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P26616

- MAO1_ECOLI

UniProt

P26616 - MAO1_ECOLI

Protein

NAD-dependent malic enzyme

Gene

maeA

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 121 (01 Oct 2014)
      Sequence version 4 (06 Dec 2005)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    (S)-malate + NAD+ = pyruvate + CO2 + NADH.
    Oxaloacetate = pyruvate + CO2.

    Cofactori

    Divalent metal cations. Prefers magnesium or manganese.1 Publication

    Enzyme regulationi

    Non-competitively inhibited by high concentrations of NAD+ and L-malate. Also inhibited by CoA, acetyl-phosphate, palmitoyl-CoA, and oxaloacetate. Activated by aspartate.2 Publications

    Kineticsi

    At pH 7.2.

    1. KM=0.420 mM for L-malate2 Publications
    2. KM=0.66 mM for L-malate2 Publications
    3. KM=0.097 mM for NAD+2 Publications
    4. KM=0.0688 mM for NAD+2 Publications
    5. KM=2.59 mM for pyruvate2 Publications

    Vmax=125.47 µmol/min/mg enzyme2 Publications

    pH dependencei

    Optimum pH is 7.2 to 7.5 for L-malate.2 Publications

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei104 – 1041Proton donorBy similarity
    Binding sitei157 – 1571NADBy similarity
    Active sitei175 – 1751Proton acceptorBy similarity
    Metal bindingi246 – 2461Divalent metal cationBy similarity
    Metal bindingi247 – 2471Divalent metal cationBy similarity
    Metal bindingi270 – 2701Divalent metal cationBy similarity
    Binding sitei270 – 2701NADBy similarity
    Sitei270 – 2701Important for activityBy similarity
    Binding sitei418 – 4181NADBy similarity

    GO - Molecular functioni

    1. malate dehydrogenase (decarboxylating) (NAD+) activity Source: EcoCyc
    2. metal ion binding Source: UniProtKB-KW
    3. NAD binding Source: InterPro
    4. oxaloacetate decarboxylase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. gluconeogenesis Source: EcoCyc
    2. malate metabolic process Source: InterPro

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Ligandi

    Metal-binding, NAD

    Enzyme and pathway databases

    BioCyciEcoCyc:MALIC-NAD-MONOMER.
    ECOL316407:JW5238-MONOMER.
    MetaCyc:MALIC-NAD-MONOMER.
    SABIO-RKP26616.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    NAD-dependent malic enzyme (EC:1.1.1.38)
    Short name:
    NAD-ME
    Gene namesi
    Name:maeA
    Synonyms:sfcA
    Ordered Locus Names:b1479, JW5238
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

    Organism-specific databases

    EcoGeneiEG10948. maeA.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 565565NAD-dependent malic enzymePRO_0000160215Add
    BLAST

    Proteomic databases

    PaxDbiP26616.
    PRIDEiP26616.

    Expressioni

    Gene expression databases

    GenevestigatoriP26616.

    Interactioni

    Subunit structurei

    Homotetramer.1 Publication

    Protein-protein interaction databases

    IntActiP26616. 3 interactions.
    STRINGi511145.b1479.

    Structurei

    3D structure databases

    ProteinModelPortaliP26616.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the malic enzymes family.Curated

    Phylogenomic databases

    eggNOGiCOG0281.
    HOGENOMiHOG000042487.
    KOiK00027.
    OMAiIQDNNET.
    OrthoDBiEOG6QVRGM.
    PhylomeDBiP26616.

    Family and domain databases

    Gene3Di3.40.50.10380. 1 hit.
    3.40.50.720. 1 hit.
    HAMAPiMF_01619. NAD_malic_enz.
    InterProiIPR015884. Malic_enzyme_CS.
    IPR012301. Malic_N_dom.
    IPR012302. Malic_NAD-bd.
    IPR001891. Malic_OxRdtase.
    IPR016040. NAD(P)-bd_dom.
    IPR023667. NAD_malic_enz_proteobac.
    [Graphical view]
    PfamiPF00390. malic. 1 hit.
    PF03949. Malic_M. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000106. ME. 1 hit.
    PRINTSiPR00072. MALOXRDTASE.
    SMARTiSM00919. Malic_M. 1 hit.
    [Graphical view]
    PROSITEiPS00331. MALIC_ENZYMES. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P26616-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEPKTKKQRS LYIPYAGPVL LEFPLLNKGS AFSMEERRNF NLLGLLPEVV    50
    ETIEEQAERA WIQYQGFKTE IDKHIYLRNI QDTNETLFYR LVNNHLDEMM 100
    PVIYTPTVGA ACERFSEIYR RSRGVFISYQ NRHNMDDILQ NVPNHNIKVI 150
    VVTDGERILG LGDQGIGGMG IPIGKLSLYT ACGGISPAYT LPVVLDVGTN 200
    NQQLLNDPLY MGWRNPRITD DEYYEFVDEF IQAVKQRWPD VLLQFEDFAQ 250
    KNAMPLLNRY RNEICSFNDD IQGTAAVTVG TLIAASRAAG GQLSEKKIVF 300
    LGAGSAGCGI AEMIISQTQR EGLSEEAARQ KVFMVDRFGL LTDKMPNLLP 350
    FQTKLVQKRE NLSDWDTDSD VLSLLDVVRN VKPDILIGVS GQTGLFTEEI 400
    IREMHKHCPR PIVMPLSNPT SRVEATPQDI IAWTEGNALV ATGSPFNPVV 450
    WKDKIYPIAQ CNNAFIFPGI GLGVIASGAS RITDEMLMSA SETLAQYSPL 500
    VLNGEGMVLP ELKDIQKVSR AIAFAVGKMA QQQGVAVKTS AEALQQAIDD 550
    NFWQAEYRDY RRTSI 565
    Length:565
    Mass (Da):63,197
    Last modified:December 6, 2005 - v4
    Checksum:iFFD61212F709EE3A
    GO

