P26505 (HEM1_RHIRD) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
5-aminolevulinate synthase
EC=2.3.1.37

Alternative name(s):
5-aminolevulinic acid synthase
Delta-ALA synthase
Delta-aminolevulinate synthase

Gene names

Name:

hemA

Organism

Rhizobium radiobacter (Agrobacterium tumefaciens) (Agrobacterium radiobacter)

Taxonomic identifier

358 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Rhizobium/Agrobacterium group › Agrobacterium

Protein attributes

Sequence length	405 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Succinyl-CoA + glycine = 5-aminolevulinate + CoA + CO2.
Cofactor	Pyridoxal phosphate.
Pathway	Porphyrin metabolism; protoporphyrin-IX biosynthesis; 5-aminolevulinate from glycine: step 1/1.
Sequence similarities	Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family.

Ontologies

Keywords
Biological process	Heme biosynthesis
Ligand	Pyridoxal phosphate
Molecular function	Acyltransferase Transferase
Gene Ontology (GO)
Biological process	heme biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	5-aminolevulinate synthase activity Inferred from electronic annotation. Source: EC pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro transferase activity, transferring nitrogenous groups Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 405	405	5-aminolevulinate synthase		PRO_0000163824
Amino acid modifications
	Modified residue	248	1	N6-(pyridoxal phosphate)lysine Probable

Sequences

	Sequence		Length	Mass (Da)
	P26505 [UniParc]. Last modified August 1, 1992. Version 1. Checksum: F173AC1B13CAACDA	FASTA	405	44,412
	10 20 30 40 50 60 MDFEAFFTTE LQSLHSEGRY RVFADIERQQ GNFPRATRYN ANGQRKDVTV WCSNDYLGMG 70 80 90 100 110 120 QNPKVIEAMK AAIDHCGAGA GGTRNISGTN HYHVLLEQEL ADLHGKESAL IFTSGYVSNW 130 140 150 160 170 180 ATLGTLGQKI PGLIIFSDAL NHASMIEGIR YGRCERVIWK HNDLEDLEAK LKAADPNAPK 190 200 210 220 230 240 LIAFESVYSM DGDIAPIKEI CDLADRYGAM TYLDEVHAVG MYGPRGGGIA EREGLMDRLT 250 260 270 280 290 300 IIEGTLGKAF GVMGGYITGS TAVCDFIRSF ASGFIFTTAL PPSLAAGAIA SIQHLKASPF 310 320 330 340 350 360 ERARHQDRVR KLRGLLDARG IPHMDNPSHI VPVMVGDAAK CKWISDILLD SHGVYVQPIN 370 380 390 400 YPTVPRKTER LRITPTPLHS DADIEHLVGA LHQLWSHCAL ARAVA

References

[1]	"Cloning and nucleotide sequence of the hemA gene of Agrobacterium radiobacter." Drolet M., Sasarman A. Mol. Gen. Genet. 226:250-256(1991) [PubMed: 2034217] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 4718 / JCM 20199 / IAM 1526 / LMG 139 / NBRC 12664 / CIP 104326.
+	Additional computationally mapped references.

Cross-references

Sequence databases
PIR	S15996.
3D structure databases
ProteinModelPortal	P26505.
SMR	P26505. Positions 2-395.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR010961. 4pyrrol_synth_NH2levulA_synth. IPR001917. Aminotrans_II_pyridoxalP_BS. IPR004839. Aminotransferase_I/II. IPR015424. PyrdxlP-dep_Trfase_major_dom. IPR015421. PyrdxlP-dep_Trfase_major_sub1. IPR015422. PyrdxlP-dep_Trfase_major_sub2. [Graphical view]
Gene3D	G3DSA:3.40.640.10. PyrdxlP-dep_Trfase_major_sub1. 1 hit. G3DSA:3.90.1150.10. PyrdxlP-dep_Trfase_major_sub2. 1 hit.
Pfam	PF00155. Aminotran_1_2. 1 hit. [Graphical view]
SUPFAM	SSF53383. PyrdxlP-dep_Trfase_major. 1 hit.
TIGRFAMs	TIGR01821. 5aminolev_synth. 1 hit.
PROSITE	PS00599. AA_TRANSFER_CLASS_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HEM1_RHIRD

Accession

Primary (citable) accession number: P26505

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1992
Last sequence update:	August 1, 1992
Last modified:	May 31, 2011
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program