Reviewed, UniProtKB/Swiss-Prot P26512 (AK_CORGL)

Names and origin

Protein names

Recommended name:
    Aspartokinase
    EC=2.7.2.4
Alternative name(s):
    Aspartate kinase

Gene names

Name:	lysC
Ordered Locus Names:	Cgl0251, cg0306

Organism

Corynebacterium glutamicum (Brevibacterium flavum) [Complete proteome] [HAMAP]

Taxonomic identifier

1718 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Corynebacteriaceae › Corynebacterium

Protein attributes

Sequence length	421 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	ATP + L-aspartate = ADP + 4-phospho-L-aspartate.
Enzyme regulation	Feedback inhibition by lysine and threonine.
Pathway	Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; tetrahydrodipicolinate from L-aspartate: step 1/4. Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-homoserine from L-aspartate: step 1/3. Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 1/5.
Subunit structure	Tetramer consisting of two isoforms Alpha (catalytic) and two isoforms Beta (function not known).
Sequence similarities	Belongs to the aspartokinase family. Contains 2 ACT domains.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Diaminopimelate biosynthesis Lysine biosynthesis
Coding sequence diversity	Alternative initiation
Domain	Repeat
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	diaminopimelate biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW amino acid binding Inferred from electronic annotation. Source: InterPro aspartate kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative initiation. [Align] [Select]

Isoform Alpha (identifier: P26512-1)

Also known as: Aspartokinase subunit alpha;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform Beta (identifier: P26512-2)

Also known as: Aspartokinase subunit beta;

The sequence of this isoform differs from the canonical sequence as follows:
     1-249: Missing.
     250-250: V → M

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 421

421

Aspartokinase

PRO_0000002379

Regions

Domain

266 – 339

74

ACT 1

Domain

348 – 413

66

ACT 2

Natural variations

Alternative sequence

1 – 249

249

Missing in isoform Beta.

VSP_018659

Alternative sequence

250

1

V → M in isoform Beta.

VSP_018660

Experimental info

Mutagenesis

301

1

S → Y: Feedback-resistant and enhanced expression of the asd gene.

Sequence conflict

40

1

C → V Ref.1

Sequence conflict

40

1

C → V Ref.4

Secondary structure

1

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421

Helix Strand Turn

Details...

