P26525 (NDHI_SYNY3) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NAD(P)H-quinone oxidoreductase subunit I
EC=1.6.5.-

Alternative name(s):
NAD(P)H dehydrogenase I subunit I
NDH-1 subunit I
Short name=NDH-I

Gene names

Name:	ndhI
Ordered Locus Names:	sll0520

Organism

Synechocystis sp. (strain PCC 6803 / Kazusa)

Taxonomic identifier

1111708 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Chroococcales › Synechocystis

Protein attributes

Sequence length	193 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	NDH-1 shuttles electrons from an unknown electron donor, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory and/or the photosynthetic chain. The immediate electron acceptor for the enzyme in this species is believed to be plastoquinone. Couples the redox reaction to proton translocation, and thus conserves the redox energy in a proton gradient By similarity. HAMAP MF_01351
Catalytic activity	NAD(P)H + plastoquinone = NAD(P)+ + plastoquinol. HAMAP MF_01351
Cofactor	Binds 2 4Fe-4S clusters per subunit By similarity. HAMAP MF_01351
Subunit structure	NDH-1 is composed of at least 11 different subunits By similarity.
Subcellular location	Cellular thylakoid membrane; Peripheral membrane protein By similarity HAMAP MF_01351.
Sequence similarities	Belongs to the complex I 23 kDa subunit family. Contains 2 4Fe-4S ferredoxin-type domains.

Ontologies

Keywords
Cellular component	Membrane Thylakoid
Domain	Repeat
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding NAD NADP Plastoquinone
Molecular function	Oxidoreductase
PTM	Quinone
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Cellular component	thylakoid membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW NADH dehydrogenase (ubiquinone) activity Inferred from electronic annotation. Source: InterPro electron carrier activity Inferred from electronic annotation. Source: InterPro metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW quinone binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 193	193	NAD(P)H-quinone oxidoreductase subunit I HAMAP MF_01351		PRO_0000118717
Regions
	Domain	56 – 85	30	4Fe-4S ferredoxin-type 1
	Domain	96 – 125	30	4Fe-4S ferredoxin-type 2
Sites
	Metal binding	65	1	Iron-sulfur 1 (4Fe-4S) By similarity
	Metal binding	68	1	Iron-sulfur 1 (4Fe-4S) By similarity
	Metal binding	71	1	Iron-sulfur 1 (4Fe-4S) By similarity
	Metal binding	75	1	Iron-sulfur 2 (4Fe-4S) By similarity
	Metal binding	105	1	Iron-sulfur 2 (4Fe-4S) By similarity
	Metal binding	108	1	Iron-sulfur 2 (4Fe-4S) By similarity
	Metal binding	111	1	Iron-sulfur 2 (4Fe-4S) By similarity
	Metal binding	115	1	Iron-sulfur 1 (4Fe-4S) By similarity

Sequences

	Sequence		Length	Mass (Da)
	P26525 [UniParc]. Last modified August 1, 1992. Version 1. Checksum: C67389694E391B0E	FASTA	193	22,151
	10 20 30 40 50 60 MFNNILKQVG DYAKESLQAA KYIGQGLAVT FDHMSRRPIT VQYPYEKLIP SERFRGRIHF 70 80 90 100 110 120 EFDKCIACEV CVRVCPINLP VVDWEFNKAV KKKELKHYSI DFGVCIFCGN CVEYCPTNCL 130 140 150 160 170 180 SMTEEYELAA YDRHDLNYDN VALGRLPYKV TEDPMVTPLR ELGYLPKGVI EPHNLPKGSQ 190 RAGQHPEDLV KAE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and transcription analysis of the ndh(A-I-G-E) gene cluster and the ndhD gene of the cyanobacterium Synechocystis sp. PCC6803." Ellersiek U., Steinmueller K. Plant Mol. Biol. 20:1097-1110(1992) [PubMed: 1463844] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. I. Sequence features in the 1 Mb region from map positions 64% to 92% of the genome." Kaneko T., Tanaka A., Sato S., Kotani H., Sazuka T., Miyajima N., Sugiura M., Tabata S. DNA Res. 2:153-166(1995) [PubMed: 8590279] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 27184 / PCC 6803 / N-1.
[3]	"Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions." Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S. , Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S. DNA Res. 3:109-136(1996) [PubMed: 8905231] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 27184 / PCC 6803 / N-1.
[4]	"Immunopurification of a subcomplex of the NAD(P)H-plastoquinone-oxidoreductase from the cyanobacterium Synechocystis sp. PCC6803." Berger S., Ellersiek U., Kinzelt D., Steinmueller K. FEBS Lett. 326:246-250(1993) [PubMed: 8325373] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-25.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X62517 Genomic DNA. Translation: CAA44375.1. BA000022 Genomic DNA. Translation: BAA10884.1.
PIR	FEYBQI. S27973.
RefSeq	NP_442813.1. NC_000911.1.
3D structure databases
ProteinModelPortal	P26525.
ModBase	Search...
Protein-protein interaction databases
IntAct	P26525. 5 interactions.
STRING	P26525.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	952069.
GenomeReviews	Gene locus sll0520 in contig BA000022_GR.
KEGG	syn:sll0520.
NMPDR	fig|1148.1.peg.2914.
PATRIC	23843398. VBISynSp132158_3235.
Phylogenomic databases
eggNOG	COG1143.
HOGENOM	HBG724359.
OMA	HYSIDFG.
PhylomeDB	P26525.
ProtClustDB	PRK05888.
Enzyme and pathway databases
BioCyc	SSP1148:SLL0520-MONOMER.
Family and domain databases
HAMAP	MF_01351. NDH1_NuoI. [Tree]
InterPro	IPR001450. 4Fe4S-bd_dom. IPR017896. 4Fe4S_Fe-S-bd. IPR017900. 4Fe4S_Fe_S_CS. IPR012285. Fum_reductase_C. IPR004497. NADH_plast_OxRdtase_su_I. IPR010226. NADH_quinone_OxRdtase_chainI. [Graphical view]
Gene3D	G3DSA:1.10.1060.10. Fum_reductase_C. 1 hit.
KO	K05580.
Pfam	PF12838. Fer4_7. 1 hit. [Graphical view]
TIGRFAMs	TIGR00403. NdhI. 1 hit. TIGR01971. NuoI. 1 hit.
PROSITE	PS00198. 4FE4S_FER_1. 2 hits. PS51379. 4FE4S_FER_2. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NDHI_SYNY3

Accession

Primary (citable) accession number: P26525

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1992
Last sequence update:	August 1, 1992
Last modified:	January 25, 2012
This is version 98 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program