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Protein

Chorismate pyruvate-lyase

Gene

ubiC

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Removes the pyruvyl group from chorismate, with concomitant aromatization of the ring, to provide 4-hydroxybenzoate (4HB) for the ubiquinone pathway.4 Publications

Catalytic activityi

Chorismate = 4-hydroxybenzoate + pyruvate.2 Publications

Enzyme regulationi

Inhibited by 4-hydroxybenzoate, vanillate, 4-hydroxybenzaldehyde and 3-carboxymethylaminomethyl-4-hydroxybenzoic acid.2 Publications

Kineticsi

  1. KM=6.1 µM for chorismate1 Publication
  2. KM=29 µM for chorismate1 Publication

    pH dependencei

    Optimum pH is 7.5.2 Publications

    Pathwayi: ubiquinone biosynthesis

    This protein is involved in the pathway ubiquinone biosynthesis, which is part of Cofactor biosynthesis.
    View all proteins of this organism that are known to be involved in the pathway ubiquinone biosynthesis and in Cofactor biosynthesis.

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Binding sitei35Substrate; via amide nitrogen2 Publications1
    Binding sitei77Substrate2 Publications1
    Binding sitei115Substrate; via amide nitrogen2 Publications1
    Binding sitei156Substrate2 Publications1

    GO - Molecular functioni

    • chorismate lyase activity Source: EcoCyc

    GO - Biological processi

    • pyruvate biosynthetic process Source: UniProtKB-HAMAP
    • ubiquinone biosynthetic process Source: EcoCyc
    Complete GO annotation...

    Keywords - Molecular functioni

    Lyase

    Keywords - Biological processi

    Ubiquinone biosynthesis

    Enzyme and pathway databases

    BioCyciEcoCyc:CHORPYRLY-MONOMER.
    ECOL316407:JW5713-MONOMER.
    MetaCyc:CHORPYRLY-MONOMER.
    BRENDAi4.1.3.40. 2026.
    UniPathwayiUPA00232.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Chorismate pyruvate-lyase (EC:4.1.3.402 Publications)
    Short name:
    CL
    Short name:
    CPL
    Gene namesi
    Name:ubiC
    Ordered Locus Names:b4039, JW5713
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
    Proteomesi
    • UP000000318 Componenti: Chromosome
    • UP000000625 Componenti: Chromosome

    Organism-specific databases

    EcoGeneiEG11369. ubiC.

    Subcellular locationi

    GO - Cellular componenti

    • cytosol Source: EcoCyc
    Complete GO annotation...

    Keywords - Cellular componenti

    Cytoplasm

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Mutagenesisi91G → A: Increases the inhibition by product by about 40%. No effect on substrate affinity. 1 Publication1
    Mutagenesisi156E → K: Loss of activity. 1 Publication1

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Initiator methionineiRemoved1 Publication
    ChainiPRO_00000657112 – 165Chorismate pyruvate-lyaseAdd BLAST164

    Proteomic databases

    PaxDbiP26602.
    PRIDEiP26602.

    Interactioni

    Subunit structurei

    Monomer.2 Publications

    Protein-protein interaction databases

    BioGridi4262658. 210 interactors.
    DIPiDIP-11066N.
    IntActiP26602. 3 interactors.
    MINTiMINT-1235748.
    STRINGi511145.b4039.

    Structurei

    Secondary structure

    1165
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details
    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Helixi4 – 11Combined sources8
    Beta strandi13 – 17Combined sources5
    Helixi23 – 30Combined sources8
    Helixi36 – 40Combined sources5
    Turni41 – 43Combined sources3
    Beta strandi46 – 56Combined sources11
    Helixi58 – 60Combined sources3
    Turni62 – 64Combined sources3
    Helixi65 – 67Combined sources3
    Beta strandi74 – 83Combined sources10
    Beta strandi86 – 96Combined sources11
    Helixi97 – 99Combined sources3
    Helixi102 – 108Combined sources7
    Beta strandi111 – 113Combined sources3
    Helixi117 – 119Combined sources3
    Beta strandi121 – 123Combined sources3
    Beta strandi125 – 135Combined sources11
    Beta strandi138 – 147Combined sources10
    Beta strandi150 – 158Combined sources9

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    1FW9X-ray1.40A2-165[»]
    1G1BX-ray1.99A/B2-165[»]
    1G81X-ray1.71A2-165[»]
    1JD3X-ray2.03A2-165[»]
    1TT8X-ray1.00A2-165[»]
    1XLRX-ray1.94A2-165[»]
    2AHCX-ray2.40A/B/C/D2-165[»]
    ProteinModelPortaliP26602.
    SMRiP26602.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP26602.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the UbiC family.Curated

    Phylogenomic databases

    eggNOGiENOG4105VEI. Bacteria.
    COG3161. LUCA.
    HOGENOMiHOG000137785.
    InParanoidiP26602.
    KOiK03181.
    OMAiELWGRRS.

