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P26602

- UBIC_ECOLI

UniProt

P26602 - UBIC_ECOLI

Protein

Chorismate pyruvate-lyase

Gene

ubiC

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 129 (01 Oct 2014)
      Sequence version 2 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Removes the pyruvyl group from chorismate, with concomitant aromatization of the ring, to provide 4-hydroxybenzoate (4HB) for the ubiquinone pathway.

    Catalytic activityi

    Chorismate = 4-hydroxybenzoate + pyruvate.

    Enzyme regulationi

    Inhibited by 4-hydroxybenzoate, vanillate, 4-hydroxybenzaldehyde and 3-carboxymethylaminomethyl-4-hydroxybenzoic acid.2 Publications

    Kineticsi

    1. KM=29 µM for chorismate1 Publication

    pH dependencei

    Optimum pH is 7.5.1 Publication

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei35 – 351Substrate; via amide nitrogen
    Binding sitei77 – 771Substrate
    Binding sitei115 – 1151Substrate; via amide nitrogen

    GO - Molecular functioni

    1. chorismate lyase activity Source: EcoCyc

    GO - Biological processi

    1. pyruvate biosynthetic process Source: UniProtKB-HAMAP
    2. ubiquinone biosynthetic process Source: EcoCyc

    Keywords - Molecular functioni

    Lyase

    Keywords - Biological processi

    Ubiquinone biosynthesis

    Enzyme and pathway databases

    BioCyciEcoCyc:CHORPYRLY-MONOMER.
    ECOL316407:JW5713-MONOMER.
    MetaCyc:CHORPYRLY-MONOMER.
    BRENDAi4.1.3.40. 2026.
    UniPathwayiUPA00232.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Chorismate pyruvate-lyase (EC:4.1.3.40)
    Short name:
    CL
    Short name:
    CPL
    Gene namesi
    Name:ubiC
    Ordered Locus Names:b4039, JW5713
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

    Organism-specific databases

    EcoGeneiEG11369. ubiC.

    Subcellular locationi

    GO - Cellular componenti

    1. cytosol Source: EcoCyc

    Keywords - Cellular componenti

    Cytoplasm

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi91 – 911G → A: Increases the inhibition by product by about 40%. No effect on substrate affinity. 1 Publication
    Mutagenesisi156 – 1561E → K: Loss of activity. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 165164Chorismate pyruvate-lyasePRO_0000065711Add
    BLAST

    Proteomic databases

    PaxDbiP26602.
    PRIDEiP26602.

    Expressioni

    Gene expression databases

    GenevestigatoriP26602.

    Interactioni

    Subunit structurei

    Monomer.1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    aceEP0AFG81EBI-559360,EBI-542683

    Protein-protein interaction databases

    DIPiDIP-11066N.
    IntActiP26602. 3 interactions.
    MINTiMINT-1235748.
    STRINGi511145.b4039.

    Structurei

    Secondary structure

    1
    165
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi4 – 118
    Beta strandi13 – 175
    Helixi23 – 308
    Helixi36 – 405
    Turni41 – 433
    Beta strandi46 – 5611
    Helixi58 – 603
    Turni62 – 643
    Helixi65 – 673
    Beta strandi74 – 8310
    Beta strandi86 – 9611
    Helixi97 – 993
    Helixi102 – 1087
    Beta strandi111 – 1133
    Helixi117 – 1193
    Beta strandi121 – 1233
    Beta strandi125 – 13511
    Beta strandi138 – 14710
    Beta strandi150 – 1589

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1FW9X-ray1.40A2-165[»]
    1G1BX-ray1.99A/B2-165[»]
    1G81X-ray1.71A2-165[»]
    1JD3X-ray2.03A2-165[»]
    1TT8X-ray1.00A2-165[»]
    1XLRX-ray1.94A2-165[»]
    2AHCX-ray2.40A/B/C/D2-165[»]
    ProteinModelPortaliP26602.
    SMRiP26602. Positions 2-165.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP26602.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the UbiC family.Curated

    Phylogenomic databases

    eggNOGiCOG3161.
    HOGENOMiHOG000137785.
    KOiK03181.
    OMAiREILLCG.
    OrthoDBiEOG6GBM9Z.

