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P26570

- PPZ1_YEAST

UniProt

P26570 - PPZ1_YEAST

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Protein

Serine/threonine-protein phosphatase PP-Z1

Gene

PPZ1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Essential for the maintenance of cell size and integrity in response to osmotic stress.

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactori

Mn2+By similarityNote: Binds 2 manganese ions per subunit.By similarity

Enzyme regulationi

Inhibited by the regulatory subunits VHS3 and SIS2.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi419 – 4191Manganese 1By similarity
Metal bindingi421 – 4211Manganese 1By similarity
Metal bindingi447 – 4471Manganese 1By similarity
Metal bindingi447 – 4471Manganese 2By similarity
Metal bindingi479 – 4791Manganese 2By similarity
Active sitei480 – 4801Proton donorBy similarity
Metal bindingi528 – 5281Manganese 2By similarity
Metal bindingi603 – 6031Manganese 2By similarity

GO - Molecular functioni

  1. cofactor binding Source: InterPro
  2. magnesium-dependent protein serine/threonine phosphatase activity Source: InterPro
  3. metal ion binding Source: UniProtKB-KW
  4. protein serine/threonine phosphatase activity Source: SGD

GO - Biological processi

  1. cellular protein localization Source: SGD
  2. cellular sodium ion homeostasis Source: SGD
  3. protein dephosphorylation Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Keywords - Ligandi

Manganese, Metal-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-32620-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein phosphatase PP-Z1 (EC:3.1.3.16)
Gene namesi
Name:PPZ1
Ordered Locus Names:YML016C
ORF Names:YM9571.02C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XIII

Organism-specific databases

SGDiS000004478. PPZ1.

Subcellular locationi

GO - Cellular componenti

  1. extrinsic component of plasma membrane Source: SGD
  2. nucleus Source: SGD
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedSequence Analysis
Chaini2 – 692691Serine/threonine-protein phosphatase PP-Z1PRO_0000058891Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi2 – 21N-myristoyl glycineSequence Analysis
Modified residuei49 – 491Phosphoserine1 Publication
Modified residuei171 – 1711Phosphothreonine1 Publication
Modified residuei222 – 2221Phosphoserine1 Publication
Modified residuei261 – 2611Phosphothreonine1 Publication
Modified residuei265 – 2651Phosphoserine1 Publication
Modified residuei690 – 6901Phosphoserine1 Publication

Keywords - PTMi

Lipoprotein, Myristate, Phosphoprotein

Proteomic databases

MaxQBiP26570.
PaxDbiP26570.
PeptideAtlasiP26570.
PRIDEiP26570.

Expressioni

Gene expression databases

GenevestigatoriP26570.

Interactioni

Subunit structurei

Interacts with SIS2 and VHS3, which regulate its activity.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
CAB3P360766EBI-13807,EBI-26778
SIS2P360245EBI-13807,EBI-17250
VHS3Q084384EBI-13807,EBI-30482

Protein-protein interaction databases

BioGridi35153. 191 interactions.
DIPiDIP-557N.
IntActiP26570. 43 interactions.
MINTiMINT-709853.
STRINGi4932.YML016C.

Structurei

3D structure databases

ProteinModelPortaliP26570.
SMRiP26570. Positions 361-653.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi2 – 319318Ser-richAdd
BLAST

Sequence similaritiesi

Belongs to the PPP phosphatase family. PP-Z subfamily.Curated

Phylogenomic databases

eggNOGiCOG0639.
GeneTreeiENSGT00710000107291.
HOGENOMiHOG000172697.
InParanoidiP26570.
KOiK06269.
OMAiYYENALT.
OrthoDBiEOG79KPQ9.

Family and domain databases

Gene3Di3.60.21.10. 1 hit.
InterProiIPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
IPR011159. PPPtase_PPZ.
IPR006186. Ser/Thr-sp_prot-phosphatase.
[Graphical view]
PfamiPF00149. Metallophos. 1 hit.
[Graphical view]
PIRSFiPIRSF000909. PPPtase_PPZ. 1 hit.
PRINTSiPR00114. STPHPHTASE.
SMARTiSM00156. PP2Ac. 1 hit.
[Graphical view]
SUPFAMiSSF56300. SSF56300. 1 hit.
PROSITEiPS00125. SER_THR_PHOSPHATASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P26570-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MGNSSSKSSK KDSHSNSSSR NPRPQVSRTE TSHSVKSAKS NKSSRSRRSL
60 70 80 90 100
PSSSTTNTNS NVPDPSTPSK PNLEVNHQRH SSHTNRYHFP SSSHSHSNSQ
110 120 130 140 150
NELLTTPSSS STKRPSTSRR SSYNTKAAAD LPPSMIQMEP KSPILKTNNS
160 170 180 190 200
STHVSKHKSS YSSTYYENAL TDDDNDDKDN DISHTKRFSR SSNSRPSSIR
210 220 230 240 250
SGSVSRRKSD VTHEEPNNGS YSSNNQENYL VQALTRSNSH ASSLHSRKSS
260 270 280 290 300
FGSDGNTAYS TPLNSPGLSK LTDHSGEYFT SNSTSSLNHH SSRDIYPSKH
310 320 330 340 350
ISNDDDIENS SQLSNIHASM ENVNDKNNNI TDSKKDPNEE FNDIMQSSGN
360 370 380 390 400
KNAPKKFKKP IDIDETIQKL LDAGYAAKRT KNVCLKNNEI LQICIKAREI
410 420 430 440 450
FLSQPSLLEL SPPVKIVGDV HGQYGDLLRL FTKCGFPPSS NYLFLGDYVD
460 470 480 490 500
RGKQSLETIL LLFCYKIKYP ENFFLLRGNH ECANVTRVYG FYDECKRRCN
510 520 530 540 550
IKIWKTFIDT FNTLPLAAIV AGKIFCVHGG LSPVLNSMDE IRHVVRPTDV
560 570 580 590 600
PDFGLINDLL WSDPTDSPNE WEDNERGVSY CYNKVAINKF LNKFGFDLVC
610 620 630 640 650
RAHMVVEDGY EFFNDRSLVT VFSAPNYCGE FDNWGAVMSV SEGLLCSFEL
660 670 680 690
LDPLDSAALK QVMKKGRQER KLANQQQQMM ETSITNDNES QQ
Length:692
Mass (Da):77,491
Last modified:October 5, 2010 - v5
Checksum:i8B7F009521F72C15
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti340 – 3401E → G in AAA34898. (PubMed:1318299)Curated
Sequence conflicti440 – 4401S → A no nucleotide entry (PubMed:2166691)Curated
Sequence conflicti649 – 6491E → K no nucleotide entry (PubMed:2166691)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M86242 Genomic DNA. Translation: AAA34898.1.
X74135 Genomic DNA. Translation: CAA52232.1.
Z49810 Genomic DNA. Translation: CAA89936.1.
BK006946 Genomic DNA. Translation: DAA09882.1.
PIRiS55103. PABY12.
RefSeqiNP_013696.1. NM_001182374.1.

