P26640 (SYVC_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Valine--tRNA ligase
EC=6.1.1.9

Alternative name(s):
Protein G7a
Valyl-tRNA synthetase
Short name=ValRS

Gene names

Name:	VARS
Synonyms:	G7A, VARS2

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	1264 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	ATP + L-valine + tRNA(Val) = AMP + diphosphate + L-valyl-tRNA(Val).
Enzyme regulation	Can be regulated by protein kinase C-dependent phosphorylation.
Subunit structure	Forms high-molecular-mass aggregates with elongation factor 1.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family. Contains 1 GST C-terminal domain.
Sequence caution	The sequence CAA41990.1 differs from that shown. Reason: Frameshift at positions 620 and 640.

Ontologies

Keywords
Biological process	Protein biosynthesis
Coding sequence diversity	Polymorphism
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
PTM	Acetylation
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	regulation of translational fidelity Inferred from electronic annotation. Source: GOC tRNA aminoacylation for protein translation Traceable author statement. Source: Reactome translational elongation Non-traceable author statement. Source: UniProtKB valyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular_component	cytosol Traceable author statement. Source: Reactome mitochondrion Inferred from electronic annotation. Source: Compara
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW aminoacyl-tRNA editing activity Inferred from electronic annotation. Source: InterPro valine-tRNA ligase activity Inferred from direct assay Ref.9. Source: UniProtKB
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
EEF1A2	Q05639	1	EBI-355765,EBI-354943
EPB41	P11171	1	EBI-355765,EBI-1050906
HLA-B	P30480	1	EBI-355765,EBI-1054175
TFE3	P19532	1	EBI-355765,EBI-1048957
TRAF6	Q9Y4K3	1	EBI-355765,EBI-359276

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.8
	Chain	2 – 1264	1263	Valine--tRNA ligase		PRO_0000106253
Regions
	Domain	89 – 219	131	GST C-terminal
	Motif	344 – 354	11	"HIGH" region
	Motif	862 – 866	5	"KMSKS" region
Sites
	Binding site	865	1	ATP By similarity
Amino acid modifications
	Modified residue	2	1	N-acetylserine Ref.8
	Modified residue	645	1	N6-acetyllysine Ref.10
Natural variations
	Natural variant	51	1	P → R. Corresponds to variant rs2607015 [ dbSNP | Ensembl ].		VAR_052647
	Natural variant	51	1	P → T. Corresponds to variant rs2753960 [ dbSNP | Ensembl ].		VAR_061909
	Natural variant	181	1	R → C. Corresponds to variant rs35196751 [ dbSNP | Ensembl ].		VAR_052648
	Natural variant	626	1	P → S. Corresponds to variant rs11531 [ dbSNP | Ensembl ].		VAR_052649
	Natural variant	1008	1	P → L. Corresponds to variant rs1076827 [ dbSNP | Ensembl ].		VAR_052650
Experimental info
	Sequence conflict	51	1	P → S in CAA41990. Ref.1
	Sequence conflict	51	1	P → S in AAH12808. Ref.7
	Sequence conflict	331	1	A → G in AAA81332. Ref.9
	Sequence conflict	590	1	V → G in CAA41990. Ref.1
	Sequence conflict	792	1	S → F in CAA41990. Ref.1
	Sequence conflict	1064	1	M → I in AAA81332. Ref.9
	Sequence conflict	1169	1	Missing in AAA81332. Ref.9

