Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Negative regulator of flagellin synthesis

Gene

flgM

Organism
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Responsible for the coupling of flagellin expression to flagellar assembly by preventing expression of the flagellin genes when a component of the middle class of proteins is defective. It negatively regulates flagellar genes by inhibiting the activity of FliA by directly binding to FliA.

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Repressor

Keywords - Biological processi

Bacterial flagellum biogenesis, Transcription, Transcription regulation

Enzyme and pathway databases

BioCyciSENT99287:GCTI-1181-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Negative regulator of flagellin synthesis
Alternative name(s):
Anti-sigma-28 factor
Gene namesi
Name:flgM
Synonyms:flgR, mviS
Ordered Locus Names:STM1172
OrganismiSalmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Taxonomic identifieri99287 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeSalmonella
ProteomesiUP000001014 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm

  • Note: After completion of the hook-basal body structure, it is exported out of the cell through the completely assembled hook-basal body structures.

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 9797Negative regulator of flagellin synthesisPRO_0000087286Add
BLAST

Proteomic databases

PaxDbiP26477.
PRIDEiP26477.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
fliAP0A2E84EBI-10687647,EBI-10687707
fliSP266096EBI-10687647,EBI-2011519

Protein-protein interaction databases

IntActiP26477. 2 interactions.
STRINGi99287.STM1172.

Structurei

3D structure databases

DisProtiDP00027.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domaini

The N-terminal domain is essential for export and the C-terminal domain possess the FliA binding site.

Sequence similaritiesi

Belongs to the FlgM family.Curated

Phylogenomic databases

eggNOGiCOG2747.
HOGENOMiHOG000265658.
KOiK02398.
OMAiSIDRTQS.
OrthoDBiEOG68DD3J.

Family and domain databases

InterProiIPR007412. FlgM.
[Graphical view]
PfamiPF04316. FlgM. 1 hit.
[Graphical view]
TIGRFAMsiTIGR03824. FlgM_jcvi. 1 hit.

Sequencei

Sequence statusi: Complete.

P26477-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSIDRTSPLK PVSTVQTRET SDTPVQKTRQ EKTSAATSAS VTLSDAQAKL
60 70 80 90
MQPGVSDINM ERVEALKTAI RNGELKMDTG KIADSLIREA QSYLQSK
Length:97
Mass (Da):10,568
Last modified:August 1, 1992 - v1
Checksum:i78CC1779935E7E64
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M74222 Genomic DNA. Translation: AAA27075.1.
U03631 Unassigned DNA. Translation: AAC38036.1.
D25292 Genomic DNA. Translation: BAA04978.1.
AE006468 Genomic DNA. Translation: AAL20102.1.
PIRiA41046.
RefSeqiNP_460143.1. NC_003197.1.
WP_000020893.1. NC_003197.1.

Genome annotation databases

EnsemblBacteriaiAAL20102; AAL20102; STM1172.
GeneIDi1252690.
KEGGistm:STM1172.
PATRICi32380791. VBISalEnt20916_1240.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M74222 Genomic DNA. Translation: AAA27075.1.
U03631 Unassigned DNA. Translation: AAC38036.1.
D25292 Genomic DNA. Translation: BAA04978.1.
AE006468 Genomic DNA. Translation: AAL20102.1.
PIRiA41046.
RefSeqiNP_460143.1. NC_003197.1.
WP_000020893.1. NC_003197.1.

3D structure databases

DisProtiDP00027.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP26477. 2 interactions.
STRINGi99287.STM1172.

Proteomic databases

PaxDbiP26477.
PRIDEiP26477.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAL20102; AAL20102; STM1172.
GeneIDi1252690.
KEGGistm:STM1172.
PATRICi32380791. VBISalEnt20916_1240.

Phylogenomic databases

eggNOGiCOG2747.
HOGENOMiHOG000265658.
KOiK02398.
OMAiSIDRTQS.
OrthoDBiEOG68DD3J.

Enzyme and pathway databases

BioCyciSENT99287:GCTI-1181-MONOMER.

Family and domain databases

InterProiIPR007412. FlgM.
[Graphical view]
PfamiPF04316. FlgM. 1 hit.
[Graphical view]
TIGRFAMsiTIGR03824. FlgM_jcvi. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular characterization of flgM, a gene encoding a negative regulator of flagellin synthesis in Salmonella typhimurium."
    Gillen K.L., Hughes K.T.
    J. Bacteriol. 173:6453-6459(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: LT2.
  2. "Mutation of flgM attenuates virulence of Salmonella typhimurium, and mutation of fliA represses the attenuated phenotype."
    Schmitt C.K., Darnell S.C., Tesh V.L., Stocker B.A.D., O'Brien A.D.
    J. Bacteriol. 176:368-377(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: LT2.
  3. "Sequence analysis of the flgA gene and its adjacent region in Salmonella typhimurium, and identification of another flagellar gene, flgN."
    Kutsukake K., Okada T., Yokoseki T., Iino T.
    Gene 143:49-54(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: LT2.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: LT2 / SGSC1412 / ATCC 700720.
  5. "Molecular dissection of the flagellum-specific anti-sigma factor, FlgM, of Salmonella typhimurium."
    Iyoda S., Kutsukake K.
    Mol. Gen. Genet. 249:417-424(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.

Entry informationi

Entry nameiFLGM_SALTY
AccessioniPrimary (citable) accession number: P26477
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: August 1, 1992
Last modified: May 27, 2015
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.