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P26562

- HEMA_I76A4

UniProt

P26562 - HEMA_I76A4

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Protein
Hemagglutinin
Gene
HA
Organism
Influenza A virus (strain A/Duck/Alberta/35/1976 H1N1)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Binds to sialic acid-containing receptors on the cell surface, bringing about the attachment of the virus particle to the cell. This attachment induces virion internalization of about two third of the virus particles through clathrin-dependent endocytosis and about one third through a clathrin- and caveolin-independent pathway. Plays a major role in the determination of host range restriction and virulence. Class I viral fusion protein. Responsible for penetration of the virus into the cell cytoplasm by mediating the fusion of the membrane of the endocytosed virus particle with the endosomal membrane. Low pH in endosomes induces an irreversible conformational change in HA2, releasing the fusion hydrophobic peptide. Several trimers are required to form a competent fusion pore.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei344 – 3452Cleavage; by host By similarity

GO - Biological processi

  1. clathrin-mediated endocytosis of virus by host cell Source: UniProtKB-KW
  2. fusion of virus membrane with host endosome membrane Source: UniProtKB-KW
  3. fusion of virus membrane with host plasma membrane Source: InterPro
  4. virion attachment to host cell Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hemagglutinin

Keywords - Biological processi

Clathrin- and caveolin-independent endocytosis of virus by host, Clathrin-mediated endocytosis of virus by host, Fusion of virus membrane with host endosomal membrane, Fusion of virus membrane with host membrane, Host-virus interaction, Viral attachment to host cell, Viral penetration into host cytoplasm, Virus endocytosis by host, Virus entry into host cell

Names & Taxonomyi

Protein namesi
Recommended name:
Hemagglutinin
Cleaved into the following 2 chains:
Gene namesi
Name:HA
OrganismiInfluenza A virus (strain A/Duck/Alberta/35/1976 H1N1)
Taxonomic identifieri352520 [NCBI]
Taxonomic lineageiVirusesssRNA negative-strand virusesOrthomyxoviridaeInfluenzavirus A
Virus hostiAves [TaxID: 8782]
Homo sapiens (Human) [TaxID: 9606]
Sus scrofa (Pig) [TaxID: 9823]

Subcellular locationi

Virion membrane; Single-pass type I membrane protein Reviewed prediction. Host apical cell membrane; Single-pass type I membrane protein
Note: Targeted to the apical plasma membrane in epithelial polarized cells through a signal present in the transmembrane domain. Associated with glycosphingolipid- and cholesterol-enriched detergent-resistant lipid rafts.

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini18 – 529512Extracellular Reviewed prediction
Add
BLAST
Transmembranei530 – 55021Helical; Reviewed prediction
Add
BLAST
Topological domaini551 – 56616Cytoplasmic Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. host cell plasma membrane Source: UniProtKB-SubCell
  2. integral component of membrane Source: UniProtKB-KW
  3. viral envelope Source: UniProtKB-KW
  4. virion membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Host cell membrane, Host membrane, Membrane, Viral envelope protein, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1717 Reviewed prediction
Add
BLAST
Chaini18 – 343326Hemagglutinin HA1 chain
PRO_0000038910Add
BLAST
Chaini345 – 566222Hemagglutinin HA2 chain
PRO_0000038911Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi21 ↔ 481Interchain (between HA1 and HA2 chains) By similarity
Glycosylationi27 – 271N-linked (GlcNAc...); by host Reviewed prediction
Glycosylationi28 – 281N-linked (GlcNAc...); by host Reviewed prediction
Glycosylationi40 – 401N-linked (GlcNAc...); by host Reviewed prediction
Disulfide bondi59 ↔ 292 By similarity
Disulfide bondi72 ↔ 84 By similarity
Glycosylationi104 – 1041N-linked (GlcNAc...); by host Reviewed prediction
Disulfide bondi107 ↔ 153 By similarity
Disulfide bondi296 ↔ 320 By similarity
Glycosylationi304 – 3041N-linked (GlcNAc...); by host Reviewed prediction
Disulfide bondi488 ↔ 492 By similarity
Glycosylationi498 – 4981N-linked (GlcNAc...); by host Reviewed prediction
Lipidationi555 – 5551S-palmitoyl cysteine; by host By similarity
Lipidationi562 – 5621S-palmitoyl cysteine; by host By similarity
Lipidationi565 – 5651S-palmitoyl cysteine; by host By similarity

