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Protein

Cytoplasmic alpha-amylase

Gene

amyA

Organism
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides containing three or more (1->4)-alpha-linked D-glucose units.By similarity

Cofactori

Ca2+By similarityNote: Binds 1 Ca2+ ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi104CalciumBy similarity1
Metal bindingi198CalciumBy similarity1
Active sitei235NucleophileBy similarity1
Metal bindingi239Calcium; via carbonyl oxygenBy similarity1
Active sitei265Proton donorBy similarity1
Sitei332Transition state stabilizerBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism

Keywords - Ligandi

Calcium, Metal-binding, Sodium

Protein family/group databases

CAZyiGH13. Glycoside Hydrolase Family 13.

Names & Taxonomyi

Protein namesi
Recommended name:
Cytoplasmic alpha-amylase (EC:3.2.1.1By similarity)
Alternative name(s):
1,4-alpha-D-glucan glucanohydrolase
Gene namesi
Name:amyA
Ordered Locus Names:STM1963
OrganismiSalmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Taxonomic identifieri99287 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeSalmonella
Proteomesi
  • UP000001014 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000542881 – 494Cytoplasmic alpha-amylaseAdd BLAST494

Proteomic databases

PaxDbiP26613.

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

STRINGi99287.STM1963.

Structurei

3D structure databases

ProteinModelPortaliP26613.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 13 family.Curated

Phylogenomic databases

eggNOGiENOG4105E5K. Bacteria.
COG0366. LUCA.
HOGENOMiHOG000094847.
KOiK01176.
OMAiFFHWYYP.
PhylomeDBiP26613.

Family and domain databases

Gene3Di2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR006048. A-amylase/branching_C.
IPR013776. A-amylase_thermo.
IPR015902. Glyco_hydro_13.
IPR006047. Glyco_hydro_13_cat_dom.
IPR013780. Glyco_hydro_b.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 2 hits.
PfamiPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
[Graphical view]
PIRSFiPIRSF001021. Alph-amls_thrmst. 1 hit.
SMARTiSM00642. Aamy. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.

Sequencei

Sequence statusi: Complete.

P26613-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKNPTLLQYF HWYYPDGGKL WSELAERADG LNDIGINMVW LPPACKGASG
60 70 80 90 100
GYSVGYDTYD LFDLGEFDQK GTIATKYGDK RQLLTAIDAL KKNNIAVLLD
110 120 130 140 150
VVVNHKMGAD EKERIRVQRV NQDDRTQIDD NIIECEGWTR YTFPARAGQY
160 170 180 190 200
SNFIWDYHCF SGIDHIENPD EDGIFKIVND YTGDGWNDQV DDEMGNFDYL
210 220 230 240 250
MGENIDFRNH AVTEEIKYWA RWVMEQTHCD GFRLDAVKHI PAWFYKEWIE
260 270 280 290 300
HVQAVAPKPL FIVAEYWSHE VDKLQTYIDQ VDGKTMLFDA PLQMKFHEAS
310 320 330 340 350
RQGAEYDMRH IFTGTLVEAD PFHAVTLVAN HDTQPLQALE APVEPWFKPL
360 370 380 390 400
AYALILLREN GVPSVFYPDL YGASYEDSGE NGETCRVDMP VINQLDRLIL
410 420 430 440 450
ARQRFAHGIQ TLFFDHPNCI AFSRSGTEEN PGCVVVLSNG DDGEKTLLLG
460 470 480 490
DNYANKTWRD FLGNRDEYVV TNDQGEATFF CNAGSVSVWV IEDV
Length:494
Mass (Da):56,522
Last modified:January 23, 2002 - v3
Checksum:i5C1E862FEDD5E47C
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti462L → S in AAA27110 (PubMed:1400215).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L01643 Genomic DNA. Translation: AAA27110.1.
AE006468 Genomic DNA. Translation: AAL20875.1.
M85241 Genomic DNA. Translation: AAA27079.1.
L13280 Unassigned DNA. Translation: AAA71970.1.
PIRiB45738.
RefSeqiNP_460916.1. NC_003197.1.
WP_000795487.1. NC_003197.1.

Genome annotation databases

EnsemblBacteriaiAAL20875; AAL20875; STM1963.
GeneIDi1253484.
KEGGistm:STM1963.
PATRICi32382487. VBISalEnt20916_2079.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L01643 Genomic DNA. Translation: AAA27110.1.
AE006468 Genomic DNA. Translation: AAL20875.1.
M85241 Genomic DNA. Translation: AAA27079.1.
L13280 Unassigned DNA. Translation: AAA71970.1.
PIRiB45738.
RefSeqiNP_460916.1. NC_003197.1.
WP_000795487.1. NC_003197.1.

3D structure databases

ProteinModelPortaliP26613.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi99287.STM1963.

Protein family/group databases

CAZyiGH13. Glycoside Hydrolase Family 13.

Proteomic databases

PaxDbiP26613.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAL20875; AAL20875; STM1963.
GeneIDi1253484.
KEGGistm:STM1963.
PATRICi32382487. VBISalEnt20916_2079.

Phylogenomic databases

eggNOGiENOG4105E5K. Bacteria.
COG0366. LUCA.
HOGENOMiHOG000094847.
KOiK01176.
OMAiFFHWYYP.
PhylomeDBiP26613.

Family and domain databases

Gene3Di2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR006048. A-amylase/branching_C.
IPR013776. A-amylase_thermo.
IPR015902. Glyco_hydro_13.
IPR006047. Glyco_hydro_13_cat_dom.
IPR013780. Glyco_hydro_b.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 2 hits.
PfamiPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
[Graphical view]
PIRSFiPIRSF001021. Alph-amls_thrmst. 1 hit.
SMARTiSM00642. Aamy. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiAMY2_SALTY
AccessioniPrimary (citable) accession number: P26613
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: January 23, 2002
Last modified: November 2, 2016
This is version 118 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.