Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

NAD-dependent malic enzyme

Gene

maeA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Miscellaneous

Can also use NADP+ but is more effective with NAD+.

Catalytic activityi

(S)-malate + NAD+ = pyruvate + CO2 + NADH.
Oxaloacetate = pyruvate + CO2.

Cofactori

Mg2+1 Publication, Mn2+1 PublicationNote: Divalent metal cations. Prefers magnesium or manganese.1 Publication

Enzyme regulationi

Non-competitively inhibited by high concentrations of NAD+ and L-malate. Also inhibited by CoA, acetyl-phosphate, palmitoyl-CoA, and oxaloacetate. Activated by aspartate.2 Publications

Kineticsi

At pH 7.2.
  1. KM=0.420 mM for L-malate2 Publications
  2. KM=0.66 mM for L-malate2 Publications
  3. KM=0.097 mM for NAD+2 Publications
  4. KM=0.0688 mM for NAD+2 Publications
  5. KM=2.59 mM for pyruvate2 Publications
  1. Vmax=125.47 µmol/min/mg enzyme2 Publications

pH dependencei

Optimum pH is 7.2 to 7.5 for L-malate.2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei104Proton donorBy similarity1
Binding sitei157NADBy similarity1
Active sitei175Proton acceptorBy similarity1
Metal bindingi246Divalent metal cationBy similarity1
Metal bindingi247Divalent metal cationBy similarity1
Metal bindingi270Divalent metal cationBy similarity1
Binding sitei270NADBy similarity1
Sitei270Important for activityBy similarity1
Binding sitei418NADBy similarity1

GO - Molecular functioni

GO - Biological processi

  • gluconeogenesis Source: EcoCyc
  • malate metabolic process Source: GO_Central
  • pyruvate metabolic process Source: GO_Central

Keywordsi

Molecular functionOxidoreductase
LigandMetal-binding, NAD

Enzyme and pathway databases

BioCyciEcoCyc:MALIC-NAD-MONOMER
MetaCyc:MALIC-NAD-MONOMER
BRENDAi1.1.1.38 2026
SABIO-RKP26616

Names & Taxonomyi

Protein namesi
Recommended name:
NAD-dependent malic enzyme (EC:1.1.1.38)
Short name:
NAD-ME
Gene namesi
Name:maeA
Synonyms:sfcA
Ordered Locus Names:b1479, JW5238
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10948 maeA

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL3286080

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001602151 – 565NAD-dependent malic enzymeAdd BLAST565

Proteomic databases

PaxDbiP26616
PRIDEiP26616

Interactioni

Subunit structurei

Homotetramer.1 Publication

Protein-protein interaction databases

BioGridi4260208, 273 interactors
IntActiP26616, 3 interactors
STRINGi316385.ECDH10B_1610

Structurei

3D structure databases

ProteinModelPortaliP26616
SMRiP26616
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the malic enzymes family.Curated

Phylogenomic databases

eggNOGiENOG4107R1I Bacteria
COG0281 LUCA
HOGENOMiHOG000042487
InParanoidiP26616
KOiK00027
OMAiKVLDYAY
PhylomeDBiP26616

Family and domain databases

Gene3Di3.40.50.10380, 1 hit
HAMAPiMF_01619 NAD_malic_enz, 1 hit
InterProiView protein in InterPro
IPR015884 Malic_enzyme_CS
IPR012301 Malic_N_dom
IPR037062 Malic_N_dom_sf
IPR012302 Malic_NAD-bd
IPR001891 Malic_OxRdtase
IPR036291 NAD(P)-bd_dom_sf
IPR023667 NAD_malic_enz_proteobac
PfamiView protein in Pfam
PF00390 malic, 1 hit
PF03949 Malic_M, 1 hit
PIRSFiPIRSF000106 ME, 1 hit
PRINTSiPR00072 MALOXRDTASE
SMARTiView protein in SMART
SM01274 malic, 1 hit
SM00919 Malic_M, 1 hit
SUPFAMiSSF51735 SSF51735, 1 hit
PROSITEiView protein in PROSITE
PS00331 MALIC_ENZYMES, 1 hit

Sequencei

Sequence statusi: Complete.

P26616-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEPKTKKQRS LYIPYAGPVL LEFPLLNKGS AFSMEERRNF NLLGLLPEVV
60 70 80 90 100
ETIEEQAERA WIQYQGFKTE IDKHIYLRNI QDTNETLFYR LVNNHLDEMM
110 120 130 140 150
PVIYTPTVGA ACERFSEIYR RSRGVFISYQ NRHNMDDILQ NVPNHNIKVI
160 170 180 190 200
VVTDGERILG LGDQGIGGMG IPIGKLSLYT ACGGISPAYT LPVVLDVGTN
210 220 230 240 250
NQQLLNDPLY MGWRNPRITD DEYYEFVDEF IQAVKQRWPD VLLQFEDFAQ
260 270 280 290 300
KNAMPLLNRY RNEICSFNDD IQGTAAVTVG TLIAASRAAG GQLSEKKIVF
310 320 330 340 350
LGAGSAGCGI AEMIISQTQR EGLSEEAARQ KVFMVDRFGL LTDKMPNLLP
360 370 380 390 400
FQTKLVQKRE NLSDWDTDSD VLSLLDVVRN VKPDILIGVS GQTGLFTEEI
410 420 430 440 450
IREMHKHCPR PIVMPLSNPT SRVEATPQDI IAWTEGNALV ATGSPFNPVV
460 470 480 490 500
WKDKIYPIAQ CNNAFIFPGI GLGVIASGAS RITDEMLMSA SETLAQYSPL
510 520 530 540 550
VLNGEGMVLP ELKDIQKVSR AIAFAVGKMA QQQGVAVKTS AEALQQAIDD
560
NFWQAEYRDY RRTSI
Length:565
Mass (Da):63,197
Last modified:December 6, 2005 - v4
Checksum:iFFD61212F709EE3A
GO

Sequence cautioni

The sequence CAA39419 differs from that shown. The sequence differs from position 433 onward for unknown reasons.Curated
The sequence CAA39419 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA Translation: AAC74552.2
AP009048 Genomic DNA Translation: BAA15127.2
X55956 Genomic DNA Translation: CAA39419.1 Sequence problems.
PIRiB64901
RefSeqiNP_415996.2, NC_000913.3
WP_000433476.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC74552; AAC74552; b1479
BAA15127; BAA15127; BAA15127
GeneIDi946031
KEGGiecj:JW5238
eco:b1479
PATRICifig|1411691.4.peg.788

Similar proteinsi

Entry informationi

Entry nameiMAO1_ECOLI
AccessioniPrimary (citable) accession number: P26616
Secondary accession number(s): P78224
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: December 6, 2005
Last modified: March 28, 2018
This is version 146 of the entry and version 4 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Cookie policy

We would like to use anonymized google analytics cookies to gather statistics on how uniprot.org is used in aggregate. Learn more

UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health