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P26616

- MAO1_ECOLI

UniProt

P26616 - MAO1_ECOLI

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Protein

NAD-dependent malic enzyme

Gene

maeA

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Catalytic activityi

(S)-malate + NAD+ = pyruvate + CO2 + NADH.
Oxaloacetate = pyruvate + CO2.

Cofactori

Mg2+1 Publication, Mn2+1 PublicationNote: Divalent metal cations. Prefers magnesium or manganese.1 Publication

Enzyme regulationi

Non-competitively inhibited by high concentrations of NAD+ and L-malate. Also inhibited by CoA, acetyl-phosphate, palmitoyl-CoA, and oxaloacetate. Activated by aspartate.2 Publications

Kineticsi

At pH 7.2.

  1. KM=0.420 mM for L-malate2 Publications
  2. KM=0.66 mM for L-malate2 Publications
  3. KM=0.097 mM for NAD+2 Publications
  4. KM=0.0688 mM for NAD+2 Publications
  5. KM=2.59 mM for pyruvate2 Publications

Vmax=125.47 µmol/min/mg enzyme2 Publications

pH dependencei

Optimum pH is 7.2 to 7.5 for L-malate.2 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei104 – 1041Proton donorBy similarity
Binding sitei157 – 1571NADBy similarity
Active sitei175 – 1751Proton acceptorBy similarity
Metal bindingi246 – 2461Divalent metal cationBy similarity
Metal bindingi247 – 2471Divalent metal cationBy similarity
Metal bindingi270 – 2701Divalent metal cationBy similarity
Binding sitei270 – 2701NADBy similarity
Sitei270 – 2701Important for activityBy similarity
Binding sitei418 – 4181NADBy similarity

GO - Molecular functioni

  1. malate dehydrogenase (decarboxylating) (NAD+) activity Source: EcoCyc
  2. metal ion binding Source: UniProtKB-KW
  3. NAD binding Source: InterPro
  4. oxaloacetate decarboxylase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. gluconeogenesis Source: EcoCyc
  2. malate metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

Metal-binding, NAD

Enzyme and pathway databases

BioCyciEcoCyc:MALIC-NAD-MONOMER.
ECOL316407:JW5238-MONOMER.
MetaCyc:MALIC-NAD-MONOMER.
SABIO-RKP26616.

Names & Taxonomyi

Protein namesi
Recommended name:
NAD-dependent malic enzyme (EC:1.1.1.38)
Short name:
NAD-ME
Gene namesi
Name:maeA
Synonyms:sfcA
Ordered Locus Names:b1479, JW5238
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

Organism-specific databases

EcoGeneiEG10948. maeA.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 565565NAD-dependent malic enzymePRO_0000160215Add
BLAST

Proteomic databases

PaxDbiP26616.
PRIDEiP26616.

Expressioni

Gene expression databases

GenevestigatoriP26616.

Interactioni

Subunit structurei

Homotetramer.1 Publication

Protein-protein interaction databases

IntActiP26616. 3 interactions.
STRINGi511145.b1479.

Structurei

3D structure databases

ProteinModelPortaliP26616.
SMRiP26616. Positions 17-536.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the malic enzymes family.Curated

Phylogenomic databases

eggNOGiCOG0281.
HOGENOMiHOG000042487.
InParanoidiP26616.
KOiK00027.
OMAiIQDNNET.
OrthoDBiEOG6QVRGM.
PhylomeDBiP26616.

Family and domain databases

Gene3Di3.40.50.10380. 1 hit.
3.40.50.720. 1 hit.
HAMAPiMF_01619. NAD_malic_enz.
InterProiIPR015884. Malic_enzyme_CS.
IPR012301. Malic_N_dom.
IPR012302. Malic_NAD-bd.
IPR001891. Malic_OxRdtase.
IPR016040. NAD(P)-bd_dom.
IPR023667. NAD_malic_enz_proteobac.
[Graphical view]
PfamiPF00390. malic. 1 hit.
PF03949. Malic_M. 1 hit.
[Graphical view]
PIRSFiPIRSF000106. ME. 1 hit.
PRINTSiPR00072. MALOXRDTASE.
SMARTiSM00919. Malic_M. 1 hit.
[Graphical view]
PROSITEiPS00331. MALIC_ENZYMES. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P26616-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MEPKTKKQRS LYIPYAGPVL LEFPLLNKGS AFSMEERRNF NLLGLLPEVV
60 70 80 90 100
ETIEEQAERA WIQYQGFKTE IDKHIYLRNI QDTNETLFYR LVNNHLDEMM
110 120 130 140 150
PVIYTPTVGA ACERFSEIYR RSRGVFISYQ NRHNMDDILQ NVPNHNIKVI
160 170 180 190 200
VVTDGERILG LGDQGIGGMG IPIGKLSLYT ACGGISPAYT LPVVLDVGTN
210 220 230 240 250
NQQLLNDPLY MGWRNPRITD DEYYEFVDEF IQAVKQRWPD VLLQFEDFAQ
260 270 280 290 300
KNAMPLLNRY RNEICSFNDD IQGTAAVTVG TLIAASRAAG GQLSEKKIVF
310 320 330 340 350
LGAGSAGCGI AEMIISQTQR EGLSEEAARQ KVFMVDRFGL LTDKMPNLLP
360 370 380 390 400
FQTKLVQKRE NLSDWDTDSD VLSLLDVVRN VKPDILIGVS GQTGLFTEEI
410 420 430 440 450
IREMHKHCPR PIVMPLSNPT SRVEATPQDI IAWTEGNALV ATGSPFNPVV
460 470 480 490 500
WKDKIYPIAQ CNNAFIFPGI GLGVIASGAS RITDEMLMSA SETLAQYSPL
510 520 530 540 550
VLNGEGMVLP ELKDIQKVSR AIAFAVGKMA QQQGVAVKTS AEALQQAIDD
560
NFWQAEYRDY RRTSI
Length:565
Mass (Da):63,197
Last modified:December 6, 2005 - v4
Checksum:iFFD61212F709EE3A
GO

