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P26499

- SYI_METTM

UniProt

P26499 - SYI_METTM

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Protein

Isoleucine--tRNA ligase

Gene
ileS, MTBMA_c17620
Organism
Methanothermobacter marburgensis (strain DSM 2133 / 14651 / NBRC 100331 / OCM 82 / Marburg) (Methanobacterium thermoautotrophicum)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zinc By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei594 – 5941ATP By similarity

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciMMAR79929:GH5J-1768-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:MTBMA_c17620
OrganismiMethanothermobacter marburgensis (strain DSM 2133 / 14651 / NBRC 100331 / OCM 82 / Marburg) (Methanobacterium thermoautotrophicum)
Taxonomic identifieri79929 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanobacteriaMethanobacterialesMethanobacteriaceaeMethanothermobacter
ProteomesiUP000000345: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi589 – 5891G → D: Resistance to mupirocin (pseudomonic acid A).

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedUniRule annotation
Chaini2 – 10451044Isoleucine--tRNA ligaseUniRule annotationPRO_0000098579Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliP26499.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi49 – 5911"HIGH" regionUniRule annotationAdd
BLAST
Motifi591 – 5955"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

HOGENOMiHOG000246403.
KOiK01870.
OMAiKPVHWCL.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02003. Ile_tRNA_synth_type2.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P26499-1 [UniParc]FASTAAdd to Basket

« Hide

MPIQEAEKSY KPHVIEEKVQ SFWEERDIYE RVKELREEGP RYSFLDGPPY     50
CSGRIHLGTA WNKIMKDSYL RFKSMRGFNV RRQPGWDTHG LPIEHKVEGI 100
LGVRSKKDIE DKIGIEEFVR KCREFAMENK AVMTSQFQRL GVWMDWDDPY 150
VTFDPAYMES CWWTLKRAHE KDLLLRDLRV ITWCPRCETA LALAEIDYHE 200
KEDPSIYVKF PVSGDTYILV WTTTPWTLPA NMAVAVHPDF DYAHTRLDGE 250
TYIMAEALVE KVLGEEAEII KTVRGSELEG LTYRHPLDEE VPCHRDMEHR 300
VILGDHVTLT EGTGCVHTAP GHGPEDFEIG KEYGLPVFCP VDEAGVFTED 350
AGKYRGLFVK DADSDIIDDL RSKNLLLRAE TISHRYGFCW RCKTPIIYLA 400
TEQWFLKITE IKDKMLSELD RVQWIPSWAG ESRFRNWIEN ARDWTISRQR 450
YWGIPIPIWV CEDCDSIHVV GSIGELRELA VEGQLEGDFI HRPHVDRIIL 500
ECGRCGGRMK RTPDVLDVWI DSGVAGWAAL HYPREKELFS EWFPYDFITE 550
GHDQTRGWFY SQLGCGVIAL DETPYRRVLM HGFTLDEEGR KMSKSLGNVV 600
EPEDVIEKYG ADVLRFYLLW ANKPWEDLKF VWDELKNVNK MFNILWNVYV 650
FATTYMSLDR FQPGDHELED LHFRDEDRWI ISRVNSVALK VTEALDNLHF 700
HRATREIHDF IVEDLSRWYI RLIRSRTWIE RDDPDKLAAY HSLYTALKTL 750
IVTLSPIAPH VCEDIYQNLV RGAEPDSPES IHMLDWMVSE DAVDGKLEAE 800
MDIVREIIEA CARARDTARY KLRWPVREIV VVSEDDKVLE AAESLKGVIA 850
EQANAKSIRT STEFPDMRII AKPNPATLGP KLRQDMPPVM RKLEEADGAE 900
VKAALESEGS FRVDLDGRII VLEPDDIIFE TELPENIVNA QFEGGSVFVD 950
TELTPEIMSE AMARELVRRI QDMRKDLDLD VEARIEVSVK CSPEFRELTE 1000
PQREFVENEV RASHLSFDYT ELEYTKEWKI SDENLIISIK PSDKV 1045
Length:1,045
Mass (Da):121,362
Last modified:January 23, 2007 - v3
Checksum:i5A935B0FE300077E
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M59245 Genomic DNA. Translation: AAA72950.1.
CP001710 Genomic DNA. Translation: ADL59330.1.
RefSeqiYP_003850643.1. NC_014408.1.

Genome annotation databases

EnsemblBacteriaiADL59330; ADL59330; MTBMA_c17620.
GeneIDi9705473.
KEGGimmg:MTBMA_c17620.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M59245 Genomic DNA. Translation: AAA72950.1 .
CP001710 Genomic DNA. Translation: ADL59330.1 .
RefSeqi YP_003850643.1. NC_014408.1.

3D structure databases

ProteinModelPortali P26499.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ADL59330 ; ADL59330 ; MTBMA_c17620 .
GeneIDi 9705473.
KEGGi mmg:MTBMA_c17620.

Phylogenomic databases

HOGENOMi HOG000246403.
KOi K01870.
OMAi KPVHWCL.

Enzyme and pathway databases

BioCyci MMAR79929:GH5J-1768-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02003. Ile_tRNA_synth_type2.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Isoleucyl-tRNA synthetase of Methanobacterium thermoautotrophicum Marburg. Cloning of the gene, nucleotide sequence, and localization of a base change conferring resistance to pseudomonic acid."
    Jenal U., Rechsteiner T., Tan P.-Y., Buehlmann E., Meile L., Leisinger T.
    J. Biol. Chem. 266:10570-10577(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE.
    Strain: DSM 2133 / 14651 / NBRC 100331 / OCM 82 / Marburg.
  2. "Complete genome sequence of Methanothermobacter marburgensis, a methanoarchaeon model organism."
    Liesegang H., Kaster A.K., Wiezer A., Goenrich M., Wollherr A., Seedorf H., Gottschalk G., Thauer R.K.
    J. Bacteriol. 192:5850-5851(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DSM 2133 / 14651 / NBRC 100331 / OCM 82 / Marburg.

Entry informationi

Entry nameiSYI_METTM
AccessioniPrimary (citable) accession number: P26499
Secondary accession number(s): D9PYN2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: January 23, 2007
Last modified: May 14, 2014
This is version 94 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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