Reviewed, UniProtKB/Swiss-Prot P26613 (AMY2_SALTY)

Names and origin

Protein names

Recommended name:
    Cytoplasmic alpha-amylase
    EC=3.2.1.1
Alternative name(s):
    1,4-alpha-D-glucan glucanohydrolase

Gene names

Name:	amyA
Ordered Locus Names:	STM1963

Organism

Salmonella typhimurium [Complete proteome] [HAMAP]

Taxonomic identifier

90371 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Salmonella

Protein attributes

Sequence length	494 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in oligosaccharides and polysaccharides.
Cofactor	Binds 1 calcium ion per subunit By similarity.
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the glycosyl hydrolase 13 family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism
Cellular component	Cytoplasm
Ligand	Calcium Metal-binding
Molecular function	Glycosidase Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	alpha-amylase activity Inferred from electronic annotation. Source: EC calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 494	494	Cytoplasmic alpha-amylase		PRO_0000054288
Sites
	Active site	235	1	Nucleophile By similarity
	Active site	265	1	Proton donor By similarity
	Active site	332	1	By similarity
	Metal binding	104	1	Calcium By similarity
	Metal binding	239	1	Calcium; via carbonyl oxygen By similarity
Experimental info
	Sequence conflict	462	1	L → S in AAA27110. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P26613-1 [UniParc]. Last modified January 23, 2002. Version 3. Checksum: 5C1E862FEDD5E47C	FASTA	494	56,522
	10 20 30 40 50 60 MKNPTLLQYF HWYYPDGGKL WSELAERADG LNDIGINMVW LPPACKGASG GYSVGYDTYD 70 80 90 100 110 120 LFDLGEFDQK GTIATKYGDK RQLLTAIDAL KKNNIAVLLD VVVNHKMGAD EKERIRVQRV 130 140 150 160 170 180 NQDDRTQIDD NIIECEGWTR YTFPARAGQY SNFIWDYHCF SGIDHIENPD EDGIFKIVND 190 200 210 220 230 240 YTGDGWNDQV DDEMGNFDYL MGENIDFRNH AVTEEIKYWA RWVMEQTHCD GFRLDAVKHI 250 260 270 280 290 300 PAWFYKEWIE HVQAVAPKPL FIVAEYWSHE VDKLQTYIDQ VDGKTMLFDA PLQMKFHEAS 310 320 330 340 350 360 RQGAEYDMRH IFTGTLVEAD PFHAVTLVAN HDTQPLQALE APVEPWFKPL AYALILLREN 370 380 390 400 410 420 GVPSVFYPDL YGASYEDSGE NGETCRVDMP VINQLDRLIL ARQRFAHGIQ TLFFDHPNCI 430 440 450 460 470 480 AFSRSGTEEN PGCVVVLSNG DDGEKTLLLG DNYANKTWRD FLGNRDEYVV TNDQGEATFF 490 CNAGSVSVWV IEDV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Escherichia coli produces a cytoplasmic alpha-amylase, AmyA." Raha M., Kawagishi I., Mueller V., Kihara M., Macnab R.M. J. Bacteriol. 174:6644-6652(1992) [PubMed: 1400215] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: SJW1103.
[2]	"Complete genome sequence of Salmonella enterica serovar Typhimurium LT2." McClelland M., Sanderson K.E., Spieth J., Clifton S.W., Latreille P., Courtney L., Porwollik S., Ali J., Dante M., Du F., Hou S., Layman D., Leonard S., Nguyen C., Scott K., Holmes A., Grewal N., Mulvaney E. , Ryan E., Sun H., Florea L., Miller W., Stoneking T., Nhan M., Waterston R., Wilson R.K. Nature 413:852-856(2001) [PubMed: 11677609] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: LT2 / SGSC1412 / ATCC 700720.
[3]	"Subdivision of flagellar region III of the Escherichia coli and Salmonella typhimurium chromosomes and identification of two additional flagellar genes." Kawagishi I., Mueller V., Williams A.W., Irikura V.M., Macnab R.M. J. Gen. Microbiol. 138:1051-1065(1992) [PubMed: 1527488] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-6. Strain: SJW1103.
[4]	"Organization of the Escherichia coli and Salmonella typhimurium chromosomes between flagellar regions IIIa and IIIb, including a large non-coding region." Raha M., Kihara M., Kawagishi I., Macnab R.M. J. Gen. Microbiol. 139:1401-1407(1993) [PubMed: 8371104] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 476-494.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L01643 Genomic DNA. Translation: AAA27110.1. AE006468 Genomic DNA. Translation: AAL20875.1. M85241 Genomic DNA. Translation: AAA27079.1. L13280 Unassigned DNA. Translation: AAA71970.1.
PIR	B45738.
RefSeq	NP_460916.1.
3D structure databases
HSSP	HSSP built from PDB template 1HVX based on UniProtKB P06279.
ModBase	Search...
Protein family/group databases
CAZy	GH13. Glycoside Hydrolase Family 13.
Genome annotation databases
GeneID	1253484.
GenomeReviews	Gene locus STM1963 in contig AE006468_GR.
KEGG	stm:STM1963.
NMPDR	fig|99287.1.peg.1897.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P26613.
OMA	VWIPPAY.
Enzyme and pathway databases
BioCyc	STYP99287:STM1963-MON.
BRENDA	3.2.1.1. 2.
Family and domain databases
InterPro	IPR013776. A-amylase_thermo. IPR006047. Glyco_hydro_13_cat. IPR006589. Glyco_hydro_13_sub_cat. IPR013781. Glyco_hydro_sg_catalytic. [Graphical view]
Gene3D	G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
Pfam	PF00128. Alpha-amylase. 1 hit. [Graphical view]
PIRSF	PIRSF001021. Alph-amls_thrmst. 1 hit.
SMART	SM00642. Aamy. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AMY2_SALTY

Accession

Primary (citable) accession number: P26613

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1992
Last sequence update:	January 23, 2002
Last modified:	November 3, 2009
This is version 75 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)