Reviewed, UniProtKB/Swiss-Prot P26676 (L_PI2HT)

Names and origin

Protein names

Recommended name:
    Large structural protein
      Short name=Protein L
Alternative name(s):
    Transcriptase
    Replicase
Including the following 3 domains:
    1- Recommended name:
            RNA-directed RNA polymerase
              EC=2.7.7.48
    2- Recommended name:
            mRNA (guanine-N(7)-)-methyltransferase
              EC=2.1.1.56
    3- Recommended name:
            mRNA guanylyltransferase
              EC=2.7.7.-

Gene names

Name:

L

Organism

Human parainfluenza 2 virus (strain Toshiba) (HPIV-2)

Taxonomic identifier

11214 [NCBI]

Taxonomic lineage

Viruses › ssRNA negative-strand viruses › Mononegavirales › Paramyxoviridae › Paramyxovirinae › Rubulavirus

Virus host

Homo sapiens (Human) [TaxID: 9606]

Protein attributes

Sequence length	2262 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Displays RNA-directed RNA polymerase, mRNA guanylyl transferase, mRNA (guanine-N(7)-)-methyltransferase and poly(A) synthetase activities. The viral mRNA guanylyl transferase displays a different biochemical reaction than the cellular enzyme. The template is composed of the viral RNA tightly encapsidated by the nucleoprotein (N). Functions either as transcriptase or as replicase. The transcriptase synthesizes subsequently the subgenomic RNAs, assuring their capping and polyadenylation by a stuttering mechanism. The transcriptase stutters on a specific sequence, resulting on a cotranscriptional editing of the phosphoprotein (P) mRNA. The replicase mode is dependent on intracellular N protein concentration. In this mode, the polymerase replicates the whole viral genome without recognizing the transcriptional signals By similarity.
Catalytic activity	Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1). S-adenosyl-L-methionine + G(5')pppR-RNA = S-adenosyl-L-homocysteine + m7G(5')pppR-RNA.
Subunit structure	Interacts with the P protein By similarity.
Subcellular location	Virion Potential. Host cytoplasm By similarity.
Sequence similarities	Belongs to the paramyxoviruses L protein family. Contains 1 RdRp catalytic domain.

