P26471 (RFAL_SALTY) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
O-antigen ligase

Gene names

Name:	rfaL
Synonyms:	rfbT, waaL
Ordered Locus Names:	STM3713

Organism

Salmonella typhimurium

Taxonomic identifier

90371 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Salmonella

Protein attributes

Sequence length	404 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Adds the O-antigen on the glucose(II) group of LPS.
Pathway	Bacterial outer membrane biogenesis; LPS core biosynthesis.
Subcellular location	Cell membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the O-antigen polymerase family.

Ontologies

Keywords
Biological process	Lipopolysaccharide biosynthesis
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	lipopolysaccharide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ligase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 404	404	O-antigen ligase		PRO_0000208067
Regions
	Transmembrane	66 – 84	19	Helical; Potential
	Transmembrane	184 – 203	20	Helical; Potential
	Transmembrane	227 – 244	18	Helical; Potential

Sequences

	Sequence		Length	Mass (Da)
	P26471 [UniParc]. Last modified August 1, 1992. Version 1. Checksum: 1B84F7B3C757D2AB	FASTA	404	46,034
	10 20 30 40 50 60 MLTTSLTLNK EKWKPIWNKA LVFLFVATYF LDGITRYKHL IIILMVITAI YQVSRSPKSF 70 80 90 100 110 120 PPLFKNSVFY SVAVLSLILV YSILISPDMK ESFKEFENTV LEGFLLYTLL IPVLLKDETK 130 140 150 160 170 180 ETVAKIVLFS FLTSLGLRCL AESILYIEDY NKGIMPFISY AHRHMSDSMV FLFPALLNIW 190 200 210 220 230 240 LFRKNAIKLV FLVLSAIYLF FILGTLSRGA WLAVLIVGVL WAILNRQWKL IGVGAILLAI 250 260 270 280 290 300 IGALVITQHN NKPDPEHLLY KLQQTDSSYR YTNGTQGTAW ILIQENPIKG YGYGNDVYDG 310 320 330 340 350 360 VYNKRVVDYP TWTFKESIGP HNTILYIWFS AGILGLASLV YLYGAIIRET ASSTLRKVEI 370 380 390 400 SPYNAHLLLF LSFVGFYIVR GNFEQVDIAQ IGIITGFLLA LRNR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning, characterization, and DNA sequence of the rfaLK region for lipopolysaccharide synthesis in Salmonella typhimurium LT2." Maclachlan P.R., Kadam S.K., Sanderson K.E. J. Bacteriol. 173:7151-7163(1991) [PubMed: 1657881] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: LT2.
[2]	"Complete genome sequence of Salmonella enterica serovar Typhimurium LT2." McClelland M., Sanderson K.E., Spieth J., Clifton S.W., Latreille P., Courtney L., Porwollik S., Ali J., Dante M., Du F., Hou S., Layman D., Leonard S., Nguyen C., Scott K., Holmes A., Grewal N., Mulvaney E. , Ryan E., Sun H., Florea L., Miller W., Stoneking T., Nhan M., Waterston R., Wilson R.K. Nature 413:852-856(2001) [PubMed: 11677609] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: LT2 / SGSC1412 / ATCC 700720.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M73826 Genomic DNA. Translation: AAA27206.1. AE006468 Genomic DNA. Translation: AAL22572.1.
PIR	B41317.
RefSeq	NP_462613.1. NC_003197.1.
3D structure databases
ModBase	Search...
Proteomic databases
PRIDE	P26471.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1255237.
GenomeReviews	Gene locus STM3713 in contig AE006468_GR.
KEGG	stm:STM3713.
NMPDR	fig|99287.1.peg.3589.
PATRIC	32386261. VBISalEnt20916_3927.
Phylogenomic databases
HOGENOM	HBG467985.
OMA	NDVYDSV.
ProtClustDB	PRK15487.
Enzyme and pathway databases
BioCyc	STYP99287:STM3713-MONOMER.
Family and domain databases
InterPro	IPR007016. O-antigen_ligase-related. [Graphical view]
KO	K02847.
Pfam	PF04932. Wzy_C. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RFAL_SALTY

Accession

Primary (citable) accession number: P26471

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1992
Last sequence update:	August 1, 1992
Last modified:	January 25, 2012
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program