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Protein

Nitrogen fixation regulation protein FixK

Gene

fixK

Organism
Azorhizobium caulinodans (strain ATCC 43989 / DSM 5975 / JCM 20966 / NBRC 14845 / NCIMB 13405 / ORS 571)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at transcript leveli

Functioni

FixK is a protein that regulates nitrogen fixation genes both positively and negatively. FixK is a positive regulator of NifA expression. FixK may bind DNA at the FNR consensus binding site.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi200 – 21920H-T-H motifPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Nitrogen fixation, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciACAU438753:GJF3-4718-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Nitrogen fixation regulation protein FixK
Gene namesi
Name:fixK
Ordered Locus Names:AZC_4653
OrganismiAzorhizobium caulinodans (strain ATCC 43989 / DSM 5975 / JCM 20966 / NBRC 14845 / NCIMB 13405 / ORS 571)
Taxonomic identifieri438753 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesXanthobacteraceaeAzorhizobium
ProteomesiUP000000270 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 248248Nitrogen fixation regulation protein FixKPRO_0000100156Add
BLAST

Expressioni

Inductioni

Oxygen is involved in the regulation of fixK expression. FixLJ strongly controls the transcription of fixK.

Interactioni

Protein-protein interaction databases

STRINGi438753.AZC_4653.

Structurei

3D structure databases

ProteinModelPortaliP26488.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini166 – 24176HTH crp-typePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 HTH crp-type DNA-binding domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0664.
HOGENOMiHOG000186462.
KOiK15861.
OMAiESGPTHR.
OrthoDBiEOG6Q5NX8.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
2.60.120.10. 1 hit.
InterProiIPR018490. cNMP-bd-like.
IPR000595. cNMP-bd_dom.
IPR012318. HTH_CRP_2.
IPR014710. RmlC-like_jellyroll.
IPR001808. Tscrpt_reg_HTH_Crp.
IPR018335. Tscrpt_reg_HTH_Crp-type_CS.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00027. cNMP_binding. 1 hit.
PF00325. Crp. 1 hit.
[Graphical view]
PRINTSiPR00034. HTHCRP.
SMARTiSM00100. cNMP. 1 hit.
SM00419. HTH_CRP. 1 hit.
[Graphical view]
SUPFAMiSSF51206. SSF51206. 1 hit.
PROSITEiPS00042. HTH_CRP_1. 1 hit.
PS51063. HTH_CRP_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P26488-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSIAASVIAH IAPVPAQAYA HPMPSNRWSE MARGVAADES ARPAQVVAAL
60 70 80 90 100
GTPAVFARNS EIFGDDQVAE NVYVVVSGVV RICKLMGDGR RQIEAFCLPG
110 120 130 140 150
DAFGWETGER YRFSAEAVSE CRLVRVKRSV LFARAGSDPE LACALWALSF
160 170 180 190 200
AELQRAQEHL LLLGRKTAQE RVGSFLLDLA RRSGTTNASH VTEVTLAMSR
210 220 230 240
QDIADFLGLT IETVSRTLTY LEEQGTISLP SSRRVLLRDR SALRRLDS
Length:248
Mass (Da):27,104
Last modified:August 1, 1992 - v1
Checksum:i9106D34484F42C0C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X59071 Genomic DNA. Translation: CAA41794.1.
AP009384 Genomic DNA. Translation: BAF90651.1.
PIRiS16632.

Genome annotation databases

EnsemblBacteriaiBAF90651; BAF90651; AZC_4653.
KEGGiazc:AZC_4653.
PATRICi21026255. VBIAzoCau17976_4850.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X59071 Genomic DNA. Translation: CAA41794.1.
AP009384 Genomic DNA. Translation: BAF90651.1.
PIRiS16632.

3D structure databases

ProteinModelPortaliP26488.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi438753.AZC_4653.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAF90651; BAF90651; AZC_4653.
KEGGiazc:AZC_4653.
PATRICi21026255. VBIAzoCau17976_4850.

Phylogenomic databases

eggNOGiCOG0664.
HOGENOMiHOG000186462.
KOiK15861.
OMAiESGPTHR.
OrthoDBiEOG6Q5NX8.

Enzyme and pathway databases

BioCyciACAU438753:GJF3-4718-MONOMER.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
2.60.120.10. 1 hit.
InterProiIPR018490. cNMP-bd-like.
IPR000595. cNMP-bd_dom.
IPR012318. HTH_CRP_2.
IPR014710. RmlC-like_jellyroll.
IPR001808. Tscrpt_reg_HTH_Crp.
IPR018335. Tscrpt_reg_HTH_Crp-type_CS.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00027. cNMP_binding. 1 hit.
PF00325. Crp. 1 hit.
[Graphical view]
PRINTSiPR00034. HTHCRP.
SMARTiSM00100. cNMP. 1 hit.
SM00419. HTH_CRP. 1 hit.
[Graphical view]
SUPFAMiSSF51206. SSF51206. 1 hit.
PROSITEiPS00042. HTH_CRP_1. 1 hit.
PS51063. HTH_CRP_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Regulation of nitrogen fixation in Azorhizobium caulinodans: identification of a fixK-like gene, a positive regulator of nifA."
    Kaminski P.A., Mandon K., Arigoni F., Desnoues N., Elmerich C.
    Mol. Microbiol. 5:1983-1991(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Complete genome sequence of the nitrogen-fixing bacterium Azorhizobium caulinodans ORS571."
    Lee K.B., Backer P.D., Aono T., Liu C.T., Suzuki S., Suzuki T., Kaneko T., Yamada M., Tabata S., Kupfer D.M., Najar F.Z., Wiley G.B., Roe B., Binnewies T., Ussery D., Vereecke D., Gevers D., Holsters M., Oyaizu H.
    Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 43989 / DSM 5975 / JCM 20966 / NBRC 14845 / NCIMB 13405 / ORS 571.

Entry informationi

Entry nameiFIXK_AZOC5
AccessioniPrimary (citable) accession number: P26488
Secondary accession number(s): A8I013
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: August 1, 1992
Last modified: July 22, 2015
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.