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Protein

1-acyl-sn-glycerol-3-phosphate acyltransferase

Gene

plsC

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Converts lysophosphatidic acid (LPA) into phosphatidic acid by incorporating an acyl moiety at the 2 position. This enzyme can utilize either acyl-CoA or acyl-ACP as the fatty acyl donor.1 Publication

Catalytic activityi

Acyl-CoA + 1-acyl-sn-glycerol 3-phosphate = CoA + 1,2-diacyl-sn-glycerol 3-phosphate.
Acyl-[acyl-carrier-protein] + 1-acyl-sn-glycerol 3-phosphate = [acyl-carrier-protein] + 1,2-diacyl-sn-glycerol 3-phosphate.

Pathwayi: CDP-diacylglycerol biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes CDP-diacylglycerol from sn-glycerol 3-phosphate.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Glycerol-3-phosphate acyltransferase (plsB)
  2. 1-acyl-sn-glycerol-3-phosphate acyltransferase (plsC)
  3. Phosphatidate cytidylyltransferase (cdsA)
This subpathway is part of the pathway CDP-diacylglycerol biosynthesis, which is itself part of Phospholipid metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes CDP-diacylglycerol from sn-glycerol 3-phosphate, the pathway CDP-diacylglycerol biosynthesis and in Phospholipid metabolism.

GO - Molecular functioni

GO - Biological processi

  • CDP-diacylglycerol biosynthetic process Source: UniProtKB-UniPathway
  • glycerophospholipid biosynthetic process Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Keywords - Biological processi

Lipid biosynthesis, Lipid metabolism, Phospholipid biosynthesis, Phospholipid metabolism

Enzyme and pathway databases

BioCyciEcoCyc:1-ACYLGLYCEROL-3-P-ACYLTRANSFER-MONOMER.
ECOL316407:JW2986-MONOMER.
MetaCyc:1-ACYLGLYCEROL-3-P-ACYLTRANSFER-MONOMER.
UniPathwayiUPA00557; UER00613.

Names & Taxonomyi

Protein namesi
Recommended name:
1-acyl-sn-glycerol-3-phosphate acyltransferase (EC:2.3.1.51, EC:2.3.1.n4)
Short name:
1-AGP acyltransferase
Short name:
1-AGPAT
Alternative name(s):
Lysophosphatidic acid acyltransferase
Short name:
LPAAT
Phosphatidic acid synthase
Short name:
PA synthase
Gene namesi
Name:plsC
Synonyms:parF
Ordered Locus Names:b3018, JW2986
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11377. plsC.

Subcellular locationi

  • Cell inner membrane 1 Publication; Peripheral membrane protein 1 Publication

GO - Cellular componenti

  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002081681 – 2451-acyl-sn-glycerol-3-phosphate acyltransferaseAdd BLAST245

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-formylmethionine1 Publication1

Keywords - PTMi

Formylation

Proteomic databases

PaxDbiP26647.
PRIDEiP26647.

Interactioni

Protein-protein interaction databases

BioGridi4261779. 227 interactors.
DIPiDIP-10516N.
IntActiP26647. 1 interactor.
STRINGi511145.b3018.

Structurei

3D structure databases

ProteinModelPortaliP26647.
SMRiP26647.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi73 – 78HXXXXD motif6

Domaini

The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate.By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4107S7E. Bacteria.
COG0204. LUCA.
HOGENOMiHOG000026378.
InParanoidiP26647.
KOiK00655.
OMAiMSIISWF.
PhylomeDBiP26647.

Family and domain databases

InterProiIPR004552. AGP_acyltrans.
IPR002123. Plipid/glycerol_acylTrfase.
[Graphical view]
PfamiPF01553. Acyltransferase. 1 hit.
[Graphical view]
SMARTiSM00563. PlsC. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00530. AGP_acyltrn. 1 hit.

Sequencei

Sequence statusi: Complete.

P26647-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLYIFRLIIT VIYSILVCVF GSIYCLFSPR NPKHVATFGH MFGRLAPLFG
60 70 80 90 100
LKVECRKPTD AESYGNAIYI ANHQNNYDMV TASNIVQPPT VTVGKKSLLW
110 120 130 140 150
IPFFGQLYWL TGNLLIDRNN RTKAHGTIAE VVNHFKKRRI SIWMFPEGTR
160 170 180 190 200
SRGRGLLPFK TGAFHAAIAA GVPIIPVCVS TTSNKINLNR LHNGLVIVEM
210 220 230 240
LPPIDVSQYG KDQVRELAAH CRSIMEQKIA ELDKEVAERE AAGKV
Length:245
Mass (Da):27,453
Last modified:August 1, 1992 - v1
Checksum:iE9A1E697E7149838
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M63491 Genomic DNA. Translation: AAA24397.1.
U28377 Genomic DNA. Translation: AAA69186.1.
U00096 Genomic DNA. Translation: AAC76054.1.
AP009048 Genomic DNA. Translation: BAE77074.1.
PIRiS20460.
RefSeqiNP_417490.1. NC_000913.3.
WP_000965722.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76054; AAC76054; b3018.
BAE77074; BAE77074; BAE77074.
GeneIDi947496.
KEGGiecj:JW2986.
eco:b3018.
PATRICi32121450. VBIEscCol129921_3112.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M63491 Genomic DNA. Translation: AAA24397.1.
U28377 Genomic DNA. Translation: AAA69186.1.
U00096 Genomic DNA. Translation: AAC76054.1.
AP009048 Genomic DNA. Translation: BAE77074.1.
PIRiS20460.
RefSeqiNP_417490.1. NC_000913.3.
WP_000965722.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP26647.
SMRiP26647.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261779. 227 interactors.
DIPiDIP-10516N.
IntActiP26647. 1 interactor.
STRINGi511145.b3018.

Proteomic databases

PaxDbiP26647.
PRIDEiP26647.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76054; AAC76054; b3018.
BAE77074; BAE77074; BAE77074.
GeneIDi947496.
KEGGiecj:JW2986.
eco:b3018.
PATRICi32121450. VBIEscCol129921_3112.

Organism-specific databases

EchoBASEiEB1351.
EcoGeneiEG11377. plsC.

Phylogenomic databases

eggNOGiENOG4107S7E. Bacteria.
COG0204. LUCA.
HOGENOMiHOG000026378.
InParanoidiP26647.
KOiK00655.
OMAiMSIISWF.
PhylomeDBiP26647.

Enzyme and pathway databases

UniPathwayiUPA00557; UER00613.
BioCyciEcoCyc:1-ACYLGLYCEROL-3-P-ACYLTRANSFER-MONOMER.
ECOL316407:JW2986-MONOMER.
MetaCyc:1-ACYLGLYCEROL-3-P-ACYLTRANSFER-MONOMER.

Miscellaneous databases

PROiP26647.

Family and domain databases

InterProiIPR004552. AGP_acyltrans.
IPR002123. Plipid/glycerol_acylTrfase.
[Graphical view]
PfamiPF01553. Acyltransferase. 1 hit.
[Graphical view]
SMARTiSM00563. PlsC. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00530. AGP_acyltrn. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiPLSC_ECOLI
AccessioniPrimary (citable) accession number: P26647
Secondary accession number(s): Q2M9I2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: August 1, 1992
Last modified: November 30, 2016
This is version 122 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.