Reviewed, UniProtKB/Swiss-Prot P26612 (AMY2_ECOLI)

Names and origin

Protein names

Recommended name:
    Cytoplasmic alpha-amylase
    EC=3.2.1.1
Alternative name(s):
    1,4-alpha-D-glucan glucanohydrolase

Gene names

Name:	amyA
Synonyms:	yedC
Ordered Locus Names:	b1927, JW1912

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	495 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in oligosaccharides and polysaccharides.
Cofactor	Binds 1 calcium ion per subunit By similarity.
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the glycosyl hydrolase 13 family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism
Cellular component	Cytoplasm
Ligand	Calcium Metal-binding
Molecular function	Glycosidase Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	alpha-amylase activity Inferred from electronic annotation. Source: EC calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 495	495	Cytoplasmic alpha-amylase		PRO_0000054287
Sites
	Active site	235	1	Nucleophile By similarity
	Active site	265	1	Proton donor By similarity
	Active site	332	1	By similarity
	Metal binding	104	1	Calcium By similarity
	Metal binding	239	1	Calcium; via carbonyl oxygen By similarity
Experimental info
	Sequence conflict	19 – 20	2	KL → SS in AAA23810. Ref.1
	Sequence conflict	109	1	A → V in AAA23810. Ref.1
	Sequence conflict	149	1	Q → E in AAA23810. Ref.1
	Sequence conflict	234	1	L → I in AAA23810. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P26612-1 [UniParc]. Last modified November 1, 1997. Version 3. Checksum: 26AFF6797DDA54D6	FASTA	495	56,639
	10 20 30 40 50 60 MRNPTLLQCF HWYYPEGGKL WPELAERADG FNDIGINMVW LPPAYKGASG GYSVGYDSYD 70 80 90 100 110 120 LFDLGEFDQK GSIPTKYGDK AQLLAAIDAL KRNDIAVLLD VVVNHKMGAD EKEAIRVQRV 130 140 150 160 170 180 NADDRTQIDE EIIECEGWTR YTFPARAGQY SQFIWDFKCF SGIDHIENPD EDGIFKIVND 190 200 210 220 230 240 YTGEGWNDQV DDELGNFDYL MGENIDFRNH AVTEEIKYWA RWVMEQTQCD GFRLDAVKHI 250 260 270 280 290 300 PAWFYKEWIE HVQEVAPKPL FIVAEYWSHE VDKLQTYIDQ VEGKTMLFDA PLQMKFHEAS 310 320 330 340 350 360 RMGRDYDMTQ IFTGTLVEAD PFHAVTLVAN HDTQPLQALE APVEPWFKPL AYALILLREN 370 380 390 400 410 420 GVPSVFYPDL YGAHYEDVGG DGQTYPIDMP IIEQLDELIL ARQRFAHGVQ TLFFDHPNCI 430 440 450 460 470 480 AFSRSGTDEF PGCVVVMSNG DDGEKTIHLG ENYGNKTWRD FLGNRQERVV TDENGEATFF 490 CNGGSVSVWV IEEVI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Escherichia coli produces a cytoplasmic alpha-amylase, AmyA." Raha M., Kawagishi I., Mueller V., Kihara M., Macnab R.M. J. Bacteriol. 174:6644-6652(1992) [PubMed: 1400215] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: JA11.
[2]	"A 460-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 40.1-50.0 min region on the linkage map." Itoh T., Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Kasai H., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T., Mizobuchi K., Mori H., Mori T., Motomura K., Nakade S. , Nakamura Y., Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G., Seki Y., Sivasundaram S., Tagami H., Takeda J., Takemoto K., Wada C., Yamamoto Y., Horiuchi T. DNA Res. 3:379-392(1996) [PubMed: 9097040] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"Subdivision of flagellar region III of the Escherichia coli and Salmonella typhimurium chromosomes and identification of two additional flagellar genes." Kawagishi I., Mueller V., Williams A.W., Irikura V.M., Macnab R.M. J. Gen. Microbiol. 138:1051-1065(1992) [PubMed: 1527488] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-5. Strain: JA11.
[6]	"Organization of the Escherichia coli and Salmonella typhimurium chromosomes between flagellar regions IIIa and IIIb, including a large non-coding region." Raha M., Kihara M., Kawagishi I., Macnab R.M. J. Gen. Microbiol. 139:1401-1407(1993) [PubMed: 8371104] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 475-495. Strain: JA11.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L01642 Genomic DNA. Translation: AAA23810.1. U00096 Genomic DNA. Translation: AAC74994.1. AP009048 Genomic DNA. Translation: BAA15755.1. M85240 Genomic DNA. No translation available. L13279 Genomic DNA. Translation: AAA82575.1.
PIR	A45738. D64956.
RefSeq	AP_002542.1. NP_416437.1.
3D structure databases
HSSP	HSSP built from PDB template 1HVX based on UniProtKB P06279.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:9108N.
Protein family/group databases
CAZy	GH13. Glycoside Hydrolase Family 13.
Genome annotation databases
GeneID	946434.
GenomeReviews	Gene locus JW1912 in contig AP009048_GR. Gene locus b1927 in contig U00096_GR.
KEGG	ecj:JW1912. eco:b1927.
Organism-specific databases
EchoBASE	EB1360.
EcoGene	EG11387. amyA.
CMR	Search...
Phylogenomic databases
HOGENOM	P26612.
OMA	P26612. VWIPPAY.
Enzyme and pathway databases
BioCyc	EcoCyc:ALPHA-AMYL-CYTO-MON. MetaCyc:ALPHA-AMYL-CYTO-MON.
Family and domain databases
InterPro	IPR006048. A-amylase_b_C. IPR013776. A-amylase_thermo. IPR006047. Glyco_hydro_13_cat. IPR006589. Glyco_hydro_13_sub_cat. IPR013781. Glyco_hydro_sg_catalytic. [Graphical view]
Gene3D	G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
Pfam	PF00128. Alpha-amylase. 1 hit. PF02806. Alpha-amylase_C. 1 hit. [Graphical view]
PIRSF	PIRSF001021. Alph-amls_thrmst. 1 hit.
SMART	SM00642. Aamy. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AMY2_ECOLI

Accession

Primary (citable) accession number: P26612
Secondary accession number(s): P78072

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1992
Last sequence update:	November 1, 1997
Last modified:	June 16, 2009
This is version 83 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)