Putative quinone oxidoreductase YhdH - Escherichia coli (strain K12)

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P26646 (YHDH_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 84. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Putative quinone oxidoreductase YhdH
EC=1.6.5.-

Gene names

Name:	yhdH
Ordered Locus Names:	b3253, JW3222

Organism

Escherichia coli (strain K12)

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	324 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Subunit structure	Homodimer. Ref.4
Subcellular location	Cytoplasm Potential.
Miscellaneous	The zinc-binding residues are not conserved, suggesting this is a quinone oxidoreductase, rather than a zinc-dependent alcohol dehydrogenase.
Sequence similarities	Belongs to the zinc-containing alcohol dehydrogenase family. Quinone oxidoreductase subfamily.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	nucleotide binding Inferred from electronic annotation. Source: InterPro oxidoreductase activity Inferred from electronic annotation. Source: UniProtKB-KW zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 324

324

Putative quinone oxidoreductase YhdH

PRO_0000169493

Regions

Nucleotide binding

156 – 159

NADP

Nucleotide binding

178 – 180

NADP

Sites

Binding site

NADP; via amide nitrogen

Binding site

198

NADP

Binding site

242

NADP; via amide nitrogen

Binding site

256

NADP; via carbonyl oxygen

Binding site

267

NADP

Binding site

313

NADP

Secondary structure

324

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

P26646 [UniParc].

Last modified August 1, 1992. Version 1.
Checksum: ED1F71ECA0BDE0C1
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FASTA

324

34,724

        10         20         30         40         50         60 
MQALLLEQQD GKTLASVQTL DESRLPEGDV TVDVHWSSLN YKDALAITGK GKIIRNFPMI 

        70         80         90        100        110        120 
PGIDFAGTVR TSEDPRFHAG QEVLLTGWGV GENHWGGLAE QARVKGDWLV AMPQGLDARK 

       130        140        150        160        170        180 
AMIIGTAGFT AMLCVMALED AGVRPQDGEI VVTGASGGVG STAVALLHKL GYQVVAVSGR 

       190        200        210        220        230        240 
ESTHEYLKSL GASRVLPRDE FAESRPLEKQ VWAGAIDTVG DKVLAKVLAQ MNYGGCVAAC 

       250        260        270        280        290        300 
GLAGGFTLPT TVMPFILRNV RLQGVDSVMT PPERRAQAWQ RLVADLPESF YTQAAKEISL 

       310        320 
SEAPNFAEAI INNQIQGRTL VKVN

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The gene encoding the biotin carboxylase subunit of Escherichia coli acetyl-CoA carboxylase." Li S.-J., Cronan J.E. Jr. J. Biol. Chem. 267:855-863(1992) [PubMed: 1370469] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]	"Structure of Escherichia coli YhdH, a putative quinone oxidoreductase." Sulzenbacher G., Roig-Zamboni V., Pagot F., Grisel S., Salomoni A., Valencia C., Campanacci V., Vincentelli R., Tegoni M., Eklund H., Cambillau C. Acta Crystallogr. D 60:1855-1862(2004) [PubMed: 15388933] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.25 ANGSTROMS) IN COMPLEX WITH NADP, SUBUNIT.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M80458 Genomic DNA. Translation: AAA23407.1.
U18997 Genomic DNA. Translation: AAA58056.1.
U00096 Genomic DNA. Translation: AAC76285.1.
AP009048 Genomic DNA. Translation: BAE77295.1.

PIR

JS0688.

RefSeq

NP_417719.1. NC_000913.2.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1O89	X-ray	2.25	A	2-324	[»]
1O8C	X-ray	2.60	A/B/C/D	2-324	[»]

ProteinModelPortal

P26646.

SMR

P26646. Positions 2-324.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-12295N.

IntAct

P26646. 5 interactions.

2D gel databases

SWISS-2DPAGE

P26646.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

EBESCT00000003025; EBESCP00000003025; EBESCG00000002481.
EBESCT00000017389; EBESCP00000016680; EBESCG00000016445.

GeneID

947848.

GenomeReviews

Gene locus JW3222 in contig AP009048_GR.
Gene locus b3253 in contig U00096_GR.

KEGG

ecj:JW3222.
eco:b3253.

PATRIC

32121932. VBIEscCol129921_3350.

Organism-specific databases

EchoBASE

EB1291.

EcoGene

EG11315. yhdH.

Phylogenomic databases

eggNOG

COG0604.

GeneTree

EBGT00050000011317.

HOGENOM

HBG753318.

OMA

GEAHWGG.

PhylomeDB

P26646.

ProtClustDB

CLSK880625.

Enzyme and pathway databases

BioCyc

EcoCyc:EG11315-MONOMER.

Gene expression databases

Genevestigator

P26646.

Family and domain databases

InterPro

IPR013149. ADH_C.
IPR013154. ADH_GroES-like.
IPR002085. ADH_SF_Zn-type.
IPR011032. GroES-like.
IPR016040. NAD(P)-bd_dom.
IPR014188. Quinone_OxRdtase_YhdH.
[Graphical view]

Gene3D

G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.

PANTHER

PTHR11695. ADH_Sf_Zn. 1 hit.
PTHR11695:SF13. Quinone_oxidored_YhdH. 1 hit.

Pfam

PF08240. ADH_N. 1 hit.
PF00107. ADH_zinc_N. 1 hit.
[Graphical view]

SUPFAM

SSF50129. GroES_like. 1 hit.

TIGRFAMs

TIGR02823. Oxido_YhdH. 1 hit.

ProtoNet

Search...

Entry information

Entry name

YHDH_ECOLI

Accession

Primary (citable) accession number: P26646
Secondary accession number(s): Q2M8W1

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1992
Last sequence update:	August 1, 1992
Last modified:	January 25, 2012
This is version 84 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

2D gel databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents