Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Polypyrimidine tract-binding protein 1

Gene

PTBP1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plays a role in pre-mRNA splicing and in the regulation of alternative splicing events. Activates exon skipping of its own pre-mRNA during muscle cell differentiation. Binds to the polypyrimidine tract of introns. May promote RNA looping when bound to two separate polypyrimidine tracts in the same pre-mRNA. May promote the binding of U2 snRNP to pre-mRNA. Cooperates with RAVER1 to modulate switching between mutually exclusive exons during maturation of the TPM1 pre-mRNA. Represses the splicing of MAPT/Tau exon 10 (PubMed:15009664). In case of infection by picornaviruses, binds to the viral internal ribosome entry site (IRES) and stimulates the IRES-mediated translation (PubMed:21518806).6 Publications

GO - Molecular functioni

  • nucleotide binding Source: InterPro
  • poly(A) RNA binding Source: UniProtKB
  • poly-pyrimidine tract binding Source: ProtInc
  • pre-mRNA binding Source: UniProtKB
  • RNA binding Source: ProtInc

GO - Biological processi

  • alternative mRNA splicing, via spliceosome Source: Ensembl
  • fibroblast growth factor receptor signaling pathway Source: Reactome
  • gene expression Source: Reactome
  • IRES-dependent viral translational initiation Source: UniProtKB
  • mRNA processing Source: ProtInc
  • mRNA splicing, via spliceosome Source: Reactome
  • negative regulation of mRNA splicing, via spliceosome Source: UniProtKB
  • negative regulation of muscle cell differentiation Source: UniProtKB
  • negative regulation of RNA splicing Source: UniProtKB
  • regulation of alternative mRNA splicing, via spliceosome Source: UniProtKB
  • RNA splicing Source: ProtInc
Complete GO annotation...

Keywords - Molecular functioni

Activator, Repressor

Keywords - Biological processi

mRNA processing, mRNA splicing

Keywords - Ligandi

RNA-binding

Enzyme and pathway databases

BioCyciZFISH:ENSG00000011304-MONOMER.
ReactomeiR-HSA-6803529. FGFR2 alternative splicing.
R-HSA-72163. mRNA Splicing - Major Pathway.
R-HSA-72203. Processing of Capped Intron-Containing Pre-mRNA.
SIGNORiP26599.

Names & Taxonomyi

Protein namesi
Recommended name:
Polypyrimidine tract-binding protein 1
Short name:
PTB
Alternative name(s):
57 kDa RNA-binding protein PPTB-1
Heterogeneous nuclear ribonucleoprotein I
Short name:
hnRNP I
Gene namesi
Name:PTBP1
Synonyms:PTB
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 19

Organism-specific databases

HGNCiHGNC:9583. PTBP1.

Subcellular locationi

GO - Cellular componenti

  • extracellular exosome Source: UniProtKB
  • membrane Source: UniProtKB
  • nucleolus Source: ProtInc
  • nucleoplasm Source: Reactome
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi5725.
OpenTargetsiENSG00000011304.
PharmGKBiPA33934.

Chemistry databases

ChEMBLiCHEMBL1293230.

Polymorphism and mutation databases

BioMutaiPTBP1.
DMDMi131528.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000817371 – 531Polypyrimidine tract-binding protein 1Add BLAST531

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineCombined sources1 Publication1
Modified residuei16PhosphoserineCombined sources1
Modified residuei127PhosphotyrosineCombined sources1
Modified residuei138PhosphothreonineCombined sources1
Modified residuei141PhosphoserineCombined sources1
Modified residuei433PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiP26599.
MaxQBiP26599.
PaxDbiP26599.
PeptideAtlasiP26599.
PRIDEiP26599.
TopDownProteomicsiP26599-1. [P26599-1]
P26599-2. [P26599-2]

2D gel databases

SWISS-2DPAGEP26599.

PTM databases

iPTMnetiP26599.
PhosphoSitePlusiP26599.
SwissPalmiP26599.

Miscellaneous databases

PMAP-CutDBP26599.

Expressioni

Gene expression databases

BgeeiENSG00000011304.
CleanExiHS_PTBP1.
ExpressionAtlasiP26599. baseline and differential.
GenevisibleiP26599. HS.

Organism-specific databases

HPAiCAB013507.

