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Protein

Surface composition regulator

Gene

glgS

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Major determinant of cell surface composition. Negatively regulates motility, adhesion and synthesis of biofilm exopolysaccharides.UniRule annotation1 Publication

GO - Biological processi

  • negative regulation of bacterial-type flagellum-dependent cell motility Source: EcoCyc
  • negative regulation of single-species biofilm formation on inanimate substrate Source: EcoCyc
  • positive regulation of cellular carbohydrate metabolic process Source: EcoCyc
Complete GO annotation...

Enzyme and pathway databases

BioCyciEcoCyc:EG11381-MONOMER.
ECOL316407:JW3021-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Surface composition regulator1 PublicationUniRule annotation
Gene namesi
Name:glgS1 Publication
Synonyms:scoR1 Publication
Ordered Locus Names:b3049, JW3021
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11381. glgS.

Pathology & Biotechi

Disruption phenotypei

Deletion mutants show increased flagella and fimbriae production, are hypermotile, and exhibit increased biofilm formation.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 6666Surface composition regulatorPRO_0000071599Add
BLAST

Proteomic databases

PaxDbiP26649.

Expressioni

Inductioni

Expression is dependent on RpoS.1 Publication

Interactioni

Protein-protein interaction databases

BioGridi4262385. 13 interactions.
IntActiP26649. 2 interactions.
STRINGi511145.b3049.

Structurei

Secondary structure

1
66
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Turni3 – 64Combined sources
Helixi7 – 2418Combined sources
Helixi30 – 367Combined sources
Helixi41 – 5919Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1RRZNMR-A1-66[»]
ProteinModelPortaliP26649.
SMRiP26649. Positions 1-66.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP26649.

Family & Domainsi

Sequence similaritiesi

Belongs to the GlgS family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105WWV. Bacteria.
ENOG411240A. LUCA.
HOGENOMiHOG000004893.
OMAiHRSWFCK.
OrthoDBiEOG6JDWHK.

Family and domain databases

Gene3Di1.20.970.20. 1 hit.
HAMAPiMF_00525. GlgS.
InterProiIPR015065. GlgS.
[Graphical view]
PfamiPF08971. GlgS. 1 hit.
[Graphical view]
ProDomiPD054490. GlgS. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF109747. SSF109747. 1 hit.

Sequencei

Sequence statusi: Complete.

P26649-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDHSLNSLNN FDFLARSFAR MHAEGRPVDI LAVTGNMDEE HRTWFCARYA
60
WYCQQMMQAR ELELEH
Length:66
Mass (Da):7,892
Last modified:August 1, 1992 - v1
Checksum:iD047AB6E7AD67523
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z11885 Genomic DNA. Translation: CAA77940.1.
U00096 Genomic DNA. Translation: AAC76085.1.
AP009048 Genomic DNA. Translation: BAA16577.1.
PIRiS25201.
RefSeqiNP_417521.1. NC_000913.3.
WP_000350095.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76085; AAC76085; b3049.
BAA16577; BAA16577; BAA16577.
GeneIDi947533.
KEGGiecj:JW3021.
eco:b3049.
PATRICi32121510. VBIEscCol129921_3142.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z11885 Genomic DNA. Translation: CAA77940.1.
U00096 Genomic DNA. Translation: AAC76085.1.
AP009048 Genomic DNA. Translation: BAA16577.1.
PIRiS25201.
RefSeqiNP_417521.1. NC_000913.3.
WP_000350095.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1RRZNMR-A1-66[»]
ProteinModelPortaliP26649.
SMRiP26649. Positions 1-66.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262385. 13 interactions.
IntActiP26649. 2 interactions.
STRINGi511145.b3049.

Proteomic databases

PaxDbiP26649.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76085; AAC76085; b3049.
BAA16577; BAA16577; BAA16577.
GeneIDi947533.
KEGGiecj:JW3021.
eco:b3049.
PATRICi32121510. VBIEscCol129921_3142.

Organism-specific databases

EchoBASEiEB1355.
EcoGeneiEG11381. glgS.

Phylogenomic databases

eggNOGiENOG4105WWV. Bacteria.
ENOG411240A. LUCA.
HOGENOMiHOG000004893.
OMAiHRSWFCK.
OrthoDBiEOG6JDWHK.

Enzyme and pathway databases

BioCyciEcoCyc:EG11381-MONOMER.
ECOL316407:JW3021-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP26649.
PROiP26649.

Family and domain databases

Gene3Di1.20.970.20. 1 hit.
HAMAPiMF_00525. GlgS.
InterProiIPR015065. GlgS.
[Graphical view]
PfamiPF08971. GlgS. 1 hit.
[Graphical view]
ProDomiPD054490. GlgS. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF109747. SSF109747. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Identification and molecular analysis of glgS, a novel growth-phase-regulated and rpoS-dependent gene involved in glycogen synthesis in Escherichia coli."
    Hengge-Aronis R., Fischer D.
    Mol. Microbiol. 6:1877-1886(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], INDUCTION.
    Strain: K12.
  2. "Construction of a contiguous 874-kb sequence of the Escherichia coli-K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features."
    Yamamoto Y., Aiba H., Baba T., Hayashi K., Inada T., Isono K., Itoh T., Kimura S., Kitagawa M., Makino K., Miki T., Mitsuhashi N., Mizobuchi K., Mori H., Nakade S., Nakamura Y., Nashimoto H., Oshima T.
    , Oyama S., Saito N., Sampei G., Satoh Y., Sivasundaram S., Tagami H., Takahashi H., Takeda J., Takemoto K., Uehara K., Wada C., Yamagata S., Horiuchi T.
    DNA Res. 4:91-113(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  5. "GlgS, described previously as a glycogen synthesis control protein, negatively regulates motility and biofilm formation in Escherichia coli."
    Rahimpour M., Montero M., Almagro G., Viale A.M., Sevilla A., Canovas M., Munoz F.J., Baroja-Fernandez E., Bahaji A., Eydallin G., Dose H., Takeuchi R., Mori H., Pozueta-Romero J.
    Biochem. J. 452:559-573(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE.
    Strain: K12 / BW25113.
  6. "1H, 15N and 13C NMR assignments, secondary structure and overall topology of the Escherichia coli GlgS protein."
    Beglova N., Fischer D., Hengge-Aronis R., Gehring K.
    Eur. J. Biochem. 246:301-310(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR.

Entry informationi

Entry nameiGLGS_ECOLI
AccessioniPrimary (citable) accession number: P26649
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: August 1, 1992
Last modified: March 16, 2016
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Caution

Was originally thought to be involved in glycogen synthesis, but it was shown later that its effect on glycogen metabolism is probably indirect.2 Publications

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.