    Family and domain databases

    Gene3Di3.40.1410.10. 1 hit.
    HAMAPiMF_01632. UbiC. 1 hit.
    InterProiIPR007440. Chorismate--pyruvate_lyase.
    IPR028978. Chorismate_lyase_/UTRA_dom.
    [Graphical view]
    PfamiPF04345. Chor_lyase. 1 hit.
    [Graphical view]
    SUPFAMiSSF64288. SSF64288. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P26602-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MSHPALTQLR ALRYCKEIPA LDPQLLDWLL LEDSMTKRFE QQGKTVSVTM
    60 70 80 90 100
    IREGFVEQNE IPEELPLLPK ESRYWLREIL LCADGEPWLA GRTVVPVSTL
    110 120 130 140 150
    SGPELALQKL GKTPLGRYLF TSSTLTRDFI EIGRDAGLWG RRSRLRLSGK
    160
    PLLLTELFLP ASPLY
    Length:165
    Mass (Da):18,777
    Last modified:January 23, 2007 - v2
    Checksum:i6DAACD25BC338F09
    GO

    Sequence cautioni

    The sequence AAC43133 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
    The sequence CAA40681 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    X66619 Genomic DNA. Translation: CAA47181.1.
    M93136 Genomic DNA. Translation: AAA24711.1.
    M93413 Genomic DNA. Translation: AAA24716.1.
    X57434 Genomic DNA. Translation: CAA40681.1. Different initiation.
    M96268 Unassigned DNA. Translation: AAA17027.1.
    DQ087228 Genomic DNA. Translation: AAY88959.1.
    U00006 Genomic DNA. Translation: AAC43133.1. Different initiation.
    U00096 Genomic DNA. Translation: AAC77009.2.
    AP009048 Genomic DNA. Translation: BAE78041.1.
    PIRiS25660.
    RefSeqiNP_418463.4. NC_000913.3.
    WP_001326644.1. NZ_LN832404.1.

    Genome annotation databases

    EnsemblBacteriaiAAC77009; AAC77009; b4039.
    BAE78041; BAE78041; BAE78041.
    GeneIDi948545.
    KEGGiecj:JW5713.
    eco:b4039.
    PATRICi32123615. VBIEscCol129921_4156.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    X66619 Genomic DNA. Translation: CAA47181.1.
    M93136 Genomic DNA. Translation: AAA24711.1.
    M93413 Genomic DNA. Translation: AAA24716.1.
    X57434 Genomic DNA. Translation: CAA40681.1. Different initiation.
    M96268 Unassigned DNA. Translation: AAA17027.1.
    DQ087228 Genomic DNA. Translation: AAY88959.1.
    U00006 Genomic DNA. Translation: AAC43133.1. Different initiation.
    U00096 Genomic DNA. Translation: AAC77009.2.
    AP009048 Genomic DNA. Translation: BAE78041.1.
    PIRiS25660.
    RefSeqiNP_418463.4. NC_000913.3.
    WP_001326644.1. NZ_LN832404.1.

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    1FW9X-ray1.40A2-165[»]
    1G1BX-ray1.99A/B2-165[»]
    1G81X-ray1.71A2-165[»]
    1JD3X-ray2.03A2-165[»]
    1TT8X-ray1.00A2-165[»]
    1XLRX-ray1.94A2-165[»]
    2AHCX-ray2.40A/B/C/D2-165[»]
    ProteinModelPortaliP26602.
    SMRiP26602.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    BioGridi4262658. 210 interactors.
    DIPiDIP-11066N.
    IntActiP26602. 3 interactors.
    MINTiMINT-1235748.
    STRINGi511145.b4039.

    Proteomic databases

    PaxDbiP26602.
    PRIDEiP26602.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblBacteriaiAAC77009; AAC77009; b4039.
    BAE78041; BAE78041; BAE78041.
    GeneIDi948545.
    KEGGiecj:JW5713.
    eco:b4039.
    PATRICi32123615. VBIEscCol129921_4156.

    Organism-specific databases

    EchoBASEiEB1343.
    EcoGeneiEG11369. ubiC.

    Phylogenomic databases

    eggNOGiENOG4105VEI. Bacteria.
    COG3161. LUCA.
    HOGENOMiHOG000137785.
    InParanoidiP26602.
    KOiK03181.
    OMAiELWGRRS.

    Enzyme and pathway databases

    UniPathwayiUPA00232.
    BioCyciEcoCyc:CHORPYRLY-MONOMER.
    ECOL316407:JW5713-MONOMER.
    MetaCyc:CHORPYRLY-MONOMER.
    BRENDAi4.1.3.40. 2026.

    Miscellaneous databases

    EvolutionaryTraceiP26602.
    PROiP26602.

    Family and domain databases

    Gene3Di3.40.1410.10. 1 hit.
    HAMAPiMF_01632. UbiC. 1 hit.
    InterProiIPR007440. Chorismate--pyruvate_lyase.
    IPR028978. Chorismate_lyase_/UTRA_dom.
    [Graphical view]
    PfamiPF04345. Chor_lyase. 1 hit.
    [Graphical view]
    SUPFAMiSSF64288. SSF64288. 1 hit.
    ProtoNetiSearch...

    Entry informationi

    Entry nameiUBIC_ECOLI
    AccessioniPrimary (citable) accession number: P26602
    Secondary accession number(s): P76783, Q2M6R5, Q4JHR8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1992
    Last sequence update: January 23, 2007
    Last modified: November 2, 2016
    This is version 142 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    4. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.