Genome annotation databases

EnsemblFungiiYML016C; YML016C; YML016C.
GeneIDi854992.
KEGGisce:YML016C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M86242 Genomic DNA. Translation: AAA34898.1 .
X74135 Genomic DNA. Translation: CAA52232.1 .
Z49810 Genomic DNA. Translation: CAA89936.1 .
BK006946 Genomic DNA. Translation: DAA09882.1 .
PIRi S55103. PABY12.
RefSeqi NP_013696.1. NM_001182374.1.

3D structure databases

ProteinModelPortali P26570.
SMRi P26570. Positions 361-653.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 35153. 191 interactions.
DIPi DIP-557N.
IntActi P26570. 43 interactions.
MINTi MINT-709853.
STRINGi 4932.YML016C.

Proteomic databases

MaxQBi P26570.
PaxDbi P26570.
PeptideAtlasi P26570.
PRIDEi P26570.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YML016C ; YML016C ; YML016C .
GeneIDi 854992.
KEGGi sce:YML016C.

Organism-specific databases

SGDi S000004478. PPZ1.

Phylogenomic databases

eggNOGi COG0639.
GeneTreei ENSGT00710000107291.
HOGENOMi HOG000172697.
InParanoidi P26570.
KOi K06269.
OMAi YYENALT.
OrthoDBi EOG79KPQ9.

Enzyme and pathway databases

BioCyci YEAST:G3O-32620-MONOMER.

Miscellaneous databases

NextBioi 978133.

Gene expression databases

Genevestigatori P26570.

Family and domain databases

Gene3Di 3.60.21.10. 1 hit.
InterProi IPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
IPR011159. PPPtase_PPZ.
IPR006186. Ser/Thr-sp_prot-phosphatase.
[Graphical view ]
Pfami PF00149. Metallophos. 1 hit.
[Graphical view ]
PIRSFi PIRSF000909. PPPtase_PPZ. 1 hit.
PRINTSi PR00114. STPHPHTASE.
SMARTi SM00156. PP2Ac. 1 hit.
[Graphical view ]
SUPFAMi SSF56300. SSF56300. 1 hit.
PROSITEi PS00125. SER_THR_PHOSPHATASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning and analysis of a yeast protein phosphatase with an unusual amino-terminal region."
    Posas F., Casamayor A., Morral N., Arino J.
    J. Biol. Chem. 267:11734-11740(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: M5.
  2. "Both isoforms of protein phosphatase Z are essential for the maintenance of cell size and integrity in Saccharomyces cerevisiae in response to osmotic stress."
    Hughes V., Mueller A., Stark M.J.R., Cohen P.T.W.
    Eur. J. Biochem. 216:269-279(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: AY926.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. "Protein serine/threonine phosphatases; an expanding family."
    Cohen P.T.W., Brewis N.D., Hughes V., Mann D.J.
    FEBS Lett. 268:355-359(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 345-692.
  6. "Protein phosphatase 2Bw and protein phosphatase Z are Saccharomyces cerevisiae enzymes."
    Da Cruz e Silva E.F., Hughes V., McDonald P., Stark M.J.R., Cohen P.T.W.
    Biochim. Biophys. Acta 1089:269-272(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 389-610.
  7. "The yeast halotolerance determinant Hal3p is an inhibitory subunit of the Ppz1p Ser/Thr protein phosphatase."
    de Nadal E., Clotet J., Posas F., Serrano R., Gomez N., Arino J.
    Proc. Natl. Acad. Sci. U.S.A. 95:7357-7362(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH SIS2.
  8. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  9. "Functional characterization of the Saccharomyces cerevisiae VHS3 gene. A regulatory subunit of the Ppz1 protein phosphatase with novel, phosphatase-unrelated functions."
    Ruiz A., Munoz I., Serrano R., Gonzalez A., Simon E., Arino J.
    J. Biol. Chem. 279:34421-34430(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH VHS3.
  10. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-49; THR-171; THR-261 AND SER-265, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  11. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-222 AND SER-690, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiPPZ1_YEAST
AccessioniPrimary (citable) accession number: P26570
Secondary accession number(s): D6VZF8, Q00979
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: October 5, 2010
Last modified: November 26, 2014
This is version 148 of the entry and version 5 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 217 molecules/cell in log phase SD medium.1 Publication

Caution

Was originally thought to originate from a rabbit cDNA library and was known as protein phosphatase Z (PP-Z).1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

External Data

Dasty 3