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P26613

- AMY2_SALTY

UniProt

P26613 - AMY2_SALTY

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Protein

Cytoplasmic alpha-amylase

Gene

amyA

Organism
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalytic activityi

Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides containing three or more (1->4)-alpha-linked D-glucose units.

Cofactori

Ca2+By similarityNote: Binds 1 Ca(2+) ion per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi104 – 1041CalciumBy similarity
Active sitei235 – 2351NucleophileBy similarity
Metal bindingi239 – 2391Calcium; via carbonyl oxygenBy similarity
Active sitei265 – 2651Proton donorBy similarity
Sitei332 – 3321Transition state stabilizerBy similarity

GO - Molecular functioni

  1. alpha-amylase activity Source: UniProtKB-EC
  2. calcium ion binding Source: InterPro

GO - Biological processi

  1. carbohydrate metabolic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

BioCyciSENT99287:GCTI-1974-MONOMER.

Protein family/group databases

CAZyiGH13. Glycoside Hydrolase Family 13.

Names & Taxonomyi

Protein namesi
Recommended name:
Cytoplasmic alpha-amylase (EC:3.2.1.1)
Alternative name(s):
1,4-alpha-D-glucan glucanohydrolase
Gene namesi
Name:amyA
Ordered Locus Names:STM1963
OrganismiSalmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Taxonomic identifieri99287 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeSalmonella
ProteomesiUP000001014: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 494494Cytoplasmic alpha-amylasePRO_0000054288Add
BLAST

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

STRINGi99287.STM1963.

Structurei

3D structure databases

ProteinModelPortaliP26613.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 13 family.Curated

Phylogenomic databases

eggNOGiCOG0366.
HOGENOMiHOG000094847.
KOiK01176.
OMAiRATKYGD.
OrthoDBiEOG65QWH3.
PhylomeDBiP26613.

Family and domain databases

Gene3Di2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR013776. A-amylase_thermo.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR006589. Glyco_hydro_13_sub_cat_dom.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
[Graphical view]
PIRSFiPIRSF001021. Alph-amls_thrmst. 1 hit.
SMARTiSM00642. Aamy. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.

Sequencei

Sequence statusi: Complete.

P26613-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKNPTLLQYF HWYYPDGGKL WSELAERADG LNDIGINMVW LPPACKGASG
60 70 80 90 100
GYSVGYDTYD LFDLGEFDQK GTIATKYGDK RQLLTAIDAL KKNNIAVLLD
110 120 130 140 150
VVVNHKMGAD EKERIRVQRV NQDDRTQIDD NIIECEGWTR YTFPARAGQY
160 170 180 190 200
SNFIWDYHCF SGIDHIENPD EDGIFKIVND YTGDGWNDQV DDEMGNFDYL
210 220 230 240 250
MGENIDFRNH AVTEEIKYWA RWVMEQTHCD GFRLDAVKHI PAWFYKEWIE
260 270 280 290 300
HVQAVAPKPL FIVAEYWSHE VDKLQTYIDQ VDGKTMLFDA PLQMKFHEAS
310 320 330 340 350
RQGAEYDMRH IFTGTLVEAD PFHAVTLVAN HDTQPLQALE APVEPWFKPL
360 370 380 390 400
AYALILLREN GVPSVFYPDL YGASYEDSGE NGETCRVDMP VINQLDRLIL
410 420 430 440 450
ARQRFAHGIQ TLFFDHPNCI AFSRSGTEEN PGCVVVLSNG DDGEKTLLLG
460 470 480 490
DNYANKTWRD FLGNRDEYVV TNDQGEATFF CNAGSVSVWV IEDV
Length:494
Mass (Da):56,522
Last modified:January 23, 2002 - v3
Checksum:i5C1E862FEDD5E47C
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti462 – 4621L → S in AAA27110. (PubMed:1400215)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L01643 Genomic DNA. Translation: AAA27110.1.
AE006468 Genomic DNA. Translation: AAL20875.1.
M85241 Genomic DNA. Translation: AAA27079.1.
L13280 Unassigned DNA. Translation: AAA71970.1.
PIRiB45738.
RefSeqiNP_460916.1. NC_003197.1.

Genome annotation databases

EnsemblBacteriaiAAL20875; AAL20875; STM1963.
GeneIDi1253484.
KEGGistm:STM1963.
PATRICi32382487. VBISalEnt20916_2079.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L01643 Genomic DNA. Translation: AAA27110.1 .
AE006468 Genomic DNA. Translation: AAL20875.1 .
M85241 Genomic DNA. Translation: AAA27079.1 .
L13280 Unassigned DNA. Translation: AAA71970.1 .
PIRi B45738.
RefSeqi NP_460916.1. NC_003197.1.

3D structure databases

ProteinModelPortali P26613.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 99287.STM1963.

Protein family/group databases

CAZyi GH13. Glycoside Hydrolase Family 13.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAL20875 ; AAL20875 ; STM1963 .
GeneIDi 1253484.
KEGGi stm:STM1963.
PATRICi 32382487. VBISalEnt20916_2079.

Phylogenomic databases

eggNOGi COG0366.
HOGENOMi HOG000094847.
KOi K01176.
OMAi RATKYGD.
OrthoDBi EOG65QWH3.
PhylomeDBi P26613.

Enzyme and pathway databases

BioCyci SENT99287:GCTI-1974-MONOMER.

Family and domain databases

Gene3Di 2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
InterProi IPR013776. A-amylase_thermo.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR006589. Glyco_hydro_13_sub_cat_dom.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
PANTHERi PTHR10357. PTHR10357. 1 hit.
Pfami PF00128. Alpha-amylase. 1 hit.
[Graphical view ]
PIRSFi PIRSF001021. Alph-amls_thrmst. 1 hit.
SMARTi SM00642. Aamy. 1 hit.
[Graphical view ]
SUPFAMi SSF51445. SSF51445. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Escherichia coli produces a cytoplasmic alpha-amylase, AmyA."
    Raha M., Kawagishi I., Mueller V., Kihara M., Macnab R.M.
    J. Bacteriol. 174:6644-6652(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: SJW1103.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: LT2 / SGSC1412 / ATCC 700720.
  3. "Subdivision of flagellar region III of the Escherichia coli and Salmonella typhimurium chromosomes and identification of two additional flagellar genes."
    Kawagishi I., Mueller V., Williams A.W., Irikura V.M., Macnab R.M.
    J. Gen. Microbiol. 138:1051-1065(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-6.
    Strain: SJW1103.
  4. "Organization of the Escherichia coli and Salmonella typhimurium chromosomes between flagellar regions IIIa and IIIb, including a large non-coding region."
    Raha M., Kihara M., Kawagishi I., Macnab R.M.
    J. Gen. Microbiol. 139:1401-1407(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 476-494.

Entry informationi

Entry nameiAMY2_SALTY
AccessioniPrimary (citable) accession number: P26613
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: January 23, 2002
Last modified: November 26, 2014
This is version 110 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3