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Protein

Anaerobic sulfite reductase subunit B

Gene

asrB

Organism
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

This enzyme catalyzes the hydrogen sulfide production from sulfite. It is strictly anaerobic. It is regulated by electron acceptors rather than by cysteine.

Cofactori

Protein has several cofactor binding sites:
  • [2Fe-2S] clusterBy similarityNote: Binds 1 [2Fe-2S] cluster per subunit.By similarity
  • FADBy similarityNote: Binds 1 FAD per subunit.By similarity

Pathwayi: sulfite reduction

This protein is involved in the pathway sulfite reduction, which is part of Sulfur metabolism.
View all proteins of this organism that are known to be involved in the pathway sulfite reduction and in Sulfur metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei166NADBy similarity1
Metal bindingi240Iron-sulfur (2Fe-2S)Sequence analysis1
Metal bindingi245Iron-sulfur (2Fe-2S)Sequence analysis1
Metal bindingi248Iron-sulfur (2Fe-2S)Sequence analysis1
Metal bindingi256Iron-sulfur (2Fe-2S)Sequence analysis1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi113 – 129FADBy similarityAdd BLAST17
Nucleotide bindingi168 – 213NADBy similarityAdd BLAST46

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Electron transport, Transport

Keywords - Ligandi

2Fe-2S, FAD, Flavoprotein, Iron, Iron-sulfur, Metal-binding, NAD

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-12545.
UniPathwayiUPA00370.

Names & Taxonomyi

Protein namesi
Recommended name:
Anaerobic sulfite reductase subunit B
Gene namesi
Name:asrB
Ordered Locus Names:STM2549
OrganismiSalmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Taxonomic identifieri99287 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeSalmonella
Proteomesi
  • UP000001014 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001483851 – 272Anaerobic sulfite reductase subunit BAdd BLAST272

Proteomic databases

PaxDbiP26475.
PRIDEiP26475.

Expressioni

Inductioni

By sulfite. Repressed by oxygen.

Interactioni

Subunit structurei

The anaerobic sulfite reductase seems to consist of three subunits.

Protein-protein interaction databases

STRINGi99287.STM2549.

Structurei

3D structure databases

ProteinModelPortaliP26475.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini14 – 105FAD-binding FR-typePROSITE-ProRule annotationAdd BLAST92

Sequence similaritiesi

Belongs to the PyrK family.Curated
Contains 1 FAD-binding FR-type domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG4107SHJ. Bacteria.
COG0543. LUCA.
HOGENOMiHOG000225119.
KOiK16951.
OMAiLSLERNM.
PhylomeDBiP26475.

Family and domain databases

Gene3Di2.10.240.10. 1 hit.
InterProiIPR012165. Cyt_c3_hydrogenase_gsu.
IPR019480. Dihydroorotate_DH_Fe-S-bd.
IPR017927. Fd_Rdtase_FAD-bd.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR017938. Riboflavin_synthase-like_b-brl.
IPR014260. Sulphite_reductase_B.
[Graphical view]
PfamiPF10418. DHODB_Fe-S_bind. 1 hit.
PF00175. NAD_binding_1. 1 hit.
[Graphical view]
PIRSFiPIRSF006816. Cyc3_hyd_g. 1 hit.
SUPFAMiSSF63380. SSF63380. 1 hit.
TIGRFAMsiTIGR02911. sulfite_red_B. 1 hit.
PROSITEiPS51384. FAD_FR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P26475-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSHCSCHDKP QHSLLPAAYR ILSITRHTPL EWNFRVAVDF PAHWGQFVEV
60 70 80 90 100
SLPRVGEAPI SVSDYGDGWI DLLIRNVGKV TSALFTLKEG DNVWLRGCYG
110 120 130 140 150
NGYPVDTLRH KPLLVVAGGT GVAPVKGLMR YFVENPQEIG QLDMILGYKN
160 170 180 190 200
RDCVLYKEEM ATWRGKHNLV LTLDEGEADD RYQIGRVTDR LADMTLSDID
210 220 230 240 250
TMQAIVVGPP IMITFTVKML LQKGLKPEQI WVDYERRMAC SVGKCGHCRM
260 270
GEVYVCTDGP IFNYAVAQRF AD
Length:272
Mass (Da):30,609
Last modified:January 23, 2002 - v2
Checksum:i5C73ACCC200AE302
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti16P → A in AAA99276 (PubMed:1704886).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M57706 Genomic DNA. Translation: AAA99276.1.
AE006468 Genomic DNA. Translation: AAL21443.1.
PIRiB38453.
RefSeqiNP_461484.1. NC_003197.1.
WP_000017587.1. NC_003197.1.

Genome annotation databases

EnsemblBacteriaiAAL21443; AAL21443; STM2549.
GeneIDi1254071.
KEGGistm:STM2549.
PATRICi32383733. VBISalEnt20916_2689.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M57706 Genomic DNA. Translation: AAA99276.1.
AE006468 Genomic DNA. Translation: AAL21443.1.
PIRiB38453.
RefSeqiNP_461484.1. NC_003197.1.
WP_000017587.1. NC_003197.1.

3D structure databases

ProteinModelPortaliP26475.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi99287.STM2549.

Proteomic databases

PaxDbiP26475.
PRIDEiP26475.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAL21443; AAL21443; STM2549.
GeneIDi1254071.
KEGGistm:STM2549.
PATRICi32383733. VBISalEnt20916_2689.

Phylogenomic databases

eggNOGiENOG4107SHJ. Bacteria.
COG0543. LUCA.
HOGENOMiHOG000225119.
KOiK16951.
OMAiLSLERNM.
PhylomeDBiP26475.

Enzyme and pathway databases

UniPathwayiUPA00370.
BioCyciMetaCyc:MONOMER-12545.

Family and domain databases

Gene3Di2.10.240.10. 1 hit.
InterProiIPR012165. Cyt_c3_hydrogenase_gsu.
IPR019480. Dihydroorotate_DH_Fe-S-bd.
IPR017927. Fd_Rdtase_FAD-bd.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR017938. Riboflavin_synthase-like_b-brl.
IPR014260. Sulphite_reductase_B.
[Graphical view]
PfamiPF10418. DHODB_Fe-S_bind. 1 hit.
PF00175. NAD_binding_1. 1 hit.
[Graphical view]
PIRSFiPIRSF006816. Cyc3_hyd_g. 1 hit.
SUPFAMiSSF63380. SSF63380. 1 hit.
TIGRFAMsiTIGR02911. sulfite_red_B. 1 hit.
PROSITEiPS51384. FAD_FR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiASRB_SALTY
AccessioniPrimary (citable) accession number: P26475
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: January 23, 2002
Last modified: November 2, 2016
This is version 119 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.