Reviewed,
UniProtKB/Swiss-Prot P26572 (MGAT1_HUMAN)
Last modified
January 19, 2010.
Version 94.
History...
Clusters with 100%,
90%,
50% identity |
Documents (6) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase EC=2.4.1.101 Alternative name(s): N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase I Short name=GlcNAc-T I Short name=GNT-I | ||||
| Gene names |
| ||||
| Organism | Homo sapiens (Human) [Complete proteome] | ||||
| Taxonomic identifier | 9606 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Protein attributes
| Sequence length | 445 AA. |
| Sequence status | Complete. |
| Protein existence | Evidence at transcript level. |
General annotation (Comments)
| Function | Initiates complex N-linked carbohydrate formation. Essential for the conversion of high-mannose to hybrid and complex N-glycans. |
| Catalytic activity | UDP-N-acetyl-D-glucosamine + 3-(alpha-D-mannosyl)-beta-D-mannosyl-R = UDP + 3-(2-(N-acetyl-beta-D-glucosaminyl)-alpha-D-mannosyl)-beta-D-mannosyl-R. |
| Pathway | |
| Subcellular location | Golgi apparatus membrane; Single-pass type II membrane protein. |
| Tissue specificity | Appears to be present in all tissues. |
| Sequence similarities | Belongs to the glycosyltransferase 13 family. |
Ontologies
| Keywords | |
|---|---|
| Cellular component | Golgi apparatus Membrane |
| Coding sequence diversity | Polymorphism |
| Domain | Signal-anchor Transmembrane |
| Molecular function | Glycosyltransferase Transferase |
| Technical term | Complete proteome |
| Gene Ontology (GO) | |
| Biological process | protein amino acid N-linked glycosylation Inferred from electronic annotation. Source: InterPro |
| Cellular component | Golgi membrane Inferred from electronic annotation. Source: InterPro integral to membrane Ref.2Traceable author statement. Source: ProtInc |
| Molecular function | alpha-1,3-mannosylglycoprotein 2-beta-N-acetylglucosaminyltransferase activity Inferred from electronic annotation. Source: EC |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 445 | 445 | Alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase | PRO_0000191384 | |||||
Regions | |||||||||
| Topological domain | 1 – 6 | 6 | Cytoplasmic Potential | ||||||
| Transmembrane | 7 – 29 | 23 | Signal-anchor for type II membrane protein Potential | ||||||
| Topological domain | 30 – 445 | 416 | Lumenal Potential | ||||||
Natural variations | |||||||||
| Natural variant | 223 | 1 | R → Q: dbSNP rs7726005. | VAR_028272 | |||||
| Natural variant | 435 | 1 | P → L: dbSNP rs634501. Ref.2 Ref.3 Ref.5 | VAR_028273 | |||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Organization and localization to chromosome 5 of the human UDP-N-acetylglucosamine:alpha-3-D-mannoside beta-1,2-N-acetylglucosaminyltransferase I gene." Hull E., Sarkar M., Spruijt M.P.N., Hoeppener J.W.M., Dunn R., Schachter H. Biochem. Biophys. Res. Commun. 176:608-615(1991) [PubMed: 1827260] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Tissue: Myeloid leukemia cell. |
| [2] | "Cloning and expression of N-acetylglucosaminyltransferase I, the medial Golgi transferase that initiates complex N-linked carbohydrate formation." Kumar R., Yang J., Larsen R.D., Stanley P. Proc. Natl. Acad. Sci. U.S.A. 87:9948-9952(1990) [PubMed: 1702225] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT LEU-435. |
| [3] | "Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. Sugano S.Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT LEU-435. |
| [4] | "Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)." Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B. Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. |
| [5] | Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. Venter J.C.Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], VARIANT LEU-435. |
| [6] | "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Kidney. |
| + | Additional computationally mapped references. |
Web resources
| GGDB GlycoGene database |
| Functional Glycomics Gateway - GTase Alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | M61829 Genomic DNA. Translation: AAA75523.1. M55621 mRNA. Translation: AAA52563.1. AK094130 mRNA. Translation: BAG52821.1. AK290769 mRNA. Translation: BAF83458.1. CR456861 mRNA. Translation: CAG33142.1. CH471165 Genomic DNA. Translation: EAW53739.1. BC003575 mRNA. Translation: AAH03575.1. |
| IPI | IPI00000138. |
| PIR | XUHUMB. JH0397. |
| RefSeq | NP_001108089.1. NP_001108090.1. NP_001108091.1. NP_001108092.1. NP_002397.2. |
| UniGene | Hs.519818 |
3D structure databases | |
| SMR | P26572. Positions 104-444. |
| ModBase | Search... |
Protein-protein interaction databases | |
| STRING | P26572. |
Protein family/group databases | |
| CAZy | GT13. Glycosyltransferase Family 13. |
PTM databases | |
| PhosphoSite | P26572. |
Proteomic databases | |
| PRIDE | P26572. |
Genome annotation databases | |
| Ensembl | ENST00000307826; ENSP00000311888; ENSG00000131446; Homo sapiens. [Genome view] ENST00000333055; ENSP00000332073; ENSG00000131446; Homo sapiens. [Genome view] ENST00000393340; ENSP00000377010; ENSG00000131446; Homo sapiens. [Genome view] ENST00000427865; ENSP00000402838; ENSG00000131446; Homo sapiens. [Genome view] ENST00000446023; ENSP00000404718; ENSG00000131446; Homo sapiens. [Genome view] ENST00000452920; ENSP00000412647; ENSG00000131446; Homo sapiens. [Genome view] |
| GeneID | 4245. |
| KEGG | hsa:4245. |
Organism-specific databases | |
| CTD | 4245. |
| GeneCards | GC05M180150. |
| H-InvDB | HIX0005500. |
| HGNC | HGNC:7044. MGAT1. |
| HPA | HPA017432. |
| MIM | 160995. gene. |
| PharmGKB | PA30779. |
| GenAtlas | Search... |
Phylogenomic databases | |
| eggNOG | prNOG18878. |
| HOVERGEN | P26572. |
| InParanoid | P26572. |
Enzyme and pathway databases | |
| BRENDA | 2.4.1.101. 247. |
Gene expression databases | |
| ArrayExpress | P26572. |
| Bgee | P26572. |
| CleanEx | HS_MGAT1. |
| Genevestigator | P26572. |
| GermOnline | ENSG00000131446. Homo sapiens. |
Family and domain databases | |
| InterPro | IPR004139. Glyco_trans_13. [Graphical view] |
| PANTHER | PTHR10468. Glyco_trans_13. 1 hit. |
| Pfam | PF03071. GNT-I. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other Resources | |
| NextBio | 16733. |
| SOURCE | Search... |
Entry information
| Entry name | MGAT1_HUMAN | ||||||||
| Accession | Primary (citable) accession number: P26572 Secondary accession number(s): A8K404, Q6IBE3 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
| Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. | ||||||||
Relevant documents
| Human chromosome 5 Human chromosome 5: entries, gene names and cross-references to MIM |
| Human entries with polymorphisms or disease mutations List of human entries with polymorphisms or disease mutations |
| Human polymorphisms and disease mutations Index of human polymorphisms and disease mutations |
| MIM cross-references Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot |
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| SIMILARITY comments Index of protein domains and families |

Clusters with


