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P26685

- RIR1_ASFM2

UniProt

P26685 - RIR1_ASFM2

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Protein

Ribonucleoside-diphosphate reductase large subunit

Gene
Mal-053
Organism
African swine fever virus (isolate Tick/Malawi/Lil 20-1/1983) (ASFV)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Ribonucleoside-diphosphate reductase holoenzyme provides the precursors necessary for viral DNA synthesis. Allows virus growth in non-dividing cells. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides By similarity.

Catalytic activityi

2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.

Enzyme regulationi

Under complex allosteric control mediated by deoxynucleoside triphosphates and ATP binding. The type of nucleotide bound at the specificity site determines substrate preference. It seems probable that ATP makes the enzyme reduce CDP and UDP, dGTP favors ADP reduction and dTTP favors GDP reduction By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei178 – 1781Substrate By similarity
Sitei194 – 1941Important for hydrogen atom transfer By similarity
Sitei201 – 2011Allosteric effector binding By similarity
Binding sitei222 – 2221Substrate; via amide nitrogen By similarity
Sitei231 – 2311Allosteric effector binding By similarity
Active sitei420 – 4201Proton acceptor By similarity
Active sitei422 – 4221Cysteine radical intermediate By similarity
Active sitei424 – 4241Proton acceptor By similarity
Sitei440 – 4401Important for hydrogen atom transfer By similarity
Sitei748 – 7481Important for electron transfer By similarity
Sitei749 – 7491Important for electron transfer By similarity
Sitei774 – 7741Interacts with thioredoxin/glutaredoxin By similarity
Sitei777 – 7771Interacts with thioredoxin/glutaredoxin By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor Source: UniProtKB-EC

GO - Biological processi

  1. DNA replication Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

DNA replication

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00326.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonucleoside-diphosphate reductase large subunit (EC:1.17.4.1)
Alternative name(s):
Ribonucleotide reductase large subunit
Gene namesi
Ordered Locus Names:Mal-053
OrganismiAfrican swine fever virus (isolate Tick/Malawi/Lil 20-1/1983) (ASFV)
Taxonomic identifieri10500 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageAsfarviridaeAsfivirus
Virus hostiOrnithodoros (relapsing fever ticks) [TaxID: 6937]
Phacochoerus aethiopicus (Warthog) [TaxID: 85517]
Phacochoerus africanus (Warthog) [TaxID: 41426]
Potamochoerus larvatus (Bushpig) [TaxID: 273792]
Sus scrofa (Pig) [TaxID: 9823]

Subcellular locationi

GO - Cellular componenti

  1. ribonucleoside-diphosphate reductase complex Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 779779Ribonucleoside-diphosphate reductase large subunitPRO_0000187228Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi194 ↔ 440Redox-active By similarity

Keywords - PTMi

Disulfide bond

Expressioni

Keywords - Developmental stagei

Early protein

Interactioni

Subunit structurei

Heterotetramer composed of a homodimer of the large subunit (R1) and a homodimer of the small subunit (R2). Larger multisubunit protein complex are also active, composed of (R1)n(R2)n By similarity.

Structurei

3D structure databases

ProteinModelPortaliP26685.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni193 – 1942Substrate binding By similarity
Regioni420 – 4245Substrate binding By similarity
Regioni614 – 6185Substrate binding By similarity

Sequence similaritiesi

Family and domain databases

InterProiIPR013346. NrdE_NrdA.
IPR000788. RNR_lg_C.
IPR013509. RNR_lsu_N.
IPR008926. RNR_R1-su_N.
[Graphical view]
PfamiPF02867. Ribonuc_red_lgC. 1 hit.
PF00317. Ribonuc_red_lgN. 1 hit.
[Graphical view]
PRINTSiPR01183. RIBORDTASEM1.
SUPFAMiSSF48168. SSF48168. 1 hit.
TIGRFAMsiTIGR02506. NrdE_NrdA. 1 hit.
PROSITEiPS00089. RIBORED_LARGE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P26685-1 [UniParc]FASTAAdd to Basket

« Hide

MENFFIVKKL ASATYGKALN VDLNKLLQAL NHHSLQGLIS YCSALTILHY    50
DYSTLAARLS VYLLHQSTAS SFSKAVSLQA AQSCSRLSSH FVDVVYKYKA 100
IFDSYIDYSR DYKLSLLGIE TMKNSYLLKN KDGVIMERPQ DAYMRVAIMI 150
HGMGRVVNMK MILLTYDLLS RHVITHASPT MFNAGTKKPQ LSSCFLLNVN 200
DNLENLYDMV KTAGIISGGG GGIGLCLSGI RAKNSFISGS GLRSNGIQNY 250
IVLQNASQCY ANQGGLRPGA YAVYLELWHQ DIFTFLQMPR LKGQMAEQRL 300
NAPNLKYGLW VPDLFMEILE DQIHNRGDGT WYLFSPDQAP NLHKVFDLER 350
SQHENAHREF KKLYYQYVAE KRYTGVTTAK EIIKEWFKTV VQVGNPYIGF 400
KDAINRKSNL SHVGTITNSN LCIEITIPCW EGNEAEQGVC NLAAVNLAAF 450
IRESSYDYRG LIEAAGNVTE NLDNIIDNGY YPTEATRRSN MRHRPIGIGV 500
FGLADVFASL KMKFGSPEAI AIDEAIHAAL YYGAMRRSIE LAKEKGSHPS 550
FPGSAASKGL LQPDLWVRCG DLIPSWENRV AQTTQGVLTP KKWWQLRLAA 600
IQGVRNGYLT ALMPTATSSN STGKNECFEP FTSNLYTRRT LSGEFIILNK 650
YLMDDLEEIN LWSEDIQQQL LNAGGSIQHI LDIPAEIRER YKTSREMNQK 700
ILTKHAAARN PFVSQSMSLN YYFYEPELSQ VLTVLVLGWK KGLTTGSYYC 750
HFSPGAGTQK KIIRNSEKAC NADCEACLL 779
Length:779
Mass (Da):87,388
Last modified:August 1, 1992 - v1
Checksum:i88A3D0C8D5D10819
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M64728 Genomic DNA. Translation: AAA42732.1.
PIRiA40568. WMVZAL.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M64728 Genomic DNA. Translation: AAA42732.1 .
PIRi A40568. WMVZAL.

3D structure databases

ProteinModelPortali P26685.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

UniPathwayi UPA00326 .

Family and domain databases

InterProi IPR013346. NrdE_NrdA.
IPR000788. RNR_lg_C.
IPR013509. RNR_lsu_N.
IPR008926. RNR_R1-su_N.
[Graphical view ]
Pfami PF02867. Ribonuc_red_lgC. 1 hit.
PF00317. Ribonuc_red_lgN. 1 hit.
[Graphical view ]
PRINTSi PR01183. RIBORDTASEM1.
SUPFAMi SSF48168. SSF48168. 1 hit.
TIGRFAMsi TIGR02506. NrdE_NrdA. 1 hit.
PROSITEi PS00089. RIBORED_LARGE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The sequences of the ribonucleotide reductase genes from African swine fever virus show considerable homology with those of the orthopoxvirus, vaccinia virus."
    Boursnell M., Shaw K., Yanez R.J., Vinuela E., Dixon L.
    Virology 184:411-416(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "African swine fever virus genomes."
    Kutish G.F., Rock D.L.
    Submitted (MAR-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Entry informationi

Entry nameiRIR1_ASFM2
AccessioniPrimary (citable) accession number: P26685
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: August 1, 1992
Last modified: October 16, 2013
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Allosteric enzyme

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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