Sequences

	Sequence		Length	Mass (Da)
	P26640 [UniParc]. Last modified December 1, 2000. Version 4. Checksum: 95CCDDBB3AB148AD	FASTA	1,264	140,476
	10 20 30 40 50 60 MSTLYVSPHP DAFPSLRALI AARYGEAGEG PGWGGAHPRI CLQPPPTSRT PFPPPRLPAL 70 80 90 100 110 120 EQGPGGLWVW GATAVAQLLW PAGLGGPGGS RAAVLVQQWV SYADTELIPA ACGATLPALG 130 140 150 160 170 180 LRSSAQDPQA VLGALGRALS PLEEWLRLHT YLAGEAPTLA DLAAVTALLL PFRYVLDPPA 190 200 210 220 230 240 RRIWNNVTRW FVTCVRQPEF RAVLGEVVLY SGARPLSHQP GPEAPALPKT AAQLKKEAKK 250 260 270 280 290 300 REKLEKFQQK QKIQQQQPPP GEKKPKPEKR EKRDPGVITY DLPTPPGEKK DVSGPMPDSY 310 320 330 340 350 360 SPRYVEAAWY PWWEQQGFFK PEYGRPNVSA ANPRGVFMMC IPPPNVTGSL HLGHALTNAI 370 380 390 400 410 420 QDSLTRWHRM RGETTLWNPG CDHAGIATQV VVEKKLWREQ GLSRHQLGRE AFLQEVWKWK 430 440 450 460 470 480 EEKGDRIYHQ LKKLGSSLDW DRACFTMDPK LSAAVTEAFV RLHEEGIIYR STRLVNWSCT 490 500 510 520 530 540 LNSAISDIEV DKKELTGRTL LSVPGYKEKV EFGVLVSFAY KVQGSDSDEE VVVATTRIET 550 560 570 580 590 600 MLGDVAVAVH PKDTRYQHLK GKNVIHPFLS RSLPIVFDEF VDMDFGTGAV KITPAHDQND 610 620 630 640 650 660 YEVGQRHGLE AISIMDSRGA LINVPPPFLG LPRFEARKAV LVALKERGLF RGIEDNPMVV 670 680 690 700 710 720 PLCNRSKDVV EPLLRPQWYV RCGEMAQAAS AAVTRGDLRI LPEAHQRTWH AWMDNIREWC 730 740 750 760 770 780 ISRQLWWGHR IPAYFVTVSD PAVPPGEDPD GRYWVSGRNE AEAREKAAKE FGVSPDKISL 790 800 810 820 830 840 QQDEDVLDTW FSSGLFPLSI LGWPNQSEDL SVFYPGTLLE TGHDILFFWV ARMVMLGLKL 850 860 870 880 890 900 TGRLPFREVY LHAIVRDAHG RKMSKSLGNV IDPLDVIYGI SLQGLHNQLL NSNLDPSEVE 910 920 930 940 950 960 KAKEGQKADF PAGIPECGTD ALRFGLCAYM SQGRDINLDV NRILGYRHFC NKLWNATKFA 970 980 990 1000 1010 1020 LRGLGKGFVP SPTSQPGGHE SLVDRWIRSR LTEAVRLSNQ GFQAYDFPAV TTAQYSFWLY 1030 1040 1050 1060 1070 1080 ELCDVYLECL KPVLNGVDQV AAECARQTLY TCLDVGLRLL SPFMPFVTEE LFQRLPRRMP 1090 1100 1110 1120 1130 1140 QAPPSLCVTP YPEPSECSWK DPEAEAALEL ALSITRAVRS LRADYNLTRI RPDCFLEVAD 1150 1160 1170 1180 1190 1200 EATGALASAV SGYVQALASA GVVAVLALGA PAPQGCAVAL ASDRCSIHLQ LQGLVDPARE 1210 1220 1230 1240 1250 1260 LGKLQAKRVE AQRQAQRLRE RRAASGYPVK VPLEVQEADE AKLQQTEAEL RKVDEAIALF QKML

