Reviewed, UniProtKB/Swiss-Prot P26624 (GST28_SCHJA)

Names and origin

Protein names	Recommended name:     Glutathione S-transferase class-mu 28 kDa isozyme       Short name=GST 28     EC=2.5.1.18 Alternative name(s):     Sj28GST     Sj28 antigen
Organism	Schistosoma japonicum (Blood fluke)
Taxonomic identifier	6182 [NCBI]
Taxonomic lineage	Eukaryota › Metazoa › Platyhelminthes › Trematoda › Digenea › Strigeidida › Schistosomatoidea › Schistosomatidae › Schistosoma

Protein attributes

Sequence length	206 AA.
Sequence status	Fragment.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. GST isoenzymes appear to play a central role in the parasite detoxification system. Other functions are also suspected including a role in increasing the solubility of haematin in the parasite gut.
Catalytic activity	RX + glutathione = HX + R-S-glutathione.
Subunit structure	Homodimer.
Miscellaneous	There are at least two isoenzymes of GST in S.japonicum.
Sequence similarities	Belongs to the GST superfamily. Mu family. Contains 1 GST C-terminal domain. Contains 1 GST N-terminal domain.

Ontologies

Keywords
Molecular function	Transferase
Gene Ontology (GO)
Molecular function	glutathione transferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	‹1 – 206	›206	Glutathione S-transferase class-mu 28 kDa isozyme		PRO_0000185814
Regions
	Domain	‹1 – 81	›81	GST N-terminal
	Domain	83 – 206	124	GST C-terminal
Sites
	Active site	5	1	By similarity
Experimental info
	Non-terminal residue	1	1

Sequences

	Sequence		Length	Mass (Da)
	P26624-1 [UniParc]. Last modified August 1, 1992. Version 1. Checksum: D1AD3707EDEABAA7	FASTA	206	23,393
	10 20 30 40 50 60 VKLIYFNGRG RAEPIRMILV AAGVEFEDER IEFQDWPKIK PTIPGGRLPI VKITDKRGDV 70 80 90 100 110 120 KTMSESLAIA RFIARKHNMM GDTDDEYYII EKMIGQVEDV ESEYHKTLIK PPEEKEKISK 130 140 150 160 170 180 EILNGKVPIL LQAICETLKE STGNLTVGDK VTLADVVLIA SIDHITDLDK EFLTGKYPEI 190 200 HKHRKHLLAT SPKLAKYLSE RHATAF

References

[1]

"Comparison of the cloned genes of the 26- and 28-kilodalton glutathione S-transferases of Schistosoma japonicum and Schistosoma mansoni." Henkle K.J., Davern K.M., Wright M.D., Ramos A.J., Mitchell G.F. Mol. Biochem. Parasitol. 40:23-34(1990) [PubMed: 1693415] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Sorsogon / Philippines.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M26914 mRNA. Translation: AAA29890.1.
PIR	B44941.
3D structure databases
SMR	P26624. Positions 1-206.
ModBase	Search...
Enzyme and pathway databases
BRENDA	2.5.1.18. 1465.
Family and domain databases
InterPro	IPR010987. Glutathione-S-Trfase_C-like. IPR004045. Glutathione_S-Trfase_N. IPR017933. Glutathione_S_Trfase/Cl_chnl_C. IPR004046. GST_C. IPR012336. Thioredoxin-like_fold. IPR012335. Thioredoxin_fold. [Graphical view]
Gene3D	G3DSA:1.20.1050.10. GST_C_like. 1 hit. G3DSA:3.40.30.10. Thioredoxin_fold. 1 hit.
Pfam	PF00043. GST_C. 1 hit. PF02798. GST_N. 1 hit. [Graphical view]
PROSITE	PS50405. GST_CTER. 1 hit. PS50404. GST_NTER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GST28_SCHJA

Accession

Primary (citable) accession number: P26624

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1992
Last sequence update:	August 1, 1992
Last modified:	November 24, 2009
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)