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Protein

Serine/threonine-protein phosphatase PP-Z1

Gene

PPZ1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Essential for the maintenance of cell size and integrity in response to osmotic stress.

Miscellaneous

Present with 217 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactori

Mn2+By similarityNote: Binds 2 manganese ions per subunit.By similarity

Enzyme regulationi

Inhibited by the regulatory subunits VHS3 and SIS2.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi419Manganese 1By similarity1
Metal bindingi421Manganese 1By similarity1
Metal bindingi447Manganese 1By similarity1
Metal bindingi447Manganese 2By similarity1
Metal bindingi479Manganese 2By similarity1
Active sitei480Proton donorBy similarity1
Metal bindingi528Manganese 2By similarity1
Metal bindingi603Manganese 2By similarity1

GO - Molecular functioni

GO - Biological processi

  • cellular protein localization Source: SGD
  • cellular sodium ion homeostasis Source: SGD
  • protein dephosphorylation Source: SGD

Keywordsi

Molecular functionHydrolase, Protein phosphatase
LigandManganese, Metal-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-32620-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein phosphatase PP-Z1 (EC:3.1.3.16)
Gene namesi
Name:PPZ1
Ordered Locus Names:YML016C
ORF Names:YM9571.02C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIII

Organism-specific databases

EuPathDBiFungiDB:YML016C.
SGDiS000004478. PPZ1.

Subcellular locationi

GO - Cellular componenti

  • extrinsic component of plasma membrane Source: SGD
  • nucleus Source: SGD

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedSequence analysis
ChainiPRO_00000588912 – 692Serine/threonine-protein phosphatase PP-Z1Add BLAST691

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi2N-myristoyl glycineSequence analysis1
Modified residuei49PhosphoserineCombined sources1
Modified residuei171PhosphothreonineCombined sources1
Modified residuei209PhosphoserineBy similarity1
Modified residuei222PhosphoserineCombined sources1
Modified residuei261PhosphothreonineCombined sources1
Modified residuei265PhosphoserineCombined sources1
Modified residuei690PhosphoserineCombined sources1

Keywords - PTMi

Lipoprotein, Myristate, Phosphoprotein

Proteomic databases

MaxQBiP26570.
PRIDEiP26570.

PTM databases

iPTMnetiP26570.

Interactioni

Subunit structurei

Interacts with SIS2 and VHS3, which regulate its activity.2 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi35153. 307 interactors.
DIPiDIP-557N.
IntActiP26570. 44 interactors.
MINTiMINT-709853.

Structurei

3D structure databases

ProteinModelPortaliP26570.
SMRiP26570.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi2 – 319Ser-richAdd BLAST318

Sequence similaritiesi

Belongs to the PPP phosphatase family. PP-Z subfamily.Curated

Phylogenomic databases

GeneTreeiENSGT00840000130448.
HOGENOMiHOG000172697.
InParanoidiP26570.
KOiK06269.
OMAiCHLAREI.
OrthoDBiEOG092C37EX.

