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P26491 (CISY_MYCSM) Reviewed, UniProtKB/Swiss-Prot

Last modified October 16, 2013. Version 70. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Citrate synthase

EC=2.3.3.1
Gene names
Name:gltA
OrganismMycobacterium smegmatis
Taxonomic identifier1772 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacterium

Protein attributes

Sequence length375 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

Acetyl-CoA + H2O + oxaloacetate = citrate + CoA.

Enzyme regulation

Allosterically inhibited by NADH.

Pathway

Carbohydrate metabolism; tricarboxylic acid cycle; isocitrate from oxaloacetate: step 1/2.

Subunit structure

Homohexamer.

Miscellaneous

Citrate synthase is found in nearly all cells capable of oxidative metabolism.

Sequence similarities

Belongs to the citrate synthase family.

Ontologies

Keywords
   Biological processTricarboxylic acid cycle
   Molecular functionTransferase
   Technical termAllosteric enzyme
Gene Ontology (GO)
   Biological_processcellular carbohydrate metabolic process

Inferred from electronic annotation. Source: InterPro

tricarboxylic acid cycle

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: InterPro

   Molecular_functioncitrate (Si)-synthase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 375375Citrate synthase
PRO_0000169951

Sites

Active site2661 By similarity
Active site3171 By similarity

Sequences

Sequence LengthMass (Da)Tools
P26491 [UniParc].

Last modified August 1, 1992. Version 1.
Checksum: 95D6D4E0C954C994

FASTA37541,501
        10         20         30         40         50         60 
MTTATESEAP RIHKGLAGVV VDTTAISKVV PETNSLTYRG YPVQDLAAQC SFEQVAYLLW 

        70         80         90        100        110        120 
HGELPTDQLA LFSQRERASR RIDRSMQALL AKLPDNCHPM DVVRTAISYL GAEDLEEDVD 

       130        140        150        160        170        180 
TAEANYAKSL RMFAVLPTIV ATDIRRRQGL TPIPPHSQLG YAQNFLNMCF GEVPEPVVVR 

       190        200        210        220        230        240 
AFEQSMVLYA EHSFNASTFA ARVVTSTQSD IYSAVTAAIG ALKGSLHGGA NEAVMHDMLE 

       250        260        270        280        290        300 
IGSAEKAPEW LHGKLSRKEK VMGFGHRVYK NGDSRVPTMK VALEQVAQVR DGQRWLDIYN 

       310        320        330        340        350        360 
TLESAMFAAT RIKPNLDFPT GPAYYLMDFP IESFTPLFVM SRITGWTAHI MEQAASNALI 

       370 
RPLSEYSGQP QRSLV 

« Hide

References

[1]"Citrate synthase from Mycobacterium smegmatis. Cloning, sequence determination and expression in Escherichia coli."
David M., Lubinsky-Mink S., Benzvi A., Suissa M., Unitzur S., Kuhn J.C.
Biochem. J. 278:225-234(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 607 / DSM 43465 / JCM 20379 / NBRC 3207 / NRRL B-692.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X60513 Genomic DNA. Translation: CAA43028.1.
PIRYKMY. S17168.

3D structure databases

ProteinModelPortalP26491.
SMRP26491. Positions 10-374.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayUPA00223; UER00717.

Family and domain databases

Gene3D1.10.230.10. 1 hit.
1.10.580.10. 1 hit.
InterProIPR011278. 2-MeCitrate/Citrate_synth_I.
IPR016142. Citrate_synth-like_lrg_a-sub.
IPR016143. Citrate_synth-like_sm_a-sub.
IPR002020. Citrate_synthase-like.
IPR016141. Citrate_synthase-like_core.
IPR019810. Citrate_synthase_AS.
IPR024176. Citrate_synthase_bac-typ.
[Graphical view]
PANTHERPTHR11739. PTHR11739. 1 hit.
PfamPF00285. Citrate_synt. 1 hit.
[Graphical view]
PIRSFPIRSF001369. Citrate_synth. 1 hit.
PRINTSPR00143. CITRTSNTHASE.
SUPFAMSSF48256. SSF48256. 1 hit.
TIGRFAMsTIGR01800. cit_synth_II. 1 hit.
PROSITEPS00480. CITRATE_SYNTHASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameCISY_MYCSM
AccessionPrimary (citable) accession number: P26491
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: August 1, 1992
Last modified: October 16, 2013
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways