Reviewed, UniProtKB/Swiss-Prot P26504 (LIPA_PSEFL)

Names and origin

Protein names	Recommended name:     Lipase     EC=3.1.1.3 Alternative name(s):     Triacylglycerol lipase
Organism	Pseudomonas fluorescens
Taxonomic identifier	294 [NCBI]
Taxonomic lineage	Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	449 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	Triacylglycerol + H2O = diacylglycerol + a carboxylate.
Sequence similarities	Belongs to the AB hydrolase superfamily. Lipase family. Contains 2 hemolysin-type calcium-binding repeats.
Biophysicochemical properties	Temperature dependence: Thermostable.

Ontologies

Keywords
Biological process	Lipid degradation
Domain	Repeat Signal
Molecular function	Hydrolase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	lipid catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	calcium ion binding Inferred from electronic annotation. Source: InterPro triglyceride lipase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 23	23	Potential
	Chain	24 – 449	426	Lipase		PRO_0000017742
Regions
	Repeat	372 – 389	18	Hemolysin-type calcium-binding 1
	Repeat	390 – 407	18	Hemolysin-type calcium-binding 2
Sites
	Active site	206	1	Charge relay system By similarity

Sequences

	Sequence		Length	Mass (Da)
	P26504-1 [UniParc]. Last modified August 1, 1992. Version 1. Checksum: 16E539323D5D0DD8	FASTA	449	48,233
	10 20 30 40 50 60 MGVFDYKNLG TEASKTLFAD ATAITLYTYH NLDNGFAVGY QQHGLGLGCR HTGRGVARQH 70 80 90 100 110 120 RLPGSDPPAF PGILTRKRPP WTRCTQPVGR QSSASALGYG GKVDARGTFF GEKAGYTTAQ 130 140 150 160 170 180 AEVLGKYDDA GKLLEIGIGF RGTSGPRESL ITTPCRSGQR PARRAGPQGL CEKLCRRTFG 190 200 210 220 230 240 GLLKTVADYA GAHGLSGKDV LVSGHSLGGL AVNSMADLST SKWAGFYKDA NYLAYASPTQ 250 260 270 280 290 300 SAGDKVLNIG YENDPVFRAL DGSTFNLSSL GVHDKAHEST TDNIVSFNDH YASTLWNVLP 310 320 330 340 350 360 FSIANLSTWV SHLPSAYGDG MTRVLESGFY EQMTRDSTII LCPTWSDPAR ANTWVQDLNR 370 380 390 400 410 420 NAEPHTGNTF IIGSDGNDLI QGGKGADFIE GGKGNDTIRD NSGHNTFLFS GHFGQDRIIG 430 440 YQPTGWCSRA PTAAPTCATT RRPWGPIRC

References

[1]

"Cloning and nucleotide sequence of thermostable lipase gene from Pseudomonas fluorescens SIK W1." Chung G.H., Lee Y.P., Jeohn G.H., Yoo O.J., Rhee J.S. Agric. Biol. Chem. 55:2359-2365(1991) [PubMed: 1368740] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE. Strain: SIK W1.

Cross-references

Sequence databases
EMBLGenBankDDBJ	S77830 Genomic DNA. Translation: AAC60402.1. D11455 Genomic DNA. Translation: BAA02012.1.
PIR	JQ1277.
3D structure databases
ModBase	Search...
Enzyme and pathway databases
BRENDA	3.1.1.3. 329.
Family and domain databases
InterPro	IPR001343. Hemolysn_Ca-bd. IPR018512. Hemolysn_Ca-bd_NodA. IPR018511. Hemolysn_Ca-bd_sg. IPR002921. Lipase_3. IPR008262. Lipase_Ser_AS. [Graphical view]
Pfam	PF00353. HemolysinCabind. 2 hits. PF01764. Lipase_3. 1 hit. [Graphical view]
PRINTS	PR00313. CABNDNGRPT.
PROSITE	PS00330. HEMOLYSIN_CALCIUM. 1 hit. PS00120. LIPASE_SER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LIPA_PSEFL

Accession

Primary (citable) accession number: P26504

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1992
Last sequence update:	August 1, 1992
Last modified:	September 22, 2009
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)