Interactioni

Subunit structurei

Monomer. Part of a ternary complex containing KHSRP, PTBP1, PTBP2 and HNRPH1. Interacts with RAVER1 and SFPQ.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
P299915EBI-350540,EBI-8826689From a different organism.
hnrnpabQ7ZYE94EBI-350540,EBI-1086317From a different organism.
PCBP1Q153652EBI-350540,EBI-946095
QKIQ96PU83EBI-350540,EBI-945792
SFPQP232462EBI-350540,EBI-355453
SNRPAP090125EBI-350540,EBI-607085

Protein-protein interaction databases

BioGridi111697. 127 interactors.
IntActiP26599. 48 interactors.
MINTiMINT-1348690.
STRINGi9606.ENSP00000349428.

Structurei

Secondary structure

1531
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi60 – 63Combined sources4
Helixi72 – 82Combined sources11
Beta strandi85 – 91Combined sources7
Turni92 – 95Combined sources4
Beta strandi96 – 103Combined sources8
Helixi104 – 114Combined sources11
Beta strandi127 – 130Combined sources4
Beta strandi132 – 135Combined sources4
Turni142 – 145Combined sources4
Beta strandi182 – 190Combined sources9
Helixi197 – 204Combined sources8
Helixi205 – 207Combined sources3
Beta strandi210 – 218Combined sources9
Beta strandi221 – 229Combined sources9
Helixi231 – 241Combined sources11
Beta strandi245 – 247Combined sources3
Beta strandi250 – 257Combined sources8
Beta strandi259 – 262Combined sources4
Beta strandi269 – 274Combined sources6
Turni289 – 291Combined sources3
Turni294 – 296Combined sources3
Helixi329 – 332Combined sources4
Beta strandi339 – 342Combined sources4
Beta strandi346 – 348Combined sources3
Helixi351 – 359Combined sources9
Beta strandi365 – 368Combined sources4
Turni371 – 373Combined sources3
Beta strandi378 – 380Combined sources3
Turni382 – 384Combined sources3
Helixi385 – 394Combined sources10
Beta strandi400 – 403Combined sources4
Beta strandi405 – 408Combined sources4
Beta strandi417 – 425Combined sources9
Beta strandi427 – 431Combined sources5
Beta strandi437 – 440Combined sources4
Helixi444 – 446Combined sources3
Beta strandi455 – 458Combined sources4
Helixi467 – 476Combined sources10
Beta strandi483 – 488Combined sources6
Beta strandi494 – 497Combined sources4
Helixi501 – 511Combined sources11
Beta strandi515 – 519Combined sources5
Beta strandi524 – 527Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1QM9NMR-A335-531[»]
1SJQNMR-A55-147[»]
1SJRNMR-A147-301[»]
2AD9NMR-A49-146[»]
2ADBNMR-A172-298[»]
2ADCNMR-A324-531[»]
2EVZNMR-A324-531[»]
2N3ONMR-A41-163[»]
3ZZYX-ray1.40A/B156-285[»]
3ZZZX-ray1.55A/B156-285[»]
ProteinModelPortaliP26599.
SMRiP26599.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP26599.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini59 – 143RRM 1PROSITE-ProRule annotationAdd BLAST85
Domaini184 – 260RRM 2PROSITE-ProRule annotationAdd BLAST77
Domaini337 – 411RRM 3PROSITE-ProRule annotationAdd BLAST75
Domaini454 – 529RRM 4PROSITE-ProRule annotationAdd BLAST76

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi316 – 323Poly-Ala8

Sequence similaritiesi

Contains 4 RRM (RNA recognition motif) domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG1190. Eukaryota.
ENOG410XPMZ. LUCA.
GeneTreeiENSGT00550000074508.
HOGENOMiHOG000231699.
HOVERGENiHBG069548.
InParanoidiP26599.
KOiK13218.
OMAiMSAQHAK.
OrthoDBiEOG091G0Z5R.
PhylomeDBiP26599.
TreeFamiTF319824.

