Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P26637 (SYLC_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 128. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase, cytoplasmic

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:CDC60
Ordered Locus Names:YPL160W
ORF Names:P2564
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length1090 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).

Subcellular location

Cytoplasm.

Miscellaneous

Present with 158000 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.8
Chain2 – 10901089Leucine--tRNA ligase, cytoplasmic
PRO_0000152155

Regions

Motif66 – 7611"HIGH" region
Motif729 – 7335"KMSKS" region

Sites

Binding site7321ATP By similarity

Amino acid modifications

Modified residue21N-acetylserine Ref.8
Modified residue1421Phosphothreonine Ref.7

Sequences

Sequence LengthMass (Da)Tools
P26637 [UniParc].

Last modified August 1, 1992. Version 1.
Checksum: 199B5A69089914C1

FASTA1,090124,142
        10         20         30         40         50         60 
MSSGLVLENT ARRDALIAIE KKYQKIWAEE HQFEIDAPSI EDEPITMDSE ELHRTYPKFM 

        70         80         90        100        110        120 
SSMAYPYMNG VMHAGHCFTL SKVEFSIGFE RMNGKRALFP LGFHCTGMPI LACADKLKRE 

       130        140        150        160        170        180 
AELFGKNFDN VPAEEEEIKE ETPAEKDHED VTKFKAKKSK AAAKKGRGKY QFEIMLQLGI 

       190        200        210        220        230        240 
PREEIIKFAD AKYWLTYFPP LCESDCTSLG ARIDWRRSFV TTDANPYYDA FIRWQMNKLK 

       250        260        270        280        290        300 
AAGKIKFGER YTIYSEKDGQ ACMDHDRQSG EGVTPQEYIG VKIEALEFAD DAAKIIDSSS 

       310        320        330        340        350        360 
DLDKSKKFYF VAATLRPETM YGQTCCFVSP TIEYGIFDAG DSYFITTERA FKNMSYQKLT 

       370        380        390        400        410        420 
PKRGFYKPIV TVPGKAFIGT KIHAPQSVYP ELRILPMETV IATKGTGVVT CVPSNSPDDY 

       430        440        450        460        470        480 
ITTKDLLHKP EYYGIKPEWI DHEIVPIMHT EKYGDLTAKA IVEEKKIQSP KDKNLLAEAK 

       490        500        510        520        530        540 
KIAYKEDYYT GTMIYGPYKG EKVEQAKNKV KADMIAAGEA FVYNEPESQV MSRSGDDCIV 

       550        560        570        580        590        600 
SLEDQWYVDY GEESWKKQAI ECLEGMQLFA PEVKNAFEGV LDWLKNWAVC RTYGLGTRLP 

       610        620        630        640        650        660 
WDEKYLVESL SDSTIYQSFY TIAHLLFKDY YGNEIGPLGI SADQMTDEVF DYIFQHQDDV 

       670        680        690        700        710        720 
KNTNIPLPAL QKLRREFEYF YPLDVSISGK DLIPNHLTFF IYTHVALFPK KFWPKGIRAN 

       730        740        750        760        770        780 
GHLMLNNSKM SKSTGNFMTL EQTVEKFGAD AARIAFADAG DTVEDANFDE SNANAAILRL 

       790        800        810        820        830        840 
FNLKEWAEEI TKESNLRTGE ITDFFDIAFE HEMNALIEKT YEQYALTNYK NALKYGLFDF 

       850        860        870        880        890        900 
QAARDYYREA SGVMHKDLIA RYIETQALLL APIAPHFAEY IYREVLGNQT SVQNAKFPRA 

       910        920        930        940        950        960 
SKPVDKGVLA ALDYLRNLQR SIREGEGQAL KKKKGKSAEI DASKPVKLTL LISESFPEWQ 

       970        980        990       1000       1010       1020 
SQCVEIVRKL FSEQTLDDNK KVREHIEPKE MKRAMPFISL LKQRLANEKP EDVFERELQF 

      1030       1040       1050       1060       1070       1080 
SEIDTVKAAA RNVKKAAQAL KIAEFSAISF PYGAKTGKDI FTGEEVEIPP VTKIVENAVP 

      1090 
GNPGVVFQNI 

« Hide

References

« Hide 'large scale' references
[1]"The cell division cycle gene CDC60 encodes cytosolic leucyl-tRNA synthetase in Saccharomyces cerevisiae."
Hohmann S., Thevelein J.M.
Gene 120:43-49(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"The sequence of 55 kb on the left arm of yeast chromosome XVI identifies a small nuclear RNA, a new putative protein kinase and two new putative regulators."
Purnelle B., Coster F., Goffeau A.
Yeast 12:1483-1492(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204511 / S288c / AB972.
[3]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M. expand/collapse author list , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[5]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[7]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-142, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[8]"N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."
Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A., Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E., Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.
Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X62878 Genomic DNA. Translation: CAA44671.1.
X96770 Genomic DNA. Translation: CAA65561.1.
Z73516 Genomic DNA. Translation: CAA97865.1.
BK006949 Genomic DNA. Translation: DAA11274.1.
PIRJC1421.
RefSeqNP_015165.1. NM_001183974.1.

3D structure databases

ProteinModelPortalP26637.
SMRP26637. Positions 15-885.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid36023. 52 interactions.
DIPDIP-6365N.
IntActP26637. 16 interactions.
MINTMINT-623412.
STRING4932.YPL160W.

Chemistry

ChEMBLCHEMBL1075253.

Proteomic databases

PaxDbP26637.
PeptideAtlasP26637.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYPL160W; YPL160W; YPL160W.
GeneID855943.
KEGGsce:YPL160W.

Organism-specific databases

CYGDYPL160w.
SGDS000006081. CDC60.

Phylogenomic databases

eggNOGCOG0495.
GeneTreeENSGT00390000012163.
HOGENOMHOG000216621.
KOK01869.
OMAGFYEGVM.
OrthoDBEOG7TTQH2.

Enzyme and pathway databases

BioCycYEAST:G3O-34056-MONOMER.

Gene expression databases

GenevestigatorP26637.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
InterProIPR002300. aa-tRNA-synth_Ia.
IPR004493. Leu-tRNA-synth_Ia_arc/euk.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
[Graphical view]
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 2 hits.
TIGRFAMsTIGR00395. leuS_arch. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio980708.
PROP26637.

Entry information

Entry nameSYLC_YEAST
AccessionPrimary (citable) accession number: P26637
Secondary accession number(s): D6W3K8
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: August 1, 1992
Last modified: April 16, 2014
This is version 128 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XVI

Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries