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Protein

Leucine--tRNA ligase, cytoplasmic

Gene

CDC60

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei732 – 7321ATPBy similarity

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-KW
  3. leucine-tRNA ligase activity Source: SGD

GO - Biological processi

  1. leucyl-tRNA aminoacylation Source: SGD
  2. regulation of TOR signaling Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-34056-MONOMER.
BRENDAi6.1.1.4. 984.

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine--tRNA ligase, cytoplasmic (EC:6.1.1.4)
Alternative name(s):
Leucyl-tRNA synthetase
Short name:
LeuRS
Gene namesi
Name:CDC60
Ordered Locus Names:YPL160W
ORF Names:P2564
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311 Componenti: Chromosome XVI

Organism-specific databases

CYGDiYPL160w.
EuPathDBiFungiDB:YPL160W.
SGDiS000006081. CDC60.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 10901089Leucine--tRNA ligase, cytoplasmicPRO_0000152155Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserine1 Publication
Modified residuei142 – 1421Phosphothreonine1 Publication

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiP26637.
PaxDbiP26637.
PeptideAtlasiP26637.

Expressioni

Gene expression databases

GenevestigatoriP26637.

Interactioni

Protein-protein interaction databases

BioGridi36023. 52 interactions.
DIPiDIP-6365N.
IntActiP26637. 16 interactions.
MINTiMINT-623412.
STRINGi4932.YPL160W.

Structurei

3D structure databases

ProteinModelPortaliP26637.
SMRiP26637. Positions 278-525, 652-889.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi66 – 7611"HIGH" regionAdd
BLAST
Motifi729 – 7335"KMSKS" region

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0495.
GeneTreeiENSGT00390000012163.
HOGENOMiHOG000216621.
InParanoidiP26637.
KOiK01869.
OMAiNCFVEHE.
OrthoDBiEOG7TTQH2.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
InterProiIPR002300. aa-tRNA-synth_Ia.
IPR004493. Leu-tRNA-synth_Ia_arc/euk.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
[Graphical view]
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 2 hits.
TIGRFAMsiTIGR00395. leuS_arch. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P26637-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSGLVLENT ARRDALIAIE KKYQKIWAEE HQFEIDAPSI EDEPITMDSE
60 70 80 90 100
ELHRTYPKFM SSMAYPYMNG VMHAGHCFTL SKVEFSIGFE RMNGKRALFP
110 120 130 140 150
LGFHCTGMPI LACADKLKRE AELFGKNFDN VPAEEEEIKE ETPAEKDHED
160 170 180 190 200
VTKFKAKKSK AAAKKGRGKY QFEIMLQLGI PREEIIKFAD AKYWLTYFPP
210 220 230 240 250
LCESDCTSLG ARIDWRRSFV TTDANPYYDA FIRWQMNKLK AAGKIKFGER
260 270 280 290 300
YTIYSEKDGQ ACMDHDRQSG EGVTPQEYIG VKIEALEFAD DAAKIIDSSS
310 320 330 340 350
DLDKSKKFYF VAATLRPETM YGQTCCFVSP TIEYGIFDAG DSYFITTERA
360 370 380 390 400
FKNMSYQKLT PKRGFYKPIV TVPGKAFIGT KIHAPQSVYP ELRILPMETV
410 420 430 440 450
IATKGTGVVT CVPSNSPDDY ITTKDLLHKP EYYGIKPEWI DHEIVPIMHT
460 470 480 490 500
EKYGDLTAKA IVEEKKIQSP KDKNLLAEAK KIAYKEDYYT GTMIYGPYKG
510 520 530 540 550
EKVEQAKNKV KADMIAAGEA FVYNEPESQV MSRSGDDCIV SLEDQWYVDY
560 570 580 590 600
GEESWKKQAI ECLEGMQLFA PEVKNAFEGV LDWLKNWAVC RTYGLGTRLP
610 620 630 640 650
WDEKYLVESL SDSTIYQSFY TIAHLLFKDY YGNEIGPLGI SADQMTDEVF
660 670 680 690 700
DYIFQHQDDV KNTNIPLPAL QKLRREFEYF YPLDVSISGK DLIPNHLTFF
710 720 730 740 750
IYTHVALFPK KFWPKGIRAN GHLMLNNSKM SKSTGNFMTL EQTVEKFGAD
760 770 780 790 800
AARIAFADAG DTVEDANFDE SNANAAILRL FNLKEWAEEI TKESNLRTGE
810 820 830 840 850
ITDFFDIAFE HEMNALIEKT YEQYALTNYK NALKYGLFDF QAARDYYREA
860 870 880 890 900
SGVMHKDLIA RYIETQALLL APIAPHFAEY IYREVLGNQT SVQNAKFPRA
910 920 930 940 950
SKPVDKGVLA ALDYLRNLQR SIREGEGQAL KKKKGKSAEI DASKPVKLTL
960 970 980 990 1000
LISESFPEWQ SQCVEIVRKL FSEQTLDDNK KVREHIEPKE MKRAMPFISL
1010 1020 1030 1040 1050
LKQRLANEKP EDVFERELQF SEIDTVKAAA RNVKKAAQAL KIAEFSAISF
1060 1070 1080 1090
PYGAKTGKDI FTGEEVEIPP VTKIVENAVP GNPGVVFQNI
Length:1,090
Mass (Da):124,142
Last modified:August 1, 1992 - v1
Checksum:i199B5A69089914C1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X62878 Genomic DNA. Translation: CAA44671.1.
X96770 Genomic DNA. Translation: CAA65561.1.
Z73516 Genomic DNA. Translation: CAA97865.1.
BK006949 Genomic DNA. Translation: DAA11274.1.
PIRiJC1421.
RefSeqiNP_015165.1. NM_001183974.1.

