Reviewed, UniProtKB/Swiss-Prot P26690 (6DCS_SOYBN)

Names and origin

Protein names	Recommended name:     NAD(P)H-dependent 6'-deoxychalcone synthase     EC=2.3.1.170
Organism	Glycine max (Soybean)
Taxonomic identifier	3847 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids I › Fabales › Fabaceae › Papilionoideae › Phaseoleae › Glycine

Protein attributes

Sequence length	315 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Co-acts with chalcone synthase in formation of 4,2',4'-trihydroxychalcone, involved in the biosynthesis of glyceollin type phytoalexins.
Catalytic activity	3 malonyl-CoA + 4-coumaroyl-CoA + NADPH = 4 CoA + isoliquiritigenin + 3 CO2 + NADP+ + H2O.
Pathway	Phytoalexin biosynthesis; glyceollin biosynthesis.
Subunit structure	Monomer.
Induction	By pathogen infection.
Sequence similarities	Belongs to the aldo/keto reductase family.

Ontologies

Keywords
Biological process	Flavonoid biosynthesis
Ligand	NADP
Molecular function	Transferase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	flavonoid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: InterPro
Molecular function	6'-deoxychalcone synthase activity Inferred from electronic annotation. Source: EC oxidoreductase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 315	315	NAD(P)H-dependent 6'-deoxychalcone synthase		PRO_0000124609
Regions
	Nucleotide binding	216 – 274	59	NADP By similarity
Sites
	Active site	59	1	Proton donor By similarity
	Binding site	121	1	Substrate By similarity
	Site	88	1	Lowers pKa of active site Tyr By similarity

Sequences

	Sequence		Length	Mass (Da)
	P26690-1 [UniParc]. Last modified August 1, 1992. Version 1. Checksum: C3A6BE07EF330F47	FASTA	315	35,490
	10 20 30 40 50 60 MAAAIEIPTI VFPNSSAQQR MPVVGMGSAP DFTCKKDTKE AIIEAVKQGY RHFDTAAAYG 70 80 90 100 110 120 SEQALGEALK EAIHLGLVSR QDLFVTSKLW VTENHPHLVL PALRKSLKTL QLEYLDLYLI 130 140 150 160 170 180 HWPLSSQPGK FSFPIEVEDL LPFDVKGVWE SMEECQKLGL TKAIGVSNFS VKKLQNLLSV 190 200 210 220 230 240 ATIRPVVDQV EMNLAWQQKK LREFCKENGI IVTAFSPLRK GASRGPNEVM ENDVLKEIAE 250 260 270 280 290 300 AHGKSIAQVS LRWLYEQGVT FVPKSYDKER MNQNLHIFDW ALTEQDHHKI SQISQSRLIS 310 GPTKPQLADL WDDQI

References

[1]

"Induced plant responses to pathogen attack. Analysis and heterologous expression of the key enzyme in the biosynthesis of phytoalexins in soybean (Glycine max L. Merr. cv. Harosoy 63)." Welle R., Schroeder G., Schiltz E., Grisebach H., Schroeder J. Eur. J. Biochem. 196:423-430(1991) [PubMed: 1840523] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE. Strain: cv. Harosoy 63.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X55730 mRNA. Translation: CAA39261.1.
PIR	S14222.
UniGene	Gma.30860
3D structure databases
HSSP	HSSP built from PDB template 1J96 based on UniProtKB P52895.
SMR	P26690. Positions 6-313.
ModBase	Search...
Enzyme and pathway databases
BRENDA	2.3.1.170. 299.
Family and domain databases
InterPro	IPR001395. Aldo/ket_red. IPR018170. Aldo/ket_reductase_CS. [Graphical view]
Gene3D	G3DSA:3.20.20.100. Aldo/ket_red. 1 hit.
PANTHER	PTHR11732. Aldo/ket_red. 1 hit.
Pfam	PF00248. Aldo_ket_red. 1 hit. [Graphical view]
ProDom	PD000288. Aldo/ket_red. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00798. ALDOKETO_REDUCTASE_1. 1 hit. PS00062. ALDOKETO_REDUCTASE_2. 1 hit. PS00063. ALDOKETO_REDUCTASE_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

6DCS_SOYBN

Accession

Primary (citable) accession number: P26690

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1992
Last sequence update:	August 1, 1992
Last modified:	March 24, 2009
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)