Post-translational modificationi

In natural infection, inactive HA is matured into HA1 and HA2 outside the cell by one or more trypsin-like, arginine-specific endoprotease secreted by the bronchial epithelial cells. One identified protease that may be involved in this process is secreted in lungs by Clara cells By similarity.
Palmitoylated By similarity.

Keywords - PTMi

Disulfide bond, Glycoprotein, Lipoprotein, Palmitate

Interactioni

Subunit structurei

Homotrimer of disulfide-linked HA1-HA2.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi19 – 257
Beta strandi39 – 446
Beta strandi46 – 483
Beta strandi56 – 616
Helixi74 – 796
Helixi82 – 843
Turni85 – 873
Beta strandi96 – 983
Helixi115 – 1217
Beta strandi123 – 13412
Helixi136 – 1394
Beta strandi150 – 1556
Beta strandi158 – 1603
Beta strandi163 – 1675
Beta strandi170 – 1734
Beta strandi178 – 1836
Beta strandi186 – 19813
Helixi202 – 2098
Beta strandi216 – 2205
Beta strandi223 – 2275
Beta strandi242 – 25110
Beta strandi256 – 2638
Beta strandi265 – 27612
Beta strandi282 – 2843
Beta strandi289 – 2935
Beta strandi295 – 2973
Beta strandi307 – 3104
Beta strandi317 – 3193
Beta strandi330 – 3323
Turni351 – 3533
Beta strandi365 – 3684
Helixi382 – 40120
Helixi419 – 47052
Helixi471 – 4733
Beta strandi474 – 4785
Beta strandi481 – 4866
Helixi490 – 4989

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2WRHX-ray3.00H/J/L18-343[»]
I/K/M345-566[»]
ProteinModelPortaliP26562.
SMRiP26562. Positions 18-519.

Miscellaneous databases

EvolutionaryTraceiP26562.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Family and domain databases

Gene3Di2.10.77.10. 1 hit.
3.90.20.10. 1 hit.
3.90.209.20. 1 hit.
InterProiIPR008980. Capsid_hemagglutn.
IPR013828. Hemagglutn_HA1_a/b_dom.
IPR013827. Hemagglutn_HA1_b-rbn_dom.
IPR000149. Hemagglutn_influenz_A.
IPR001364. Hemagglutn_influenz_A/B.
IPR013829. Hemagglutn_stalk.
[Graphical view]
PfamiPF00509. Hemagglutinin. 1 hit.
[Graphical view]
PRINTSiPR00330. HEMAGGLUTN1.
PR00329. HEMAGGLUTN12.
SUPFAMiSSF49818. SSF49818. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P26562-1 [UniParc]FASTAAdd to Basket