    Family and domain databases

    Gene3Di3.40.1410.10. 1 hit.
    HAMAPiMF_01632. UbiC.
    InterProiIPR007440. Chorismate--pyruvate_lyase.
    IPR028978. Chorismate_lyase_/UTRA_dom.
    [Graphical view]
    PfamiPF04345. Chor_lyase. 1 hit.
    [Graphical view]
    SUPFAMiSSF64288. SSF64288. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P26602-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSHPALTQLR ALRYCKEIPA LDPQLLDWLL LEDSMTKRFE QQGKTVSVTM    50
    IREGFVEQNE IPEELPLLPK ESRYWLREIL LCADGEPWLA GRTVVPVSTL 100
    SGPELALQKL GKTPLGRYLF TSSTLTRDFI EIGRDAGLWG RRSRLRLSGK 150
    PLLLTELFLP ASPLY 165
    Length:165
    Mass (Da):18,777
    Last modified:January 23, 2007 - v2
    Checksum:i6DAACD25BC338F09
    GO

    Sequence cautioni

    The sequence AAC43133.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.
    The sequence CAA40681.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X66619 Genomic DNA. Translation: CAA47181.1.
    M93136 Genomic DNA. Translation: AAA24711.1.
    M93413 Genomic DNA. Translation: AAA24716.1.
    X57434 Genomic DNA. Translation: CAA40681.1. Different initiation.
    M96268 Unassigned DNA. Translation: AAA17027.1.
    DQ087228 Genomic DNA. Translation: AAY88959.1.
    U00006 Genomic DNA. Translation: AAC43133.1. Different initiation.
    U00096 Genomic DNA. Translation: AAC77009.2.
    AP009048 Genomic DNA. Translation: BAE78041.1.
    PIRiS25660.
    RefSeqiNP_418463.4. NC_000913.3.
    YP_492182.1. NC_007779.1.

    Genome annotation databases

    EnsemblBacteriaiAAC77009; AAC77009; b4039.
    BAE78041; BAE78041; BAE78041.
    GeneIDi12933664.
    948545.
    KEGGiecj:Y75_p3926.
    eco:b4039.
    PATRICi32123615. VBIEscCol129921_4156.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X66619 Genomic DNA. Translation: CAA47181.1 .
    M93136 Genomic DNA. Translation: AAA24711.1 .
    M93413 Genomic DNA. Translation: AAA24716.1 .
    X57434 Genomic DNA. Translation: CAA40681.1 . Different initiation.
    M96268 Unassigned DNA. Translation: AAA17027.1 .
    DQ087228 Genomic DNA. Translation: AAY88959.1 .
    U00006 Genomic DNA. Translation: AAC43133.1 . Different initiation.
    U00096 Genomic DNA. Translation: AAC77009.2 .
    AP009048 Genomic DNA. Translation: BAE78041.1 .
    PIRi S25660.
    RefSeqi NP_418463.4. NC_000913.3.
    YP_492182.1. NC_007779.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1FW9 X-ray 1.40 A 2-165 [» ]
    1G1B X-ray 1.99 A/B 2-165 [» ]
    1G81 X-ray 1.71 A 2-165 [» ]
    1JD3 X-ray 2.03 A 2-165 [» ]
    1TT8 X-ray 1.00 A 2-165 [» ]
    1XLR X-ray 1.94 A 2-165 [» ]
    2AHC X-ray 2.40 A/B/C/D 2-165 [» ]
    ProteinModelPortali P26602.
    SMRi P26602. Positions 2-165.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-11066N.
    IntActi P26602. 3 interactions.
    MINTi MINT-1235748.
    STRINGi 511145.b4039.

    Proteomic databases

    PaxDbi P26602.
    PRIDEi P26602.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC77009 ; AAC77009 ; b4039 .
    BAE78041 ; BAE78041 ; BAE78041 .
    GeneIDi 12933664.
    948545.
    KEGGi ecj:Y75_p3926.
    eco:b4039.
    PATRICi 32123615. VBIEscCol129921_4156.

    Organism-specific databases

    EchoBASEi EB1343.
    EcoGenei EG11369. ubiC.

    Phylogenomic databases

    eggNOGi COG3161.
    HOGENOMi HOG000137785.
    KOi K03181.
    OMAi REILLCG.
    OrthoDBi EOG6GBM9Z.

