P26674 (STE6_SCHPO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein ste6

Gene names

Name:	ste6
ORF Names:	SPCC1442.01, SPCC1450.17

Organism

Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) [Reference proteome]

Taxonomic identifier

284812 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces › Schizosaccharomyces pombe

Protein attributes

Sequence length	911 AA.
Sequence status	Complete.
Protein existence	Predicted

General annotation (Comments)

Function	Promotes the exchange of RAS-bound GDP by GTP. This protein is essential for mating.
Sequence similarities	Contains 1 N-terminal Ras-GEF domain. Contains 1 Ras-GEF domain. Contains 1 SH3 domain.

Ontologies

Keywords
Domain	SH3 domain
Molecular function	Guanine-nucleotide releasing factor
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	pheromone-dependent signal transduction involved in conjugation with cellular fusion Inferred from mutant phenotype PubMed 7975894. Source: PomBase regulation of small GTPase mediated signal transduction Inferred from electronic annotation. Source: InterPro small GTPase mediated signal transduction Inferred from electronic annotation. Source: InterPro
Cellular_component	cytosol Inferred from direct assay PubMed 16823372. Source: PomBase
Molecular_function	guanyl-nucleotide exchange factor activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 911	911	Protein ste6		PRO_0000068899
Regions
	Domain	1 – 60	60	SH3
	Domain	489 – 623	135	N-terminal Ras-GEF
	Domain	663 – 894	232	Ras-GEF

Sequences

	Sequence		Length	Mass (Da)
	P26674 [UniParc]. Last modified August 1, 1992. Version 1. Checksum: 5F8C12D20C4B753F	FASTA	911	105,186
	10 20 30 40 50 60 MRFQTTAISD YENSSNPSFL KFSAGDTIIV IEVLEDGWCD GICSEKRGWF PTSCIDSSKI 70 80 90 100 110 120 QNFFSSFHSS NEKDPNAQCC APFHVEAHLQ DSAWFEKHGV QAINSIPSSE EFLRKNLQND 130 140 150 160 170 180 IHHLVKGILT TAAAVSQSIK KEGTQVIVFG IETVRSMVLS FPLIILSTLD ENFLSEVAQV 190 200 210 220 230 240 FSSLNLLPEL SRMGCTYGEL CIRFTKLLKQ LANKFLFFFR PDVSFPSYFL GSLIAHEIHF 250 260 270 280 290 300 LPWDFNMLCS NSVQSAHTNL QPDITSFVAI LSLSHEAYHC TENEFWNLEA QKLTENTTQK 310 320 330 340 350 360 VLQLVAEDAL EAWKLDILED IDRCIQCCRR FLSANQRINY SSSENNPFSF TSQDVEALKD 370 380 390 400 410 420 ELSSNLCDLY LWSIDLEQIS PSDCLLDNYS LFVDLLVTLK VSLLRIKSII VQFSERIVFL 430 440 450 460 470 480 SLEYKFLTNI QPELNDAEKS QLDGFDLNKT NWFDSKGLVC YLMKQTSPEP LLIRNLLFSF 490 500 510 520 530 540 WSCNGKIEQD GKIKTATLVF IINYLLRTDI DSTFFTTIFL NTYASMISSS DLFSILGAHF 550 560 570 580 590 600 RFICSLNFGK ISFISHEFYR VSKRFLDILL IWFESYLVEE LDNSKSIFFL FKIYKVFEVF 610 620 630 640 650 660 VVPHFASAEE LLHSLSHLLH HPSTKRSHKM LEGKELSQEL EDLSLHNSPD PIIYKDELVL 670 680 690 700 710 720 LLPPREIAKQ LCILEFQSFS HISRIQFLTK IWDNLNRFSP KEKTSTFYLS NHLVNFVTET 730 740 750 760 770 780 IVQEEEPRRR TNVLAYFIQV CDYLRELNNF ASLFSIISAL NSSPIHRLRK TWANLNSKTL 790 800 810 820 830 840 ASFELLNNLT EARKNFSNYR DCLENCVLPC VPFLGVYFTD LTFLKTGNKD NFQNMINFDK 850 860 870 880 890 900 RTKVTRILNE IKKFQSVGYM FNPINEVQEL LNEVISRERN TNNIYQRSLT VEPRESEDQA 910 LQRLLIDSGI F

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Homologous activators of ras in fission and budding yeast." Hughes D.A., Fukui Y., Yamamoto M. Nature 344:355-357(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 972 / ATCC 24843.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X53254 Genomic DNA. Translation: CAA37345.1. CU329672 Genomic DNA. Translation: CAB40184.1.
PIR	S28098.
RefSeq	NP_588316.1. NM_001023306.2.
3D structure databases
ProteinModelPortal	P26674.
ModBase	Search...
Protein-protein interaction databases
STRING	4896.SPCC1442.01-1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	SPCC1442.01.1; SPCC1442.01.1:pep; SPCC1442.01.
GeneID	2538724.
KEGG	spo:SPCC1442.01.
Organism-specific databases
PomBase	SPCC1442.01.
Phylogenomic databases
eggNOG	NOG265981.
OrthoDB	EOG4XSPZV.
Family and domain databases
Gene3D	1.10.840.10. 1 hit.
InterPro	IPR000651. Ras-like_Gua-exchang_fac_N. IPR019804. Ras_G-nucl-exch_fac_CS. IPR008937. Ras_GEF. IPR023578. Ras_GEF_dom. IPR001895. RasGRF_CDC25. IPR001452. SH3_domain. [Graphical view]
PANTHER	PTHR23113. PTHR23113. 1 hit.
Pfam	PF00617. RasGEF. 1 hit. PF00618. RasGEF_N. 1 hit. PF00018. SH3_1. 1 hit. [Graphical view]
SMART	SM00147. RasGEF. 1 hit. SM00229. RasGEFN. 1 hit. SM00326. SH3. 1 hit. [Graphical view]
SUPFAM	SSF48366. Ras_GEF. 1 hit. SSF50044. SH3. 1 hit.
PROSITE	PS00720. RASGEF. 1 hit. PS50009. RASGEF_CAT. 1 hit. PS50212. RASGEF_NTER. 1 hit. PS50002. SH3. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	20799909.

Entry information

Entry name

STE6_SCHPO

Accession

Primary (citable) accession number: P26674
Secondary accession number(s): Q9UUM8

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1992
Last sequence update:	August 1, 1992
Last modified:	May 1, 2013
This is version 98 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program