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Protein

Leucine--tRNA ligase, cytoplasmic

Gene

CDC60

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Miscellaneous

Present with 158000 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei732ATPBy similarity1

GO - Molecular functioni

GO - Biological processi

  • leucyl-tRNA aminoacylation Source: SGD
  • regulation of TOR signaling Source: SGD
  • valyl-tRNA aminoacylation Source: GO_Central

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-34056-MONOMER.
BRENDAi6.1.1.4. 984.

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine--tRNA ligase, cytoplasmic (EC:6.1.1.4)
Alternative name(s):
Leucyl-tRNA synthetase
Short name:
LeuRS
Gene namesi
Name:CDC60
Ordered Locus Names:YPL160W
ORF Names:P2564
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XVI

Organism-specific databases

EuPathDBiFungiDB:YPL160W.
SGDiS000006081. CDC60.

Subcellular locationi

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL1075253.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00001521552 – 1090Leucine--tRNA ligase, cytoplasmicAdd BLAST1089

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineCombined sources1
Modified residuei142PhosphothreonineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiP26637.
PRIDEiP26637.

PTM databases

iPTMnetiP26637.

Interactioni

Protein-protein interaction databases

BioGridi36023. 238 interactors.
DIPiDIP-6365N.
IntActiP26637. 29 interactors.
MINTiMINT-623412.
STRINGi4932.YPL160W.

Chemistry databases

BindingDBiP26637.

Structurei

3D structure databases

ProteinModelPortaliP26637.
SMRiP26637.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi66 – 76"HIGH" regionAdd BLAST11
Motifi729 – 733"KMSKS" region5

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00390000012163.
HOGENOMiHOG000216621.
InParanoidiP26637.
KOiK01869.
OMAiFEYWYPV.
OrthoDBiEOG092C097K.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 1 hit.
InterProiView protein in InterPro
IPR002300. aa-tRNA-synth_Ia.
IPR004493. Leu-tRNA-synth_Ia_arc/euk.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_Ia_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
PANTHERiPTHR11946:SF99. PTHR11946:SF99. 1 hit.
PfamiView protein in Pfam
PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 2 hits.
TIGRFAMsiTIGR00395. leuS_arch. 1 hit.
PROSITEiView protein in PROSITE
PS00178. AA_TRNA_LIGASE_I. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P26637-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSGLVLENT ARRDALIAIE KKYQKIWAEE HQFEIDAPSI EDEPITMDSE
60 70 80 90 100
ELHRTYPKFM SSMAYPYMNG VMHAGHCFTL SKVEFSIGFE RMNGKRALFP
110 120 130 140 150
LGFHCTGMPI LACADKLKRE AELFGKNFDN VPAEEEEIKE ETPAEKDHED
160 170 180 190 200
VTKFKAKKSK AAAKKGRGKY QFEIMLQLGI PREEIIKFAD AKYWLTYFPP
210 220 230 240 250
LCESDCTSLG ARIDWRRSFV TTDANPYYDA FIRWQMNKLK AAGKIKFGER
260 270 280 290 300
YTIYSEKDGQ ACMDHDRQSG EGVTPQEYIG VKIEALEFAD DAAKIIDSSS
310 320 330 340 350
DLDKSKKFYF VAATLRPETM YGQTCCFVSP TIEYGIFDAG DSYFITTERA
360 370 380 390 400
FKNMSYQKLT PKRGFYKPIV TVPGKAFIGT KIHAPQSVYP ELRILPMETV
410 420 430 440 450
IATKGTGVVT CVPSNSPDDY ITTKDLLHKP EYYGIKPEWI DHEIVPIMHT
460 470 480 490 500
EKYGDLTAKA IVEEKKIQSP KDKNLLAEAK KIAYKEDYYT GTMIYGPYKG
510 520 530 540 550
EKVEQAKNKV KADMIAAGEA FVYNEPESQV MSRSGDDCIV SLEDQWYVDY
560 570 580 590 600
GEESWKKQAI ECLEGMQLFA PEVKNAFEGV LDWLKNWAVC RTYGLGTRLP
610 620 630 640 650
WDEKYLVESL SDSTIYQSFY TIAHLLFKDY YGNEIGPLGI SADQMTDEVF
660 670 680 690 700
DYIFQHQDDV KNTNIPLPAL QKLRREFEYF YPLDVSISGK DLIPNHLTFF
710 720 730 740 750
IYTHVALFPK KFWPKGIRAN GHLMLNNSKM SKSTGNFMTL EQTVEKFGAD
760 770 780 790 800
AARIAFADAG DTVEDANFDE SNANAAILRL FNLKEWAEEI TKESNLRTGE
810 820 830 840 850
ITDFFDIAFE HEMNALIEKT YEQYALTNYK NALKYGLFDF QAARDYYREA
860 870 880 890 900
SGVMHKDLIA RYIETQALLL APIAPHFAEY IYREVLGNQT SVQNAKFPRA
910 920 930 940 950
SKPVDKGVLA ALDYLRNLQR SIREGEGQAL KKKKGKSAEI DASKPVKLTL
960 970 980 990 1000
LISESFPEWQ SQCVEIVRKL FSEQTLDDNK KVREHIEPKE MKRAMPFISL
1010 1020 1030 1040 1050
LKQRLANEKP EDVFERELQF SEIDTVKAAA RNVKKAAQAL KIAEFSAISF
1060 1070 1080 1090
PYGAKTGKDI FTGEEVEIPP VTKIVENAVP GNPGVVFQNI
Length:1,090
Mass (Da):124,142
Last modified:August 1, 1992 - v1
Checksum:i199B5A69089914C1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X62878 Genomic DNA. Translation: CAA44671.1.
X96770 Genomic DNA. Translation: CAA65561.1.
Z73516 Genomic DNA. Translation: CAA97865.1.
BK006949 Genomic DNA. Translation: DAA11274.1.
PIRiJC1421.
RefSeqiNP_015165.1. NM_001183974.1.

Genome annotation databases

EnsemblFungiiYPL160W; YPL160W; YPL160W.
GeneIDi855943.
KEGGisce:YPL160W.

Similar proteinsi

Entry informationi

Entry nameiSYLC_YEAST
AccessioniPrimary (citable) accession number: P26637
Secondary accession number(s): D6W3K8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: August 1, 1992
Last modified: September 27, 2017
This is version 159 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names