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Protein

Valine--tRNA ligase

Gene

VARS

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + L-valine + tRNA(Val) = AMP + diphosphate + L-valyl-tRNA(Val).

Enzyme regulationi

Can be regulated by protein kinase C-dependent phosphorylation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei865ATPBy similarity1

GO - Molecular functioni

  • aminoacyl-tRNA editing activity Source: InterPro
  • ATP binding Source: UniProtKB-KW
  • valine-tRNA ligase activity Source: UniProtKB

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciZFISH:HS11461-MONOMER.
BRENDAi6.1.1.9. 2681.
ReactomeiR-HSA-379716. Cytosolic tRNA aminoacylation.

Names & Taxonomyi

Protein namesi
Recommended name:
Valine--tRNA ligase (EC:6.1.1.9)
Alternative name(s):
Protein G7a
Valyl-tRNA synthetase
Short name:
ValRS
Gene namesi
Name:VARS
Synonyms:G7A, VARS2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 6

Organism-specific databases

HGNCiHGNC:12651. VARS.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Pathology & Biotechi

Organism-specific databases

DisGeNETi7407.
OpenTargetsiENSG00000096171.
ENSG00000204394.
ENSG00000226589.
PharmGKBiPA37275.

Chemistry databases

ChEMBLiCHEMBL2612.
DrugBankiDB00161. L-Valine.

Polymorphism and mutation databases

BioMutaiVARS.
DMDMi12644177.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources1 Publication
ChainiPRO_00001062532 – 1264Valine--tRNA ligaseAdd BLAST1263

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineCombined sources1 Publication1
Modified residuei437PhosphoserineCombined sources1
Modified residuei527PhosphoserineCombined sources1
Modified residuei645N6-acetyllysineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiP26640.
MaxQBiP26640.
PaxDbiP26640.
PeptideAtlasiP26640.
PRIDEiP26640.

PTM databases

iPTMnetiP26640.
PhosphoSitePlusiP26640.
SwissPalmiP26640.

Expressioni

Gene expression databases

BgeeiENSG00000204394.
CleanExiHS_VARS.
HS_VARS2.
ExpressionAtlasiP26640. baseline and differential.
GenevisibleiP26640. HS.

Organism-specific databases

HPAiHPA046710.

Interactioni

Subunit structurei

Forms high-molecular-mass aggregates with elongation factor 1.

Binary interactionsi

WithEntry#Exp.IntActNotes
CALCOCO2Q131373EBI-355765,EBI-739580

Protein-protein interaction databases

BioGridi113250. 80 interactors.
IntActiP26640. 42 interactors.
MINTiMINT-1148831.
STRINGi9606.ENSP00000364815.

Chemistry databases

BindingDBiP26640.

Structurei

3D structure databases

ProteinModelPortaliP26640.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini89 – 219GST C-terminalAdd BLAST131

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi344 – 354"HIGH" regionAdd BLAST11
Motifi862 – 866"KMSKS" region5

Sequence similaritiesi

Contains 1 GST C-terminal domain.Curated

Phylogenomic databases

eggNOGiKOG0432. Eukaryota.
KOG0867. Eukaryota.
COG0525. LUCA.
GeneTreeiENSGT00550000074727.
HOGENOMiHOG000020094.
HOVERGENiHBG017878.
InParanoidiP26640.
KOiK01873.
OMAiSQYRFDL.
OrthoDBiEOG091G01XH.
PhylomeDBiP26640.
TreeFamiTF300648.

