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P26602

- UBIC_ECOLI

UniProt

P26602 - UBIC_ECOLI

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Protein
Chorismate pyruvate-lyase
Gene
ubiC, b4039, JW5713
Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Removes the pyruvyl group from chorismate, with concomitant aromatization of the ring, to provide 4-hydroxybenzoate (4HB) for the ubiquinone pathway.UniRule annotation

Catalytic activityi

Chorismate = 4-hydroxybenzoate + pyruvate.UniRule annotation

Enzyme regulationi

Inhibited by 4-hydroxybenzoate, vanillate, 4-hydroxybenzaldehyde and 3-carboxymethylaminomethyl-4-hydroxybenzoic acid.2 Publications

Kineticsi

  1. KM=29 µM for chorismate1 Publication

pH dependencei

Optimum pH is 7.5.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei35 – 351Substrate; via amide nitrogen
Binding sitei77 – 771Substrate
Binding sitei115 – 1151Substrate; via amide nitrogen

GO - Molecular functioni

  1. chorismate lyase activity Source: EcoCyc

GO - Biological processi

  1. pyruvate biosynthetic process Source: UniProtKB-HAMAP
  2. ubiquinone biosynthetic process Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Ubiquinone biosynthesis

Enzyme and pathway databases

BioCyciEcoCyc:CHORPYRLY-MONOMER.
ECOL316407:JW5713-MONOMER.
MetaCyc:CHORPYRLY-MONOMER.
BRENDAi4.1.3.40. 2026.
UniPathwayiUPA00232.

Names & Taxonomyi

Protein namesi
Recommended name:
Chorismate pyruvate-lyase (EC:4.1.3.40)
Short name:
CL
Short name:
CPL
Gene namesi
Name:ubiC
Ordered Locus Names:b4039, JW5713
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

Organism-specific databases

EcoGeneiEG11369. ubiC.

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytosol Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi91 – 911G → A: Increases the inhibition by product by about 40%. No effect on substrate affinity. 1 Publication
Mutagenesisi156 – 1561E → K: Loss of activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 165164Chorismate pyruvate-lyaseUniRule annotation
PRO_0000065711Add
BLAST

Proteomic databases

PaxDbiP26602.
PRIDEiP26602.

Expressioni

Gene expression databases

GenevestigatoriP26602.

Interactioni

Subunit structurei

Monomer.

Binary interactionsi

WithEntry#Exp.IntActNotes
aceEP0AFG81EBI-559360,EBI-542683

Protein-protein interaction databases

DIPiDIP-11066N.
IntActiP26602. 3 interactions.
MINTiMINT-1235748.
STRINGi511145.b4039.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi4 – 118
Beta strandi13 – 175
Helixi23 – 308
Helixi36 – 405
Turni41 – 433
Beta strandi46 – 5611
Helixi58 – 603
Turni62 – 643
Helixi65 – 673
Beta strandi74 – 8310
Beta strandi86 – 9611
Helixi97 – 993
Helixi102 – 1087
Beta strandi111 – 1133
Helixi117 – 1193
Beta strandi121 – 1233
Beta strandi125 – 13511
Beta strandi138 – 14710
Beta strandi150 – 1589

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1FW9X-ray1.40A2-165[»]
1G1BX-ray1.99A/B2-165[»]
1G81X-ray1.71A2-165[»]
1JD3X-ray2.03A2-165[»]
1TT8X-ray1.00A2-165[»]
1XLRX-ray1.94A2-165[»]
2AHCX-ray2.40A/B/C/D2-165[»]
ProteinModelPortaliP26602.
SMRiP26602. Positions 2-165.

Miscellaneous databases

EvolutionaryTraceiP26602.

Family & Domainsi

Sequence similaritiesi

Belongs to the UbiC family.

Phylogenomic databases

eggNOGiCOG3161.
HOGENOMiHOG000137785.
KOiK03181.
OMAiREILLCG.
OrthoDBiEOG6GBM9Z.