Beta strand

254 – 261

8

Beta strand

263 – 273

11

Helix

278 – 288

11

Beta strand

295 – 298

4

Turn

303 – 305

3

Beta strand

307 – 315

9

Helix

316 – 318

3

Helix

319 – 327

9

Turn

328 – 334

7

Beta strand

336 – 342

7

Beta strand

344 – 352

9

Helix

358 – 370

13

Beta strand

377 – 381

5

Beta strand

384 – 390

7

Helix

391 – 393

3

Helix

394 – 405

12

Sequences

	Sequence		Length	Mass (Da)
	Isoform Alpha (Aspartokinase subunit alpha) [UniParc]. Last modified July 11, 2002. Version 2. Checksum: E36B4D0081DE0827	FASTA	421	44,755
	10 20 30 40 50 60 MALVVQKYGG SSLESAERIR NVAERIVATK KAGNDVVVVC SAMGDTTDEL LELAAAVNPV 70 80 90 100 110 120 PPAREMDMLL TAGERISNAL VAMAIESLGA EAQSFTGSQA GVLTTERHGN ARIVDVTPGR 130 140 150 160 170 180 VREALDEGKI CIVAGFQGVN KETRDVTTLG RGGSDTTAVA LAAALNADVC EIYSDVDGVY 190 200 210 220 230 240 TADPRIVPNA QKLEKLSFEE MLELAAVGSK ILVLRSVEYA RAFNVPLRVR SSYSNDPGTL 250 260 270 280 290 300 IAGSMEDIPV EEAVLTGVAT DKSEAKVTVL GISDKPGEAA KVFRALADAE INIDMVLQNV 310 320 330 340 350 360 SSVEDGTTDI TFTCPRSDGR RAMEILKKLQ VQGNWTNVLY DDQVGKVSLV GAGMKSHPGV 370 380 390 400 410 420 TAEFMEALRD VNVNIELIST SEIRISVLIR EDDLDAAARA LHEQFQLGGE DEAVVYAGTG R
	Isoform Beta (Aspartokinase subunit beta). Checksum: 140CBE126C299ED6	FASTA	172	18,537
	10 20 30 40 50 60 MEEAVLTGVA TDKSEAKVTV LGISDKPGEA AKVFRALADA EINIDMVLQN VSSVEDGTTD 70 80 90 100 110 120 ITFTCPRSDG RRAMEILKKL QVQGNWTNVL YDDQVGKVSL VGAGMKSHPG VTAEFMEALR 130 140 150 160 170 DVNVNIELIS TSEIRISVLI REDDLDAAAR ALHEQFQLGG EDEAVVYAGT GR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Genetic and biochemical analysis of the aspartokinase from Corynebacterium glutamicum." Kalinowski J., Cremer J., Bachmann B., Eggeling L., Sahm H., Puehler A. Mol. Microbiol. 5:1197-1204(1991) [PubMed: 1956296] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.
[2]	"Complete genomic sequence of Corynebacterium glutamicum ATCC 13032." Nakagawa S. Submitted (MAY-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.
[3]	"The complete Corynebacterium glutamicum ATCC 13032 genome sequence and its impact on the production of L-aspartate-derived amino acids and vitamins." Kalinowski J., Bathe B., Bartels D., Bischoff N., Bott M., Burkovski A., Dusch N., Eggeling L., Eikmanns B.J., Gaigalat L., Goesmann A., Hartmann M., Huthmacher K., Kraemer R., Linke B., McHardy A.C., Meyer F., Moeckel B. , Pfefferle W., Puehler A., Rey D.A., Rueckert C., Rupp O., Sahm H., Wendisch V.F., Wiegraebe I., Tauch A. J. Biotechnol. 104:5-25(2003) [PubMed: 12948626] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.
[4]	"Leucine synthesis in Corynebacterium glutamicum: enzyme activities, structure of leuA, and effect of leuA inactivation on lysine synthesis." Patek M., Krumbach K., Eggeling L., Sahm H. Appl. Environ. Microbiol. 60:133-140(1994) [PubMed: 8117072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-51. Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.
[5]	"Aspartokinase genes lysC alpha and lysC beta overlap and are adjacent to the aspartate beta-semialdehyde dehydrogenase gene asd in Corynebacterium glutamicum." Kalinowski J., Bachmann B., Thierbach G., Puehler A. Mol. Gen. Genet. 224:317-324(1990) [PubMed: 1980002] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 158-421. Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

X57226 Genomic DNA. Translation: CAA40502.1.
X57226 Genomic DNA. Translation: CAA40503.1.
BA000036 Genomic DNA. Translation: BAB97644.1.
BX927148 Genomic DNA. Translation: CAF18822.1.
X70959 Genomic DNA. Translation: CAA50296.1. Sequence problems.

PIR

I40723.
S15276.

RefSeq

NP_599504.1.
YP_224551.1.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2DTJ	X-ray	1.58	A/B	251-421	[»]

ModBase

Search...

Genome annotation databases

GeneID

1021294.
3345161.

GenomeReviews

Gene locus Cgl0251 in contig BA000036_GR.
Gene locus cg0306 in contig BX927147_GR.

KEGG

cgb:cg0306.
cgl:NCgl0247.

Organism-specific databases

CMR

Search...

Phylogenomic databases

HOGENOM

P26512.

OMA

P26512. YEEMLEM.

Enzyme and pathway databases

BioCyc

CGLU196627-1:CG0306-MON.
MetaCyc:MON-6461.
MetaCyc:MON-6462.

BRENDA

2.7.2.4. 812.

Family and domain databases

InterPro

IPR002912. ACT_bd.
IPR001048. Asp/Glu/Uridylate_kinase.
IPR005260. Asp_kin_monofn.
IPR001341. Asp_kin_reg.
IPR018042. Aspartate_kinase_CS.
[Graphical view]

Gene3D

G3DSA:3.40.1160.10. Aa_kinase. 1 hit.

Pfam

PF00696. AA_kinase. 1 hit.
PF01842. ACT. 2 hits.
[Graphical view]

TIGRFAMs

TIGR00656. asp_kin_monofn. 1 hit.
TIGR00657. asp_kinases. 1 hit.

PROSITE

PS00324. ASPARTOKINASE. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

AK_CORGL

Accession

Primary (citable) accession number: P26512
Secondary accession number(s): Q59286

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1992
Last sequence update:	July 11, 2002
Last modified:	June 16, 2009
This is version 83 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)