Family and domain databases

CDDicd07962. Anticodon_Ia_Val. 1 hit.
Gene3Di1.10.730.10. 1 hit.
1.20.1050.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02004. Val_tRNA_synth_type1. 1 hit.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR033705. Anticodon_Ia_Val.
IPR010987. Glutathione-S-Trfase_C-like.
IPR004046. GST_C.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_Ia_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR002303. Valyl-tRNA_ligase.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00043. GST_C. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00986. TRNASYNTHVAL.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF47616. SSF47616. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00422. valS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
PS50405. GST_CTER. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P26640-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSTLYVSPHP DAFPSLRALI AARYGEAGEG PGWGGAHPRI CLQPPPTSRT
60 70 80 90 100
PFPPPRLPAL EQGPGGLWVW GATAVAQLLW PAGLGGPGGS RAAVLVQQWV
110 120 130 140 150
SYADTELIPA ACGATLPALG LRSSAQDPQA VLGALGRALS PLEEWLRLHT
160 170 180 190 200
YLAGEAPTLA DLAAVTALLL PFRYVLDPPA RRIWNNVTRW FVTCVRQPEF
210 220 230 240 250
RAVLGEVVLY SGARPLSHQP GPEAPALPKT AAQLKKEAKK REKLEKFQQK
260 270 280 290 300
QKIQQQQPPP GEKKPKPEKR EKRDPGVITY DLPTPPGEKK DVSGPMPDSY
310 320 330 340 350
SPRYVEAAWY PWWEQQGFFK PEYGRPNVSA ANPRGVFMMC IPPPNVTGSL
360 370 380 390 400
HLGHALTNAI QDSLTRWHRM RGETTLWNPG CDHAGIATQV VVEKKLWREQ
410 420 430 440 450
GLSRHQLGRE AFLQEVWKWK EEKGDRIYHQ LKKLGSSLDW DRACFTMDPK
460 470 480 490 500
LSAAVTEAFV RLHEEGIIYR STRLVNWSCT LNSAISDIEV DKKELTGRTL
510 520 530 540 550
LSVPGYKEKV EFGVLVSFAY KVQGSDSDEE VVVATTRIET MLGDVAVAVH
560 570 580 590 600
PKDTRYQHLK GKNVIHPFLS RSLPIVFDEF VDMDFGTGAV KITPAHDQND
610 620 630 640 650
YEVGQRHGLE AISIMDSRGA LINVPPPFLG LPRFEARKAV LVALKERGLF
660 670 680 690 700
RGIEDNPMVV PLCNRSKDVV EPLLRPQWYV RCGEMAQAAS AAVTRGDLRI
710 720 730 740 750
LPEAHQRTWH AWMDNIREWC ISRQLWWGHR IPAYFVTVSD PAVPPGEDPD
760 770 780 790 800
GRYWVSGRNE AEAREKAAKE FGVSPDKISL QQDEDVLDTW FSSGLFPLSI
810 820 830 840 850
LGWPNQSEDL SVFYPGTLLE TGHDILFFWV ARMVMLGLKL TGRLPFREVY
860 870 880 890 900
LHAIVRDAHG RKMSKSLGNV IDPLDVIYGI SLQGLHNQLL NSNLDPSEVE
910 920 930 940 950
KAKEGQKADF PAGIPECGTD ALRFGLCAYM SQGRDINLDV NRILGYRHFC
960 970 980 990 1000
NKLWNATKFA LRGLGKGFVP SPTSQPGGHE SLVDRWIRSR LTEAVRLSNQ
1010 1020 1030 1040 1050
GFQAYDFPAV TTAQYSFWLY ELCDVYLECL KPVLNGVDQV AAECARQTLY
1060 1070 1080 1090 1100
TCLDVGLRLL SPFMPFVTEE LFQRLPRRMP QAPPSLCVTP YPEPSECSWK
1110 1120 1130 1140 1150
DPEAEAALEL ALSITRAVRS LRADYNLTRI RPDCFLEVAD EATGALASAV
1160 1170 1180 1190 1200
SGYVQALASA GVVAVLALGA PAPQGCAVAL ASDRCSIHLQ LQGLVDPARE
1210 1220 1230 1240 1250
LGKLQAKRVE AQRQAQRLRE RRAASGYPVK VPLEVQEADE AKLQQTEAEL
1260
RKVDEAIALF QKML
Length:1,264
Mass (Da):140,476
Last modified:December 1, 2000 - v4
Checksum:i95CCDDBB3AB148AD
GO
Isoform 2 (identifier: P26640-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-295: Missing.
     589-592: AVKI → PAQV
     593-1264: Missing.

