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P26637

- SYLC_YEAST

UniProt

P26637 - SYLC_YEAST

Protein

Leucine--tRNA ligase, cytoplasmic

Gene

CDC60

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 131 (01 Oct 2014)
      Sequence version 1 (01 Aug 1992)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei732 – 7321ATPBy similarity

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-KW
    3. leucine-tRNA ligase activity Source: SGD

    GO - Biological processi

    1. leucyl-tRNA aminoacylation Source: SGD
    2. regulation of TOR signaling Source: SGD

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciYEAST:G3O-34056-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Leucine--tRNA ligase, cytoplasmic (EC:6.1.1.4)
    Alternative name(s):
    Leucyl-tRNA synthetase
    Short name:
    LeuRS
    Gene namesi
    Name:CDC60
    Ordered Locus Names:YPL160W
    ORF Names:P2564
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XVI

    Organism-specific databases

    CYGDiYPL160w.
    SGDiS000006081. CDC60.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: SGD

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 10901089Leucine--tRNA ligase, cytoplasmicPRO_0000152155Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylserine1 Publication
    Modified residuei142 – 1421Phosphothreonine1 Publication

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    MaxQBiP26637.
    PaxDbiP26637.
    PeptideAtlasiP26637.

    Expressioni

    Gene expression databases

    GenevestigatoriP26637.

    Interactioni

    Protein-protein interaction databases

    BioGridi36023. 52 interactions.
    DIPiDIP-6365N.
    IntActiP26637. 16 interactions.
    MINTiMINT-623412.
    STRINGi4932.YPL160W.

    Structurei

    3D structure databases

    ProteinModelPortaliP26637.
    SMRiP26637. Positions 278-525, 652-889.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi66 – 7611"HIGH" regionAdd
    BLAST
    Motifi729 – 7335"KMSKS" region

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0495.
    GeneTreeiENSGT00390000012163.
    HOGENOMiHOG000216621.
    KOiK01869.
    OMAiHAPFDYL.
    OrthoDBiEOG7TTQH2.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 3 hits.
    3.90.740.10. 1 hit.
    InterProiIPR002300. aa-tRNA-synth_Ia.
    IPR004493. Leu-tRNA-synth_Ia_arc/euk.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view]
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 2 hits.
    [Graphical view]
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 2 hits.
    TIGRFAMsiTIGR00395. leuS_arch. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P26637-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSSGLVLENT ARRDALIAIE KKYQKIWAEE HQFEIDAPSI EDEPITMDSE     50
    ELHRTYPKFM SSMAYPYMNG VMHAGHCFTL SKVEFSIGFE RMNGKRALFP 100
    LGFHCTGMPI LACADKLKRE AELFGKNFDN VPAEEEEIKE ETPAEKDHED 150
    VTKFKAKKSK AAAKKGRGKY QFEIMLQLGI PREEIIKFAD AKYWLTYFPP 200
    LCESDCTSLG ARIDWRRSFV TTDANPYYDA FIRWQMNKLK AAGKIKFGER 250
    YTIYSEKDGQ ACMDHDRQSG EGVTPQEYIG VKIEALEFAD DAAKIIDSSS 300
    DLDKSKKFYF VAATLRPETM YGQTCCFVSP TIEYGIFDAG DSYFITTERA 350
    FKNMSYQKLT PKRGFYKPIV TVPGKAFIGT KIHAPQSVYP ELRILPMETV 400
    IATKGTGVVT CVPSNSPDDY ITTKDLLHKP EYYGIKPEWI DHEIVPIMHT 450
    EKYGDLTAKA IVEEKKIQSP KDKNLLAEAK KIAYKEDYYT GTMIYGPYKG 500
    EKVEQAKNKV KADMIAAGEA FVYNEPESQV MSRSGDDCIV SLEDQWYVDY 550
    GEESWKKQAI ECLEGMQLFA PEVKNAFEGV LDWLKNWAVC RTYGLGTRLP 600
    WDEKYLVESL SDSTIYQSFY TIAHLLFKDY YGNEIGPLGI SADQMTDEVF 650
    DYIFQHQDDV KNTNIPLPAL QKLRREFEYF YPLDVSISGK DLIPNHLTFF 700
    IYTHVALFPK KFWPKGIRAN GHLMLNNSKM SKSTGNFMTL EQTVEKFGAD 750
    AARIAFADAG DTVEDANFDE SNANAAILRL FNLKEWAEEI TKESNLRTGE 800
    ITDFFDIAFE HEMNALIEKT YEQYALTNYK NALKYGLFDF QAARDYYREA 850
    SGVMHKDLIA RYIETQALLL APIAPHFAEY IYREVLGNQT SVQNAKFPRA 900
    SKPVDKGVLA ALDYLRNLQR SIREGEGQAL KKKKGKSAEI DASKPVKLTL 950
    LISESFPEWQ SQCVEIVRKL FSEQTLDDNK KVREHIEPKE MKRAMPFISL 1000
    LKQRLANEKP EDVFERELQF SEIDTVKAAA RNVKKAAQAL KIAEFSAISF 1050
    PYGAKTGKDI FTGEEVEIPP VTKIVENAVP GNPGVVFQNI 1090
    Length:1,090
    Mass (Da):124,142
    Last modified:August 1, 1992 - v1
    Checksum:i199B5A69089914C1
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X62878 Genomic DNA. Translation: CAA44671.1.
    X96770 Genomic DNA. Translation: CAA65561.1.
    Z73516 Genomic DNA. Translation: CAA97865.1.
    BK006949 Genomic DNA. Translation: DAA11274.1.
    PIRiJC1421.
    RefSeqiNP_015165.1. NM_001183974.1.

