P26470 (WAAK_SALTY) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Lipopolysaccharide 1,2-N-acetylglucosaminetransferase
EC=2.4.1.56

Gene names

Name:	waaK
Synonyms:	rfaK
Ordered Locus Names:	STM3714

Organism

Salmonella typhimurium

Taxonomic identifier

90371 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Salmonella

Protein attributes

Sequence length	381 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Adds the terminal N-acetyl-D-glucosamine group on the glucose(II) group of LPS.
Catalytic activity	UDP-N-acetyl-D-glucosamine + lipopolysaccharide = UDP + N-acetyl-D-glucosaminyllipopolysaccharide.
Pathway	Bacterial outer membrane biogenesis; LPS core biosynthesis.
Subcellular location	Membrane; Peripheral membrane protein.
Sequence similarities	Belongs to the glycosyltransferase group 1 family. Glycosyltransferase 4 subfamily.

Ontologies

Keywords
Biological process	Lipopolysaccharide biosynthesis
Cellular component	Membrane
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	lipopolysaccharide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	lipopolysaccharide N-acetylglucosaminyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 381	381	Lipopolysaccharide 1,2-N-acetylglucosaminetransferase		PRO_0000080306

Sequences

	Sequence		Length	Mass (Da)
	P26470 [UniParc]. Last modified August 1, 1992. Version 1. Checksum: F60F37FF175372C6	FASTA	381	43,152
	10 20 30 40 50 60 MIKKIIFTVT PIFSIPPRGA AAVETWIYQV AKRLSIPNAI ACIKNAGYPE YNKINDNCDI 70 80 90 100 110 120 HYIGFSKVYK RLFQKWTRLD PLPYSQRILN IRDKVTTQED SVIVIHNSMK LYRQIRERNP 130 140 150 160 170 180 NAKLVMHMHN AFEPELPDND AKIIVPSQFL KAFYEERLPA AAVSIVPNGF CAETYKRNPQ 190 200 210 220 230 240 DNLRQQLNIA EDATVLLYAG RISPDKGILL LLQAFKQLRT LRSNIKLVVV GDPYASRKGE 250 260 270 280 290 300 KAEYQKKVLD AAKEIGTDCI MAGGQSPDQM HNFYHIADLV IVPSQVEEAF CMVAVEAMAA 310 320 330 340 350 360 GKAVLASKKG GISEFVLDGI TGYHLAEPMS SDSIINDINR ALADKERHQI AEKAKSLVFS 370 380 KYSWENVAQR FEEQMKNWFD K

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning, characterization, and DNA sequence of the rfaLK region for lipopolysaccharide synthesis in Salmonella typhimurium LT2." Maclachlan P.R., Kadam S.K., Sanderson K.E. J. Bacteriol. 173:7151-7163(1991) [PubMed: 1657881] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: LT2.
[2]	"Complete genome sequence of Salmonella enterica serovar Typhimurium LT2." McClelland M., Sanderson K.E., Spieth J., Clifton S.W., Latreille P., Courtney L., Porwollik S., Ali J., Dante M., Du F., Hou S., Layman D., Leonard S., Nguyen C., Scott K., Holmes A., Grewal N., Mulvaney E. , Ryan E., Sun H., Florea L., Miller W., Stoneking T., Nhan M., Waterston R., Wilson R.K. Nature 413:852-856(2001) [PubMed: 11677609] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: LT2 / SGSC1412 / ATCC 700720.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M73826 Genomic DNA. Translation: AAA27207.1. AE006468 Genomic DNA. Translation: AAL22573.1.
PIR	C41317.
RefSeq	NP_462614.1. NC_003197.1.
3D structure databases
ProteinModelPortal	P26470.
ModBase	Search...
Protein family/group databases
CAZy	GT4. Glycosyltransferase Family 4.
Proteomic databases
PRIDE	P26470.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1255238.
GenomeReviews	Gene locus STM3714 in contig AE006468_GR.
KEGG	stm:STM3714.
NMPDR	fig|99287.1.peg.3590.
PATRIC	32386263. VBISalEnt20916_3928.
Phylogenomic databases
HOGENOM	HBG467986.
OMA	SAIACIK.
ProtClustDB	PRK15484.
Enzyme and pathway databases
BioCyc	STYP99287:STM3714-MONOMER.
Family and domain databases
InterPro	IPR001296. Glyco_trans_1. [Graphical view]
KO	K03280.
Pfam	PF00534. Glycos_transf_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

WAAK_SALTY

Accession

Primary (citable) accession number: P26470

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1992
Last sequence update:	August 1, 1992
Last modified:	January 25, 2012
This is version 76 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program