarity | ||||||
| Metal binding | 309 | 1 | Nickel-iron-sulfur By similarity | ||||||
| Metal binding | 403 | 1 | Iron-sulfur 3 (4Fe-4S) Potential | ||||||
| Metal binding | 406 | 1 | Iron-sulfur 3 (4Fe-4S) Potential | ||||||
| Metal binding | 409 | 1 | Iron-sulfur 3 (4Fe-4S) Potential | ||||||
| Metal binding | 413 | 1 | Iron-sulfur 3 (4Fe-4S) Potential | ||||||
| Metal binding | 441 | 1 | Iron-sulfur 4 (4Fe-4S) Potential | ||||||
| Metal binding | 444 | 1 | Iron-sulfur 4 (4Fe-4S) Potential | ||||||
| Metal binding | 447 | 1 | Iron-sulfur 4 (4Fe-4S) Potential | ||||||
| Metal binding | 451 | 1 | Iron-sulfur 4 (4Fe-4S) Potential | ||||||
| Metal binding | 509 | 1 | Nickel-iron-sulfur By similarity | ||||||
| Metal binding | 538 | 1 | Nickel-iron-sulfur By similarity | ||||||
| Metal binding | 573 | 1 | Nickel-iron-sulfur By similarity | ||||||
Sequences
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References
| [1] | "Cloning, expression, and sequence analysis of the genes for carbon monoxide dehydrogenase of Methanothrix soehngenii." Eggen R.I.L., Geerling A.C.M., Jetten M.S.M., de Vos W.M. J. Biol. Chem. 266:6883-6887(1991) [PubMed: 1901858] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE. Strain: Opfikon / DSM 2139. |
Cross-references
Sequence databases | |
|---|---|
| M55280 Genomic DNA. Translation: AAA72934.1. | |
| PIR | A39764. |
3D structure databases | |
| ModBase | Search... |
Enzyme and pathway databases | |
| BRENDA | 1.2.99.2. 267. |
Family and domain databases | |
| HAMAP | MF_01137. [Tree] |
| InterPro | IPR017896. 4Fe4S_Fe-S-bd. IPR001450. 4Fe4S_Fe_S_bd_subgr. IPR017900. 4Fe4S_Fe_S_CS. IPR004460. CO_DH/Ac-CoA_synth_asu. IPR016101. CO_DH_a-bundle. IPR004137. Prismane. IPR016099. Prismane-like_a/b-sand. [Graphical view] |
| Gene3D | G3DSA:1.20.1270.30. CO_DH_a-bundle. 1 hit. G3DSA:3.40.50.2030. Prismane-like_a/b-sand. 2 hits. |
| Pfam | PF00037. Fer4. 1 hit. PF03063. Prismane. 1 hit. [Graphical view] |
| TIGRFAMs | TIGR00314. cdhA. 1 hit. |
| PROSITE | PS00198. 4FE4S_FER_1. 2 hits. PS51379. 4FE4S_FER_2. 2 hits. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | ACDA_METSO | ||||||||
| Accession | Primary (citable) accession number: P26692 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes) | ||||||||