    Genome annotation databases

    EnsemblFungiiYPL160W; YPL160W; YPL160W.
    GeneIDi855943.
    KEGGisce:YPL160W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X62878 Genomic DNA. Translation: CAA44671.1 .
    X96770 Genomic DNA. Translation: CAA65561.1 .
    Z73516 Genomic DNA. Translation: CAA97865.1 .
    BK006949 Genomic DNA. Translation: DAA11274.1 .
    PIRi JC1421.
    RefSeqi NP_015165.1. NM_001183974.1.

    3D structure databases

    ProteinModelPortali P26637.
    SMRi P26637. Positions 278-525, 652-889.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 36023. 52 interactions.
    DIPi DIP-6365N.
    IntActi P26637. 16 interactions.
    MINTi MINT-623412.
    STRINGi 4932.YPL160W.

    Chemistry

    ChEMBLi CHEMBL1075253.

    Proteomic databases

    MaxQBi P26637.
    PaxDbi P26637.
    PeptideAtlasi P26637.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YPL160W ; YPL160W ; YPL160W .
    GeneIDi 855943.
    KEGGi sce:YPL160W.

    Organism-specific databases

    CYGDi YPL160w.
    SGDi S000006081. CDC60.

    Phylogenomic databases

    eggNOGi COG0495.
    GeneTreei ENSGT00390000012163.
    HOGENOMi HOG000216621.
    KOi K01869.
    OMAi HAPFDYL.
    OrthoDBi EOG7TTQH2.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-34056-MONOMER.

    Miscellaneous databases

    NextBioi 980708.
    PROi P26637.

    Gene expression databases

    Genevestigatori P26637.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 3 hits.
    3.90.740.10. 1 hit.
    InterProi IPR002300. aa-tRNA-synth_Ia.
    IPR004493. Leu-tRNA-synth_Ia_arc/euk.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view ]
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 2 hits.
    [Graphical view ]
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 2 hits.
    TIGRFAMsi TIGR00395. leuS_arch. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The cell division cycle gene CDC60 encodes cytosolic leucyl-tRNA synthetase in Saccharomyces cerevisiae."
      Hohmann S., Thevelein J.M.
      Gene 120:43-49(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "The sequence of 55 kb on the left arm of yeast chromosome XVI identifies a small nuclear RNA, a new putative protein kinase and two new putative regulators."
      Purnelle B., Coster F., Goffeau A.
      Yeast 12:1483-1492(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204511 / S288c / AB972.
    3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
      Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M.
      , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
      Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    6. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    7. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-142, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    8. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiSYLC_YEAST
    AccessioniPrimary (citable) accession number: P26637
    Secondary accession number(s): D6W3K8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1992
    Last sequence update: August 1, 1992
    Last modified: October 1, 2014
    This is version 131 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 158000 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome XVI
      Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

    External Data

    Dasty 3