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Protein

Chorismate pyruvate-lyase

Gene

ubiC

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Removes the pyruvyl group from chorismate, with concomitant aromatization of the ring, to provide 4-hydroxybenzoate (4HB) for the ubiquinone pathway.4 Publications

Catalytic activityi

Chorismate = 4-hydroxybenzoate + pyruvate.2 Publications

Enzyme regulationi

Inhibited by 4-hydroxybenzoate, vanillate, 4-hydroxybenzaldehyde and 3-carboxymethylaminomethyl-4-hydroxybenzoic acid.2 Publications

Kineticsi

  1. KM=6.1 µM for chorismate1 Publication
  2. KM=29 µM for chorismate1 Publication

    pH dependencei

    Optimum pH is 7.5.2 Publications

    Pathwayi: ubiquinone biosynthesis

    This protein is involved in the pathway ubiquinone biosynthesis, which is part of Cofactor biosynthesis.
    View all proteins of this organism that are known to be involved in the pathway ubiquinone biosynthesis and in Cofactor biosynthesis.

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Binding sitei35Substrate; via amide nitrogen2 Publications1
    Binding sitei77Substrate2 Publications1
    Binding sitei115Substrate; via amide nitrogen2 Publications1
    Binding sitei156Substrate2 Publications1

    GO - Molecular functioni

    • chorismate lyase activity Source: EcoCyc

    GO - Biological processi

    • ubiquinone biosynthetic process Source: EcoCyc

    Keywordsi

    Molecular functionLyase
    Biological processUbiquinone biosynthesis

    Enzyme and pathway databases

    BioCyciEcoCyc:CHORPYRLY-MONOMER
    MetaCyc:CHORPYRLY-MONOMER
    BRENDAi4.1.3.40 2026
    UniPathwayiUPA00232

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Chorismate pyruvate-lyase (EC:4.1.3.402 Publications)
    Short name:
    CL
    Short name:
    CPL
    Gene namesi
    Name:ubiC
    Ordered Locus Names:b4039, JW5713
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
    Proteomesi
    • UP000000318 Componenti: Chromosome
    • UP000000625 Componenti: Chromosome

    Organism-specific databases

    EcoGeneiEG11369 ubiC

    Subcellular locationi

    GO - Cellular componenti

    • cytosol Source: EcoCyc

    Keywords - Cellular componenti

    Cytoplasm

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Mutagenesisi91G → A: Increases the inhibition by product by about 40%. No effect on substrate affinity. 1 Publication1
    Mutagenesisi156E → K: Loss of activity. 1 Publication1

    Chemistry databases

    DrugBankiDB02130 4-Hydroxy-3-Methoxybenzoate
    DB04242 P-Hydroxybenzoic Acid

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Initiator methionineiRemoved1 Publication
    ChainiPRO_00000657112 – 165Chorismate pyruvate-lyaseAdd BLAST164

    Proteomic databases

    PaxDbiP26602
    PRIDEiP26602

    Interactioni

    Subunit structurei

    Monomer.2 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    aceEP0AFG82EBI-559360,EBI-542683

    Protein-protein interaction databases

    BioGridi4262658, 212 interactors
    DIPiDIP-11066N
    IntActiP26602, 3 interactors
    STRINGi316385.ECDH10B_4228

    Structurei

    Secondary structure

    1165
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details
    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Helixi4 – 11Combined sources8
    Beta strandi13 – 17Combined sources5
    Helixi23 – 30Combined sources8
    Helixi36 – 40Combined sources5
    Turni41 – 43Combined sources3
    Beta strandi46 – 56Combined sources11
    Helixi58 – 60Combined sources3
    Turni62 – 64Combined sources3
    Helixi65 – 67Combined sources3
    Beta strandi74 – 83Combined sources10
    Beta strandi86 – 96Combined sources11
    Helixi97 – 99Combined sources3
    Helixi102 – 108Combined sources7
    Beta strandi111 – 113Combined sources3
    Helixi117 – 119Combined sources3
    Beta strandi121 – 123Combined sources3
    Beta strandi125 – 135Combined sources11
    Beta strandi138 – 147Combined sources10
    Beta strandi150 – 158Combined sources9

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    1FW9X-ray1.40A2-165[»]
    1G1BX-ray1.99A/B2-165[»]
    1G81X-ray1.71A2-165[»]
    1JD3X-ray2.03A2-165[»]
    1TT8X-ray1.00A2-165[»]
    1XLRX-ray1.94A2-165[»]
    2AHCX-ray2.40A/B/C/D2-165[»]
    ProteinModelPortaliP26602
    SMRiP26602
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP26602

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the UbiC family.Curated

    Phylogenomic databases

    eggNOGiENOG4105VEI Bacteria
    COG3161 LUCA
    HOGENOMiHOG000137785
    InParanoidiP26602
    KOiK03181
    OMAiELWGRRS

    Family and domain databases

    Gene3Di3.40.1410.10, 1 hit
    HAMAPiMF_01632 UbiC, 1 hit
    InterProiView protein in InterPro
    IPR007440 Chorismate--pyruvate_lyase
    IPR028978 Chorismate_lyase_/UTRA_dom_sf
    PANTHERiPTHR38683 PTHR38683, 1 hit
    PfamiView protein in Pfam
    PF04345 Chor_lyase, 1 hit
    SUPFAMiSSF64288 SSF64288, 1 hit

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P26602-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MSHPALTQLR ALRYCKEIPA LDPQLLDWLL LEDSMTKRFE QQGKTVSVTM
    60 70 80 90 100
    IREGFVEQNE IPEELPLLPK ESRYWLREIL LCADGEPWLA GRTVVPVSTL
    110 120 130 140 150
    SGPELALQKL GKTPLGRYLF TSSTLTRDFI EIGRDAGLWG RRSRLRLSGK
    160
    PLLLTELFLP ASPLY
    Length:165
    Mass (Da):18,777
    Last modified:January 23, 2007 - v2
    Checksum:i6DAACD25BC338F09
    GO

    Sequence cautioni

    The sequence AAC43133 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
    The sequence CAA40681 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    X66619 Genomic DNA Translation: CAA47181.1
    M93136 Genomic DNA Translation: AAA24711.1
    M93413 Genomic DNA Translation: AAA24716.1
    X57434 Genomic DNA Translation: CAA40681.1 Different initiation.
    M96268 Unassigned DNA Translation: AAA17027.1
    DQ087228 Genomic DNA Translation: AAY88959.1
    U00006 Genomic DNA Translation: AAC43133.1 Different initiation.
    U00096 Genomic DNA Translation: AAC77009.2
    AP009048 Genomic DNA Translation: BAE78041.1
    PIRiS25660
    RefSeqiNP_418463.4, NC_000913.3
    WP_001326644.1, NZ_LN832404.1

    Genome annotation databases

    EnsemblBacteriaiAAC77009; AAC77009; b4039
    BAE78041; BAE78041; BAE78041
    GeneIDi948545
    KEGGiecj:JW5713
    eco:b4039
    PATRICifig|511145.12.peg.4156

    Similar proteinsi

    Entry informationi

    Entry nameiUBIC_ECOLI
    AccessioniPrimary (citable) accession number: P26602
    Secondary accession number(s): P76783, Q2M6R5, Q4JHR8
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1992
    Last sequence update: January 23, 2007
    Last modified: March 28, 2018
    This is version 151 of the entry and version 2 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    4. SIMILARITY comments
      Index of protein domains and families

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