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P26475

- ASRB_SALTY

UniProt

P26475 - ASRB_SALTY

Protein

Anaerobic sulfite reductase subunit B

Gene

asrB

Organism
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 109 (01 Oct 2014)
      Sequence version 2 (23 Jan 2002)
      Previous versions | rss
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    Functioni

    This enzyme catalyzes the hydrogen sulfide production from sulfite. It is strictly anaerobic. It is regulated by electron acceptors rather than by cysteine.

    Cofactori

    Binds 1 2Fe-2S cluster per subunit.By similarity
    Binds 1 FAD per subunit.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei166 – 1661NADBy similarity
    Metal bindingi240 – 2401Iron-sulfur (2Fe-2S)Sequence Analysis
    Metal bindingi245 – 2451Iron-sulfur (2Fe-2S)Sequence Analysis
    Metal bindingi248 – 2481Iron-sulfur (2Fe-2S)Sequence Analysis
    Metal bindingi256 – 2561Iron-sulfur (2Fe-2S)Sequence Analysis

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi113 – 12917FADBy similarityAdd
    BLAST
    Nucleotide bindingi168 – 21346NADBy similarityAdd
    BLAST

    GO - Molecular functioni

    1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
    2. flavin adenine dinucleotide binding Source: InterPro
    3. metal ion binding Source: UniProtKB-KW
    4. oxidoreductase activity Source: InterPro

    GO - Biological processi

    1. pyrimidine nucleotide biosynthetic process Source: InterPro

    Keywords - Biological processi

    Electron transport, Transport

    Keywords - Ligandi

    2Fe-2S, FAD, Flavoprotein, Iron, Iron-sulfur, Metal-binding, NAD

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-12545.
    SENT99287:GCTI-2563-MONOMER.
    UniPathwayiUPA00370.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Anaerobic sulfite reductase subunit B
    Gene namesi
    Name:asrB
    Ordered Locus Names:STM2549
    OrganismiSalmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
    Taxonomic identifieri99287 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeSalmonella
    ProteomesiUP000001014: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 272272Anaerobic sulfite reductase subunit BPRO_0000148385Add
    BLAST

    Proteomic databases

    PaxDbiP26475.
    PRIDEiP26475.

    Expressioni

    Inductioni

    By sulfite. Repressed by oxygen.

    Interactioni

    Subunit structurei

    The anaerobic sulfite reductase seems to consist of three subunits.

    Protein-protein interaction databases

    STRINGi99287.STM2549.

    Structurei

    3D structure databases

    ProteinModelPortaliP26475.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini14 – 10592FAD-binding FR-typePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the PyrK family.Curated
    Contains 1 FAD-binding FR-type domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0543.
    HOGENOMiHOG000225119.
    KOiK16951.
    OMAiMECGIGH.
    OrthoDBiEOG6JX7JM.
    PhylomeDBiP26475.

    Family and domain databases

    Gene3Di2.10.240.10. 1 hit.
    InterProiIPR012165. Cyt_c3_hydrogenase_gsu.
    IPR019480. Dihydroorotate_DH_Fe-S-bd.
    IPR017927. Fd_Rdtase_FAD-bd.
    IPR008333. OxRdtase_FAD-bd_dom.
    IPR001433. OxRdtase_FAD/NAD-bd.
    IPR017938. Riboflavin_synthase-like_b-brl.
    IPR014260. Sulphite_reductase_B.
    [Graphical view]
    PfamiPF10418. DHODB_Fe-S_bind. 1 hit.
    PF00970. FAD_binding_6. 1 hit.
    PF00175. NAD_binding_1. 1 hit.
    [Graphical view]
    PIRSFiPIRSF006816. Cyc3_hyd_g. 1 hit.
    SUPFAMiSSF63380. SSF63380. 1 hit.
    TIGRFAMsiTIGR02911. sulfite_red_B. 1 hit.
    PROSITEiPS51384. FAD_FR. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P26475-1 [UniParc]FASTAAdd to Basket