Family and domain databases

Gene3Di3.40.1410.10. 1 hit.
HAMAPiMF_01632. UbiC.
InterProiIPR007440. Chorismate--pyruvate_lyase.
IPR028978. Chorismate_lyase_/UTRA_dom.
[Graphical view]
PfamiPF04345. Chor_lyase. 1 hit.
[Graphical view]
SUPFAMiSSF64288. SSF64288. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P26602-1 [UniParc]FASTAAdd to Basket

« Hide

MSHPALTQLR ALRYCKEIPA LDPQLLDWLL LEDSMTKRFE QQGKTVSVTM    50
IREGFVEQNE IPEELPLLPK ESRYWLREIL LCADGEPWLA GRTVVPVSTL 100
SGPELALQKL GKTPLGRYLF TSSTLTRDFI EIGRDAGLWG RRSRLRLSGK 150
PLLLTELFLP ASPLY 165
Length:165
Mass (Da):18,777
Last modified:January 23, 2007 - v2
Checksum:i6DAACD25BC338F09
GO

Sequence cautioni

The sequence AAC43133.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.
The sequence CAA40681.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X66619 Genomic DNA. Translation: CAA47181.1.
M93136 Genomic DNA. Translation: AAA24711.1.
M93413 Genomic DNA. Translation: AAA24716.1.
X57434 Genomic DNA. Translation: CAA40681.1. Different initiation.
M96268 Unassigned DNA. Translation: AAA17027.1.
DQ087228 Genomic DNA. Translation: AAY88959.1.
U00006 Genomic DNA. Translation: AAC43133.1. Different initiation.
U00096 Genomic DNA. Translation: AAC77009.2.
AP009048 Genomic DNA. Translation: BAE78041.1.
PIRiS25660.
RefSeqiNP_418463.4. NC_000913.3.
YP_492182.1. NC_007779.1.

Genome annotation databases

EnsemblBacteriaiAAC77009; AAC77009; b4039.
BAE78041; BAE78041; BAE78041.
GeneIDi12933664.
948545.
KEGGiecj:Y75_p3926.
eco:b4039.
PATRICi32123615. VBIEscCol129921_4156.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X66619 Genomic DNA. Translation: CAA47181.1 .
M93136 Genomic DNA. Translation: AAA24711.1 .
M93413 Genomic DNA. Translation: AAA24716.1 .
X57434 Genomic DNA. Translation: CAA40681.1 . Different initiation.
M96268 Unassigned DNA. Translation: AAA17027.1 .
DQ087228 Genomic DNA. Translation: AAY88959.1 .
U00006 Genomic DNA. Translation: AAC43133.1 . Different initiation.
U00096 Genomic DNA. Translation: AAC77009.2 .
AP009048 Genomic DNA. Translation: BAE78041.1 .
PIRi S25660.
RefSeqi NP_418463.4. NC_000913.3.
YP_492182.1. NC_007779.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1FW9 X-ray 1.40 A 2-165 [» ]
1G1B X-ray 1.99 A/B 2-165 [» ]
1G81 X-ray 1.71 A 2-165 [» ]
1JD3 X-ray 2.03 A 2-165 [» ]
1TT8 X-ray 1.00 A 2-165 [» ]
1XLR X-ray 1.94 A 2-165 [» ]
2AHC X-ray 2.40 A/B/C/D 2-165 [» ]
ProteinModelPortali P26602.
SMRi P26602. Positions 2-165.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-11066N.
IntActi P26602. 3 interactions.
MINTi MINT-1235748.
STRINGi 511145.b4039.

Proteomic databases

PaxDbi P26602.
PRIDEi P26602.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC77009 ; AAC77009 ; b4039 .
BAE78041 ; BAE78041 ; BAE78041 .
GeneIDi 12933664.
948545.
KEGGi ecj:Y75_p3926.
eco:b4039.
PATRICi 32123615. VBIEscCol129921_4156.

Organism-specific databases

EchoBASEi EB1343.
EcoGenei EG11369. ubiC.

Phylogenomic databases

eggNOGi COG3161.
HOGENOMi HOG000137785.
KOi K03181.
OMAi REILLCG.
OrthoDBi EOG6GBM9Z.

