P26475 (ASRB_SALTY) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Anaerobic sulfite reductase subunit B

Gene names

Name:	asrB
Ordered Locus Names:	STM2549

Organism

Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) [Reference proteome] [HAMAP]

Taxonomic identifier

99287 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Salmonella › Salmonella choleraesuis › Salmonella enterica I › Salmonella typhimurium

Protein attributes

Sequence length	272 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	This enzyme catalyzes the hydrogen sulfide production from sulfite. It is strictly anaerobic. It is regulated by electron acceptors rather than by cysteine.
Cofactor	Binds 1 2Fe-2S cluster per subunit By similarity. Binds 1 FAD per subunit By similarity.
Pathway	Sulfur metabolism; sulfite reduction.
Subunit structure	The anaerobic sulfite reductase seems to consist of three subunits.
Subcellular location	Cytoplasm.
Induction	By sulfite. Repressed by oxygen.
Sequence similarities	Belongs to the PyrK family. Contains 1 FAD-binding FR-type domain.

Ontologies

Keywords
Biological process	Electron transport Transport
Cellular component	Cytoplasm
Ligand	2Fe-2S FAD Flavoprotein Iron Iron-sulfur Metal-binding NAD
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW pyrimidine nucleotide biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	2 iron, 2 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW flavin adenine dinucleotide binding Inferred from electronic annotation. Source: InterPro metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW oxidoreductase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 272	272	Anaerobic sulfite reductase subunit B		PRO_0000148385
Regions
	Domain	14 – 105	92	FAD-binding FR-type
	Nucleotide binding	113 – 129	17	FAD By similarity
	Nucleotide binding	168 – 213	46	NAD By similarity
Sites
	Metal binding	240	1	Iron-sulfur (2Fe-2S) Potential
	Metal binding	245	1	Iron-sulfur (2Fe-2S) Potential
	Metal binding	248	1	Iron-sulfur (2Fe-2S) Potential
	Metal binding	256	1	Iron-sulfur (2Fe-2S) Potential
	Binding site	166	1	NAD By similarity
Experimental info
	Sequence conflict	16	1	P → A in AAA99276. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P26475 [UniParc]. Last modified January 23, 2002. Version 2. Checksum: 5C73ACCC200AE302	FASTA	272	30,609
	10 20 30 40 50 60 MSHCSCHDKP QHSLLPAAYR ILSITRHTPL EWNFRVAVDF PAHWGQFVEV SLPRVGEAPI 70 80 90 100 110 120 SVSDYGDGWI DLLIRNVGKV TSALFTLKEG DNVWLRGCYG NGYPVDTLRH KPLLVVAGGT 130 140 150 160 170 180 GVAPVKGLMR YFVENPQEIG QLDMILGYKN RDCVLYKEEM ATWRGKHNLV LTLDEGEADD 190 200 210 220 230 240 RYQIGRVTDR LADMTLSDID TMQAIVVGPP IMITFTVKML LQKGLKPEQI WVDYERRMAC 250 260 270 SVGKCGHCRM GEVYVCTDGP IFNYAVAQRF AD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence analysis and expression of the Salmonella typhimurium asr operon encoding production of hydrogen sulfide from sulfite." Huang C.J., Barrett E.L. J. Bacteriol. 173:1544-1553(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: EB303.
[2]	"Complete genome sequence of Salmonella enterica serovar Typhimurium LT2." McClelland M., Sanderson K.E., Spieth J., Clifton S.W., Latreille P., Courtney L., Porwollik S., Ali J., Dante M., Du F., Hou S., Layman D., Leonard S., Nguyen C., Scott K., Holmes A., Grewal N., Mulvaney E. , Ryan E., Sun H., Florea L., Miller W., Stoneking T., Nhan M., Waterston R., Wilson R.K. Nature 413:852-856(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: LT2 / SGSC1412 / ATCC 700720.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M57706 Genomic DNA. Translation: AAA99276.1. AE006468 Genomic DNA. Translation: AAL21443.1.
PIR	B38453.
RefSeq	NP_461484.1. NC_003197.1.
3D structure databases
ProteinModelPortal	P26475.
ModBase	Search...
Protein-protein interaction databases
STRING	99287.STM2549.
Proteomic databases
PaxDb	P26475.
PRIDE	P26475.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAL21443; AAL21443; STM2549.
GeneID	1254071.
KEGG	stm:STM2549.
PATRIC	32383733. VBISalEnt20916_2689.
Phylogenomic databases
eggNOG	COG0543.
HOGENOM	HOG000225119.
OMA	CGPPVMF.
ProtClustDB	PRK08221.
Enzyme and pathway databases
BioCyc	MetaCyc:MONOMER-12545. SENT99287:GCTI-2563-MONOMER.
UniPathway	UPA00370.
Family and domain databases
Gene3D	2.10.240.10. 1 hit.
InterPro	IPR012165. Cyt_c3_hydrogenase_gsu. IPR019480. Dihydroorotate_DH_Fe-S-bd. IPR017927. Fd_Rdtase_FAD-bd. IPR008333. OxRdtase_FAD-bd_dom. IPR001433. OxRdtase_FAD/NAD-bd. IPR017938. Riboflavin_synthase-like_b-brl. IPR014260. Sulphite_reductase_B. [Graphical view]
Pfam	PF10418. DHODB_Fe-S_bind. 1 hit. PF00970. FAD_binding_6. 1 hit. PF00175. NAD_binding_1. 1 hit. [Graphical view]
PIRSF	PIRSF006816. Cyc3_hyd_g. 1 hit.
SUPFAM	SSF63380. Riboflavin_synthase_like_b-brl. 1 hit.
TIGRFAMs	TIGR02911. sulfite_red_B. 1 hit.
PROSITE	PS51384. FAD_FR. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ASRB_SALTY

Accession

Primary (citable) accession number: P26475

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1992
Last sequence update:	January 23, 2002
Last modified:	May 29, 2013
This is version 103 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program