Note: No experimental confirmation available.
Show »
Length:297
Mass (Da):33,795
Checksum:i67A51E4BB45D4A57
GO

Sequence cautioni

The sequence CAA41990 differs from that shown. Reason: Frameshift at positions 620 and 640.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti51P → S in CAA41990 (PubMed:1898367).Curated1
Sequence conflicti51P → S in AAH12808 (Ref. 6) Curated1
Sequence conflicti331A → G in AAA81332 (PubMed:8428657).Curated1
Sequence conflicti590V → G in CAA41990 (PubMed:1898367).Curated1
Sequence conflicti792S → F in CAA41990 (PubMed:1898367).Curated1
Sequence conflicti1064M → I in AAA81332 (PubMed:8428657).Curated1
Sequence conflicti1169Missing in AAA81332 (PubMed:8428657).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_05264751P → R.Corresponds to variant rs2607015dbSNPEnsembl.1
Natural variantiVAR_06190951P → T.Corresponds to variant rs2753960dbSNPEnsembl.1
Natural variantiVAR_052648181R → C.Corresponds to variant rs35196751dbSNPEnsembl.1
Natural variantiVAR_052649626P → S.Corresponds to variant rs11531dbSNPEnsembl.1
Natural variantiVAR_0526501008P → L.Corresponds to variant rs1076827dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0564801 – 295Missing in isoform 2. 1 PublicationAdd BLAST295
Alternative sequenceiVSP_056481589 – 592AVKI → PAQV in isoform 2. 1 Publication4
Alternative sequenceiVSP_056482593 – 1264Missing in isoform 2. 1 PublicationAdd BLAST672

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X59303 mRNA. Translation: CAA41990.1. Frameshift.
AF134726 Genomic DNA. Translation: AAD21819.1.
BA000025 Genomic DNA. Translation: BAB63303.1.
AK302762 mRNA. Translation: BAG63973.1.
AL671762, AL662899 Genomic DNA. Translation: CAI18211.1.
AL662899, AL671762 Genomic DNA. Translation: CAI18384.1.
AL662834 Genomic DNA. Translation: CAI17732.1.
CR925765 Genomic DNA. Translation: CAQ10624.1.
CH471081 Genomic DNA. Translation: EAX03523.1.
BC012808 mRNA. Translation: AAH12808.1.
M98326 mRNA. Translation: AAA81332.1.
CCDSiCCDS34412.1. [P26640-1]
PIRiS17675.
RefSeqiNP_006286.1. NM_006295.2. [P26640-1]
UniGeneiHs.520026.

Genome annotation databases

EnsembliENST00000211402; ENSP00000211402; ENSG00000096171. [P26640-1]
ENST00000375663; ENSP00000364815; ENSG00000204394. [P26640-1]
ENST00000422694; ENSP00000401121; ENSG00000224264.
ENST00000435657; ENSP00000415316; ENSG00000231116.
ENST00000457796; ENSP00000403359; ENSG00000226589. [P26640-1]
GeneIDi7407.
KEGGihsa:7407.
UCSCiuc003nxe.4. human. [P26640-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X59303 mRNA. Translation: CAA41990.1. Frameshift.
AF134726 Genomic DNA. Translation: AAD21819.1.
BA000025 Genomic DNA. Translation: BAB63303.1.
AK302762 mRNA. Translation: BAG63973.1.
AL671762, AL662899 Genomic DNA. Translation: CAI18211.1.
AL662899, AL671762 Genomic DNA. Translation: CAI18384.1.
AL662834 Genomic DNA. Translation: CAI17732.1.
CR925765 Genomic DNA. Translation: CAQ10624.1.
CH471081 Genomic DNA. Translation: EAX03523.1.
BC012808 mRNA. Translation: AAH12808.1.
M98326 mRNA. Translation: AAA81332.1.
CCDSiCCDS34412.1. [P26640-1]
PIRiS17675.
RefSeqiNP_006286.1. NM_006295.2. [P26640-1]
UniGeneiHs.520026.