Genome annotation databases

EnsemblFungiiYPL160W; YPL160W; YPL160W.
GeneIDi855943.
KEGGisce:YPL160W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X62878 Genomic DNA. Translation: CAA44671.1.
X96770 Genomic DNA. Translation: CAA65561.1.
Z73516 Genomic DNA. Translation: CAA97865.1.
BK006949 Genomic DNA. Translation: DAA11274.1.
PIRiJC1421.
RefSeqiNP_015165.1. NM_001183974.1.

3D structure databases

ProteinModelPortaliP26637.
SMRiP26637. Positions 278-525, 652-889.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36023. 52 interactions.
DIPiDIP-6365N.
IntActiP26637. 16 interactions.
MINTiMINT-623412.
STRINGi4932.YPL160W.

Chemistry

BindingDBiP26637.
ChEMBLiCHEMBL1075253.

Proteomic databases

MaxQBiP26637.
PaxDbiP26637.
PeptideAtlasiP26637.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYPL160W; YPL160W; YPL160W.
GeneIDi855943.
KEGGisce:YPL160W.

Organism-specific databases

CYGDiYPL160w.
EuPathDBiFungiDB:YPL160W.
SGDiS000006081. CDC60.

Phylogenomic databases

eggNOGiCOG0495.
GeneTreeiENSGT00390000012163.
HOGENOMiHOG000216621.
InParanoidiP26637.
KOiK01869.
OMAiNCFVEHE.
OrthoDBiEOG7TTQH2.

Enzyme and pathway databases

BioCyciYEAST:G3O-34056-MONOMER.
BRENDAi6.1.1.4. 984.

Miscellaneous databases

NextBioi980708.
PROiP26637.

Gene expression databases

GenevestigatoriP26637.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
InterProiIPR002300. aa-tRNA-synth_Ia.
IPR004493. Leu-tRNA-synth_Ia_arc/euk.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
[Graphical view]
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 2 hits.
TIGRFAMsiTIGR00395. leuS_arch. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The cell division cycle gene CDC60 encodes cytosolic leucyl-tRNA synthetase in Saccharomyces cerevisiae."
    Hohmann S., Thevelein J.M.
    Gene 120:43-49(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The sequence of 55 kb on the left arm of yeast chromosome XVI identifies a small nuclear RNA, a new putative protein kinase and two new putative regulators."
    Purnelle B., Coster F., Goffeau A.
    Yeast 12:1483-1492(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204511 / S288c / AB972.
  3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
    Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M.
    , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
    Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  6. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  7. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-142, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  8. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiSYLC_YEAST
AccessioniPrimary (citable) accession number: P26637
Secondary accession number(s): D6W3K8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: August 1, 1992
Last modified: April 29, 2015
This is version 136 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 158000 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.