P26477 (FLGM_SALTY) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Negative regulator of flagellin synthesis

Alternative name(s):
Anti-sigma-28 factor

Gene names

Name:	flgM
Synonyms:	flgR, mviS
Ordered Locus Names:	STM1172

Organism

Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) [Reference proteome] [HAMAP]

Taxonomic identifier

99287 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Salmonella › Salmonella choleraesuis › Salmonella enterica I › Salmonella typhimurium

Protein attributes

Sequence length	97 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Responsible for the coupling of flagellin expression to flagellar assembly by preventing expression of the flagellin genes when a component of the middle class of proteins is defective. It negatively regulates flagellar genes by inhibiting the activity of FliA by directly binding to FliA.
Subcellular location	Cytoplasm. Note: After completion of the hook-basal body structure, it is exported out of the cell through the completely assembled hook-basal body structures.
Domain	The N-terminal domain is essential for export and the C-terminal domain possess the FliA binding site.
Sequence similarities	Belongs to the FlgM family.

Ontologies

Keywords
Biological process	Bacterial flagellum biogenesis Transcription Transcription regulation
Cellular component	Cytoplasm
Molecular function	Repressor
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	flagellum organization Inferred from electronic annotation. Source: UniProtKB-KW negative regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 97	97	Negative regulator of flagellin synthesis		PRO_0000087286

Sequences

	Sequence		Length	Mass (Da)
	P26477 [UniParc]. Last modified August 1, 1992. Version 1. Checksum: 78CC1779935E7E64	FASTA	97	10,568
	10 20 30 40 50 60 MSIDRTSPLK PVSTVQTRET SDTPVQKTRQ EKTSAATSAS VTLSDAQAKL MQPGVSDINM 70 80 90 ERVEALKTAI RNGELKMDTG KIADSLIREA QSYLQSK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular characterization of flgM, a gene encoding a negative regulator of flagellin synthesis in Salmonella typhimurium." Gillen K.L., Hughes K.T. J. Bacteriol. 173:6453-6459(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: LT2.
[2]	"Mutation of flgM attenuates virulence of Salmonella typhimurium, and mutation of fliA represses the attenuated phenotype." Schmitt C.K., Darnell S.C., Tesh V.L., Stocker B.A.D., O'Brien A.D. J. Bacteriol. 176:368-377(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: LT2.
[3]	"Sequence analysis of the flgA gene and its adjacent region in Salmonella typhimurium, and identification of another flagellar gene, flgN." Kutsukake K., Okada T., Yokoseki T., Iino T. Gene 143:49-54(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: LT2.
[4]	"Complete genome sequence of Salmonella enterica serovar Typhimurium LT2." McClelland M., Sanderson K.E., Spieth J., Clifton S.W., Latreille P., Courtney L., Porwollik S., Ali J., Dante M., Du F., Hou S., Layman D., Leonard S., Nguyen C., Scott K., Holmes A., Grewal N., Mulvaney E. , Ryan E., Sun H., Florea L., Miller W., Stoneking T., Nhan M., Waterston R., Wilson R.K. Nature 413:852-856(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: LT2 / SGSC1412 / ATCC 700720.
[5]	"Molecular dissection of the flagellum-specific anti-sigma factor, FlgM, of Salmonella typhimurium." Iyoda S., Kutsukake K. Mol. Gen. Genet. 249:417-424(1995) [PubMed] [Europe PMC] [Abstract] Cited for: CHARACTERIZATION.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M74222 Genomic DNA. Translation: AAA27075.1. U03631 Unassigned DNA. Translation: AAC38036.1. D25292 Genomic DNA. Translation: BAA04978.1. AE006468 Genomic DNA. Translation: AAL20102.1.
PIR	A41046.
RefSeq	NP_460143.1. NC_003197.1.
3D structure databases
DisProt	DP00027.
ModBase	Search...
Protein-protein interaction databases
STRING	99287.STM1172.
Proteomic databases
PaxDb	P26477.
PRIDE	P26477.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAL20102; AAL20102; STM1172.
GeneID	1252690.
KEGG	stm:STM1172.
PATRIC	32380791. VBISalEnt20916_1240.
Phylogenomic databases
eggNOG	COG2747.
HOGENOM	HOG000265658.
KO	K02398.
OMA	GTQDIDM.
ProtClustDB	PRK10810.
Enzyme and pathway databases
BioCyc	SENT99287:GCTI-1181-MONOMER.
Family and domain databases
InterPro	IPR007412. FlgM. [Graphical view]
Pfam	PF04316. FlgM. 1 hit. [Graphical view]
TIGRFAMs	TIGR03824. FlgM_jcvi. 1 hit.
ProtoNet	Search...

Entry information

Entry name

FLGM_SALTY

Accession

Primary (citable) accession number: P26477

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1992
Last sequence update:	August 1, 1992
Last modified:	May 29, 2013
This is version 83 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program