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Evidence that gene G7a in the human major histocompatibility complex encodes valyl-tRNA synthetase." Hsieh H.-L., Campbell R.D. Biochem. J. 278:809-816(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	Erratum Hsieh S.-L., Campbell R.D. Biochem. J. 281:879-879(1992)
[3]	"Analysis of the gene-dense major histocompatibility complex class III region and its comparison to mouse." Xie T., Rowen L., Aguado B., Ahearn M.E., Madan A., Qin S., Campbell R.D., Hood L. Genome Res. 13:2621-2636(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"Homo sapiens 2,229,817bp genomic DNA of 6p21.3 HLA class I region." Shiina S., Tamiya G., Oka A., Inoko H. Submitted (SEP-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	"The DNA sequence and analysis of human chromosome 6." Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L., Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R., Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D., Andrews T.D. , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beare D.M., Beasley H., Beasley O., Bird C.P., Blakey S.E., Bray-Allen S., Brook J., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Clark S.Y., Clark G., Clee C.M., Clegg S., Cobley V., Collier R.E., Collins J.E., Colman L.K., Corby N.R., Coville G.J., Culley K.M., Dhami P., Davies J., Dunn M., Earthrowl M.E., Ellington A.E., Evans K.A., Faulkner L., Francis M.D., Frankish A., Frankland J., French L., Garner P., Garnett J., Ghori M.J., Gilby L.M., Gillson C.J., Glithero R.J., Grafham D.V., Grant M., Gribble S., Griffiths C., Griffiths M.N.D., Hall R., Halls K.S., Hammond S., Harley J.L., Hart E.A., Heath P.D., Heathcott R., Holmes S.J., Howden P.J., Howe K.L., Howell G.R., Huckle E., Humphray S.J., Humphries M.D., Hunt A.R., Johnson C.M., Joy A.A., Kay M., Keenan S.J., Kimberley A.M., King A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C.R., Lloyd D.M., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., Maslen G.L., Matthews L., McCann O.T., McLaren S.J., McLay K., McMurray A., Moore M.J.F., Mullikin J.C., Niblett D., Nickerson T., Novik K.L., Oliver K., Overton-Larty E.K., Parker A., Patel R., Pearce A.V., Peck A.I., Phillimore B.J.C.T., Phillips S., Plumb R.W., Porter K.M., Ramsey Y., Ranby S.A., Rice C.M., Ross M.T., Searle S.M., Sehra H.K., Sheridan E., Skuce C.D., Smith S., Smith M., Spraggon L., Squares S.L., Steward C.A., Sycamore N., Tamlyn-Hall G., Tester J., Theaker A.J., Thomas D.W., Thorpe A., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., White S.S., Whitehead S.L., Whittaker H., Wild A., Willey D.J., Wilmer T.E., Wood J.M., Wray P.W., Wyatt J.C., Young L., Younger R.M., Bentley D.R., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Dunham I., Rogers J., Beck S. Nature 425:805-811(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Muscle.
[8]	Bienvenut W.V., Dhillon A.S., Kolch W. Submitted (FEB-2008) to UniProtKB Cited for: PROTEIN SEQUENCE OF 2-17; 123-147; 451-461; 592-606; 619-633; 935-942; 1120-1129 AND 1252-1262, CLEAVAGE OF INITIATOR METHIONINE, ACETYLATION AT SER-2, MASS SPECTROMETRY. Tissue: Hepatoma.
[9]	"Cloning, sequencing and expression of a cDNA encoding mammalian valyl-tRNA synthetase." Vilalta A., Donovan D., Wood L., Vogeli G., Yang D.C.H. Gene 123:181-186(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 201-1263.
