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P26570

- PPZ1_YEAST

UniProt

P26570 - PPZ1_YEAST

Protein

Serine/threonine-protein phosphatase PP-Z1

Gene

PPZ1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 146 (01 Oct 2014)
      Sequence version 5 (05 Oct 2010)
      Previous versions | rss
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    Functioni

    Essential for the maintenance of cell size and integrity in response to osmotic stress.

    Catalytic activityi

    [a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

    Cofactori

    Binds 2 manganese ions per subunit.By similarity

    Enzyme regulationi

    Inhibited by the regulatory subunits VHS3 and SIS2.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi419 – 4191Manganese 1By similarity
    Metal bindingi421 – 4211Manganese 1By similarity
    Metal bindingi447 – 4471Manganese 1By similarity
    Metal bindingi447 – 4471Manganese 2By similarity
    Metal bindingi479 – 4791Manganese 2By similarity
    Active sitei480 – 4801Proton donorBy similarity
    Metal bindingi528 – 5281Manganese 2By similarity
    Metal bindingi603 – 6031Manganese 2By similarity

    GO - Molecular functioni

    1. cofactor binding Source: InterPro
    2. magnesium-dependent protein serine/threonine phosphatase activity Source: InterPro
    3. metal ion binding Source: UniProtKB-KW
    4. protein binding Source: IntAct
    5. protein serine/threonine phosphatase activity Source: SGD

    GO - Biological processi

    1. cellular protein localization Source: SGD
    2. cellular sodium ion homeostasis Source: SGD
    3. protein dephosphorylation Source: SGD

    Keywords - Molecular functioni

    Hydrolase, Protein phosphatase

    Keywords - Ligandi

    Manganese, Metal-binding

    Enzyme and pathway databases

    BioCyciYEAST:G3O-32620-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Serine/threonine-protein phosphatase PP-Z1 (EC:3.1.3.16)
    Gene namesi
    Name:PPZ1
    Ordered Locus Names:YML016C
    ORF Names:YM9571.02C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XIII

    Organism-specific databases

    SGDiS000004478. PPZ1.

    Subcellular locationi

    GO - Cellular componenti

    1. extrinsic component of plasma membrane Source: SGD
    2. nucleus Source: SGD

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedSequence Analysis
    Chaini2 – 692691Serine/threonine-protein phosphatase PP-Z1PRO_0000058891Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Lipidationi2 – 21N-myristoyl glycineSequence Analysis
    Modified residuei49 – 491Phosphoserine1 Publication
    Modified residuei171 – 1711Phosphothreonine1 Publication
    Modified residuei222 – 2221Phosphoserine1 Publication
    Modified residuei261 – 2611Phosphothreonine1 Publication
    Modified residuei265 – 2651Phosphoserine1 Publication
    Modified residuei690 – 6901Phosphoserine1 Publication

    Keywords - PTMi

    Lipoprotein, Myristate, Phosphoprotein

    Proteomic databases

    MaxQBiP26570.
    PaxDbiP26570.
    PeptideAtlasiP26570.
    PRIDEiP26570.

    Expressioni

    Gene expression databases

    GenevestigatoriP26570.

    Interactioni

    Subunit structurei

    Interacts with SIS2 and VHS3, which regulate its activity.2 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    CAB3P360766EBI-13807,EBI-26778
    SIS2P360245EBI-13807,EBI-17250
    VHS3Q084384EBI-13807,EBI-30482

    Protein-protein interaction databases

    BioGridi35153. 191 interactions.
    DIPiDIP-557N.
    IntActiP26570. 43 interactions.
    MINTiMINT-709853.
    STRINGi4932.YML016C.

    Structurei

    3D structure databases

    ProteinModelPortaliP26570.
    SMRiP26570. Positions 361-653.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi2 – 319318Ser-richAdd
    BLAST

    Sequence similaritiesi

    Belongs to the PPP phosphatase family. PP-Z subfamily.Curated

    Phylogenomic databases

    eggNOGiCOG0639.
    GeneTreeiENSGT00710000107291.
    HOGENOMiHOG000172697.
    KOiK06269.
    OMAiYYENALT.
    OrthoDBiEOG79KPQ9.

