P26627 (R1AB_CVMJD) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Replicase polyprotein 1ab
Short name=pp1ab

Alternative name(s):
ORF1ab polyprotein

Cleaved into the following chain:

1. Non-structural protein 1-non-structural protein 3 fusion
   Short name=nsp1-nsp3 fusion

Gene names

Name:	rep
ORF Names:	1a-1b

Organism

Murine coronavirus (strain defective JHM) (MHV) (Murine hepatitis virus)

Taxonomic identifier

11143 [NCBI]

Taxonomic lineage

Viruses › ssRNA viruses › ssRNA positive-strand viruses, no DNA stage › Nidovirales › Coronaviridae › Coronavirinae › Betacoronavirus › Murine coronavirus › Murine hepatitis virus

Virus host

Mus musculus (Mouse) [TaxID: 10090]

Protein attributes

Sequence length	500 AA.
Sequence status	Fragment.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	The replicase polyprotein of coronaviruses is a multifunctional protein: it contains the activities necessary for the transcription of negative stranded RNA, leader RNA, subgenomic mRNAs and progeny virion RNA as well as proteinases responsible for the cleavage of the polyprotein into functional products. The papain-like proteinase 1 (PL1-PRO) is responsible for the cleavages located at the N-terminus of the replicase polyprotein. Activity of PL1-PRO is strongly dependent on zinc By similarity.
Sequence similarities	Belongs to the coronaviruses polyprotein 1ab family. Contains 1 peptidase C16 domain.
Caution	The genome of this defective strain consists of sequences derived from five discontinuous regions of the genome of the non-defective virus. Some proteins are thus truncated or absent.

Ontologies

Keywords
Domain	Zinc-finger
Ligand	Metal-binding Zinc
Molecular function	Hydrolase Protease Thiol protease
Gene Ontology (GO)
Biological_process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	RNA-directed RNA polymerase activity Inferred from electronic annotation. Source: InterPro cysteine-type peptidase activity Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – ›500	›500	Non-structural protein 1-non-structural protein 3 fusion By similarity		PRO_0000037353
Regions
	Domain	352 – 500	149	Peptidase C16 1
	Zinc finger	466 – 494	29	C4-type 1
Sites
	Active site	389	1	For PL1-PRO activity By similarity
Experimental info
	Non-terminal residue	500	1

Sequences

	Sequence		Length	Mass (Da)
	P26627 [UniParc]. Last modified August 1, 1992. Version 1. Checksum: B24DB851ED951264	FASTA	500	55,246
	10 20 30 40 50 60 MAKMGKYGLG FKWAPEFPWM LPNASEKLGN PERSEEDGFC PSAAQEPKVK GKTLVNHVRV 70 80 90 100 110 120 DCSRLPALEC CVQSAIIRDI FVDEDPQKVE ASTMMALQFG SAVLVKPSKR LSVQAWAKLG 130 140 150 160 170 180 VLPKTPAMGL FKRFCLCNTR ECVCDAHVAF QLFTVQPDGV CLGNGRFIGW FVPVTAIPEY 190 200 210 220 230 240 AKQWLQPWSI LLRKGGNKGS VTSGHSRRAV TMPVYDFNAT DVVYADENQD DDADDPVVLV 250 260 270 280 290 300 ADTQEEDGVA KEQVDSADSE ICVAHTVGQE MTEPDAVGSQ TPIASAEETE VGEACDREGI 310 320 330 340 350 360 AEVKATVCAD ALDACPDQVE AFDIEKVEDS ILSELQTELN APADKTYEDV LAFDAIYSET 370 380 390 400 410 420 LSAFYAVPSD ETHFKVCGFY SPAIERTNCW LRSTLIVMQS LPLEFKDLGM QKLWLSYKAG 430 440 450 460 470 480 YDQCFVDKLV KSAPKSIILP QGGYVADFAY FFLSQCSFKV HANWRCLKCG MELKLQGLDA 490 500 VFFYGDVVSH MCKCSFKAYF

References

[1]	"Analysis of efficiently packaged defective interfering RNAs of murine coronavirus: localization of a possible RNA-packaging signal." Makino S., Yokomori K., Lai M.M.C. J. Virol. 64:6045-6053(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	M61144 Genomic RNA. Translation: AAA46451.1.
PIR	A36388.
3D structure databases
ProteinModelPortal	P26627.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR022570. Coronavirus_NSP1. IPR002705. Pept_C30/C16_sub. IPR013016. Peptidase_C30/C16. [Graphical view]
Pfam	PF11963. DUF3477. 1 hit. PF01831. Peptidase_C16. 1 hit. [Graphical view]
PROSITE	PS51124. PEPTIDASE_C16. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

R1AB_CVMJD

Accession

Primary (citable) accession number: P26627

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1992
Last sequence update:	August 1, 1992
Last modified:	April 3, 2013
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program