3D structure databases

ProteinModelPortaliP26640.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi113250. 80 interactors.
IntActiP26640. 42 interactors.
MINTiMINT-1148831.
STRINGi9606.ENSP00000364815.

Chemistry databases

BindingDBiP26640.
ChEMBLiCHEMBL2612.
DrugBankiDB00161. L-Valine.

PTM databases

iPTMnetiP26640.
PhosphoSitePlusiP26640.
SwissPalmiP26640.

Polymorphism and mutation databases

BioMutaiVARS.
DMDMi12644177.

Proteomic databases

EPDiP26640.
MaxQBiP26640.
PaxDbiP26640.
PeptideAtlasiP26640.
PRIDEiP26640.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000211402; ENSP00000211402; ENSG00000096171. [P26640-1]
ENST00000375663; ENSP00000364815; ENSG00000204394. [P26640-1]
ENST00000422694; ENSP00000401121; ENSG00000224264.
ENST00000435657; ENSP00000415316; ENSG00000231116.
ENST00000457796; ENSP00000403359; ENSG00000226589. [P26640-1]
GeneIDi7407.
KEGGihsa:7407.
UCSCiuc003nxe.4. human. [P26640-1]

Organism-specific databases

CTDi7407.
DisGeNETi7407.
GeneCardsiVARS.
H-InvDBHIX0005731.
HIX0165932.
HIX0166155.
HIX0166435.
HIX0166904.
HIX0167447.
HGNCiHGNC:12651. VARS.
HPAiHPA046710.
MIMi192150. gene.
neXtProtiNX_P26640.
OpenTargetsiENSG00000096171.
ENSG00000204394.
ENSG00000226589.
PharmGKBiPA37275.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0432. Eukaryota.
KOG0867. Eukaryota.
COG0525. LUCA.
GeneTreeiENSGT00550000074727.
HOGENOMiHOG000020094.
HOVERGENiHBG017878.
InParanoidiP26640.
KOiK01873.
OMAiSQYRFDL.
OrthoDBiEOG091G01XH.
PhylomeDBiP26640.
TreeFamiTF300648.

Enzyme and pathway databases

BioCyciZFISH:HS11461-MONOMER.
BRENDAi6.1.1.9. 2681.
ReactomeiR-HSA-379716. Cytosolic tRNA aminoacylation.

Miscellaneous databases

ChiTaRSiVARS. human.
GeneWikiiVARS.
GenomeRNAii7407.
PROiP26640.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000204394.
CleanExiHS_VARS.
HS_VARS2.
ExpressionAtlasiP26640. baseline and differential.
GenevisibleiP26640. HS.

Family and domain databases

CDDicd07962. Anticodon_Ia_Val. 1 hit.
Gene3Di1.10.730.10. 1 hit.
1.20.1050.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02004. Val_tRNA_synth_type1. 1 hit.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR033705. Anticodon_Ia_Val.
IPR010987. Glutathione-S-Trfase_C-like.
IPR004046. GST_C.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_Ia_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR002303. Valyl-tRNA_ligase.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00043. GST_C. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00986. TRNASYNTHVAL.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF47616. SSF47616. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00422. valS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
PS50405. GST_CTER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSYVC_HUMAN
AccessioniPrimary (citable) accession number: P26640
Secondary accession number(s): B0V1N1
, B4DZ61, Q5JQ90, Q96E77, Q9UQM2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: December 1, 2000
Last modified: November 30, 2016
This is version 180 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. Human chromosome 6
    Human chromosome 6: entries, gene names and cross-references to MIM
  3. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  4. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  5. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.