ID   FIXK_AZOC5              Reviewed;         248 AA.
AC   P26488; A8I013;
DT   01-AUG-1992, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1992, sequence version 1.
DT   27-MAR-2024, entry version 120.
DE   RecName: Full=Nitrogen fixation regulation protein FixK;
GN   Name=fixK; OrderedLocusNames=AZC_4653;
OS   Azorhizobium caulinodans (strain ATCC 43989 / DSM 5975 / JCM 20966 / LMG
OS   6465 / NBRC 14845 / NCIMB 13405 / ORS 571).
OC   Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
OC   Xanthobacteraceae; Azorhizobium.
OX   NCBI_TaxID=438753;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=1766374; DOI=10.1111/j.1365-2958.1991.tb00820.x;
RA   Kaminski P.A., Mandon K., Arigoni F., Desnoues N., Elmerich C.;
RT   "Regulation of nitrogen fixation in Azorhizobium caulinodans:
RT   identification of a fixK-like gene, a positive regulator of nifA.";
RL   Mol. Microbiol. 5:1983-1991(1991).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 43989 / DSM 5975 / JCM 20966 / LMG 6465 / NBRC 14845 /
RC   NCIMB 13405 / ORS 571;
RA   Lee K.B., Backer P.D., Aono T., Liu C.T., Suzuki S., Suzuki T., Kaneko T.,
RA   Yamada M., Tabata S., Kupfer D.M., Najar F.Z., Wiley G.B., Roe B.,
RA   Binnewies T., Ussery D., Vereecke D., Gevers D., Holsters M., Oyaizu H.;
RT   "Complete genome sequence of the nitrogen-fixing bacterium Azorhizobium
RT   caulinodans ORS571.";
RL   Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: FixK is a protein that regulates nitrogen fixation genes both
CC       positively and negatively. FixK is a positive regulator of NifA
CC       expression. FixK may bind DNA at the FNR consensus binding site.
CC   -!- INDUCTION: Oxygen is involved in the regulation of fixK expression.
CC       FixLJ strongly controls the transcription of fixK.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; X59071; CAA41794.1; -; Genomic_DNA.
DR   EMBL; AP009384; BAF90651.1; -; Genomic_DNA.
DR   PIR; S16632; S16632.
DR   RefSeq; WP_012173172.1; NC_009937.1.
DR   AlphaFoldDB; P26488; -.
DR   SMR; P26488; -.
DR   STRING; 438753.AZC_4653; -.
DR   KEGG; azc:AZC_4653; -.
DR   eggNOG; COG0664; Bacteria.
DR   HOGENOM; CLU_075053_0_1_5; -.
DR   Proteomes; UP000000270; Chromosome.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:InterPro.
DR   GO; GO:0009399; P:nitrogen fixation; IEA:UniProtKB-KW.
DR   CDD; cd00038; CAP_ED; 1.
DR   CDD; cd00092; HTH_CRP; 1.
DR   Gene3D; 2.60.120.10; Jelly Rolls; 1.
DR   Gene3D; 1.10.10.10; Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain; 1.
DR   InterPro; IPR000595; cNMP-bd_dom.
DR   InterPro; IPR018490; cNMP-bd_dom_sf.
DR   InterPro; IPR012318; HTH_CRP.
DR   InterPro; IPR014710; RmlC-like_jellyroll.
DR   InterPro; IPR018335; Tscrpt_reg_HTH_Crp-type_CS.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   InterPro; IPR036390; WH_DNA-bd_sf.
DR   PANTHER; PTHR24567; CRP FAMILY TRANSCRIPTIONAL REGULATORY PROTEIN; 1.
DR   PANTHER; PTHR24567:SF75; FUMARATE AND NITRATE REDUCTION REGULATORY PROTEIN; 1.
DR   Pfam; PF00027; cNMP_binding; 1.
DR   Pfam; PF13545; HTH_Crp_2; 1.
DR   PRINTS; PR00034; HTHCRP.
DR   SMART; SM00100; cNMP; 1.
DR   SMART; SM00419; HTH_CRP; 1.
DR   SUPFAM; SSF51206; cAMP-binding domain-like; 1.
DR   SUPFAM; SSF46785; Winged helix' DNA-binding domain; 1.
DR   PROSITE; PS00042; HTH_CRP_1; 1.
DR   PROSITE; PS51063; HTH_CRP_2; 1.
PE   2: Evidence at transcript level;
KW   DNA-binding; Nitrogen fixation; Reference proteome; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..248
FT                   /note="Nitrogen fixation regulation protein FixK"
FT                   /id="PRO_0000100156"
FT   DOMAIN          166..241
FT                   /note="HTH crp-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00387"
FT   DNA_BIND        200..219
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00387"
SQ   SEQUENCE   248 AA;  27104 MW;  9106D34484F42C0C CRC64;
     MSIAASVIAH IAPVPAQAYA HPMPSNRWSE MARGVAADES ARPAQVVAAL GTPAVFARNS
     EIFGDDQVAE NVYVVVSGVV RICKLMGDGR RQIEAFCLPG DAFGWETGER YRFSAEAVSE
     CRLVRVKRSV LFARAGSDPE LACALWALSF AELQRAQEHL LLLGRKTAQE RVGSFLLDLA
     RRSGTTNASH VTEVTLAMSR QDIADFLGLT IETVSRTLTY LEEQGTISLP SSRRVLLRDR
     SALRRLDS
//