[10]	"Lysine acetylation targets protein complexes and co-regulates major cellular functions." Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T.C., Olsen J.V., Mann M. Science 325:834-840(2009) [PubMed] [Europe PMC] [Abstract] Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-645, MASS SPECTROMETRY.
[11]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X59303 mRNA. Translation: CAA41990.1. Frameshift. AF134726 Genomic DNA. Translation: AAD21819.1. BA000025 Genomic DNA. Translation: BAB63303.1. AL671762, AL662899 Genomic DNA. Translation: CAI18211.1. AL662899, AL671762 Genomic DNA. Translation: CAI18384.1. AL662834 Genomic DNA. Translation: CAI17732.1. CR925765 Genomic DNA. Translation: CAQ10624.1. CH471081 Genomic DNA. Translation: EAX03523.1. BC012808 mRNA. Translation: AAH12808.1. M98326 mRNA. Translation: AAA81332.1.
IPI	IPI00000873.
PIR	S17675.
RefSeq	NP_006286.1. NM_006295.2.
UniGene	Hs.520026.
3D structure databases
ProteinModelPortal	P26640.
ModBase	Search...
Protein-protein interaction databases
IntAct	P26640. 8 interactions.
MINT	MINT-1148831.
STRING	9606.ENSP00000401121.
PTM databases
PhosphoSite	P26640.
Polymorphism databases
DMDM	12644177.
Proteomic databases
PaxDb	P26640.
PRIDE	P26640.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000211402; ENSP00000211402; ENSG00000096171. ENST00000375663; ENSP00000364815; ENSG00000204394. ENST00000457796; ENSP00000403359; ENSG00000226589.
GeneID	7407.
KEGG	hsa:7407.
UCSC	uc003nxe.3. human.
Organism-specific databases
CTD	7407.
GeneCards	GC06M031745.
H-InvDB	HIX0005731. HIX0165932. HIX0166155. HIX0166435. HIX0166904. HIX0167447.
HGNC	HGNC:12651. VARS.
HPA	HPA046710.
MIM	192150. gene.
neXtProt	NX_P26640.
PharmGKB	PA37275.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG0525.
HOGENOM	HOG000020094.
HOVERGEN	HBG017878.
InParanoid	P26640.
KO	K01873.
OMA	IMDALIR.
OrthoDB	EOG4QZ7K3.
Enzyme and pathway databases
BRENDA	6.1.1.9. 2681.
Reactome	REACT_71. Gene Expression.
Gene expression databases
ArrayExpress	P26640.
Bgee	P26640.
CleanEx	HS_VARS. HS_VARS2.
Genevestigator	P26640.
GermOnline	ENSG00000204394. Homo sapiens.
Family and domain databases
Gene3D	1.20.1050.10. 1 hit. 3.40.50.620. 3 hits. 3.90.740.10. 1 hit.
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR002300. aa-tRNA-synth_Ia. IPR010987. Glutathione-S-Trfase_C-like. IPR004045. Glutathione_S-Trfase_N. IPR017933. Glutathione_S_Trfase/Cl_chnl_C. IPR004046. GST_C. IPR014729. Rossmann-like_a/b/a_fold. IPR009080. tRNAsynth_1a_anticodon-bd. IPR013155. V/L/I-tRNA-synth_anticodon-bd. IPR009008. Val/Leu/Ile-tRNA-synth_edit. IPR002303. Valyl-tRNA_ligase. [Graphical view]
PANTHER	PTHR11946:SF5. PTHR11946:SF5. 1 hit.
Pfam	PF08264. Anticodon_1. 1 hit. PF00043. GST_C. 1 hit. PF02798. GST_N. 1 hit. PF00133. tRNA-synt_1. 1 hit. [Graphical view]
PRINTS	PR00986. TRNASYNTHVAL.
SUPFAM	SSF47616. GST_C_like. 1 hit. SSF47323. tRNAsyn_1a_bind. 1 hit. SSF50677. ValRS_IleRS_edit. 1 hit.
TIGRFAMs	TIGR00422. valS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. PS50405. GST_CTER. 1 hit. [Graphical view]
ProtoNet	Search...
Other
BindingDB	P26640.
ChEMBL	CHEMBL2612.
ChiTaRS	VARS. human.
DrugBank	DB00161. L-Valine.
GenomeRNAi	7407.
NextBio	28996.
SOURCE	Search...

Entry information

Entry name

SYVC_HUMAN

Accession

Primary (citable) accession number: P26640
Secondary accession number(s): B0V1N1 , Q5JQ90, Q96E77, Q9UQM2

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1992
Last sequence update:	December 1, 2000
Last modified:	May 1, 2013
This is version 141 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.