    Sequence cautioni

    The sequence CAA39419.1 differs from that shown. Reason: The sequence differs from position 433 onward for unknown reasons.
    The sequence CAA39419.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U00096 Genomic DNA. Translation: AAC74552.2.
    AP009048 Genomic DNA. Translation: BAA15127.2.
    X55956 Genomic DNA. Translation: CAA39419.1. Sequence problems.
    PIRiB64901.
    RefSeqiNP_415996.2. NC_000913.3.
    YP_489744.1. NC_007779.1.

    Genome annotation databases

    EnsemblBacteriaiAAC74552; AAC74552; b1479.
    BAA15127; BAA15127; BAA15127.
    GeneIDi12931214.
    946031.
    KEGGiecj:Y75_p1455.
    eco:b1479.
    PATRICi32118252. VBIEscCol129921_1546.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U00096 Genomic DNA. Translation: AAC74552.2 .
    AP009048 Genomic DNA. Translation: BAA15127.2 .
    X55956 Genomic DNA. Translation: CAA39419.1 . Sequence problems.
    PIRi B64901.
    RefSeqi NP_415996.2. NC_000913.3.
    YP_489744.1. NC_007779.1.

    3D structure databases

    ProteinModelPortali P26616.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P26616. 3 interactions.
    STRINGi 511145.b1479.

    Proteomic databases

    PaxDbi P26616.
    PRIDEi P26616.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC74552 ; AAC74552 ; b1479 .
    BAA15127 ; BAA15127 ; BAA15127 .
    GeneIDi 12931214.
    946031.
    KEGGi ecj:Y75_p1455.
    eco:b1479.
    PATRICi 32118252. VBIEscCol129921_1546.

    Organism-specific databases

    EchoBASEi EB0941.
    EcoGenei EG10948. maeA.

    Phylogenomic databases

    eggNOGi COG0281.
    HOGENOMi HOG000042487.
    KOi K00027.
    OMAi IQDNNET.
    OrthoDBi EOG6QVRGM.
    PhylomeDBi P26616.

    Enzyme and pathway databases

    BioCyci EcoCyc:MALIC-NAD-MONOMER.
    ECOL316407:JW5238-MONOMER.
    MetaCyc:MALIC-NAD-MONOMER.
    SABIO-RK P26616.

    Miscellaneous databases

    PROi P26616.

    Gene expression databases

    Genevestigatori P26616.

    Family and domain databases

    Gene3Di 3.40.50.10380. 1 hit.
    3.40.50.720. 1 hit.
    HAMAPi MF_01619. NAD_malic_enz.
    InterProi IPR015884. Malic_enzyme_CS.
    IPR012301. Malic_N_dom.
    IPR012302. Malic_NAD-bd.
    IPR001891. Malic_OxRdtase.
    IPR016040. NAD(P)-bd_dom.
    IPR023667. NAD_malic_enz_proteobac.
    [Graphical view ]
    Pfami PF00390. malic. 1 hit.
    PF03949. Malic_M. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000106. ME. 1 hit.
    PRINTSi PR00072. MALOXRDTASE.
    SMARTi SM00919. Malic_M. 1 hit.
    [Graphical view ]
    PROSITEi PS00331. MALIC_ENZYMES. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    3. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
      Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
      Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    4. "Physical analysis of spontaneous and mutagen-induced mutants of Escherichia coli K-12 expressing DNA exonuclease VIII activity."
      Mahajan S.K., Chu C.C., Willis D.K., Templin A., Clark A.J.
      Genetics 125:261-273(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-431.
      Strain: K12.
    5. "Escherichia coli malic enzymes: two isoforms with substantial differences in kinetic properties, metabolic regulation, and structure."
      Bologna F.P., Andreo C.S., Drincovich M.F.
      J. Bacteriol. 189:5937-5946(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: BIOPHYSICOCHEMICAL PROPERTIES, COFACTOR, ENZYME REGULATION, SUBUNIT.
      Strain: K12.
    6. "Over-expression, purification, and characterization of recombinant NAD-malic enzyme from Escherichia coli K12."
      Wang J., Tan H., Zhao Z.K.
      Protein Expr. Purif. 53:97-103(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: BIOPHYSICOCHEMICAL PROPERTIES, ENZYME REGULATION.
      Strain: K12 / MG1655 / ATCC 47076.

    Entry informationi

    Entry nameiMAO1_ECOLI
    AccessioniPrimary (citable) accession number: P26616
    Secondary accession number(s): P78224
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1992
    Last sequence update: December 6, 2005
    Last modified: October 1, 2014
    This is version 121 of the entry and version 4 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Can also use NADP+ but is more effective with NAD+.

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3