Sequence cautioni

The sequence CAA39419.1 differs from that shown. Reason: The sequence differs from position 433 onward for unknown reasons.Curated
The sequence CAA39419.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC74552.2.
AP009048 Genomic DNA. Translation: BAA15127.2.
X55956 Genomic DNA. Translation: CAA39419.1. Sequence problems.
PIRiB64901.
RefSeqiNP_415996.2. NC_000913.3.
YP_489744.1. NC_007779.1.

Genome annotation databases

EnsemblBacteriaiAAC74552; AAC74552; b1479.
BAA15127; BAA15127; BAA15127.
GeneIDi12931214.
946031.
KEGGiecj:Y75_p1455.
eco:b1479.
PATRICi32118252. VBIEscCol129921_1546.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC74552.2 .
AP009048 Genomic DNA. Translation: BAA15127.2 .
X55956 Genomic DNA. Translation: CAA39419.1 . Sequence problems.
PIRi B64901.
RefSeqi NP_415996.2. NC_000913.3.
YP_489744.1. NC_007779.1.

3D structure databases

ProteinModelPortali P26616.
SMRi P26616. Positions 17-536.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi P26616. 3 interactions.
STRINGi 511145.b1479.

Proteomic databases

PaxDbi P26616.
PRIDEi P26616.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC74552 ; AAC74552 ; b1479 .
BAA15127 ; BAA15127 ; BAA15127 .
GeneIDi 12931214.
946031.
KEGGi ecj:Y75_p1455.
eco:b1479.
PATRICi 32118252. VBIEscCol129921_1546.

Organism-specific databases

EchoBASEi EB0941.
EcoGenei EG10948. maeA.

Phylogenomic databases

eggNOGi COG0281.
HOGENOMi HOG000042487.
InParanoidi P26616.
KOi K00027.
OMAi IQDNNET.
OrthoDBi EOG6QVRGM.
PhylomeDBi P26616.

Enzyme and pathway databases

BioCyci EcoCyc:MALIC-NAD-MONOMER.
ECOL316407:JW5238-MONOMER.
MetaCyc:MALIC-NAD-MONOMER.
SABIO-RK P26616.

Miscellaneous databases

PROi P26616.

Gene expression databases

Genevestigatori P26616.

Family and domain databases

Gene3Di 3.40.50.10380. 1 hit.
3.40.50.720. 1 hit.
HAMAPi MF_01619. NAD_malic_enz.
InterProi IPR015884. Malic_enzyme_CS.
IPR012301. Malic_N_dom.
IPR012302. Malic_NAD-bd.
IPR001891. Malic_OxRdtase.
IPR016040. NAD(P)-bd_dom.
IPR023667. NAD_malic_enz_proteobac.
[Graphical view ]
Pfami PF00390. malic. 1 hit.
PF03949. Malic_M. 1 hit.
[Graphical view ]
PIRSFi PIRSF000106. ME. 1 hit.
PRINTSi PR00072. MALOXRDTASE.
SMARTi SM00919. Malic_M. 1 hit.
[Graphical view ]
PROSITEi PS00331. MALIC_ENZYMES. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  4. "Physical analysis of spontaneous and mutagen-induced mutants of Escherichia coli K-12 expressing DNA exonuclease VIII activity."
    Mahajan S.K., Chu C.C., Willis D.K., Templin A., Clark A.J.
    Genetics 125:261-273(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-431.
    Strain: K12.
  5. "Escherichia coli malic enzymes: two isoforms with substantial differences in kinetic properties, metabolic regulation, and structure."
    Bologna F.P., Andreo C.S., Drincovich M.F.
    J. Bacteriol. 189:5937-5946(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: BIOPHYSICOCHEMICAL PROPERTIES, COFACTOR, ENZYME REGULATION, SUBUNIT.
    Strain: K12.
  6. "Over-expression, purification, and characterization of recombinant NAD-malic enzyme from Escherichia coli K12."
    Wang J., Tan H., Zhao Z.K.
    Protein Expr. Purif. 53:97-103(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: BIOPHYSICOCHEMICAL PROPERTIES, ENZYME REGULATION.
    Strain: K12 / MG1655 / ATCC 47076.

Entry informationi

Entry nameiMAO1_ECOLI
AccessioniPrimary (citable) accession number: P26616
Secondary accession number(s): P78224
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: December 6, 2005
Last modified: November 26, 2014
This is version 123 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Can also use NADP+ but is more effective with NAD+.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3