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    MSHCSCHDKP QHSLLPAAYR ILSITRHTPL EWNFRVAVDF PAHWGQFVEV    50
    SLPRVGEAPI SVSDYGDGWI DLLIRNVGKV TSALFTLKEG DNVWLRGCYG 100
    NGYPVDTLRH KPLLVVAGGT GVAPVKGLMR YFVENPQEIG QLDMILGYKN 150
    RDCVLYKEEM ATWRGKHNLV LTLDEGEADD RYQIGRVTDR LADMTLSDID 200
    TMQAIVVGPP IMITFTVKML LQKGLKPEQI WVDYERRMAC SVGKCGHCRM 250
    GEVYVCTDGP IFNYAVAQRF AD 272
    Length:272
    Mass (Da):30,609
    Last modified:January 23, 2002 - v2
    Checksum:i5C73ACCC200AE302
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti16 – 161P → A in AAA99276. (PubMed:1704886)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M57706 Genomic DNA. Translation: AAA99276.1.
    AE006468 Genomic DNA. Translation: AAL21443.1.
    PIRiB38453.
    RefSeqiNP_461484.1. NC_003197.1.

    Genome annotation databases

    EnsemblBacteriaiAAL21443; AAL21443; STM2549.
    GeneIDi1254071.
    KEGGistm:STM2549.
    PATRICi32383733. VBISalEnt20916_2689.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M57706 Genomic DNA. Translation: AAA99276.1 .
    AE006468 Genomic DNA. Translation: AAL21443.1 .
    PIRi B38453.
    RefSeqi NP_461484.1. NC_003197.1.

    3D structure databases

    ProteinModelPortali P26475.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 99287.STM2549.

    Proteomic databases

    PaxDbi P26475.
    PRIDEi P26475.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAL21443 ; AAL21443 ; STM2549 .
    GeneIDi 1254071.
    KEGGi stm:STM2549.
    PATRICi 32383733. VBISalEnt20916_2689.

    Phylogenomic databases

    eggNOGi COG0543.
    HOGENOMi HOG000225119.
    KOi K16951.
    OMAi MECGIGH.
    OrthoDBi EOG6JX7JM.
    PhylomeDBi P26475.

    Enzyme and pathway databases

    UniPathwayi UPA00370 .
    BioCyci MetaCyc:MONOMER-12545.
    SENT99287:GCTI-2563-MONOMER.

    Family and domain databases

    Gene3Di 2.10.240.10. 1 hit.
    InterProi IPR012165. Cyt_c3_hydrogenase_gsu.
    IPR019480. Dihydroorotate_DH_Fe-S-bd.
    IPR017927. Fd_Rdtase_FAD-bd.
    IPR008333. OxRdtase_FAD-bd_dom.
    IPR001433. OxRdtase_FAD/NAD-bd.
    IPR017938. Riboflavin_synthase-like_b-brl.
    IPR014260. Sulphite_reductase_B.
    [Graphical view ]
    Pfami PF10418. DHODB_Fe-S_bind. 1 hit.
    PF00970. FAD_binding_6. 1 hit.
    PF00175. NAD_binding_1. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF006816. Cyc3_hyd_g. 1 hit.
    SUPFAMi SSF63380. SSF63380. 1 hit.
    TIGRFAMsi TIGR02911. sulfite_red_B. 1 hit.
    PROSITEi PS51384. FAD_FR. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequence analysis and expression of the Salmonella typhimurium asr operon encoding production of hydrogen sulfide from sulfite."
      Huang C.J., Barrett E.L.
      J. Bacteriol. 173:1544-1553(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: EB303.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: LT2 / SGSC1412 / ATCC 700720.

    Entry informationi

    Entry nameiASRB_SALTY
    AccessioniPrimary (citable) accession number: P26475
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1992
    Last sequence update: January 23, 2002
    Last modified: October 1, 2014
    This is version 109 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3