    Family and domain databases

    Gene3Di3.60.21.10. 1 hit.
    InterProiIPR004843. Calcineurin-like_PHP_apaH.
    IPR029052. Metallo-depent_PP-like.
    IPR011159. PPPtase_PPZ.
    IPR006186. Ser/Thr-sp_prot-phosphatase.
    [Graphical view]
    PfamiPF00149. Metallophos. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000909. PPPtase_PPZ. 1 hit.
    PRINTSiPR00114. STPHPHTASE.
    SMARTiSM00156. PP2Ac. 1 hit.
    [Graphical view]
    SUPFAMiSSF56300. SSF56300. 1 hit.
    PROSITEiPS00125. SER_THR_PHOSPHATASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P26570-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGNSSSKSSK KDSHSNSSSR NPRPQVSRTE TSHSVKSAKS NKSSRSRRSL    50
    PSSSTTNTNS NVPDPSTPSK PNLEVNHQRH SSHTNRYHFP SSSHSHSNSQ 100
    NELLTTPSSS STKRPSTSRR SSYNTKAAAD LPPSMIQMEP KSPILKTNNS 150
    STHVSKHKSS YSSTYYENAL TDDDNDDKDN DISHTKRFSR SSNSRPSSIR 200
    SGSVSRRKSD VTHEEPNNGS YSSNNQENYL VQALTRSNSH ASSLHSRKSS 250
    FGSDGNTAYS TPLNSPGLSK LTDHSGEYFT SNSTSSLNHH SSRDIYPSKH 300
    ISNDDDIENS SQLSNIHASM ENVNDKNNNI TDSKKDPNEE FNDIMQSSGN 350
    KNAPKKFKKP IDIDETIQKL LDAGYAAKRT KNVCLKNNEI LQICIKAREI 400
    FLSQPSLLEL SPPVKIVGDV HGQYGDLLRL FTKCGFPPSS NYLFLGDYVD 450
    RGKQSLETIL LLFCYKIKYP ENFFLLRGNH ECANVTRVYG FYDECKRRCN 500
    IKIWKTFIDT FNTLPLAAIV AGKIFCVHGG LSPVLNSMDE IRHVVRPTDV 550
    PDFGLINDLL WSDPTDSPNE WEDNERGVSY CYNKVAINKF LNKFGFDLVC 600
    RAHMVVEDGY EFFNDRSLVT VFSAPNYCGE FDNWGAVMSV SEGLLCSFEL 650
    LDPLDSAALK QVMKKGRQER KLANQQQQMM ETSITNDNES QQ 692
    Length:692
    Mass (Da):77,491
    Last modified:October 5, 2010 - v5
    Checksum:i8B7F009521F72C15
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti340 – 3401E → G in AAA34898. (PubMed:1318299)Curated
    Sequence conflicti440 – 4401S → A no nucleotide entry (PubMed:2166691)Curated
    Sequence conflicti649 – 6491E → K no nucleotide entry (PubMed:2166691)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M86242 Genomic DNA. Translation: AAA34898.1.
    X74135 Genomic DNA. Translation: CAA52232.1.
    Z49810 Genomic DNA. Translation: CAA89936.1.
    BK006946 Genomic DNA. Translation: DAA09882.1.
    PIRiS55103. PABY12.
    RefSeqiNP_013696.1. NM_001182374.1.

    Genome annotation databases

    EnsemblFungiiYML016C; YML016C; YML016C.
    GeneIDi854992.
    KEGGisce:YML016C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M86242 Genomic DNA. Translation: AAA34898.1 .
    X74135 Genomic DNA. Translation: CAA52232.1 .
    Z49810 Genomic DNA. Translation: CAA89936.1 .
    BK006946 Genomic DNA. Translation: DAA09882.1 .
    PIRi S55103. PABY12.
    RefSeqi NP_013696.1. NM_001182374.1.