Enzyme and pathway databases

UniPathwayi UPA00232 .
BioCyci EcoCyc:CHORPYRLY-MONOMER.
ECOL316407:JW5713-MONOMER.
MetaCyc:CHORPYRLY-MONOMER.
BRENDAi 4.1.3.40. 2026.

Miscellaneous databases

EvolutionaryTracei P26602.
PROi P26602.

Gene expression databases

Genevestigatori P26602.

Family and domain databases

Gene3Di 3.40.1410.10. 1 hit.
HAMAPi MF_01632. UbiC.
InterProi IPR007440. Chorismate--pyruvate_lyase.
IPR028978. Chorismate_lyase_/UTRA_dom.
[Graphical view ]
Pfami PF04345. Chor_lyase. 1 hit.
[Graphical view ]
SUPFAMi SSF64288. SSF64288. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Ubiquinone biosynthesis. Cloning of the genes coding for chorismate pyruvate-lyase and 4-hydroxybenzoate octaprenyl transferase from Escherichia coli."
    Siebert M., Bechthold A., Melzer M., May U., Berger U., Schroeder G., Schroeder J., Severin K., Heide L.
    FEBS Lett. 307:347-350(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12 / MC4100 / ATCC 35695 / DSM 6574.
  2. "Cloning and sequencing of Escherichia coli ubiC and purification of chorismate lyase."
    Nichols B.P., Green J.M.
    J. Bacteriol. 174:5309-5316(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-21.
    Strain: K12.
  3. "Location of the ubiA gene on the physical map of Escherichia coli."
    Nishimura K., Nakahigashi K., Inokuchi H.
    J. Bacteriol. 174:5762-5762(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  4. "Mutants of Escherichia coli affected in respiration: the cloning and nucleotide sequence of ubiA, encoding the membrane-bound p-hydroxybenzoate:octaprenyltransferase."
    Wu G., Williams H.D., Gibson F., Poole R.K.
    J. Gen. Microbiol. 139:1795-1805(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12.
  5. Zhang D., Shrestha B., Tan T.
    Submitted (JUN-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: BL21.
  6. "Analysis of the Escherichia coli genome. IV. DNA sequence of the region from 89.2 to 92.8 minutes."
    Blattner F.R., Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L.
    Nucleic Acids Res. 21:5408-5417(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  7. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  8. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  9. "Formation of 4-hydroxybenzoate in Escherichia coli: characterization of the ubiC gene and its encoded enzyme chorismate pyruvate-lyase."
    Siebert M., Severin K., Heide L.
    Microbiology 140:897-904(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION, MUTAGENESIS OF GLU-156.
  10. Cited for: BIOPHYSICOCHEMICAL PROPERTIES, ENZYME REGULATION.
    Strain: K12 / MG1655 / ATCC 47076.
  11. "Crystallization and 1.1-A diffraction of chorismate lyase from Escherichia coli."
    Stover C., Mayhew M.P., Holden M.J., Howard A., Gallagher D.T.
    J. Struct. Biol. 129:96-99(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.1 ANGSTROMS).
  12. "The crystal structure of chorismate lyase shows a new fold and a tightly retained product."
    Gallagher D.T., Mayhew M., Holden M.J., Howard A., Kim K.-J., Vilker V.L.
    Proteins 44:304-311(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.4 ANGSTROMS) IN COMPLEX WITH PRODUCT; MUTANT SER-15/SER-82 IN COMPLEX WITH PRODUCT; MUTANT SER-15 IN COMPLEX WITH PRODUCT.
  13. Cited for: X-RAY CRYSTALLOGRAPHY (1.0 ANGSTROMS) OF MUTANT SER-15/SER-82 IN COMPLEXES WITH PRODUCT AND SUBSTRATE ANALOG; MUTANT SER-15/SER-82/ALA-91 IN COMPLEXES WITH PRODUCT AND SUBSTRATE ANALOG, MUTAGENESIS OF GLY-91, ENZYME REGULATION.

Entry informationi

Entry nameiUBIC_ECOLI
AccessioniPrimary (citable) accession number: P26602
Secondary accession number(s): P76783, Q2M6R5, Q4JHR8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: January 23, 2007
Last modified: June 11, 2014
This is version 128 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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