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MEAKLFVLFC TFTVLKADTI CVGYHANNST DTVDTVLEKN VTVTHSVNLL    50
EDSHNGKLCS LNGIAPLQLG KCNVAGWLLG NPECDLLLTA NSWSYIIETS 100
NSENGTCYPG EFIDYEELRE QLSSISSFEK FEIFPKASSW PNHETTKGVT 150
AACSYSGASS FYRNLLWITK KGTSYPKLSK SYTNNKGKEV LVLWGVHHPP 200
SVSEQQSLYQ NADAYVSVGS SKYNRRFAPE IAARPEVRGQ AGRMNYYWTL 250
LDQGDTITFE ATGNLIAPWY AFALNKGSDS GIITSDAPVH NCDTRCQTPH 300
GALNSSLPFQ NVHPITIGEC PKYVKSTKLR MATGLRNVPS IQSRGLFGAI 350
AGFIEGGWTG MIDGWYGYHH QNEQGSGYAA DQKSTQNAID GITSKVNSVI 400
EKMNTQFTAV GKEFNNLERR IENLNKKVDD GFLDVWTYNA ELLVLLENER 450
TLDFHDSNVR NLYEKVKSQL RNNAKEIGNG CFEFYHKCDD ECMESVKNGT 500
YDYPKYSEES KLNREEIDGV KLESMGVYQI LAIYSTVASS LVLLVSWGAI 550
SFWMCSNGSL QCRICI 566
Length:566
Mass (Da):63,072
Last modified:February 1, 1996 - v2
Checksum:i9B7ADCA0E9BCA819
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti65 – 651A → P in AAB52910. 1 Publication
Sequence conflicti125 – 1251I → V in AAB52910. 1 Publication
Sequence conflicti236 – 2361E → K in AAB52910. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D10477 Genomic RNA. Translation: BAA01280.1.
U47310 mRNA. Translation: AAB52910.1.
PIRiA36257. HMIVD1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D10477 Genomic RNA. Translation: BAA01280.1 .
U47310 mRNA. Translation: AAB52910.1 .
PIRi A36257. HMIVD1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2WRH X-ray 3.00 H/J/L 18-343 [» ]
I/K/M 345-566 [» ]
ProteinModelPortali P26562.
SMRi P26562. Positions 18-519.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Miscellaneous databases

EvolutionaryTracei P26562.

Family and domain databases

Gene3Di 2.10.77.10. 1 hit.
3.90.20.10. 1 hit.
3.90.209.20. 1 hit.
InterProi IPR008980. Capsid_hemagglutn.
IPR013828. Hemagglutn_HA1_a/b_dom.
IPR013827. Hemagglutn_HA1_b-rbn_dom.
IPR000149. Hemagglutn_influenz_A.
IPR001364. Hemagglutn_influenz_A/B.
IPR013829. Hemagglutn_stalk.
[Graphical view ]
Pfami PF00509. Hemagglutinin. 1 hit.
[Graphical view ]
PRINTSi PR00330. HEMAGGLUTN1.
PR00329. HEMAGGLUTN12.
SUPFAMi SSF49818. SSF49818. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Molecular analysis of the haemagglutinin gene of an avian H1N1 influenza virus."
    Austin F.J., Kawaoka Y., Webster R.G.
    J. Gen. Virol. 71:2471-2474(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
  2. "Emergence of avian H1N1 influenza viruses in pigs in China."
    Guan Y., Shortridge K.F., Krauss S., Li P.H., Kawaoka Y., Webster R.G.
    J. Virol. 70:8041-8046(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 18-344.

Entry informationi

Entry nameiHEMA_I76A4
AccessioniPrimary (citable) accession number: P26562
Secondary accession number(s): O09652
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: February 1, 1996
Last modified: February 19, 2014
This is version 94 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Miscellaneous

Major glycoprotein, comprises over 80% of the envelope proteins present in virus particle.
The extent of infection into host organism is determined by HA. Influenza viruses bud from the apical surface of polarized epithelial cells (e.g. bronchial epithelial cells) into lumen of lungs and are therefore usually pneumotropic. The reason is that HA is cleaved by tryptase clara which is restricted to lungs. However, HAs of H5 and H7 pantropic avian viruses subtypes can be cleaved by furin and subtilisin-type enzymes, allowing the virus to grow in other organs than lungs.
The influenza A genome consist of 8 RNA segments. Genetic variation of hemagglutinin and/or neuraminidase genes results in the emergence of new influenza strains. The mechanism of variation can be the result of point mutations or the result of genetic reassortment between segments of two different strains.

Keywords - Technical termi

3D-structure

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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