Family and domain databases

Gene3Di3.60.21.10. 1 hit.
InterProiView protein in InterPro
IPR004843. Calcineurin-like_PHP_ApaH.
IPR029052. Metallo-depent_PP-like.
IPR011159. PPPtase_PPZ/Ppq1.
IPR006186. Ser/Thr-sp_prot-phosphatase.
IPR031675. STPPase_N.
PfamiView protein in Pfam
PF00149. Metallophos. 1 hit.
PF16891. STPPase_N. 1 hit.
PIRSFiPIRSF000909. PPPtase_PPZ. 1 hit.
PRINTSiPR00114. STPHPHTASE.
SMARTiView protein in SMART
SM00156. PP2Ac. 1 hit.
SUPFAMiSSF56300. SSF56300. 1 hit.
PROSITEiView protein in PROSITE
PS00125. SER_THR_PHOSPHATASE. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P26570-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGNSSSKSSK KDSHSNSSSR NPRPQVSRTE TSHSVKSAKS NKSSRSRRSL
60 70 80 90 100
PSSSTTNTNS NVPDPSTPSK PNLEVNHQRH SSHTNRYHFP SSSHSHSNSQ
110 120 130 140 150
NELLTTPSSS STKRPSTSRR SSYNTKAAAD LPPSMIQMEP KSPILKTNNS
160 170 180 190 200
STHVSKHKSS YSSTYYENAL TDDDNDDKDN DISHTKRFSR SSNSRPSSIR
210 220 230 240 250
SGSVSRRKSD VTHEEPNNGS YSSNNQENYL VQALTRSNSH ASSLHSRKSS
260 270 280 290 300
FGSDGNTAYS TPLNSPGLSK LTDHSGEYFT SNSTSSLNHH SSRDIYPSKH
310 320 330 340 350
ISNDDDIENS SQLSNIHASM ENVNDKNNNI TDSKKDPNEE FNDIMQSSGN
360 370 380 390 400
KNAPKKFKKP IDIDETIQKL LDAGYAAKRT KNVCLKNNEI LQICIKAREI
410 420 430 440 450
FLSQPSLLEL SPPVKIVGDV HGQYGDLLRL FTKCGFPPSS NYLFLGDYVD
460 470 480 490 500
RGKQSLETIL LLFCYKIKYP ENFFLLRGNH ECANVTRVYG FYDECKRRCN
510 520 530 540 550
IKIWKTFIDT FNTLPLAAIV AGKIFCVHGG LSPVLNSMDE IRHVVRPTDV
560 570 580 590 600
PDFGLINDLL WSDPTDSPNE WEDNERGVSY CYNKVAINKF LNKFGFDLVC
610 620 630 640 650
RAHMVVEDGY EFFNDRSLVT VFSAPNYCGE FDNWGAVMSV SEGLLCSFEL
660 670 680 690
LDPLDSAALK QVMKKGRQER KLANQQQQMM ETSITNDNES QQ
Length:692
Mass (Da):77,491
Last modified:October 5, 2010 - v5
Checksum:i8B7F009521F72C15
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti340E → G in AAA34898 (PubMed:1318299).Curated1
Sequence conflicti440S → A no nucleotide entry (PubMed:2166691).Curated1
Sequence conflicti649E → K no nucleotide entry (PubMed:2166691).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M86242 Genomic DNA. Translation: AAA34898.1.
X74135 Genomic DNA. Translation: CAA52232.1.
Z49810 Genomic DNA. Translation: CAA89936.1.
BK006946 Genomic DNA. Translation: DAA09882.1.
PIRiS55103. PABY12.
RefSeqiNP_013696.1. NM_001182374.1.

Genome annotation databases

EnsemblFungiiYML016C; YML016C; YML016C.
GeneIDi854992.
KEGGisce:YML016C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M86242 Genomic DNA. Translation: AAA34898.1.
X74135 Genomic DNA. Translation: CAA52232.1.
Z49810 Genomic DNA. Translation: CAA89936.1.
BK006946 Genomic DNA. Translation: DAA09882.1.
PIRiS55103. PABY12.
RefSeqiNP_013696.1. NM_001182374.1.

3D structure databases

ProteinModelPortaliP26570.
SMRiP26570.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35153. 307 interactors.
DIPiDIP-557N.
IntActiP26570. 44 interactors.
MINTiMINT-709853.

PTM databases

iPTMnetiP26570.

Proteomic databases

MaxQBiP26570.
PRIDEiP26570.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYML016C; YML016C; YML016C.
GeneIDi854992.
KEGGisce:YML016C.

Organism-specific databases

EuPathDBiFungiDB:YML016C.
SGDiS000004478. PPZ1.

Phylogenomic databases

GeneTreeiENSGT00840000130448.
HOGENOMiHOG000172697.
InParanoidiP26570.
KOiK06269.
OMAiCHLAREI.
OrthoDBiEOG092C37EX.

Enzyme and pathway databases

BioCyciYEAST:G3O-32620-MONOMER.

Miscellaneous databases

PROiPR:P26570.

Family and domain databases

Gene3Di3.60.21.10. 1 hit.
InterProiView protein in InterPro
IPR004843. Calcineurin-like_PHP_ApaH.
IPR029052. Metallo-depent_PP-like.
IPR011159. PPPtase_PPZ/Ppq1.
IPR006186. Ser/Thr-sp_prot-phosphatase.
IPR031675. STPPase_N.
PfamiView protein in Pfam
PF00149. Metallophos. 1 hit.
PF16891. STPPase_N. 1 hit.
PIRSFiPIRSF000909. PPPtase_PPZ. 1 hit.
PRINTSiPR00114. STPHPHTASE.
SMARTiView protein in SMART
SM00156. PP2Ac. 1 hit.
SUPFAMiSSF56300. SSF56300. 1 hit.
PROSITEiView protein in PROSITE
PS00125. SER_THR_PHOSPHATASE. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiPPZ1_YEAST
AccessioniPrimary (citable) accession number: P26570
Secondary accession number(s): D6VZF8, Q00979
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: October 5, 2010
Last modified: March 15, 2017
This is version 169 of the entry and version 5 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Caution

Was originally thought to originate from a rabbit cDNA library and was known as protein phosphatase Z (PP-Z).1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.