Family and domain databases

Gene3Di3.30.70.330. 4 hits.
InterProiIPR006536. HnRNP-L/PTB.
IPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PfamiPF00076. RRM_1. 2 hits.
[Graphical view]
SMARTiSM00360. RRM. 4 hits.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 4 hits.
TIGRFAMsiTIGR01649. hnRNP-L_PTB. 1 hit.
PROSITEiPS50102. RRM. 4 hits.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Note: Additional isoforms seem to exist.
Isoform 1 (identifier: P26599-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDGIVPDIAV GTKRGSDELF STCVTNGPFI MSSNSASAAN GNDSKKFKGD
60 70 80 90 100
SRSAGVPSRV IHIRKLPIDV TEGEVISLGL PFGKVTNLLM LKGKNQAFIE
110 120 130 140 150
MNTEEAANTM VNYYTSVTPV LRGQPIYIQF SNHKELKTDS SPNQARAQAA
160 170 180 190 200
LQAVNSVQSG NLALAASAAA VDAGMAMAGQ SPVLRIIVEN LFYPVTLDVL
210 220 230 240 250
HQIFSKFGTV LKIITFTKNN QFQALLQYAD PVSAQHAKLS LDGQNIYNAC
260 270 280 290 300
CTLRIDFSKL TSLNVKYNND KSRDYTRPDL PSGDSQPSLD QTMAAAFGLS
310 320 330 340 350
VPNVHGALAP LAIPSAAAAA AAAGRIAIPG LAGAGNSVLL VSNLNPERVT
360 370 380 390 400
PQSLFILFGV YGDVQRVKIL FNKKENALVQ MADGNQAQLA MSHLNGHKLH
410 420 430 440 450
GKPIRITLSK HQNVQLPREG QEDQGLTKDY GNSPLHRFKK PGSKNFQNIF
460 470 480 490 500
PPSATLHLSN IPPSVSEEDL KVLFSSNGGV VKGFKFFQKD RKMALIQMGS
510 520 530
VEEAVQALID LHNHDLGENH HLRVSFSKST I
Length:531
Mass (Da):57,221
Last modified:August 1, 1992 - v1
Checksum:iBE12D5EA21537BED
GO
Isoform 2 (identifier: P26599-2) [UniParc]FASTAAdd to basket
Also known as: PTB2

The sequence of this isoform differs from the canonical sequence as follows:
     297-297: F → FASPYAGAGFPPTFAIPQAA

Show »
Length:550
Mass (Da):59,037
Checksum:i70CB96D0530CA091
GO
Isoform 3 (identifier: P26599-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     297-297: F → FGAPGIISASPYAGAGFPPTFAIPQAA

Show »
Length:557
Mass (Da):59,633
Checksum:iDAFE52BAC0E4BE5C
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_005802297F → FASPYAGAGFPPTFAIPQAA in isoform 2. Curated1
Alternative sequenceiVSP_043649297F → FGAPGIISASPYAGAGFPPT FAIPQAA in isoform 3. 2 Publications1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X62006 mRNA. Translation: CAA43973.1.
X65371 mRNA. Translation: CAA46443.1.
X65372 mRNA. Translation: CAA46444.1.
X60648 mRNA. Translation: CAA43056.1.
X66975 mRNA. Translation: CAA47386.1.
BT006819 mRNA. Translation: AAP35465.1.
AC006273 Genomic DNA. Translation: AAC99798.1.
CH471242 Genomic DNA. Translation: EAW61154.1.
BC002397 mRNA. Translation: AAH02397.1.
BC004383 mRNA. Translation: AAH04383.1.
BC013694 mRNA. Translation: AAH13694.1.
CCDSiCCDS32859.1. [P26599-1]
CCDS42456.1. [P26599-3]
CCDS45892.1. [P26599-2]
PIRiS23016.
RefSeqiNP_002810.1. NM_002819.4. [P26599-3]
NP_114367.1. NM_031990.3. [P26599-2]
NP_114368.1. NM_031991.3. [P26599-1]
UniGeneiHs.172550.

Genome annotation databases

EnsembliENST00000349038; ENSP00000014112; ENSG00000011304. [P26599-1]
ENST00000356948; ENSP00000349428; ENSG00000011304. [P26599-3]
ENST00000394601; ENSP00000408096; ENSG00000011304. [P26599-2]
GeneIDi5725.
KEGGihsa:5725.
UCSCiuc002lpp.3. human. [P26599-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Web resourcesi