    Enzyme and pathway databases

    UniPathwayi UPA00232 .
    BioCyci EcoCyc:CHORPYRLY-MONOMER.
    ECOL316407:JW5713-MONOMER.
    MetaCyc:CHORPYRLY-MONOMER.
    BRENDAi 4.1.3.40. 2026.

    Miscellaneous databases

    EvolutionaryTracei P26602.
    PROi P26602.

    Gene expression databases

    Genevestigatori P26602.

    Family and domain databases

    Gene3Di 3.40.1410.10. 1 hit.
    HAMAPi MF_01632. UbiC.
    InterProi IPR007440. Chorismate--pyruvate_lyase.
    IPR028978. Chorismate_lyase_/UTRA_dom.
    [Graphical view ]
    Pfami PF04345. Chor_lyase. 1 hit.
    [Graphical view ]
    SUPFAMi SSF64288. SSF64288. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Ubiquinone biosynthesis. Cloning of the genes coding for chorismate pyruvate-lyase and 4-hydroxybenzoate octaprenyl transferase from Escherichia coli."
      Siebert M., Bechthold A., Melzer M., May U., Berger U., Schroeder G., Schroeder J., Severin K., Heide L.
      FEBS Lett. 307:347-350(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: K12 / MC4100 / ATCC 35695 / DSM 6574.
    2. "Cloning and sequencing of Escherichia coli ubiC and purification of chorismate lyase."
      Nichols B.P., Green J.M.
      J. Bacteriol. 174:5309-5316(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-21.
      Strain: K12.
    3. "Location of the ubiA gene on the physical map of Escherichia coli."
      Nishimura K., Nakahigashi K., Inokuchi H.
      J. Bacteriol. 174:5762-5762(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    4. "Mutants of Escherichia coli affected in respiration: the cloning and nucleotide sequence of ubiA, encoding the membrane-bound p-hydroxybenzoate:octaprenyltransferase."
      Wu G., Williams H.D., Gibson F., Poole R.K.
      J. Gen. Microbiol. 139:1795-1805(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: K12.
    5. Zhang D., Shrestha B., Tan T.
      Submitted (JUN-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: BL21.
    6. "Analysis of the Escherichia coli genome. IV. DNA sequence of the region from 89.2 to 92.8 minutes."
      Blattner F.R., Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L.
      Nucleic Acids Res. 21:5408-5417(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    7. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    8. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
      Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
      Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    9. "Formation of 4-hydroxybenzoate in Escherichia coli: characterization of the ubiC gene and its encoded enzyme chorismate pyruvate-lyase."
      Siebert M., Severin K., Heide L.
      Microbiology 140:897-904(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION, MUTAGENESIS OF GLU-156.
    10. Cited for: BIOPHYSICOCHEMICAL PROPERTIES, ENZYME REGULATION.
      Strain: K12 / MG1655 / ATCC 47076.
    11. "Crystallization and 1.1-A diffraction of chorismate lyase from Escherichia coli."
      Stover C., Mayhew M.P., Holden M.J., Howard A., Gallagher D.T.
      J. Struct. Biol. 129:96-99(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.1 ANGSTROMS).
    12. "The crystal structure of chorismate lyase shows a new fold and a tightly retained product."
      Gallagher D.T., Mayhew M., Holden M.J., Howard A., Kim K.-J., Vilker V.L.
      Proteins 44:304-311(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.4 ANGSTROMS) IN COMPLEX WITH PRODUCT; MUTANT SER-15/SER-82 IN COMPLEX WITH PRODUCT; MUTANT SER-15 IN COMPLEX WITH PRODUCT.
    13. Cited for: X-RAY CRYSTALLOGRAPHY (1.0 ANGSTROMS) OF MUTANT SER-15/SER-82 IN COMPLEXES WITH PRODUCT AND SUBSTRATE ANALOG; MUTANT SER-15/SER-82/ALA-91 IN COMPLEXES WITH PRODUCT AND SUBSTRATE ANALOG, MUTAGENESIS OF GLY-91, ENZYME REGULATION.

    Entry informationi

    Entry nameiUBIC_ECOLI
    AccessioniPrimary (citable) accession number: P26602
    Secondary accession number(s): P76783, Q2M6R5, Q4JHR8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1992
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 129 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3