P26636 (SYSC_CRIGR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Serine--tRNA ligase, cytoplasmic
EC=6.1.1.11

Alternative name(s):
Seryl-tRNA synthetase
Short name=SerRS
Seryl-tRNA(Ser/Sec) synthetase

Gene names

Name:

SARS

Organism

Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus)

Taxonomic identifier

10029 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Cricetidae › Cricetinae › Cricetulus

Protein attributes

Sequence length	199 AA.
Sequence status	Fragment.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Catalyzes the attachment of serine to tRNA(Ser). Is also able to aminoacylate tRNA(Sec) with serine, to form the misacylated tRNA L-seryl-tRNA(Sec), which will be further converted into selenocysteinyl-tRNA(Sec) By similarity.
Catalytic activity	ATP + L-serine + tRNA(Ser) = AMP + diphosphate + L-seryl-tRNA(Ser). ATP + L-serine + tRNA(Sec) = AMP + diphosphate + L-seryl-tRNA(Sec).
Pathway	Aminoacyl-tRNA biosynthesis; selenocysteinyl-tRNA(Sec) biosynthesis; L-seryl-tRNA(Sec) from L-serine and tRNA(Sec): step 1/1.
Subunit structure	Homodimer. The tRNA molecule binds across the dimer By similarity.
Subcellular location	Cytoplasm By similarity.
Domain	Consists of two distinct domains, a catalytic core and a N-terminal extension that is involved in tRNA binding By similarity.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. Type-1 seryl-tRNA synthetase subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
PTM	Acetylation
Gene Ontology (GO)
Biological process	seryl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW serine-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	‹1 – ›199	›199	Serine--tRNA ligase, cytoplasmic		PRO_0000122190
Regions
	Nucleotide binding	85 – 88	4	ATP By similarity
Sites
	Binding site	12	1	ATP; via amide nitrogen and carbonyl oxygen By similarity
	Binding site	19	1	Serine By similarity
	Binding site	123	1	Serine By similarity
Amino acid modifications
	Modified residue	17	1	N6-acetyllysine By similarity
Experimental info
	Non-terminal residue	1	1
	Non-terminal residue	199	1

Sequences

	Sequence		Length	Mass (Da)
	P26636 [UniParc]. Last modified August 1, 1992. Version 1. Checksum: 930B45C77613EA19	FASTA	199	22,813
	10 20 30 40 50 60 LHGRDTRGIF RVHQFEKIEQ FVYTSPHDNK SWEMFEEMIG TAEEFYQSLG IPYHIVNIVS 70 80 90 100 110 120 GSLNHAASKK LDLEAWFPGS GAFRELVSCS NCTDYQARRL RIRYGQTKKM MDKVEFVHML 130 140 150 160 170 180 NATMCATTRT ICAILENYQT EKGIIVPEKL REFMPPGLQE LIPFVKPAPI DQEPSKKQKK 190 QHEGSKKESK RGHPGEPAA

References

[1]	"A seryl-tRNA synthetase gene is coamplified with the adenylate deaminase 2 gene in coformycin resistant Chinese hamster fibroblasts." Lunel C., Buttin G., de Saint Vincent B.R. Nucleic Acids Res. 20:2597-2597(1992) [PubMed: 1598219] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M88136 mRNA. Translation: AAA37019.1.
PIR	S35441.
3D structure databases
ProteinModelPortal	P26636.
SMR	P26636. Positions 2-164.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR002314. aa-tRNA-synt_IIb_cons-dom. IPR006195. aa-tRNA-synth_II. IPR002317. Ser-tRNA-synth_IIa. [Graphical view]
PANTHER	PTHR11778. tRNA-synt_ser. 1 hit.
Pfam	PF00587. tRNA-synt_2b. 1 hit. [Graphical view]
PRINTS	PR00981. TRNASYNTHSER.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYSC_CRIGR

Accession

Primary (citable) accession number: P26636

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1992
Last sequence update:	August 1, 1992
Last modified:	January 25, 2012
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program