Atlas of Genetics and Cytogenetics in Oncology and Haematology

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X62006 mRNA. Translation: CAA43973.1.
X65371 mRNA. Translation: CAA46443.1.
X65372 mRNA. Translation: CAA46444.1.
X60648 mRNA. Translation: CAA43056.1.
X66975 mRNA. Translation: CAA47386.1.
BT006819 mRNA. Translation: AAP35465.1.
AC006273 Genomic DNA. Translation: AAC99798.1.
CH471242 Genomic DNA. Translation: EAW61154.1.
BC002397 mRNA. Translation: AAH02397.1.
BC004383 mRNA. Translation: AAH04383.1.
BC013694 mRNA. Translation: AAH13694.1.
CCDSiCCDS32859.1. [P26599-1]
CCDS42456.1. [P26599-3]
CCDS45892.1. [P26599-2]
PIRiS23016.
RefSeqiNP_002810.1. NM_002819.4. [P26599-3]
NP_114367.1. NM_031990.3. [P26599-2]
NP_114368.1. NM_031991.3. [P26599-1]
UniGeneiHs.172550.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1QM9NMR-A335-531[»]
1SJQNMR-A55-147[»]
1SJRNMR-A147-301[»]
2AD9NMR-A49-146[»]
2ADBNMR-A172-298[»]
2ADCNMR-A324-531[»]
2EVZNMR-A324-531[»]
2N3ONMR-A41-163[»]
3ZZYX-ray1.40A/B156-285[»]
3ZZZX-ray1.55A/B156-285[»]
ProteinModelPortaliP26599.
SMRiP26599.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi111697. 127 interactors.
IntActiP26599. 48 interactors.
MINTiMINT-1348690.
STRINGi9606.ENSP00000349428.

Chemistry databases

ChEMBLiCHEMBL1293230.

PTM databases

iPTMnetiP26599.
PhosphoSitePlusiP26599.
SwissPalmiP26599.

Polymorphism and mutation databases

BioMutaiPTBP1.
DMDMi131528.

2D gel databases

SWISS-2DPAGEP26599.

Proteomic databases

EPDiP26599.
MaxQBiP26599.
PaxDbiP26599.
PeptideAtlasiP26599.
PRIDEiP26599.
TopDownProteomicsiP26599-1. [P26599-1]
P26599-2. [P26599-2]

Protocols and materials databases

DNASUi5725.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000349038; ENSP00000014112; ENSG00000011304. [P26599-1]
ENST00000356948; ENSP00000349428; ENSG00000011304. [P26599-3]
ENST00000394601; ENSP00000408096; ENSG00000011304. [P26599-2]
GeneIDi5725.
KEGGihsa:5725.
UCSCiuc002lpp.3. human. [P26599-1]

Organism-specific databases

CTDi5725.
DisGeNETi5725.
GeneCardsiPTBP1.
H-InvDBHIX0137476.
HGNCiHGNC:9583. PTBP1.
HPAiCAB013507.
MIMi600693. gene.
neXtProtiNX_P26599.
OpenTargetsiENSG00000011304.
PharmGKBiPA33934.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1190. Eukaryota.
ENOG410XPMZ. LUCA.
GeneTreeiENSGT00550000074508.
HOGENOMiHOG000231699.
HOVERGENiHBG069548.
InParanoidiP26599.
KOiK13218.
OMAiMSAQHAK.
OrthoDBiEOG091G0Z5R.
PhylomeDBiP26599.
TreeFamiTF319824.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000011304-MONOMER.
ReactomeiR-HSA-6803529. FGFR2 alternative splicing.
R-HSA-72163. mRNA Splicing - Major Pathway.
R-HSA-72203. Processing of Capped Intron-Containing Pre-mRNA.
SIGNORiP26599.

Miscellaneous databases

ChiTaRSiPTBP1. human.
EvolutionaryTraceiP26599.
GeneWikiiPTBP1.
GenomeRNAii5725.
PMAP-CutDBP26599.
PROiP26599.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000011304.
CleanExiHS_PTBP1.
ExpressionAtlasiP26599. baseline and differential.
GenevisibleiP26599. HS.

Family and domain databases

Gene3Di3.30.70.330. 4 hits.
InterProiIPR006536. HnRNP-L/PTB.
IPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PfamiPF00076. RRM_1. 2 hits.
[Graphical view]
SMARTiSM00360. RRM. 4 hits.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 4 hits.
TIGRFAMsiTIGR01649. hnRNP-L_PTB. 1 hit.
PROSITEiPS50102. RRM. 4 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPTBP1_HUMAN
AccessioniPrimary (citable) accession number: P26599
Secondary accession number(s): Q9BUQ0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: August 1, 1992
Last modified: November 30, 2016
This is version 194 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 19
    Human chromosome 19: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.