Ontologies

Keywords
Biological process	RNA replication mRNA capping mRNA processing
Cellular component	Cytoplasm Virion
Ligand	ATP-binding Nucleotide-binding S-adenosyl-L-methionine
Molecular function	Methyltransferase Nucleotidyltransferase RNA-directed RNA polymerase Transferase
Technical term	Multifunctional enzyme
Gene Ontology (GO)
Biological process	mRNA capping Inferred from electronic annotation. Source: UniProtKB-KW transcription, RNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-KW host cell cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell virion Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA binding Inferred from electronic annotation. Source: InterPro RNA-directed RNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW mRNA (guanine-N7-)-methyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 2262	2262	Large structural protein		PRO_0000142734
Regions
	Domain	661 – 844	184	RdRp catalytic
	Nucleotide binding	1810 – 1819	10	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	P26676-1 [UniParc]. Last modified August 1, 1992. Version 1. Checksum: 82468303F4453B48	FASTA	2,262	256,383
	10 20 30 40 50 60 MAASSEILLP EVHLNSPIVK HKLIYYLLLG HFPHDLDISE ISPLHNNDWD QIAREESNLA 70 80 90 100 110 120 ERLGVAKSEL IKRVPAFRAT RWRSHAAVLI WPSCIPFLVK FLPHSKLQPV EQWYKLINAS 130 140 150 160 170 180 CNTISDSIDR CMENISIKLT GKNNLFSRSR GTAGAGKNSK ITLNDIQSIW ESNKWQPNVS 190 200 210 220 230 240 LWLTIKYQMR QLIMHQSSRQ PTDLVHIVDT RSGLIVITPE LVICFDRLNS VLMYFTFEMT 250 260 270 280 290 300 LMVSDMFEGR MNVTALCTIS HYLSPLGPRI DRLFSIVDEL AQLLGDTVYK VIASLESLVY 310 320 330 340 350 360 GCLQLKDPVV ELAGSFHSFI TQEIIDILIG SKALDKDESI TVTTQLLDIF SNLSPDLIAE 370 380 390 400 410 420 MLCLMRLWGH PTLTAAQVGK VRESMCAGKL LDFPTIMKTL AFFHTILING YRRKKNGMWP 430 440 450 460 470 480 PLILPKNASK SLIEFQHDNA EISYEYTLKH WKEISLIEFR KCFDFDPGEE LSIFMKDKAI 490 500 510 520 530 540 SAPRSDWMSV FRRSLIKQRH QRHHIPMPNP FNRRLLLNFL EDDSFDPVAE LRYVTGGEYL 550 560 570 580 590 600 QDDTFCASYS LKEKEIKPDG RIFAKLTNRM RSCQVIAEAI LANHAGTLMK ENGVVLNQLS 610 620 630 640 650 660 LTKSLLTMSQ IGIISEKAKR YTRDNISSQG FHTIKTDSKN KRKSKTASSY LTDPDDTFEL 670 680 690 700 710 720 SACFITTDLA KYCLQWRYQT IIHFARTLNR MYGVPHLFEW IHLRLIRSTL YVGDPFNPPA 730 740 750 760 770 780 ATDAFDLDKV LNGDIFIVSK GGIEGLCQKM WTMISISVII LSSAESKTRV MSMVQGDNQA 790 800 810 820 830 840 IAVTTRVPRS LPSIQKKELA YAASKLFFER LRANNYGLGH QLKAQETIIS STFFIYSKRV 850 860 870 880 890 900 FYQGRILTQA LKNASKLCLT ADVLGECTQA SCSNSATTIM RLTENGVEKD TCYKLNIYQS 910 920 930 940 950 960 IRQLTYDLIF PQYSIPGETI SEIFLQHPRL ISRIVLLPSQ LGGLNYLACS RLFNRNIGDP 970 980 990 1000 1010 1020 LGTAVADLKR LIKCGALESW ILYNLLARKP GKGSWATLAA DPYSLNQEYL YPPTTILKRH 1030 1040 1050 1060 1070 1080 TQNTLMEICR NPMLKGVFTD NAKEEENLLA KFLLDRDIVL PRVAHIIIDQ SSIGRKKQIQ 1090 1100 1110 1120 1130 1140 GFFDTTRTIM RRSFEIKPLS TKKTLSVIEY NTNYLSYNYP VILNPLPIPG YLNYITDQTC 1150 1160 1170 1180 1190 1200 SIDISRSLRK LSWSSLLNGR TLEGLETPDP IEVVNGFLIV GTGDCDFCMQ GDDKFTWFFL 1210 1220 1230 1240 1250 1260 PMGIIIDGNP ETNPPIRVPY IGSRTEERRV ASMAYIKGAT HSLKAALRGA GVYIWAFGDT 1270 1280 1290 1300 1310 1320 VVNWNDALDI ANTRVKISLE QLQTLTPLPT SANITHRLDD GATTLKFTPA SSYAFSSYTH 1330 1340 1350 1360 1370 1380 ISNDQQYLEI DQRVVDSNII YQQLMITGLG IIETYHNPPI RTSTQEITLH LHTSSSCCVR 1390 1400 1410 1420 1430 1440 SVDGCLICES NGEVPQITVP YTNTFVYDPD PLADYEIAHL DYLSYQAKIG STDYYSLTDK 1450 1460 1470 1480 1490 1500 IDLLAHLTAK QMINSIIGLD ETVSIVNDAV ILSDYTNNWI SECSYTKIDL VFKLMAWNFL 1510 1520 1530 1540 1550 1560 LELAFQMYYL RISSWTNIFD YTYMTLRRIP GTALNNIAAT ISHPKLLRRA MNLDIITPIH 1570 1580 1590 1600 1610 1620 APYLASLDYV KLSIDAIQWG VKQVLADLSN GIDLEILILS EDSMEISDRA MNLIARKLTL 1630 1640 1650 1660 1670 1680 LALVKGENYT FPKIKGMPPE EKCLVLTEYL AMCYQNTHHL DPDLQKYLYN LTNPKLTAFP 1690 1700 1710 1720 1730 1740 SNNFYLTRKI LNQIRESDEG QYIITSYYES FEQLETDIIL HSTLTAPYDN SENSNKVRFI 1750 1760 1770 1780 1790 1800 PFDIFPHPES LEKYPLPVDH DSQSAISTLI PGPPSHHVLR PLGVSSTAWY KGISYCRYLE 1810 1820 1830 1840 1850 1860 TQKIQTGDHL YLAEGSGASM SLLELLFPGD TVYYNSLFSS GENPPQRNYA PLPTQFVQSV 1870 1880 1890 1900 1910 1920 PYKLWQADLA DDSNLIKDFV PLWNGNGAVT DLSTKDAVAF IIHKVGAEKA SLVHIDLEST 1930 1940 1950 1960 1970 1980 ANINQQTLSR SQIHSLIIAT TVLKRGGILI YKTSWLPFSR FSQLAGLLWC FFDRIHLIRS 1990 2000 2010 2020 2030 2040 SYSDPHSHEV YLVCRLAADF RTIGFSAALV TATTLHNDGF TTIHPDVVCS YWQHHLENVG 2050 2060 2070 2080 2090 2100 RVGKVIDEIL DGLATNFFAG DNGLILRCGG TPSSRKWLEI DQLASFDLVQ DALVTLITIH 2110 2120 2130 2140 2150 2160 LKEIIEVQSS HTEDYTSLLF TPYNIGAAGK VRTIIKLILE RSLMYTVRNW LVLPSSIRDS 2170 2180 2190 2200 2210 2220 VRQDLELGSF RLMSILSEQT FLKKTPTKKY LLDQLTRTYI STFFNSHSVL PLHRPYQKQI 2230 2240 2250 2260 WKALGSVIYC SETVDIPLIK DIQIEDINDF EDIERGIDGE EL

References

[1]

"Characterizations of the human parainfluenza type 2 virus gene encoding the L protein and the intergenic sequences." Kawano M., Okamoto K., Bando H., Kondo K., Tsurudome M., Komada H., Nishio M., Ito Y. Nucleic Acids Res. 19:2739-2746(1991) [PubMed: 1645865] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	X57559 Genomic RNA. Translation: CAA40788.1.
PIR	S16664.
RefSeq	NP_598406.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	935189.
Family and domain databases
InterPro	IPR016269. RNA-dir_RNA_pol_paramyxovir. IPR014023. RNA_pol_cat. IPR001016. RNA_pol_L_viral. [Graphical view]
Pfam	PF00946. Paramyx_RNA_pol. 1 hit. [Graphical view]
PIRSF	PIRSF000830. RNA_pol_ParamyxoV. 1 hit.
PROSITE	PS50526. RDRP_SSRNA_NEG_NONSEG. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
DrugBank	DB00811. Ribavirin.

Entry information

Entry name

L_PI2HT

Accession

Primary (citable) accession number: P26676

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1992
Last sequence update:	August 1, 1992
Last modified:	June 16, 2009
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Virus (Virus annotation project)