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Reviewed, UniProtKB/Swiss-Prot P26518 (G3PC_MAGLI)

Last modified June 16, 2009. Version 55. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Glyceraldehyde-3-phosphate dehydrogenase, cytosolic
    EC=1.2.1.12
Gene names
Name: GAPC
Synonyms: GAPDH
OrganismMagnolia liliiflora
Taxonomic identifier3403 [NCBI]
Taxonomic lineageEukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › magnoliids › Magnoliales › Magnoliaceae › Magnolia

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Protein attributes

Sequence length341 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at transcript level.

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General annotation (Comments)

Catalytic activity

D-glyceraldehyde 3-phosphate + phosphate + NAD+ = 3-phospho-D-glyceroyl phosphate + NADH.

Pathway

Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5.

Subunit structure

Homotetramer.

Subcellular location

Cytoplasm.

Miscellaneous

Plants contain three forms of GAPDH: a cytosolic form which participates in glycolysis and two chloroplast forms which participates in photosynthesis. These three forms are encoded by distinct genes.

Sequence similarities

Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.

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Ontologies

Keywords
   Biological processGlycolysis
   Cellular componentCytoplasm
   LigandNAD
   Molecular functionOxidoreductase
Gene Ontology (GO)
   Biological processglycolysis

Inferred from electronic annotation. Source: UniProtKB-KW

oxidation reduction

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionNAD or NADH binding

Inferred from electronic annotation. Source: InterPro

glyceraldehyde-3-phosphate dehydrogenase (phosphorylating) activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 341341Glyceraldehyde-3-phosphate dehydrogenase, cytosolic
PRO_0000145604

Regions

Nucleotide binding15 – 162NAD By similarity
Region155 – 1573Glyceraldehyde 3-phosphate binding By similarity
Region215 – 2162Glyceraldehyde 3-phosphate binding By similarity

Sites

Active site1561Nucleophile By similarity
Binding site371NAD By similarity
Binding site841NAD; via carbonyl oxygen By similarity
Binding site1861Glyceraldehyde 3-phosphate By similarity
Binding site2381Glyceraldehyde 3-phosphate By similarity
Binding site3201NAD By similarity
Site1831Activates thiol group during catalysis By similarity

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Sequences

Sequence LengthMass (Da)Tools
P26518-1 [UniParc].

Last modified August 1, 1992. Version 1.
Checksum: DA1B98CDB9BE2F51

FASTA34136,982
        10         20         30         40         50         60 
MGGKKIKIGI NGFGRIGRLV ARVALQRDDV ELVAVNDPFI TTDYMTYMFK YDSVHGQWKH 

        70         80         90        100        110        120 
HELKVKDSKT LLFGEKPVTV FGVRNPEEIP WGETGAEFVV ESTGVFTDKD KAAAHLKGGA 

       130        140        150        160        170        180 
KKVIISAPSK DAPMFVVGVN EHEYKSNIDI VSNASCTTNC LAPLAKVIND KFGIVEGLMT 

       190        200        210        220        230        240 
TVHSITATQK TVDGPSSKDW RGGRAAGFNI IPSSTGAAKA VGKVLPALNG KLTGMAFRVP 

       250        260        270        280        290        300 
TVDVSVVDLT VRLEKAASYE EIKAVIKAES EGKLKGILGY TEEDVVSTDF IGDNRSSIFD 

       310        320        330        340 
AKAGIALNEH FVKLVSWYDN EWGYSSRVID LILIVHMASC Q

« Hide

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References

[1]"Molecular evidence for pre-Cretaceous angiosperm origins."
Martin W., Gierl A., Saedler H.
Nature 339:46-48(1989)
Cited for: NUCLEOTIDE SEQUENCE [MRNA].

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Cross-references

Sequence databases

X60347 mRNA. Translation: CAA42905.1.
PIRDEJMG. S18483.

3D structure databases

HSSPHSSP built from PDB template 1DSS based on UniProtKB P56649.
ModBaseSearch...

Family and domain databases

InterProIPR000173. GlycerAld_3-P_DH.
IPR006424. Glyceraldehyde-3-P_DH_1.
[Graphical view]
PANTHERPTHR10836. GAP_DH. 1 hit.
PfamPF02800. Gp_dh_C. 1 hit.
PF00044. Gp_dh_N. 1 hit.
[Graphical view]
PIRSFPIRSF000149. GAP_DH. 1 hit.
PRINTSPR00078. G3PDHDRGNASE.
TIGRFAMsTIGR01534. GAPDH-I. 1 hit.
PROSITEPS00071. GAPDH. 1 hit.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry nameG3PC_MAGLI
AccessionPrimary (citable) accession number: P26518
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: August 1, 1992
Last modified: June 16, 2009
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectPPAP (Plant Proteome Annotation Project)

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Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents