Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

5-aminolevulinate synthase

Gene

hemA

Organism
Rhizobium radiobacter (Agrobacterium tumefaciens) (Agrobacterium radiobacter)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

Succinyl-CoA + glycine = 5-aminolevulinate + CoA + CO2.

Cofactori

pyridoxal 5'-phosphateBy similarity

Pathwayi: protoporphyrin-IX biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes 5-aminolevulinate from glycine.
Proteins known to be involved in this subpathway in this organism are:
  1. 5-aminolevulinate synthase (hemA), 5-aminolevulinate synthase (A6U94_11960), 5-aminolevulinate synthase (A6U96_12335), 5-aminolevulinate synthase (A7J57_19820), 5-aminolevulinate synthase (AWN88_17950), 5-aminolevulinate synthase (SY94_2444), 5-aminolevulinate synthase (hemA), 5-aminolevulinate synthase (BN949_02205), 5-aminolevulinate synthase (RU07_23400), 5-aminolevulinate synthase (A6U90_11690)
This subpathway is part of the pathway protoporphyrin-IX biosynthesis, which is itself part of Porphyrin-containing compound metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 5-aminolevulinate from glycine, the pathway protoporphyrin-IX biosynthesis and in Porphyrin-containing compound metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei21SubstrateBy similarity1
Binding sitei137SubstrateBy similarity1
Binding sitei189Pyridoxal phosphateBy similarity1
Binding sitei217Pyridoxal phosphateBy similarity1
Binding sitei245Pyridoxal phosphateBy similarity1
Active sitei248By similarity1
Binding sitei277Pyridoxal phosphate; shared with dimeric partnerBy similarity1
Binding sitei278Pyridoxal phosphate; shared with dimeric partnerBy similarity1
Binding sitei363SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Keywords - Biological processi

Heme biosynthesis

Keywords - Ligandi

Pyridoxal phosphate

Enzyme and pathway databases

UniPathwayiUPA00251; UER00375.

Names & Taxonomyi

Protein namesi
Recommended name:
5-aminolevulinate synthase (EC:2.3.1.37)
Alternative name(s):
5-aminolevulinic acid synthase
Delta-ALA synthase
Delta-aminolevulinate synthase
Gene namesi
Name:hemA
OrganismiRhizobium radiobacter (Agrobacterium tumefaciens) (Agrobacterium radiobacter)
Taxonomic identifieri358 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeRhizobium/Agrobacterium groupAgrobacteriumAgrobacterium tumefaciens complex

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001638241 – 4055-aminolevulinate synthaseAdd BLAST405

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei248N6-(pyridoxal phosphate)lysineBy similarity1

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi1050720.Agau_C100637.

Structurei

3D structure databases

ProteinModelPortaliP26505.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4107EEK. Bacteria.
COG0156. LUCA.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
InterProiIPR010961. 4pyrrol_synth_NH2levulA_synth.
IPR001917. Aminotrans_II_pyridoxalP_BS.
IPR004839. Aminotransferase_I/II.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PfamiPF00155. Aminotran_1_2. 1 hit.
[Graphical view]
SUPFAMiSSF53383. SSF53383. 1 hit.
TIGRFAMsiTIGR01821. 5aminolev_synth. 1 hit.
PROSITEiPS00599. AA_TRANSFER_CLASS_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P26505-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDFEAFFTTE LQSLHSEGRY RVFADIERQQ GNFPRATRYN ANGQRKDVTV
60 70 80 90 100
WCSNDYLGMG QNPKVIEAMK AAIDHCGAGA GGTRNISGTN HYHVLLEQEL
110 120 130 140 150
ADLHGKESAL IFTSGYVSNW ATLGTLGQKI PGLIIFSDAL NHASMIEGIR
160 170 180 190 200
YGRCERVIWK HNDLEDLEAK LKAADPNAPK LIAFESVYSM DGDIAPIKEI
210 220 230 240 250
CDLADRYGAM TYLDEVHAVG MYGPRGGGIA EREGLMDRLT IIEGTLGKAF
260 270 280 290 300
GVMGGYITGS TAVCDFIRSF ASGFIFTTAL PPSLAAGAIA SIQHLKASPF
310 320 330 340 350
ERARHQDRVR KLRGLLDARG IPHMDNPSHI VPVMVGDAAK CKWISDILLD
360 370 380 390 400
SHGVYVQPIN YPTVPRKTER LRITPTPLHS DADIEHLVGA LHQLWSHCAL

ARAVA
Length:405
Mass (Da):44,412
Last modified:August 1, 1992 - v1
Checksum:iF173AC1B13CAACDA
GO

Sequence databases

PIRiS15996.

Cross-referencesi

Sequence databases

PIRiS15996.

3D structure databases

ProteinModelPortaliP26505.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi1050720.Agau_C100637.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

eggNOGiENOG4107EEK. Bacteria.
COG0156. LUCA.

Enzyme and pathway databases

UniPathwayiUPA00251; UER00375.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
InterProiIPR010961. 4pyrrol_synth_NH2levulA_synth.
IPR001917. Aminotrans_II_pyridoxalP_BS.
IPR004839. Aminotransferase_I/II.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PfamiPF00155. Aminotran_1_2. 1 hit.
[Graphical view]
SUPFAMiSSF53383. SSF53383. 1 hit.
TIGRFAMsiTIGR01821. 5aminolev_synth. 1 hit.
PROSITEiPS00599. AA_TRANSFER_CLASS_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiHEM1_RHIRD
AccessioniPrimary (citable) accession number: P26505
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: August 1, 1992
Last modified: October 5, 2016
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.