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Protein

Cytoplasmic alpha-amylase

Gene

amyA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides containing three or more (1->4)-alpha-linked D-glucose units.1 Publication

Cofactori

Ca2+By similarityNote: Binds 1 Ca2+ ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi104CalciumBy similarity1
Metal bindingi198CalciumBy similarity1
Active sitei235NucleophileBy similarity1
Metal bindingi239Calcium; via carbonyl oxygenBy similarity1
Active sitei265Proton donorBy similarity1
Sitei332Transition state stabilizerBy similarity1

GO - Molecular functioni

  • alpha-amylase activity Source: EcoCyc
  • calcium ion binding Source: InterPro

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism

Keywords - Ligandi

Calcium, Metal-binding, Sodium

Enzyme and pathway databases

BioCyciEcoCyc:ALPHA-AMYL-CYTO-MONOMER.
ECOL316407:JW1912-MONOMER.
MetaCyc:ALPHA-AMYL-CYTO-MONOMER.

Protein family/group databases

CAZyiGH13. Glycoside Hydrolase Family 13.

Names & Taxonomyi

Protein namesi
Recommended name:
Cytoplasmic alpha-amylase (EC:3.2.1.11 Publication)
Alternative name(s):
1,4-alpha-D-glucan glucanohydrolase
Gene namesi
Name:amyA
Synonyms:yedC
Ordered Locus Names:b1927, JW1912
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11387. amyA.

Subcellular locationi

  • Cytoplasm 1 Publication

GO - Cellular componenti

  • cytoplasm Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000542871 – 495Cytoplasmic alpha-amylaseAdd BLAST495

Proteomic databases

PaxDbiP26612.
PRIDEiP26612.

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

BioGridi4260380. 7 interactors.
DIPiDIP-9108N.
IntActiP26612. 4 interactors.
MINTiMINT-1279253.
STRINGi511145.b1927.

Structurei

3D structure databases

ProteinModelPortaliP26612.
SMRiP26612.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 13 family.Curated

Phylogenomic databases

eggNOGiENOG4105E5K. Bacteria.
COG0366. LUCA.
HOGENOMiHOG000094847.
InParanoidiP26612.
KOiK01176.
OMAiFFHWYYP.
PhylomeDBiP26612.

Family and domain databases

Gene3Di2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR006048. A-amylase/branching_C.
IPR013776. A-amylase_thermo.
IPR015902. Glyco_hydro_13.
IPR006047. Glyco_hydro_13_cat_dom.
IPR013780. Glyco_hydro_b.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 2 hits.
PfamiPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
[Graphical view]
PIRSFiPIRSF001021. Alph-amls_thrmst. 1 hit.
SMARTiSM00642. Aamy. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.

Sequencei

Sequence statusi: Complete.

P26612-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRNPTLLQCF HWYYPEGGKL WPELAERADG FNDIGINMVW LPPAYKGASG
60 70 80 90 100
GYSVGYDSYD LFDLGEFDQK GSIPTKYGDK AQLLAAIDAL KRNDIAVLLD
110 120 130 140 150
VVVNHKMGAD EKEAIRVQRV NADDRTQIDE EIIECEGWTR YTFPARAGQY
160 170 180 190 200
SQFIWDFKCF SGIDHIENPD EDGIFKIVND YTGEGWNDQV DDELGNFDYL
210 220 230 240 250
MGENIDFRNH AVTEEIKYWA RWVMEQTQCD GFRLDAVKHI PAWFYKEWIE
260 270 280 290 300
HVQEVAPKPL FIVAEYWSHE VDKLQTYIDQ VEGKTMLFDA PLQMKFHEAS
310 320 330 340 350
RMGRDYDMTQ IFTGTLVEAD PFHAVTLVAN HDTQPLQALE APVEPWFKPL
360 370 380 390 400
AYALILLREN GVPSVFYPDL YGAHYEDVGG DGQTYPIDMP IIEQLDELIL
410 420 430 440 450
ARQRFAHGVQ TLFFDHPNCI AFSRSGTDEF PGCVVVMSNG DDGEKTIHLG
460 470 480 490
ENYGNKTWRD FLGNRQERVV TDENGEATFF CNGGSVSVWV IEEVI
Length:495
Mass (Da):56,639
Last modified:November 1, 1997 - v3
Checksum:i26AFF6797DDA54D6
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti19 – 20KL → SS in AAA23810 (PubMed:1400215).Curated2
Sequence conflicti109A → V in AAA23810 (PubMed:1400215).Curated1
Sequence conflicti149Q → E in AAA23810 (PubMed:1400215).Curated1
Sequence conflicti234L → I in AAA23810 (PubMed:1400215).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L01642 Genomic DNA. Translation: AAA23810.1.
U00096 Genomic DNA. Translation: AAC74994.1.
AP009048 Genomic DNA. Translation: BAA15755.1.
M85240 Genomic DNA. No translation available.
L13279 Genomic DNA. Translation: AAA82575.1.
PIRiD64956. A45738.
RefSeqiNP_416437.1. NC_000913.3.
WP_001245695.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC74994; AAC74994; b1927.
BAA15755; BAA15755; BAA15755.
GeneIDi946434.
KEGGiecj:JW1912.
eco:b1927.
PATRICi32119189. VBIEscCol129921_2009.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L01642 Genomic DNA. Translation: AAA23810.1.
U00096 Genomic DNA. Translation: AAC74994.1.
AP009048 Genomic DNA. Translation: BAA15755.1.
M85240 Genomic DNA. No translation available.
L13279 Genomic DNA. Translation: AAA82575.1.
PIRiD64956. A45738.
RefSeqiNP_416437.1. NC_000913.3.
WP_001245695.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP26612.
SMRiP26612.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4260380. 7 interactors.
DIPiDIP-9108N.
IntActiP26612. 4 interactors.
MINTiMINT-1279253.
STRINGi511145.b1927.

Protein family/group databases

CAZyiGH13. Glycoside Hydrolase Family 13.

Proteomic databases

PaxDbiP26612.
PRIDEiP26612.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74994; AAC74994; b1927.
BAA15755; BAA15755; BAA15755.
GeneIDi946434.
KEGGiecj:JW1912.
eco:b1927.
PATRICi32119189. VBIEscCol129921_2009.

Organism-specific databases

EchoBASEiEB1360.
EcoGeneiEG11387. amyA.

Phylogenomic databases

eggNOGiENOG4105E5K. Bacteria.
COG0366. LUCA.
HOGENOMiHOG000094847.
InParanoidiP26612.
KOiK01176.
OMAiFFHWYYP.
PhylomeDBiP26612.

Enzyme and pathway databases

BioCyciEcoCyc:ALPHA-AMYL-CYTO-MONOMER.
ECOL316407:JW1912-MONOMER.
MetaCyc:ALPHA-AMYL-CYTO-MONOMER.

Miscellaneous databases

PROiP26612.

Family and domain databases

Gene3Di2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR006048. A-amylase/branching_C.
IPR013776. A-amylase_thermo.
IPR015902. Glyco_hydro_13.
IPR006047. Glyco_hydro_13_cat_dom.
IPR013780. Glyco_hydro_b.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 2 hits.
PfamiPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
[Graphical view]
PIRSFiPIRSF001021. Alph-amls_thrmst. 1 hit.
SMARTiSM00642. Aamy. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiAMY2_ECOLI
AccessioniPrimary (citable) accession number: P26612
Secondary accession number(s): P78072
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: November 1, 1997
Last modified: November 2, 2016
This is version 138 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.