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Protein

Cytochrome bd-II ubiquinol oxidase subunit 1

Gene

appC

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

A terminal oxidase that catalyzes quinol-dependent, Na+-independent oxygen uptake. Prefers menadiol over other quinols although ubiquinol was not tested (PubMed:8626304). Generates a proton motive force using protons and electrons from opposite sides of the membrane to generate H2O, transferring 1 proton/electron.4 Publications

Catalytic activityi

2 ubiquinol + O2 + n H+(Side 1) = 2 ubiquinone + 2 H2O + n H+(Side 2).

Cofactori

hemeCuratedNote: May bind up to 3 heme groups per complex.Curated

Enzyme regulationi

Inhibited by cyanide; is more sensitive to cyanide than cytochrome bd-I oxidase.1 Publication

Pathwayi: oxidative phosphorylation

This protein is involved in the pathway oxidative phosphorylation, which is part of Energy metabolism.
View all proteins of this organism that are known to be involved in the pathway oxidative phosphorylation and in Energy metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi19Iron (heme 1 axial ligand)Sequence analysis1
Metal bindingi186Iron (heme 2 axial ligand)Sequence analysis1
Metal bindingi393Iron (heme 2 axial ligand)Sequence analysis1

GO - Molecular functioni

  • electron carrier activity Source: EcoCyc
  • metal ion binding Source: UniProtKB-KW
  • oxidoreductase activity, acting on diphenols and related substances as donors, oxygen as acceptor Source: EcoCyc

GO - Biological processi

  • aerobic electron transport chain Source: EcoCyc
  • oxidative phosphorylation Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Transport

Keywords - Ligandi

Heme, Iron, Metal-binding

Enzyme and pathway databases

BioCyciEcoCyc:APPC-MONOMER.
ECOL316407:JW0960-MONOMER.
MetaCyc:APPC-MONOMER.
UniPathwayiUPA00705.

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome bd-II ubiquinol oxidase subunit 1 (EC:1.10.3.10)
Alternative name(s):
Cytochrome bd-II oxidase subunit I
Gene namesi
Name:appC
Synonyms:cbdA, cyxA
Ordered Locus Names:b0978, JW0960
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11380. appC.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 22CytoplasmicSequence analysisAdd BLAST22
Transmembranei23 – 42HelicalSequence analysisAdd BLAST20
Topological domaini43 – 94PeriplasmicSequence analysisAdd BLAST52
Transmembranei95 – 114HelicalSequence analysisAdd BLAST20
Topological domaini115 – 129CytoplasmicSequence analysisAdd BLAST15
Transmembranei130 – 149HelicalSequence analysisAdd BLAST20
Topological domaini150 – 187PeriplasmicSequence analysisAdd BLAST38
Transmembranei188 – 207HelicalSequence analysisAdd BLAST20
Topological domaini208 – 219CytoplasmicSequence analysisAdd BLAST12
Transmembranei220 – 239HelicalSequence analysisAdd BLAST20
Topological domaini240 – 392PeriplasmicSequence analysisAdd BLAST153
Transmembranei393 – 412HelicalSequence analysisAdd BLAST20
Topological domaini413 – 470CytoplasmicSequence analysisAdd BLAST58
Transmembranei471 – 490HelicalSequence analysisAdd BLAST20
Topological domaini491 – 514PeriplasmicSequence analysisAdd BLAST24

GO - Cellular componenti

  • cytochrome complex Source: EcoCyc
  • integral component of membrane Source: UniProtKB-KW
  • membrane Source: UniProtKB
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

3-fold decreased ubiquinone levels but no change in redox levels of the ubiquinone pool (in aerobically grown minimal medium with glucose).3 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001839221 – 514Cytochrome bd-II ubiquinol oxidase subunit 1Add BLAST514

Post-translational modificationi

The N-terminus is blocked.

Proteomic databases

PaxDbiP26459.
PRIDEiP26459.

Expressioni

Inductioni

Induced when bacterial cultures reach stationary phase; synthesis is triggered by phosphate starvation or a shift from aerobic to anaerobic conditions.1 Publication

Interactioni

Subunit structurei

Heterodimer of subunits I and II.1 Publication

Protein-protein interaction databases

BioGridi4260039. 178 interactors.
DIPiDIP-9119N.
IntActiP26459. 2 interactors.
STRINGi511145.b0978.

