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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Methanothermobacter marburgensis (strain DSM 2133 / 14651 / NBRC 100331 / OCM 82 / Marburg) (Methanobacterium thermoautotrophicum)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei594 – 5941ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciMMAR79929:GH5J-1768-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:MTBMA_c17620
OrganismiMethanothermobacter marburgensis (strain DSM 2133 / 14651 / NBRC 100331 / OCM 82 / Marburg) (Methanobacterium thermoautotrophicum)
Taxonomic identifieri79929 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanobacteriaMethanobacterialesMethanobacteriaceaeMethanothermobacter
ProteomesiUP000000345 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi589 – 5891G → D: Resistance to mupirocin (pseudomonic acid A).

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed
Chaini2 – 10451044Isoleucine--tRNA ligasePRO_0000098579Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliP26499.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi49 – 5911"HIGH" regionAdd
BLAST
Motifi591 – 5955"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000246403.
KOiK01870.
OMAiPCECGGT.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02003. Ile_tRNA_synth_type2.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR023586. Ile-tRNA-ligase_type2.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P26499-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPIQEAEKSY KPHVIEEKVQ SFWEERDIYE RVKELREEGP RYSFLDGPPY
60 70 80 90 100
CSGRIHLGTA WNKIMKDSYL RFKSMRGFNV RRQPGWDTHG LPIEHKVEGI
110 120 130 140 150
LGVRSKKDIE DKIGIEEFVR KCREFAMENK AVMTSQFQRL GVWMDWDDPY
160 170 180 190 200
VTFDPAYMES CWWTLKRAHE KDLLLRDLRV ITWCPRCETA LALAEIDYHE
210 220 230 240 250
KEDPSIYVKF PVSGDTYILV WTTTPWTLPA NMAVAVHPDF DYAHTRLDGE
260 270 280 290 300
TYIMAEALVE KVLGEEAEII KTVRGSELEG LTYRHPLDEE VPCHRDMEHR
310 320 330 340 350
VILGDHVTLT EGTGCVHTAP GHGPEDFEIG KEYGLPVFCP VDEAGVFTED
360 370 380 390 400
AGKYRGLFVK DADSDIIDDL RSKNLLLRAE TISHRYGFCW RCKTPIIYLA
410 420 430 440 450
TEQWFLKITE IKDKMLSELD RVQWIPSWAG ESRFRNWIEN ARDWTISRQR
460 470 480 490 500
YWGIPIPIWV CEDCDSIHVV GSIGELRELA VEGQLEGDFI HRPHVDRIIL
510 520 530 540 550
ECGRCGGRMK RTPDVLDVWI DSGVAGWAAL HYPREKELFS EWFPYDFITE
560 570 580 590 600
GHDQTRGWFY SQLGCGVIAL DETPYRRVLM HGFTLDEEGR KMSKSLGNVV
610 620 630 640 650
EPEDVIEKYG ADVLRFYLLW ANKPWEDLKF VWDELKNVNK MFNILWNVYV
660 670 680 690 700
FATTYMSLDR FQPGDHELED LHFRDEDRWI ISRVNSVALK VTEALDNLHF
710 720 730 740 750
HRATREIHDF IVEDLSRWYI RLIRSRTWIE RDDPDKLAAY HSLYTALKTL
760 770 780 790 800
IVTLSPIAPH VCEDIYQNLV RGAEPDSPES IHMLDWMVSE DAVDGKLEAE
810 820 830 840 850
MDIVREIIEA CARARDTARY KLRWPVREIV VVSEDDKVLE AAESLKGVIA
860 870 880 890 900
EQANAKSIRT STEFPDMRII AKPNPATLGP KLRQDMPPVM RKLEEADGAE
910 920 930 940 950
VKAALESEGS FRVDLDGRII VLEPDDIIFE TELPENIVNA QFEGGSVFVD
960 970 980 990 1000
TELTPEIMSE AMARELVRRI QDMRKDLDLD VEARIEVSVK CSPEFRELTE
1010 1020 1030 1040
PQREFVENEV RASHLSFDYT ELEYTKEWKI SDENLIISIK PSDKV
Length:1,045
Mass (Da):121,362
Last modified:January 23, 2007 - v3
Checksum:i5A935B0FE300077E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M59245 Genomic DNA. Translation: AAA72950.1.
CP001710 Genomic DNA. Translation: ADL59330.1.
RefSeqiWP_013296540.1. NC_014408.1.
YP_003850643.1. NC_014408.1.

Genome annotation databases

EnsemblBacteriaiADL59330; ADL59330; MTBMA_c17620.
GeneIDi9705473.
KEGGimmg:MTBMA_c17620.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M59245 Genomic DNA. Translation: AAA72950.1.
CP001710 Genomic DNA. Translation: ADL59330.1.
RefSeqiWP_013296540.1. NC_014408.1.
YP_003850643.1. NC_014408.1.

3D structure databases

ProteinModelPortaliP26499.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiADL59330; ADL59330; MTBMA_c17620.
GeneIDi9705473.
KEGGimmg:MTBMA_c17620.

Phylogenomic databases

HOGENOMiHOG000246403.
KOiK01870.
OMAiPCECGGT.

Enzyme and pathway databases

BioCyciMMAR79929:GH5J-1768-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02003. Ile_tRNA_synth_type2.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR023586. Ile-tRNA-ligase_type2.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Isoleucyl-tRNA synthetase of Methanobacterium thermoautotrophicum Marburg. Cloning of the gene, nucleotide sequence, and localization of a base change conferring resistance to pseudomonic acid."
    Jenal U., Rechsteiner T., Tan P.-Y., Buehlmann E., Meile L., Leisinger T.
    J. Biol. Chem. 266:10570-10577(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE.
    Strain: DSM 2133 / 14651 / NBRC 100331 / OCM 82 / Marburg.
  2. "Complete genome sequence of Methanothermobacter marburgensis, a methanoarchaeon model organism."
    Liesegang H., Kaster A.K., Wiezer A., Goenrich M., Wollherr A., Seedorf H., Gottschalk G., Thauer R.K.
    J. Bacteriol. 192:5850-5851(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DSM 2133 / 14651 / NBRC 100331 / OCM 82 / Marburg.

Entry informationi

Entry nameiSYI_METTM
AccessioniPrimary (citable) accession number: P26499
Secondary accession number(s): D9PYN2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: January 23, 2007
Last modified: May 27, 2015
This is version 99 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.