    3D structure databases

    ProteinModelPortali P26570.
    SMRi P26570. Positions 361-653.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 35153. 191 interactions.
    DIPi DIP-557N.
    IntActi P26570. 43 interactions.
    MINTi MINT-709853.
    STRINGi 4932.YML016C.

    Proteomic databases

    MaxQBi P26570.
    PaxDbi P26570.
    PeptideAtlasi P26570.
    PRIDEi P26570.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YML016C ; YML016C ; YML016C .
    GeneIDi 854992.
    KEGGi sce:YML016C.

    Organism-specific databases

    SGDi S000004478. PPZ1.

    Phylogenomic databases

    eggNOGi COG0639.
    GeneTreei ENSGT00710000107291.
    HOGENOMi HOG000172697.
    KOi K06269.
    OMAi YYENALT.
    OrthoDBi EOG79KPQ9.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-32620-MONOMER.

    Miscellaneous databases

    NextBioi 978133.

    Gene expression databases

    Genevestigatori P26570.

    Family and domain databases

    Gene3Di 3.60.21.10. 1 hit.
    InterProi IPR004843. Calcineurin-like_PHP_apaH.
    IPR029052. Metallo-depent_PP-like.
    IPR011159. PPPtase_PPZ.
    IPR006186. Ser/Thr-sp_prot-phosphatase.
    [Graphical view ]
    Pfami PF00149. Metallophos. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000909. PPPtase_PPZ. 1 hit.
    PRINTSi PR00114. STPHPHTASE.
    SMARTi SM00156. PP2Ac. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56300. SSF56300. 1 hit.
    PROSITEi PS00125. SER_THR_PHOSPHATASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning and analysis of a yeast protein phosphatase with an unusual amino-terminal region."
      Posas F., Casamayor A., Morral N., Arino J.
      J. Biol. Chem. 267:11734-11740(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: M5.
    2. "Both isoforms of protein phosphatase Z are essential for the maintenance of cell size and integrity in Saccharomyces cerevisiae in response to osmotic stress."
      Hughes V., Mueller A., Stark M.J.R., Cohen P.T.W.
      Eur. J. Biochem. 216:269-279(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: AY926.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    5. "Protein serine/threonine phosphatases; an expanding family."
      Cohen P.T.W., Brewis N.D., Hughes V., Mann D.J.
      FEBS Lett. 268:355-359(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 345-692.
    6. "Protein phosphatase 2Bw and protein phosphatase Z are Saccharomyces cerevisiae enzymes."
      Da Cruz e Silva E.F., Hughes V., McDonald P., Stark M.J.R., Cohen P.T.W.
      Biochim. Biophys. Acta 1089:269-272(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 389-610.
    7. "The yeast halotolerance determinant Hal3p is an inhibitory subunit of the Ppz1p Ser/Thr protein phosphatase."
      de Nadal E., Clotet J., Posas F., Serrano R., Gomez N., Arino J.
      Proc. Natl. Acad. Sci. U.S.A. 95:7357-7362(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH SIS2.
    8. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    9. "Functional characterization of the Saccharomyces cerevisiae VHS3 gene. A regulatory subunit of the Ppz1 protein phosphatase with novel, phosphatase-unrelated functions."
      Ruiz A., Munoz I., Serrano R., Gonzalez A., Simon E., Arino J.
      J. Biol. Chem. 279:34421-34430(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH VHS3.
    10. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
      Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
      J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-49; THR-171; THR-261 AND SER-265, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: ADR376.
    11. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-222 AND SER-690, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiPPZ1_YEAST
    AccessioniPrimary (citable) accession number: P26570
    Secondary accession number(s): D6VZF8, Q00979
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1992
    Last sequence update: October 5, 2010
    Last modified: October 1, 2014
    This is version 146 of the entry and version 5 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 217 molecules/cell in log phase SD medium.1 Publication

    Caution

    Was originally thought to originate from a rabbit cDNA library and was known as protein phosphatase Z (PP-Z).1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome XIII
      Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

    External Data

    Dasty 3