Structurei

3D structure databases

ProteinModelPortaliP26459.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105C4M. Bacteria.
COG1271. LUCA.
HOGENOMiHOG000084938.
InParanoidiP26459.
KOiK00425.
OMAiWGRLPKL.
PhylomeDBiP26459.

Family and domain databases

InterProiIPR002585. Cyt-d_ubiquinol_oxidase_su_1.
[Graphical view]
PfamiPF01654. Cyt_bd_oxida_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P26459-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MWDVIDLSRW QFALTALYHF LFVPLTLGLI FLLAIMETIY VVTGKTIYRD
60 70 80 90 100
MTRFWGKLFG INFALGVATG LTMEFQFGTN WSFYSNYVGD IFGAPLAMEA
110 120 130 140 150
LMAFFLESTF VGLFFFGWQR LNKYQHLLVT WLVAFGSNLS ALWILNANGW
160 170 180 190 200
MQYPTGAHFD IDTLRMEMTS FSELVFNPVS QVKFVHTVMA GYVTGAMFIM
210 220 230 240 250
AISAWYLLRG RERNVALRSF AIGSVFGTLA IIGTLQLGDS SAYEVAQVQP
260 270 280 290 300
VKLAAMEGEW QTEPAPAPFH VVAWPEQDQE RNAFALKIPA LLGILATHSL
310 320 330 340 350
DKPVPGLKNL MAETYPRLQR GRMAWLLMQE ISQGNREPHV LQAFRGLEGD
360 370 380 390 400
LGYGMLLSRY APDMNHVTAA QYQAAMRGAI PQVAPVFWSF RIMVGCGSLL
410 420 430 440 450
LLVMLIALVQ TLRGKIDQHR WVLKMALWSL PLPWIAIEAG WFMTEFGRQP
460 470 480 490 500
WAIQDILPTY SAHSALTTGQ LAFSLIMIVG LYTLFLIAEV YLMQKYARLG
510
PSAMQSEQPT QQQG
Length:514
Mass (Da):57,920
Last modified:August 1, 1992 - v1
Checksum:i2D2FBD43429D960D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S63811 Genomic DNA. Translation: AAB20284.1.
U00096 Genomic DNA. Translation: AAC74063.1.
AP009048 Genomic DNA. Translation: BAA35743.1.
PIRiS17958.
RefSeqiNP_415497.1. NC_000913.3.
WP_000263582.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC74063; AAC74063; b0978.
BAA35743; BAA35743; BAA35743.
GeneIDi945585.
KEGGiecj:JW0960.
eco:b0978.
PATRICi32117177. VBIEscCol129921_1012.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S63811 Genomic DNA. Translation: AAB20284.1.
U00096 Genomic DNA. Translation: AAC74063.1.
AP009048 Genomic DNA. Translation: BAA35743.1.
PIRiS17958.
RefSeqiNP_415497.1. NC_000913.3.
WP_000263582.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP26459.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4260039. 178 interactors.
DIPiDIP-9119N.
IntActiP26459. 2 interactors.
STRINGi511145.b0978.

Proteomic databases

PaxDbiP26459.
PRIDEiP26459.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74063; AAC74063; b0978.
BAA35743; BAA35743; BAA35743.
GeneIDi945585.
KEGGiecj:JW0960.
eco:b0978.
PATRICi32117177. VBIEscCol129921_1012.

Organism-specific databases

EchoBASEiEB1354.
EcoGeneiEG11380. appC.

Phylogenomic databases

eggNOGiENOG4105C4M. Bacteria.
COG1271. LUCA.
HOGENOMiHOG000084938.
InParanoidiP26459.
KOiK00425.
OMAiWGRLPKL.
PhylomeDBiP26459.

Enzyme and pathway databases

UniPathwayiUPA00705.
BioCyciEcoCyc:APPC-MONOMER.
ECOL316407:JW0960-MONOMER.
MetaCyc:APPC-MONOMER.

Miscellaneous databases

PROiP26459.

Family and domain databases

InterProiIPR002585. Cyt-d_ubiquinol_oxidase_su_1.
[Graphical view]
PfamiPF01654. Cyt_bd_oxida_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAPPC_ECOLI
AccessioniPrimary (citable) accession number: P26459
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: August 1, 1992
Last modified: November 2, 2016
This is version 127 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Caution

Was originally thought not to translocate protons.1 Publication

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.